RESUMEN
Salmonella enterica serovar Paratyphi A (S. Paratyphi A) is a pathogen that can cause enteric fever. According to the recent epidemic trends of typhoid fever, S. Paratyphi A has been the major important causative factor in paratyphoid fever. An effective vaccine for S. Paratyphi A has not been developed, which made it a tricky public health concern. Until now, how S. Paratyphi A interacts with organisms remain unknown. Here using lifespan assay, we found that S. Paratyphi A could infect Caenorhabditis elegans (C. elegans) at 25°C, and attenuate thermotolerance. The immune response of C. elegans was mediated by tir-1, nsy-1, sek-1, pmk-1, mpk-1, skn-1, daf-2 and daf-16, suggesting that S. Paratyphi A could regulate the MAPK and insulin pathways. Furthermore, we observed several phenotypical changes when C. elegans were fed S. Paratyphi A, including an accelerated decline in body movement, reduced the reproductive capacity, shortened spawning cycle, strong preference for OP50, arrested pharyngeal pumping and colonization of the intestinal lumen. The virulence of S. Paratyphi A requires living bacteria and is not mediated by secreting toxin. Using hydrogen peroxide analysis and quantitative RT-PCR, we discovered that S. Paratyphi A could increase oxidative stress and regulate the immune response in C. elegans. Our results sheds light on the infection mechanisms of S. Paratyphi A and lays a foundation for drugs and vaccine development.
Asunto(s)
Proteínas de Caenorhabditis elegans , Fiebre Tifoidea , Vacunas Tifoides-Paratifoides , Animales , Salmonella paratyphi A , Caenorhabditis elegans , Inmunidad , Proteínas de Caenorhabditis elegans/genética , Factores de Transcripción ForkheadRESUMEN
Plastic pollution in the soil ecosystem is currently receiving worldwide attention. However, little is known whether the presence of microplastics (MPs) in soil will affect the environmental behavior of pesticide residues in soil. Here, the effect of the addition of new mulch MPs (New-MPs), aged mulch MPs (Aged-MPs) and biodegradable mulch MPs (BioD-MPs) on the adsorption and degradation behaviors of two pesticides (imidacloprid and flumioxazin) in soil was investigated. Three MPs slowed down rapid adsorption stage of pesticides in soil and delayed the time to reach adsorption equilibrium. Adsorption rates: Soil > Soil + New-MPs > Soil + Aged-MPs > Soil + BioD-MPs. Three MPs enhanced the adsorption strength of the soil system for the two pesticides, and the aging treatment of the MPs enhanced this effect. Three MPs affected the degradation process of the two pesticides. New-MPs promoted the degradation of two pesticides imidacloprid and flumioxazin, and the degradation half-lives were shortened to 0.93 and 0.85 times, respectively; while Aged-Mps and BioD-MPs delayed the degradation process of two pesticides, and the degradation half-lives were extended to 1.64 times and 1.21 times, respectively. The effect was more significant with the increase of MPs and pesticides concentration. Pesticide polarity, surface structure and functional groups of MPs are potentially important reasons for the differences in adsorption and degradation of MPs-soil systems. Our findings provide a deep insight into understanding the mechanism of interaction between MPs and pesticide residues in soil environment.
Asunto(s)
Residuos de Plaguicidas , Plaguicidas , Contaminantes del Suelo , Adsorción , Ecosistema , Microplásticos , Residuos de Plaguicidas/análisis , Plaguicidas/análisis , Plásticos , Suelo/química , Contaminantes del Suelo/análisisRESUMEN
Aphis gossypii and Helicoverpa armigera are two important agricultural pests in cotton plants. However, whether early colonization of A. gossypii affects subsequent H. armigera is unknown. We implemented ecological experiments to reveal that A. gossypii-damaged cotton plants [Bacillus thuringiensis (Bt) and non-Bt] had a significant avoidance effect on the oviposition preference of H. armigera adults. However, A. gossypii-damaged cotton plants (non-Bt) increased the weight and pupation rate and reduced the mortality of H. armigera larvae. Transcriptomic and metabolomic analyses showed that 13 and 9 genes were significantly upregulated to be involved in salicylic acid (SA) and indole acetic acid (IAA) biosynthesis, and SA and IAA contents were significantly increased, respectively. However, 15 genes involved in jasmonic acid (JA) biosynthesis were significantly downregulated as a result of the antagonism of SA and JA. Moreover, there was significant upregulation in multiple genes involved in the biosynthesis of l-histidine, fructose, maltotetraose, melezitose, lecithin, stearidonic acid, and mannitol, in which metabolites were confirmed to promote the growth and development of H. armigera. Our study is a reference for investigating the evolutionary relationships and provides insights into implementing effective insect biocontrol between H. armigera and A. gossypii.