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The resolution of the measurement detection and sensitivity of a polarized low coherence interferometer (PLCI) can be pre-engineered by optimizing the key parameters of the birefringent wedge, which is rarely reported. In this work, we introduce a liquid crystal (LC) wedge in the PLCI and use it to demodulate Fabry-Perot (FP) cavity length. The birefringence property of the nematic LC is used to convert the optical path difference (OPD) of the sensor into a spatial distribution. This results in the production of localized interference fringe patterns. The formation of PLCI fringes and the related shift of the interferogram with a variation in the displacement of the FP displacement sensor is explained with reference to the OPD matching between an LC wedge and the FP cavity. The displacement value is demodulated from the obtained fringe pattern by tracking the centroid position of the fringe envelope and also considering the birefringence dispersion. An additional simulation study shows that the spatial position of the interferogram signal coupled with the dispersion coefficient is almost identical to the experimental data. The demodulated results from both the simulation and experimental investigations are found to be consistent with each other and closely agree with the actual cavity length. Further, the possibility to enhance the sensing resolution is examined by modulating the interferogram fringes using an electric field. Compared to birefringent crystals, the LC wedge presented here is found to be advantageous for high precision and tunability of the measurement range, which is useful for robust fiber optic sensing applications.

RESUMEN

We report a new, to the best of our knowledge, approach to determine the refractive indices (RIs) of liquid crystal (LC) systems by considering a double transmission phenomenon of a light beam within the LC medium and the principle of the wedged-cell refractometer. This modified system delivers better spatial resolution of refracted beams in terms of well-resolved spots, compared to the single transmission of a beam in the conventional thin-prism method. The deviation angle obtained in this method is found to be comparatively greater than that of the thin-prism method. The higher values of deviation angle are described using a theoretical model and further experimentally demonstrated for the 5CB nematic LC compound. The variation of the principal RIs (no, ne), and hence the birefringence (Δn) with temperature as well as electric field is investigated for both transmission methods. A relative comparison is carried out between the simulated and standard values obtained from literature. The present technique provides a higher spatial beam resolution to determine the RIs and birefringence of LC systems, which can be very advantageous for different modern photonic applications based on beam shaping and steering.

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Gliomas and other brain tumors have evaded durable therapies, ultimately causing about 20% of all cancer deaths. Tumors are widespread in the brain at time of diagnosis, limiting surgery and radiotherapy effectiveness. Drugs are also poorly effective. Radiotherapy (RT) is limited by dose to normal tissue. However, high-atomic-number elements absorb X-rays and deposit the absorbed dose locally, even doubling (or more) the local dose. Previously we showed that gold nanoparticles (AuNPs) with RT could eradicate some brain tumors in mice and many other preclinical studies confirmed AuNPs as outstanding radioenhancers. However, impediments to clinical translation of AuNPs have been poor clearance, skin discoloration, and cost. We therefore developed iodine nanoparticles (INPs) that are almost colorless, non-toxic, lower cost, and have reasonable clearance, thus overcoming major drawbacks of AuNPs. Here we report the use of iodine nanoparticle radiotherapy (INRT) in treating advanced human gliomas (U87) grown orthotopically in nude mice resulting in a more than a doubling of median life extension compared to RT alone. Significantly, INRT also enhanced the efficacy of chemotherapy when it was combined with the chemotherapeutic agent Doxil, resulting in some longer-term survivors. While ongoing optimization studies should further improve INRT, clinical translation appears promising.

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RESUMEN

Toll-like receptor 2 (TLR2) plays a critical role in host defenses against mycobacterial infections. The R753Q TLR2 polymorphism has been associated with increased incidence of tuberculosis and infections with non-tuberculous mycobacteria in human populations, but the mechanisms by which this polymorphism affects TLR2 signaling are unclear. In this study, we determined the impact of the R753Q TLR2 polymorphism on macrophage sensing of Mycobacterium smegmatis Upon infection with M. smegmatis, macrophages from knock-in mice harboring R753Q TLR2 expressed lower levels of TNF-α, IL-1ß, IL-6, and IL-10 compared with cells from WT mice, but both R753Q TLR2- and WT-derived macrophages exhibited comparable bacterial burdens. The decreased cytokine responses in R753Q TLR2-expressing macrophages were accompanied by impaired phosphorylation of IL-1R-associated kinase 1 (IRAK-1), p38, ERK1/2 MAPKs, and p65 NF-κB, suggesting that the R753Q TLR2 polymorphism alters the functions of the myeloid differentiation primary response protein 88 (MyD88)-IRAK-dependent signaling axis. Supporting this notion, HEK293 cells stably transfected with YFP-tagged R753Q TLR2 displayed reduced recruitment of MyD88 to TLR2, decreased NF-κB activation, and impaired IL-8 expression upon exposure to M. smegmatis Collectively, our results indicate that the R753Q polymorphism alters TLR2 signaling competence, leading to impaired MyD88-TLR2 assembly, reduced phosphorylation of IRAK-1, diminished activation of MAPKs and NF-κB, and deficient induction of cytokines in macrophages infected with M. smegmatis.

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