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Methods Mol Biol ; 858: 269-310, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-22684961

RESUMEN

In the field of molecular biology, laboratory information management systems (LIMSs) have been created to track workflows through a process pipeline. For the purposes of DNA barcoding, this workflow involves tracking tissues through extraction, PCR, cycle sequencing, and consensus assembly. Importantly, a LIMS that serves the DNA barcoding community must link required elements for public submissions (e.g., primers, trace files) that are generated in the molecular lab with specimen metadata. Here, we demonstrate an example workflow of a specimen's entry into the LIMS database to the publishing of the specimen's genetic data to a public database using Geneious bioinformatics software. Throughout the process, the connections between steps in the workflow are maintained to facilitate post-processing annotation, structured reporting, and fully transparent edits to reduce subjectivity and increase repeatability.


Asunto(s)
Sistemas de Información en Laboratorio Clínico , Código de Barras del ADN Taxonómico/métodos , Sistemas de Información en Laboratorio Clínico/normas , Biología Computacional , Reacción en Cadena de la Polimerasa , Programas Informáticos
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