RESUMEN

The chemical composition of the lipopolysaccharide of the associative diazotrophic rhizobacterium Azospirillum brasilense Cd has been studied. Among the main components of the hydrophobic part of the lipopolysaccharide, we identified 3-hydroxytetradecanoic, hexadecenoic, 3-hydroxyhexadecanoic, hexadecanoic, octadecenoic, and nanodecanoic fatty acids; the carbohydrate part contained rhamnose, galactose, and mannose. Polyclonal antibodies against the preparation under study were raised in rabbits. Serological relations between A. brasilense Cd and other strains of Azospirillum spp. were studied using double radial immunodiffusion and enzyme-linked immunosorbent assay.

Asunto(s)

Azospirillum brasilense/química , Azospirillum brasilense/clasificación , Carbohidratos/análisis , Cromatografía en Gel , Colorimetría , Electroforesis en Gel de Poliacrilamida , Ensayo de Inmunoadsorción Enzimática , Ácidos Grasos/análisis , Inmunodifusión , Lipopolisacáridos/análisis , Serotipificación