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INTRODUCTION: To assess renal and hepatic functions along with the redox state in preeclampsia (PE) and gestational diabetes mellitus (GDM) pregnancies. DESIGN AND METHOD: The study was conducted on 33 PE (mean ageâ¯=â¯30 ± 5 years), 33 GDM (mean ageâ¯=â¯30 ± 6 years) and age, gravida, parity, and ethnicity matched 25 normal pregnancies (NP). Biomarkers of redox status, renal and hepatic functions; aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), bilirubin, urea, blood urea nitrogen (BUN), creatinine, uric acid, lipid peroxidation (MDA), total thiols (TSH), radical scavenging activities and ferric reducing antioxidant potentials of serum were measured. RESULTS: Higher urea, BUN, uric acid, creatinine and overexpressed AST, ALT, ALP was observed in PE (p <0.03-p<0.001), whereas in GDM elevation in creatinine and ALT activity were only statistically significant (p <0.01) in comparison to NP. Diminished antioxidant/radical scavenging potentials, TSH content and increased MDA were noted in both the diseased pregnancies when compared with NP with statistically significant variables ranged from p <0.04-p <0.0001. CONCLUSION: Our data suggest that PE has detrimental effects on renal and hepatic functions while GDM are prone to malfunctioned liver and kidney performances. Impaired redox state may be one of the reasons of altered physiological process in PE and GDM pregnancies. Results may be used to monitor the efficiency and efficacy of antioxidant supplementation, recently suggested for PE and GDM patients.
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Diabetes Gestacional , Riñón/fisiopatología , Hígado/fisiopatología , Preeclampsia , Adulto , Alanina Transaminasa/metabolismo , Aspartato Aminotransferasas/metabolismo , Diabetes Gestacional/metabolismo , Diabetes Gestacional/fisiopatología , Femenino , Humanos , Peroxidación de Lípido , Preeclampsia/metabolismo , Preeclampsia/fisiopatología , Embarazo , Adulto JovenRESUMEN
OBJECTIVES: Piperine (1-piperoyl piperidine), a major alkaloid constituent of Piper nigrum L. and Piper longum L. has pleiotropic biological effects, but the mechanism(s) involved remain to be elucidated. The current study was conducted to examine the efficacy of antioxidant ability of piperine on t-BHP induced markers of oxidative stress in human erythrocytes. METHODS: Healthy human erythrocytes and erythrocytes membrane was stressed with free radical inducer chemical; t-BHP (10-5 M), and the effects of piperine was measured against free radical mediated modification in lipid and protein content, -SH and GSH value with antioxidant potential. RESULTS: The results demonstrate that treatment of erythrocytes with piperine (10-5 to 10-7 M) significantly (p<0.05) ameliorated the adverse consequences of oxidative stress as evidenced by prevention of oxidation of erythrocyte reduced glutathione, membrane thiols, proteins, and peroxidation of lipids; the effects were in correlation with ferric reducing and radical scavenging abilities of piperine. CONCLUSIONS: The study concludes that piperine possesses potent anti-oxidant potential which may explain many of its observed biological effects.
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Alcaloides , Alcaloides/farmacología , Benzodioxoles , Eritrocitos , Humanos , Estrés Oxidativo , Piperidinas/farmacología , Alcamidas PoliinsaturadasRESUMEN
Present study was conducted to evaluate the effect of oral supplementation of composite extract of leaves (CLE) of four medicinal plants; Aegle marmelos, Ocimum sanctum, Murraya koenigii and Azadirachta indica on markers of oxidative stress in brain tissues of alloxan-induced diabetic rats in vivo. Enhanced lipid peroxidation, protein oxidation and reduced antioxidative defence systems were measured in brain tissues of diabetic rats. Supplementation of CLE, once in a day for 35 days significantly (p < .05) protected the peroxidation of lipid, oxidation of protein and ameliorated the antioxidant defence in brain tissue of diabetic rats. It was observed that the insulin-like effect of CLE was dose dependent; higher effect at higher doses. The results of the study suggest that supplementation CLE may provide an overall homeostasis and significant neuro-protection through rescuing brain cells from oxidative abuse and accelerating brain antioxidative defence during advanced stage of hyperglycaemia.
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Antioxidantes/uso terapéutico , Diabetes Mellitus Experimental/dietoterapia , Suplementos Dietéticos , Estrés Oxidativo , Extractos Vegetales/uso terapéutico , Hojas de la Planta/química , Plantas Medicinales/química , Animales , Antioxidantes/administración & dosificación , Biomarcadores/sangre , Biomarcadores/metabolismo , Encéfalo/efectos de los fármacos , Encéfalo/enzimología , Encéfalo/metabolismo , Diabetes Mellitus Experimental/sangre , Diabetes Mellitus Experimental/tratamiento farmacológico , Diabetes Mellitus Experimental/metabolismo , Hiperglucemia/prevención & control , Hipoglucemiantes/administración & dosificación , Hipoglucemiantes/uso terapéutico , India , Insulina Isófana Humana/uso terapéutico , Peroxidación de Lípido/efectos de los fármacos , Masculino , Proteínas del Tejido Nervioso/química , Proteínas del Tejido Nervioso/metabolismo , Neuronas/efectos de los fármacos , Neuronas/enzimología , Neuronas/metabolismo , Fármacos Neuroprotectores/administración & dosificación , Fármacos Neuroprotectores/uso terapéutico , Oxidación-Reducción , Estrés Oxidativo/efectos de los fármacos , Oxidorreductasas/metabolismo , Extractos Vegetales/administración & dosificación , Hojas de la Planta/crecimiento & desarrollo , Plantas Medicinales/crecimiento & desarrollo , Ratas WistarRESUMEN
Alterations at the level of plasma membrane are reported to play an important role in cellular senescence. The present study was undertaken to correlate cellular senescence, membrane transport processes and organismal aging. To achieve this objective activities of membrane linked Na+/K + ATPase (NKA), Na+/H+ exchanger (NHE) and correlation with membrane hydrxyperoxide level, sialic acid content and membrane protein oxidation was studied in density-gradient fractionated young and old erythrocytes from 4 and 24 month old Wistar rats. The results reveal that cellular aging within the tissue is associated with significant decrease in activities of NKA and NHE of senescent erythrocytes in comparison to younger cell population of same age group. The result shows that impaired ion homeostasis due to altered membrane transporters including functional and compositional changes may be one of the reasons responsible behind rat erythrocyte aging.
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Membrana Celular/patología , Senescencia Celular/fisiología , Eritrocitos/patología , Proteínas de la Membrana/metabolismo , Intercambiadores de Sodio-Hidrógeno/metabolismo , ATPasa Intercambiadora de Sodio-Potasio/metabolismo , Animales , Membrana Celular/metabolismo , Eritrocitos/metabolismo , Immunoblotting , Peróxidos Lipídicos/metabolismo , Masculino , Carbonilación Proteica , Ratas , Ratas WistarRESUMEN
An altered ion homeostasis due to impaired membrane transporters is known to be involved in the pathophysiology of many chronic diseases. Resveratrol, a phytoalexin, has been reported to elicit pleiotropic health-promoting effects, however, the mechanism(s) which underlie these effects remain speculative. The present study investigate the modulatory role of resveratrol on erythrocyte membrane Ca(2+)ATPase (PMCA pump), Na(+)/K(+)-ATPase (NKA pump), and Na(+)/H(+) exchanger (NHE) in control and experimental-oxidative stress conditions. Results suggest that resveratrol is a potent modulator of membrane transporters evidenced by stimulation of PMCA and NKA pumps and down-regulation of NHE. The observed effects on membrane transporters correlated with susceptibility of erythrocyte membrane to oxidative damage. The findings provide an insight into the role of membrane transporters and their involvement in the health beneficial effects of resveratrol.
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Eritrocitos/efectos de los fármacos , Proteínas de Transporte de Membrana/metabolismo , Estilbenos/farmacología , Eritrocitos/metabolismo , Humanos , Estrés Oxidativo , ResveratrolRESUMEN
Implication of reactive oxygen species/oxidative stress has been readily reported in etiology of aging and related manifestations. Plasma membrane as a regulator of numerous aspects of cell physiology including cell-cell interaction, solute transport, and signal transduction, provides structural integrity to the cells. The aim of the present study was to determine the effect of resveratrol administration in vitro, to evaluate the biological effect of this phytoalexin in oxidatively injured erythrocytes during aging. This study, carried out on 91 normal healthy subjects, provides experimental evidence that erythrocytes have increased oxidative damage with age. In vitro administration of resveratrol significantly attenuated deleterious effects of oxidative injury in erythrocytes from humans of all ages.
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Membrana Eritrocítica/efectos de los fármacos , Estilbenos/farmacología , Adolescente , Adulto , Factores de Edad , Anciano , Anciano de 80 o más Años , Células Cultivadas , Femenino , Humanos , Masculino , Persona de Mediana Edad , Oxidación-Reducción/efectos de los fármacos , Resveratrol , Adulto Joven , terc-Butilhidroperóxido/efectos adversosRESUMEN
The worldwide incidence of diabetes mellitus has reached alarming proportions. Persistent hyperglycemia due to impaired insulin activity and/or insulin resistance inversely affects the retina, cerebrovascular system, kidney, peripheral limbs, and other parts of the body, which leads to life-threatening complications. The causal role of oxidative stress in the development and progression of diabetic complications has been emphasized. Polyphenols present in natural products have gained much attention in recent decades in preventive studies against diabetes-associated pathologies. In the present review, we provide a comparative update on the role of quercetin, myricetin, and resveratrol-the major polyphenols present in red grapes-in intervening with diabetic complications, and a brief highlight on the molecular mechanisms underlying oxidative stress mediated hyperglycemia.
RESUMEN
Reactive oxygen/nitrogen species (ROS/RNS)-mediated oxidative damage followed by disturbed cellular homeostasis is involved in aging and related consequences. Lipid peroxidation, post-translational modifications of proteins, and an impaired defense system due to increased oxidative stress jeopardize cell fate and functions, resulting in cell senescence. Resveratrol, a natural stilbene, has extensively been reported to elicit a plethora of health-promoting effects. The present study carried out on 97 healthy human subjects (62 males and 35 females) of both sexes provides experimental evidence that resveratrol confers ability to up-regulate the plasma membrane redox system (PMRS) along with ascorbate free radical reductase, a compensatory system operating in the cell to maintain cellular redox state. Furthermore, resveratrol provided significant protection against lipid peroxidation and protein carbonylation and restored the cellular redox homeostasis measured in terms of glutathione (GSH) and sulfhydryl (-SH) group levels during oxidation injury in erythrocytes of different age groups in humans. Findings suggest a possible role of resveratrol in retardation of age-dependent oxidative stress.
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Envejecimiento/sangre , Antioxidantes/farmacología , Membrana Eritrocítica/efectos de los fármacos , Estilbenos/farmacología , Regulación hacia Arriba/efectos de los fármacos , Membrana Eritrocítica/metabolismo , Humanos , Oxidación-Reducción , ResveratrolRESUMEN
The prevalence of diabetes mellitus is rising all over the world. Uncontrolled state of hyperglycemia due to defects in insulin secretion/action leads to a variety of complications including peripheral vascular diseases, nephropathy, neuropathy, retinopathy, morbidity, and/or mortality. Large body of evidence suggests major role of reactive oxygen species/oxidative stress in development and progression of diabetic complications. In the present paper, we have discussed the recent researches on the biomarkers of oxidative stress during type 2 diabetes mellitus.
RESUMEN
Ageing is associated with many physiological and cellular changes, many of which are due to alterations in the plasma membrane. The functions of membrane transporter proteins are crucial for the maintenance of ionic homeostasis between the extra- and intracellular environments. The aim of the present study was to determine the status of erythrocyte membrane transporters, specifically Ca(2+) -ATPases, Na(+) /K(+) -ATPases and the Na(+) /H(+) exchanger (NHE), during ageing in humans. Furthermore, because tea catechins have been reported to possess strong anti-oxidant potential, the study was extended to evaluate the effect of (-)-epicatechin (EC), (-)-epicatechin-3-gallate (ECG), (-)-epigallocatechin (EGC) and (-)-epigallocatechin-3-gallate (EGCG) on these transporters as a function of human age. The study was performed on 97 normal healthy subjects (62 men, 35 women; 16-80 years old). To investigate the effects of tea catechins, subjects were divided into three groups: young (<40 years old; n = 34); middle-aged (40-60 years old; n = 32); and old (>60 years old; n = 31). Erythrocyte ghosts/cell suspension from each group were incubated with ECG, EGCG, EGC and EC (10 µmol/L) for 30 min at 37°C prior to assay. Ageing significantly increased NHE activity and decreased Ca(2+) -ATPase activity. There were no significant changes in Na(+) /K(+) -ATPase activity during the ageing process. (-)-Epigallocatechin-3-gallate, EGC, ECG and EC effectively mitigated the changes in membrane transporter activity in erythrocytes from all age groups; however, the effect was more pronounced in the old age group. We hypothesize that impairment in -bound transporters may be one of the possible mechanisms underlying the pathological events during ageing. A higher intake of catechin-rich food may provide some protection against age-dependent diseases.
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Envejecimiento/metabolismo , Catequina/análogos & derivados , Membrana Eritrocítica/metabolismo , Proteínas de Transporte de Membrana/biosíntesis , Té , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Envejecimiento/efectos de los fármacos , Catequina/farmacología , Catequina/fisiología , Membrana Eritrocítica/efectos de los fármacos , Femenino , Humanos , Masculino , Persona de Mediana Edad , Estrés Oxidativo/efectos de los fármacos , Estrés Oxidativo/fisiología , Adulto JovenRESUMEN
BACKGROUND: Exposure to high concentrations of oxygen radicals, the lack of nucleus and mitochrondria, inability to synthesise new protein and degradation of detoxifying enzymes makes red blood cells (RBCs) uniquely vulnerable to oxidative stress. This review summarizes the changes in biochemical parameters that primarily contribute to alterations in red blood cells during oxidative stress. METHODS: PubMed, Science Direct and Springer online databases and updates from the Indian Council of Medical Research (ICMR). RESULTS AND CONCLUSION: As one of the first cells to be affected by changes in the redox status of the body, alterations in red blood cells are widely used in first step-diagnoses of a number of pathological conditions. The information presented in this review provides an update on biomarkers of redox balance in red blood cells. These biomarkers may be used for assessment of oxidative stress during human health and disease.
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Eritrocitos/metabolismo , Estrés Oxidativo , Animales , Antioxidantes/metabolismo , Biomarcadores/sangre , Hemoglobinas/metabolismo , Humanos , Proteínas de la Membrana/metabolismoRESUMEN
Although curcumin displays several beneficial properties, its medicinal use is limited by its low bioavailability. In the present study we report the antioxidant potentials of two bioconjugates of curcumin with nicotinic acid and picolinic acid: di-O-nicotinoyl curcumin [1,7-bis (4-O nicotinoyl-3-methoxyphenyl)-1,6-heptadiene-3, 5-dione] and di-O-picolinoyl curcumin [1,7-bis (4-O-picolinoyl-3-methoxyphenyl)-1,6-heptadiene-3, 5-dione], in terms of ferric reducing, radical scavenging and beta-carotene bleaching abilities, and comparing the observed activity with that of curcumin. Results demonstrate that both the bioconjugates possess higher antioxidant potentials as evidenced by enhanced ferric reducing, radical scavenging and beta-carotene bleaching abilities, in comparison with curcumin. On the basis of our results we conclude that these bioconjugates of curcumin may be better than curcumin for medicinal and pharmacological applications.
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Antioxidantes/farmacología , Curcumina/análogos & derivados , Depuradores de Radicales Libres/farmacología , Ácidos Nicotínicos/farmacología , Ácidos Picolínicos/farmacología , beta Caroteno/química , Curcumina/farmacología , Compuestos Férricos/química , Oxidación-ReducciónRESUMEN
Resveratrol is one of the most widely studied of all the plant-produced polyphenols and has diverse, beneficial health effects including anti-cancer and cardio-protective effects. Many of the biological actions of this polyphenol have been attributed to its antioxidant properties. Erythrocytes contain a plasma membrane redox system (PMRS), which transfers electrons from intracellular donors (NADH and/or ascorbate (ASC)) to extracellular acceptors. There is evidence that the intracellular ASC donates electrons to extracellular ascorbate free radicals (AFRs) via the PMRS, which encompasses an AFR reductase; such a redox system enables the cells to effectively counteract oxidative processes.We present evidence to show that human erythrocytes take up resveratrol, and once inside the cell, resveratrol can donate electrons to extracellular electron acceptors through the erythrocyte PMRS and AFR reductase. Incubating human erythrocytes with resveratrol (10 µM) caused a significant activation of the PMRS (41%) and AFR reductase (30%) over (basal level) the control; the effect of resveratrol was concentration-dependent. The electron donating ability of resveratrol is slightly less than that observed with quercetin. The role of resveratrol in activating the erythrocyte PMRS and AFR reductase may assume significance in all disease conditions in which there is a decrease in plasma antioxidant potential.
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Antioxidantes/farmacología , Membrana Eritrocítica/efectos de los fármacos , Estilbenos/farmacología , Adulto , Antioxidantes/metabolismo , Ácido Ascórbico/metabolismo , Membrana Eritrocítica/metabolismo , Eritrocitos/metabolismo , Femenino , Radicales Libres/metabolismo , Humanos , Técnicas In Vitro , Masculino , Oxidación-Reducción/efectos de los fármacos , Quercetina/farmacología , Resveratrol , Adulto JovenRESUMEN
Previous studies have indicated that the main molecular characteristic of aging is the progressive accumulation of oxidative damages in cellular macromolecules. Proteins are one of the main molecular targets of age-related oxidative stress, which have been observed during aging process in cellular systems.. Reactive oxygen species (ROS) can lead to oxidation of amino acid side chains, formation of protein-protein cross-linkages, and oxidation of the peptide backbones. In the present study, we report the age-dependent oxidative alterations in biomarkers of plasma protein oxidation: protein carbonyls (PCO), advanced oxidation protein products (AOPPs) and plasma total thiol groups (T-SH) in the Indian population and also correlate these parameters with total plasma antioxidant potential. We show an age dependent decrease in T-SH levels and increase in PCO and AOPPs level. The alterations in the levels of these parameters correlated significantly with the total antioxidant capacity of the plasma. The levels of oxidized proteins in plasma provide an excellent biomarker of oxidative stress due to the relative long half-life of such oxidized proteins.
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Envejecimiento/metabolismo , Antioxidantes/metabolismo , Proteínas Sanguíneas/metabolismo , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Semivida , Humanos , Masculino , Persona de Mediana Edad , Oxidación-Reducción , Estrés Oxidativo , Especies Reactivas de Oxígeno/metabolismo , Compuestos de Sulfhidrilo/metabolismo , Adulto JovenRESUMEN
Aging is an inevitable universal biological process, which can be characterized by a general decline in physiological function with the accumulation of diverse adverse changes and increased probability of death. Among several theories, oxidative stress/free radical theory offers the best mechanistic elucidation of the aging process and other age -related phenomenon. In the present paper , we discuss the aging process and have focused on the importance of some reliable markers of oxidative stress which may be used as biomarkers of the aging process.
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Envejecimiento/metabolismo , Envejecimiento/fisiología , Biomarcadores/metabolismo , Eritrocitos/metabolismo , Estrés Oxidativo/fisiología , Humanos , Modelos BiológicosRESUMEN
Plant polyphenols are known to possess antioxidant activities. In recent years, there have been numerous reports confirming the efficacy of these compounds to improve plasma antioxidant capacity in humans. Current methods to evaluate the antioxidant potential of polyphenols are based on in vitro assay procedures (TEAC, ORAC, FRAP, DPPH). However, the antioxidant potential assessed by these methods does not correlate with the biological activity observed in vivo. Eukaryotic cells display a plasma membrane redox system (PMRS) that transfers electrons from intracellular substrates to extracellular electron acceptors. Here, we describe a method to evaluate the antioxidant potential of plant polyphenols based on their ability to enter the erythrocytes and donate electrons to PMRS. We also present results to show the potentiating effect of quercetin, EGCG, EC and catechin on erythrocyte PMRS activity.
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Antioxidantes/farmacología , Flavonoides/farmacología , Fenoles/farmacología , Plantas/química , Antioxidantes/química , Membrana Eritrocítica/efectos de los fármacos , Membrana Eritrocítica/metabolismo , Flavonoides/química , Humanos , Oxidación-Reducción/efectos de los fármacos , Fenoles/química , PolifenolesRESUMEN
Resveratrol is a natural polyphenolic compound found largely in the skin of red grapes. Growing evidence suggests that resveratrol may play an important role in the prevention of many human diseases. Many of the biological actions of this polyphenol have been attributed to its antioxidant properties. The present study was undertaken to evaluate the effect of resveratrol on intracellular reduced glutathione (GSH) and membrane sulphydryl groups in erythrocytes subjected to oxidative stress in vitro by incubating with t-BHP (10 microm). The study was aimed to test the efficacy of the antioxidant effect of resveratrol on human erythrocytes. Subjecting erythrocytes to oxidative stress (in vitro) by incubating them with t-BHP (10 microm) caused a significant decrease in the intracellular GSH level and membrane -SH content compared with basal values. Incubation of erythrocytes/membranes with resveratrol (1-100 microm final conc) resulted in significant protection against the t-BHP-induced oxidative stress as evidenced by the increase in GSH level and membrane -SH content. It was observed that the effect of resveratrol is dose/concentration and time-dependent. Since resveratrol is naturally present in many fruits and vegetables, a diet rich in resveratrol may provide protection against degenerative diseases.
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Antioxidantes/farmacología , Eritrocitos/efectos de los fármacos , Estrés Oxidativo , Estilbenos/farmacología , Biomarcadores , Células Cultivadas , Membrana Eritrocítica/efectos de los fármacos , Glutatión/metabolismo , Humanos , Resveratrol , terc-ButilhidroperóxidoRESUMEN
OBJECTIVES: To evaluate oxidative stress and the extent of oxidation of plasma proteins in type 2 diabetic patients. DESIGN AND METHODS: Study was carried out on blood from 31 diabetic patients of both sexes (mean age = 58 + or - 7; duration of diabetes 12 + or - 5 years) and healthy age and sex matched normal subjects. Biomarkers of protein oxidation; plasma protein carbonyls (PCO), advanced oxidation protein products (AOPPs) and -SH group and free radical scavenging capacity of plasma was measured. RESULTS: PCO and AOPPS levels were significantly (P<0.005) higher in diabetic patients in comparison to healthy volunteers. Reduced free radical scavenging capacity (P<0.001) and -SH group (P<0.05) was observed in plasma of type 2 diabetic patients. CONCLUSIONS: Our data suggest that diabetics are susceptible to protein oxidation. Oxidative modulation of proteins due to reduced radical scavenging activity of plasma patients may be one of the reasons of altered physiological processes in type 2 diabetic patients.
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Proteínas Sanguíneas/metabolismo , Diabetes Mellitus Tipo 2/sangre , Biomarcadores/sangre , Estudios de Casos y Controles , Femenino , Depuradores de Radicales Libres/metabolismo , Humanos , Masculino , Persona de Mediana Edad , Oxidación-Reducción , Carbonilación ProteicaRESUMEN
Oxidative stress is believed to be a major contributing factor in the development of late complications of diabetes. Many in vitro and in vivo studies have demonstrated that several parameters of red blood cell function and integrity are negatively affected by increased oxidative stress. Plant polyphenols are reported to exert many biological effects due to their antioxidant property. The present study was undertaken to evaluate the antioxidant effect of myricetin on markers of oxidative stress in erythrocytes from type 2 diabetic patients. The study was carried out on blood samples obtained from 23 type 2 diabetic patients and 23 age-matched control subjects. Erythrocytes were subjected to in vitro oxidative stress by incubating with 10(-5) M tert-butyl hydroperoxide (t-BHP). Erythrocyte membrane lipid peroxidation and protein oxidation were measured in terms of malondialdehyde (MDA) and protein carbonyl group levels. The results showed an elevated MDA and protein carbonyl content in diabetic erythrocytes which were further increased after incubation with t-BHP. Myricetin at micromolar concentration significantly (p < 0.01) protected an t-BHP-induced increase in levels of oxidative stress parameters of diabetic erythrocytes.
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Diabetes Mellitus Tipo 2/sangre , Eritrocitos/efectos de los fármacos , Flavonoides/farmacología , Estrés Oxidativo/efectos de los fármacos , Estudios de Casos y Controles , Eritrocitos/metabolismo , Humanos , Peroxidación de Lípido/efectos de los fármacosRESUMEN
Many of the biological actions of resveratrol have been attributed to its antioxidant properties. In this work, we subjected human erythrocytes to in vitro oxidative stress by incubating them with tert-butylhydroperoxide (t-BHP). This caused a significant increase in the malondialdehyde (MDA) level and the protein carbonyl group content above the basal values. The presence of trans-resveratrol at micromolar concentrations in the incubation medium protected the erythrocytes from t-BHP-induced oxidative stress, as evidenced by the decrease in the MDA level and the protein carbonyl group content. The effect of resveratrol was concentration and time-dependent. Our findings help to explain some of the beneficial effects of resveratrol.