RESUMEN
An intestinal alkaline phosphatase-like (Kasahara) isoenzyme has been isolated from the serum of a patient with lung cancer and compared with foetal intestinal alkaline phosphatase from the serum of a premature infant and with adult intestinal phosphatase isolated from serum in the same way. Although the ligand-binding sites of the three enzymes were indistinguishable, the foetal intestinal and Kasahara isoenzymes differed slightly from the adult isoenzyme in heat stability and markedly in electrophoretic mobility and neuraminidase-sensitivity, while themselves being similar in these respects. Neither the Kasahara isoenzyme nor foetal phosphatase reacted with anti-placental phosphatase monoclonal antibodies. These results suggest that the Kasahara isoenzyme corresponds to the reappearance of foetal intestinal alkaline phosphatase, rather than to modification of the adult intestinal isoenzyme.
Asunto(s)
Fosfatasa Alcalina/sangre , Intestinos/enzimología , Isoenzimas/sangre , Neoplasias Pulmonares/enzimología , Adulto , Electroforesis en Gel de Poliacrilamida , Sangre Fetal/enzimología , Calor , HumanosRESUMEN
318 newborn babies were studied at term and after various complications of pregnancy or delivery for serum total bilirubin, alkaline phosphatase (ALP), asparate aminotransferase (AST), alanine aminotransferase (ALT) and glucose-6-phosphate dehydrogenase (G-6-PD). The total bilirubin levels tended to be lower in infants born after various complications of pregnancy or delivery than at term. The mean activity of ALP in the infants was not different from that of normal adults. Its activity decreased by the 5th day of life and was lower in the fetal distress group. The AST activity was much wider than the adult range but ALT activity did not differ from the adult level.