RESUMEN
BACKGROUND: Since some retrospective studies have given inconsistent findings about innervation in adenomyosis, its role in the pain mechanism is still inconclusive. OBJECTIVE: Define the nerve fiber density in adenomyotic tissue as it correlated to pain symptoms. MATERIAL AND METHOD: A cross-sectional study was performed in twenty-five uterine samples from reproductive age women with adenomyosis who underwent either laparotomy or laparoscopic surgery. The nerve fiber density from hysterectomized specimens as measured by immunohistochemistry staining for Protein gene product (PGP) 9.5 and Neurofilament (NF) were compared with the level of pain in the patients as defined by a visual analogue scale and a verbal rating scale. RESULTS: Nerve fibers as detected by PGP9.5 and NF staining in the myometrium were significantly increased in the group of women with adenomyosis experiencing moderate and severe pain as compared to the group experiencing less pain (4 (0, 7) vs. 1.55 (0, 7)/mm2, p-value <0.001, and 6 (3, 10) vs. 0 (0, 4)/mm2, p-value <0.001 respectively). At both phases of the menstrual cycle, the densities of nerve fibers stained with PGP9.5 and NF showed no significant difference. CONCLUSION: These results suggested that the increased of nerve fibers shown in the more severe pain group might play a role in the pathogenesis or symptoms of adenomyosis.
Asunto(s)
Adenomiosis/patología , Miometrio/inervación , Fibras Nerviosas/metabolismo , Dolor/etiología , Adenomiosis/cirugía , Adulto , Estudios Transversales , Femenino , Humanos , Inmunohistoquímica , Laparoscopía/métodos , Laparotomía/métodos , Ciclo Menstrual/fisiología , Persona de Mediana Edad , Dolor/fisiopatología , Dimensión del Dolor , Estudios Retrospectivos , Índice de Severidad de la EnfermedadRESUMEN
BACKGROUND: Tumor angiogenesis has been demonstrated in several kinds of neoplasms. There are evidence that mast cells can produce many kinds of chemical mediators with angiogenic properties. The specific role of mast cells in female genital tract cancer has not been well understood. The purpose of this study was to determine the correlation between the mast cell density, tumor angiogenesis, and clinical outcomes in patients with endometrioid adenocarcinoma of endometrium. METHODS: Histologically, four-micrometer-thick haematoxylin and eosin stained slides of the hysterectomy specimens were evaluated. Microvessels were highlighted by CD31 immunostain and mast cells were stained by 0.1% toluidine blue. All clinicopathological characteristics were reviewed to determine their possible correlation to microvessel density and number of mast cells. RESULTS: A total of 46 patients who underwent a complete staging surgery were eligible for this study. The median age of the patients was 55 years (range, 32-70 years). The median follow-up was 27.0 months (range 3.6-83.8). Microvessel appeared significantly to correlate with the number of parity. The mean microvessel count was likely to be higher in women with non-menopausal status (p=0.07), advanced FIGO stage (p=0.09), and lymph node metastasis (p=0.08). There was no significant correlation between microvessel counts, mast cell density, and disease recurrence. CONCLUSION: Our data suggested that the number of microvessel counts and mast cell density did not affect the clinical progression or recurrence of endometrioid endometrial cancer.
Asunto(s)
Carcinoma Endometrioide/irrigación sanguínea , Carcinoma Endometrioide/patología , Neoplasias Endometriales/irrigación sanguínea , Neoplasias Endometriales/patología , Mastocitos/patología , Neovascularización Patológica , Adulto , Anciano , Recuento de Células , Femenino , Humanos , Metástasis Linfática , Persona de Mediana Edad , Recurrencia Local de Neoplasia/irrigación sanguínea , Recurrencia Local de Neoplasia/patología , PronósticoRESUMEN
BACKGROUND: Tumor angiogenesis has been demonstrated in several kinds of neoplasms. There is evidence that mast cells can produce many different chemical mediators with angiogenic properties. Since their specific role in female genital tract cancer has not been well understood, this study was conducted to determine correlations between among mast cell density, tumor angiogenesis, and clinical outcomes in patients with endometrioid adenocarcinoma of endometrium. METHODS: Histologically, four-micrometer-thick haematoxylin and eosin stained slides of hysterectomy specimens were evaluated. Microvessels were highlighted by CD31 immunostaining and mast cells were stained with 0.1% toluidine blue. All clinicopathological characteristics were reviewed to determine their possible correlation to microvessel density and number of mast cells. RESULTS: A total of 46 patients who underwent a complete staging surgery were eligible for this study. The median age was 55 years (range, 32-70 years) and the median follow-up was 27.0 months (range 3.6-83.8). Microvessels appeared to correlate to some extent with parity and the mean count was likely to be higher in women with non-menopausal status (p=0.07), advanced FIGO stage (p=0.09), and lymph node metastasis (p=0.08). However, there was no significant correlation between microvessel counts, mast cell density, and disease recurrence. CONCLUSION: Our data suggest that the number of microvessel counts and mast cell density do not affect clinical progression or recurrence of endometrioid endometrial cancer.