RESUMEN
UNLABELLED: Chronic social defeat stress leads to the development of mixed anxiety-depression state, which accompanied by immune deficiency in male mice. Paper aimed to study effects of ronkoleukin on the parameters of cellular immunity in the thymus and spleen and psychoemotional state in these animals. METHODS: Mixed anxiety/depression state was produced by chronic social defeat stress during 20 days in male mice. Roncoleukin (5000 ME/kg, i/p) and saline were chronically injected to depressive mice during 2 weeks without agonistic interactions. After this period subpopulations of lymphocytes in the thymus and spleen were studied in male mice. The animals were also studied in behavioral tests estimating the levels of communicativeness, anxiety and depressiveness. RESULTS: Roncoleukin decreases the number of lymphocytes in the thymus and spleen, and increased the number of lymphocytes in blood and thymus index. Medication increased per cent of CD4+8+ lymphocytes in the thymus and per cent of CD8+ and CD3+25- lymphocytes in the spleen. Roncoleukin induced anxiogenic, stimulative and antidepressive effects. CONCLUSION: Roncoleukin has small efficacy for treatment of immune suppression induced by chronic social defeat stress and has anxiogenic, stimulating and weak antidepressive effects.
Asunto(s)
Antidepresivos/farmacología , Ansiedad/tratamiento farmacológico , Depresión/tratamiento farmacológico , Factores Inmunológicos/farmacología , Interleucina-2/farmacología , Estrés Psicológico/tratamiento farmacológico , Animales , Ansiedad/inmunología , Ansiedad/fisiopatología , Conducta Animal/efectos de los fármacos , Depresión/inmunología , Depresión/fisiopatología , Inmunidad Celular/efectos de los fármacos , Inmunidad Innata/efectos de los fármacos , Huésped Inmunocomprometido , Inmunofenotipificación , Inyecciones Intraperitoneales , Recuento de Linfocitos , Masculino , Ratones , Ratones Endogámicos C57BL , Bazo/efectos de los fármacos , Bazo/inmunología , Estrés Psicológico/inmunología , Estrés Psicológico/fisiopatología , Subgrupos de Linfocitos T/efectos de los fármacos , Subgrupos de Linfocitos T/inmunología , Timo/efectos de los fármacos , Timo/inmunologíaRESUMEN
We studied the effects of melatonin on the status of immune organs and parameters of lipid metabolism in rats with alimentary obesity and parameters of lipid metabolism and immune status in Wistar rats kept on high-fat diet and receiving melatonin solution per os. Melatonin leveled the changes in blood and liver parameters of lipid metabolism, which was paralleled by normalization of cellular composition of immune organs. We conclude that melatonin can be a promising agent for the treatment of lipid metabolism and immune status disorders in alimentary obesity.
Asunto(s)
Factores Inmunológicos/farmacología , Metabolismo de los Lípidos/efectos de los fármacos , Melatonina/farmacología , Obesidad/inmunología , Bazo/efectos de los fármacos , Animales , Colesterol/sangre , Dieta Alta en Grasa/efectos adversos , Evaluación Preclínica de Medicamentos , Femenino , Recuento de Leucocitos , Hígado/efectos de los fármacos , Hígado/metabolismo , Ganglios Linfáticos/efectos de los fármacos , Ganglios Linfáticos/patología , Linfocitos/efectos de los fármacos , Obesidad/sangre , Obesidad/etiología , Ratas Wistar , Bazo/patología , Triglicéridos/sangreRESUMEN
We studied the effects of IL-2 administration at different times of day to female B6D2F1 mice with experimental immunopathology, graft-versus-host chronic reaction induced by the administration of lymphoid cells from parental female DBA/2 mice. Recombinant murine IL-2 was injected subcutaneously at 10.00 (group 1) or 16.00 (group 2) after the first cell transfer. Evening administration of IL-2 in contrast to its morning injection prevented cell proliferation in the thymus and spleen, decreased the number of apoptotic splenocytes, significantly stimulated differentiation processes in the thymus, increased the amount of CD4 (+) 25 (high) thymocytes, and decreased the number of CD4 (+) 27 (low) splenocytes. These findings can serve as a prerequisite for the development of chronotherapeutic schemes for immunocorrection.
Asunto(s)
Proliferación Celular , Enfermedad Injerto contra Huésped/patología , Factores Inmunológicos/administración & dosificación , Interleucina-2/administración & dosificación , Bazo/patología , Timo/patología , Animales , Esquema de Medicación , Femenino , Enfermedad Injerto contra Huésped/tratamiento farmacológico , Enfermedad Injerto contra Huésped/inmunología , Transfusión de Linfocitos , Linfocitos/efectos de los fármacos , Linfocitos/inmunología , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos DBA , Tamaño de los Órganos/efectos de los fármacos , Bazo/efectos de los fármacos , Timo/efectos de los fármacosRESUMEN
We studied the possibility of using anabolic steroid retabolil injections for complex correction of behavioral and immune parameters in rats with genetic predisposition to catalepsy. Subpopulation composition of lymphoid organ and blood cells was compared in rats with genetic predisposition to catalepsy and Wistar rats after retabolil administration. In rats predisposed to catalepsy, retabolil reduced the total content of thymus cells and increased absolute count of CD8 (+) thymocytes. In Wistar rats, retabolil increased the total cell count and the content of CD4 (+) thymocytes, but reduced the number of CD8 (+) cells. Therefore, changes in the subpopulation composition of thymus cells after retabolil administration were opposite in rats with genetic predisposition to catalepsy and Wistar rats. Retabolil injections reduced the severity of catalepsy response in rats with genetic predisposition. However, the time of freezing in Wistar rats significantly increased under these conditions.
Asunto(s)
Anabolizantes/administración & dosificación , Catalepsia/genética , Linfocitos/enzimología , Nandrolona/análogos & derivados , Animales , Catalepsia/tratamiento farmacológico , Catalepsia/inmunología , Predisposición Genética a la Enfermedad , Hidroliasas/metabolismo , Recuento de Leucocitos , Linfocitos/efectos de los fármacos , Masculino , Nandrolona/administración & dosificación , Nandrolona Decanoato , Fenotipo , Ratas , Ratas Wistar , Bazo/efectos de los fármacos , Bazo/inmunología , Succinato Deshidrogenasa/metabolismoRESUMEN
We studied the influence of abnormal illumination regimen on cell composition of the central and peripheral organs of the immune system in ISIAH rats and control WAG rats. In ISIAH rats, 24-h illumination for 14 days led to more pronounced inhibition of cell proliferation and differentiation in the thymus and more pronounced decrease in splenocyte proliferation and T and B cell counts in the spleen in comparison with WAG rats; however, the level of antigen-presenting cells in the spleen of ISIAH increased. We concluded that ISIAH rats are more sensitive to abnormal illumination regimen than WAG rats. Twenty-four-hour illumination was associated with impairments of central differentiation of T cells and activation of systemic inflammation followed by impairment of differentiation regulation, which can aggravate metabolic dysfunctions in these animals.
Asunto(s)
Ritmo Circadiano/inmunología , Hipertensión/patología , Sistema Inmunológico/patología , Bazo/patología , Timo/patología , Animales , Células Presentadoras de Antígenos/inmunología , Células Presentadoras de Antígenos/patología , Células Presentadoras de Antígenos/efectos de la radiación , Antígenos CD/genética , Antígenos CD/inmunología , Linfocitos B/inmunología , Linfocitos B/patología , Linfocitos B/efectos de la radiación , Presión Sanguínea , Diferenciación Celular , Expresión Génica , Hipertensión/inmunología , Sistema Inmunológico/inmunología , Sistema Inmunológico/efectos de la radiación , Inflamación/inmunología , Inflamación/patología , Luz , Masculino , Fotoperiodo , Ratas , Bazo/inmunología , Bazo/efectos de la radiación , Linfocitos T/inmunología , Linfocitos T/patología , Linfocitos T/efectos de la radiación , Timo/inmunología , Timo/efectos de la radiaciónRESUMEN
We studied the effect of bezafibrate on hepatic PPARα activity and immune parameters in hypertensive ISIAH rats in comparison with normotensive WAG rats under conditions of LPS treatment. Bezafibrate increased activity of PPARα in WAG rats, but not in ISIAH rats. As differentiated from WAG rats, bezafibrate produced a potent effect on the content of T cell subpopulations in the thymus and spleen of ISIAH rats. Administration of LPS after injection of bezafibrate caused death of 50% ISIAH animals (but not WAG rats), which was associated with low level of HDL cholesterol and increased triglyceride content. Our results suggest that the hypolipidemic treatment (e.g., bezafibrate) can increase the severity of complications in patients with infectious and inflammatory diseases in association with low level of HDL.
Asunto(s)
Bezafibrato/toxicidad , HDL-Colesterol/sangre , Hipertensión/inmunología , Hipolipemiantes/toxicidad , Inmunosupresores/toxicidad , PPAR alfa/metabolismo , Inmunidad Adaptativa/efectos de los fármacos , Animales , Hipertensión/sangre , Lipopolisacáridos/farmacología , Hígado/efectos de los fármacos , Hígado/inmunología , Hígado/metabolismo , Recuento de Linfocitos , Ratas , Ratas Wistar , Bazo/efectos de los fármacos , Bazo/inmunología , Bazo/patología , Timo/efectos de los fármacos , Timo/inmunología , Timo/patologíaRESUMEN
The effects of LPS from E. coli on DNA-binding activities of PPARα and PPARγ in the liver and immune system parameters of were studied in hypertensive ISIAH rats and normotensive WAG rats. In ISIAH rats characterized by low basal level of PPARα, PPARγ, and HDL, the response of the peripheral immune system compartment to LPS was more pronounced and was not associated with decrease in DNA-binding activities of PPARα observed in WAG. Proinflammatory stimulus did not induce proliferative changes in the thymus of ISIAH rats, which can reflect impaired relationships between the central and peripheral organs of the immune system. The character of regulatory interactions between PPARα and immune cells can differ in various rat strains and depend on initial PPARα activity, HDL level, specific features of immune status, resistance to stress, and hormonal and metabolic background.
Asunto(s)
Hipertensión/metabolismo , Lipopolisacáridos/farmacología , Hígado/metabolismo , PPAR alfa/metabolismo , PPAR gamma/metabolismo , Animales , Proliferación Celular , Hipertensión/inmunología , Lípidos/sangre , Hígado/inmunología , Masculino , Unión Proteica , Ratas , Ratas Wistar , Timo/inmunología , Timo/patologíaRESUMEN
The effects of chronic social defeat stress on the percentage of cells in different phases of the cell cycle and in apoptosis in the thymus and spleen of male mice were studied by the method of flow cytofluorometry. In stressed males, thymus weight decreased, the percent of proliferating thymocytes was significantly lower, and the percentage of G0-G1 cells was higher than in intact males. Stress substantially reduced the percentage of splenocytes in the G0-G1 phase and apoptotic cells, but the percentage of S and G2-M cells and proliferation index significantly increased. Chronic administration of anxiolytic diazepam prevented the majority of the changes in the percentage of cells in different phases of the cell cycle, but apoptosis in the thymus increased under these conditions. Possible association between cell cycle disorders, impairment of cell immunity, and chronic anxiety developing under conditions of long-term social defeat stress is considered.
Asunto(s)
Ansiolíticos/farmacología , Ciclo Celular , Diazepam/farmacología , Bazo/efectos de los fármacos , Estrés Psicológico , Timo/efectos de los fármacos , Animales , Apoptosis , Proliferación Celular , Análisis Factorial , Citometría de Flujo , Masculino , Ratones , Ratones Endogámicos C57BL , Bazo/patología , Timo/patologíaRESUMEN
The aim of this study is to investigate the influence of experimental desynchronosis on the immune system and estimate benzo[a]pyrene (BP) influence on the immune reactivity of mice under the condition of desyncronosis (DS) in comparison with intact organisms. Mice (CBA X C57BL)F1 were kept under constant light during two weeks and natural alternation of darkness and light (control group). Animals of the two groups received benzo[a]pyrene solution in olive oil. The existence of diurnal variation of cell populations of lymphoid organs and blood, humoral immune response to SRBC were revealed. Changes of diurnal dynamics of these parameters and decrease of humoral immune response to SRBC have been observed in animals kept under constant light in comparison with animals kept under standard light conditions. It is likely to indicate the development of internal DS. BP influenced the cellular composition and immune response of intact and DS animals in a different way. So, the changes of light regime lead to development of the internal DS of immune system that influenced its functional capacity and sensitivity to damaging effect of chemical ecological factor.
Asunto(s)
Benzo(a)pireno/toxicidad , Trastornos Cronobiológicos/inducido químicamente , Ritmo Circadiano , Sistema Inmunológico/inmunología , Inmunosupresores/toxicidad , Animales , Benzo(a)pireno/efectos adversos , Trastornos Cronobiológicos/inmunología , Oscuridad , Sistema Inmunológico/efectos de los fármacos , Inmunosupresores/efectos adversos , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Neuroinmunomodulación , Factores de TiempoRESUMEN
The effect of melatonin on disturbed circadian variations in the lymphocyte subpopulation composition of the thymus and inguinal lymph nodes was studied in CBA mice exposed to constant illumination for 14 days. The desynchronizing effect of permanent illumination on the thymus consisted in disappearance of circadian variations in the total number of thymocytes, absolute count of thymocytes, absolute counts of CD8(+) and CD4(+) cells, and in inversion of changes in the absolute counts of CD4(+)8(+) cells from 15.00 to 20.00. In lymph nodes circadian variations in the percentage of CD4(+)+ lymphocytes disappeared, while absolute counts of CD4(+)8(+) and CD8(+) cells changed from 15.00 to 20.00. Melatonin restored circadian dynamics of some parameters mainly in the thymus.
Asunto(s)
Ritmo Circadiano , Ganglios Linfáticos/patología , Melatonina/farmacología , Timo/patología , Animales , Linfocitos T CD4-Positivos/metabolismo , Linfocitos T CD8-positivos/metabolismo , Iluminación , Ganglios Linfáticos/efectos de los fármacos , Ganglios Linfáticos/efectos de la radiación , Linfocitos/metabolismo , Masculino , Melatonina/metabolismo , Ratones , Ratones Endogámicos CBA , Fenotipo , Estimulación Luminosa , Timo/efectos de los fármacos , Timo/efectos de la radiación , Factores de TiempoRESUMEN
Parameters of the immune status of the thymus and spleen in rats with genetic catalepsy were lower compared to those in Wistar rats. Diurnal variations in cell subpopulations of lymphoid organs were different in animals of these strains. Behavioral characteristics and neuroendocrine state in rats with genetic catalepsy were associated with specific changes in the immune system and neuroimmune interactions.