RESUMEN
Under the leadership of Corresponding Member of the Russian Academy of Sciences L.I. Korochkin, the Laboratory of Neurogenetics and Developmental Genetics (Institute of Gene Biology, Russian Academy of Sciences) for many years has been conducting studies of nervous system development, neural cell differentiation, and application of gene and cell technology to cure neurodegenerative diseases. The results of the study initiated by L.I. Korochkin and continued by his scientific successors support the direction of allocation of transgenic neurotrofic factors and heat-shock proteins as neuroprotectors for cell therapy. Potential for usage of promoter of HSP70 heat-shock gene of Drosophila to create transgenic constructs for therapy has been shown. Further improvement of technology of nonvirus transfer for therapeutic genes, as well as production of multicomponent genetic constructs coding several therapeutic factors with synergy effect, would stimulate creation of efficient cell medicals to cure neurodegenerative diseases.
Asunto(s)
Animales Modificados Genéticamente , Técnicas de Cultivo de Célula/métodos , Proteínas de Drosophila/genética , Factores de Crecimiento Nervioso/genética , Animales , Proteínas de Drosophila/metabolismo , Terapia Genética/métodos , Vectores Genéticos , Factor Neurotrófico Derivado de la Línea Celular Glial/genética , Proteínas HSP70 de Choque Térmico/genética , Humanos , Mamíferos/genética , Enfermedad de Parkinson/genética , Enfermedad de Parkinson/terapia , Regiones Promotoras GenéticasRESUMEN
To determine biologically important effects of the cytoplasmic endosymbiont Wolbachia, two substrains of the same Drosophila melanogaster strain have been studied, one of them infected with Wolbachia and the other treated with tetracycline to eliminate the bacterium. Female D. melanogaster infected with Wolbachia are more resistant to the fungus Bauveria bassiana (an insect pathogen) than uninfected females; infected females also exhibited changes in oviposition substrate preference. Males infected with the bacterium are more competitive than uninfected males. The possible role of Wolbachia in the formation of alternative ecological strategies of D. melanogaster is discussed.
Asunto(s)
Antibacterianos/farmacología , Drosophila melanogaster/fisiología , Tetraciclina/farmacología , Wolbachia/fisiología , Animales , Beauveria/fisiología , Drosophila melanogaster/microbiología , Conducta Alimentaria , Femenino , Masculino , Oviposición , Conducta Sexual Animal , Simbiosis , Wolbachia/efectos de los fármacosRESUMEN
Continuous insect cell lines make a special object of research in biology. Insect cells in the established lines differ in the number of attributes from both normal differentiated, and embryonic cells. The period of genome destabilization necessarily precedes cell line immortalization. Genome destabilization is manifested by changes in genome size, cell karyotype, amplification of some retrotransposone families, and induction of their expression. The existence of significant genetic variability in one line puts a problem of searching for invariant attributes providing culture identification and defining the limits of normal polymorphism of cells in the culture. Using the vast collection of insect continuous cell lines stored at the N. I. Vavilov Institute of General Genetics RAS, nine lines were identified by RFLP method of mitochondrial DNA. Variability of DNA-polymorphisms, cellular karyology, morphology, immunological and biochemical attribute in the culture is discussed.