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To further understand the impact of deficiency of the autoimmune regulator (Aire) gene during the adhesion of medullary thymic epithelial cells (mTECs) to thymocytes, we sequenced single-cell libraries (scRNA-seq) obtained from Aire wild-type (WT) (Airewt/wt ) or Aire-deficient (Airewt/mut ) mTECs cocultured with WT single-positive (SP) CD4+ thymocytes. Although the libraries differed in their mRNA and long noncoding RNA (lncRNA) profiles, indicating that mTECs were heterogeneous in terms of their transcriptome, UMAP clustering revealed that both mTEC lines expressed their specific markers, i.e., Epcam, Itgb4, Itga6, and Casp3 in resting mTECs and Ccna2, Pbk, and Birc5 in proliferative mTECs. Both cocultured SP CD4+ thymocytes remained in a homogeneous cluster expressing the Il7r and Ccr7 markers. Comparisons of the two types of cocultures revealed the differential expression of mRNAs that encode transcription factors (Zfpm2, Satb1, and Lef1), cell adhesion genes (Itgb1) in mTECs, and Themis in thymocytes, which is associated with the regulation of positive and negative selection. At the single-cell sequencing resolution, we observed that Aire acts on both Aire WT and Aire-deficient mTECs as an upstream controller of mRNAs, which encode transcription factors or adhesion proteins that, in turn, are posttranscriptionally controlled by lncRNAs, for example, Neat1, Malat1, Pvt1, and Dancr among others. Under Aire deficiency, mTECs dysregulate the expression of MHC-II, CD80, and CD326 (EPCAM) protein markers as well as metabolism and cell cycle-related mRNAs, which delay the cell cycle progression. Moreover, when adhered to mTECs, WT SP CD4+ or CD8+ thymocytes modulate the expression of cell activation proteins, including CD28 and CD152/CTLA4, and the expression of cellular metabolism mRNAs. These findings indicate a complex mechanism through which an imbalance in Aire expression can affect mTECs and thymocytes during adhesion.
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Proteína AIRE , Adhesión Celular , Células Epiteliales , ARN Largo no Codificante , Timocitos , Factores de Transcripción , Transcriptoma , ARN Largo no Codificante/genética , Animales , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Ratones , Timocitos/metabolismo , Timocitos/inmunología , Timocitos/citología , Células Epiteliales/metabolismo , Células Epiteliales/inmunología , Timo/citología , Timo/inmunología , Timo/metabolismo , Análisis de la Célula Individual , Redes Reguladoras de Genes , Linfocitos T CD4-Positivos/inmunología , Linfocitos T CD4-Positivos/metabolismo , Técnicas de Cocultivo , Perfilación de la Expresión Génica , Ratones NoqueadosRESUMEN
OBJECTIVES: To isolate cancer stem cells (CSC) from a metastatic oral squamous cell carcinoma (OSCC) cell line and investigate their in vitro and in vivo phenotypic characteristics. MATERIALS AND METHODS: Subpopulations with individual staining intensities for CD44 and CD326 were isolated from the OSCC cell line LN-1A by FACS: CD44Low/CD326- (CSC-M1), CD44Low/CD326High (CSC-E), and CD44High/CD326- (CSC-M2). Proliferation, clonogenic potential, adhesion, migration, epithelial-mesenchymal transition markers, and sensitivity to cisplatin and TVB-3166 were analyzed in vitro. Tumor formation and metastasis were assessed by subcutaneous and orthotopic inoculations into BALB/c mice. RESULTS: E-cadherin levels were higher in CSC-E cells while vimentin and Slug more produced by CSC-M2 cells. CSC-M1 and CSC-M2 subpopulations showed higher proliferation, produced more colonies, and have stronger adhesion to the extracellular matrix. All cell lines established tumors; however, CSC-E and CSC-M2 formed larger masses and produced more metastases. CONCLUSION: The CSC subpopulations here described show increased cancer capabilities in vitro, tumorigenic and metastatic potential in vivo, and may be exploited in the search for novel therapeutic targets for OSCC.
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BACKGROUND AND OBJECTIVES: Bone marrow mesenchymal stromal cells (BM-MSCs) are key elements of the hematopoietic niche and participate in the regulatory mechanisms of hematopoietic stem cells (HSCs). Hematological diseases can affect MSCs and their functions. However, the dysregulations caused by sickle cell disease (SCD) are not fully elucidated. This work explored changes in BM-MSCs and their relationship with age using sickle cell mice (Townes-SS). MATERIALS AND METHODS: BM-MSCs were isolated from Townes-SS, and control groups 30- and 60-day-old Townes-AA and C57BL/6 J. RESULTS: The BM-MSCs showed no morphological differences in culture and demonstrated a murine MSC-like immunophenotypic profile (Sca-1+, CD29+, CD44+, CD90.2+, CD31-, CD45-, and CD117-). Subsequently, all BM-MSCs were able to differentiate into adipocytes and osteocytes in vitro. Finally, 30-day-old BM-MSCs of Townes-SS showed higher expression of genes related to the maintenance of HSCs (Cxcl12, Vegfa, and Angpt1) and lower expression of pro-inflammatory genes (Tnfa and Il-6). However, 60-day-old BM-MSCs of Townes-SS started to show expression of genes related to reduced HSC maintenance and increased expression of pro-inflammatory genes. CONCLUSION: These results indicates age as a modifying factor of gene expression of BM-MSCs in the context of SCD.
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Anemia de Células Falciformes , Células Madre Mesenquimatosas , Humanos , Animales , Ratones , Médula Ósea , Ratones Endogámicos C57BL , Células Madre Hematopoyéticas/metabolismo , Células Madre Mesenquimatosas/metabolismo , Células de la Médula Ósea/metabolismo , Diferenciación CelularRESUMEN
Philadelphia-negative myeloproliferative neoplasms (MPN) are clonal hematological diseases associated with driver mutations in JAK2, CALR, and MPL genes. Moreover, several evidence suggests that chronic inflammation and alterations in stromal and immune cells may contribute to MPN's pathophysiology. We evaluated the frequency and the immunophenotype of peripheral blood monocyte subpopulations in patients with polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (MF). Peripheral blood monocytes from PV (n = 16), ET (n = 16), and MF (n = 15) patients and healthy donors (n = 10) were isolated and submitted to immunophenotyping to determine the frequency of monocyte subpopulations and surface markers expression density. Plasma samples were used to measure the levels of soluble CD163, a biomarker of monocyte activity. PV, ET, and MF patients presented increased frequency of intermediate and non-classical monocytes and reduced frequency of classical monocytes compared to controls. Positivity for JAK2 mutation was significantly associated with the percentage of intermediate monocytes. PV, ET, and MF patients presented high-activated monocytes, evidenced by higher HLA-DR expression and increased soluble CD163 levels. The three MPN categories presented increased frequency of CD56+ aberrant monocytes, and PV and ET patients presented reduced frequency of CD80/86+ monocytes. Therefore, alterations in monocyte subpopulations frequency and surface markers expression pattern may contribute to oncoinflammation and may be associated with the pathophysiology of MPN.
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Trastornos Mieloproliferativos , Neoplasias , Frecuencia de los Genes , Humanos , Inmunofenotipificación , Monocitos , Trastornos Mieloproliferativos/genéticaRESUMEN
The coronavirus pandemic has exposed a global tendency throughout history to blame immigrants for propagating epidemics. Chinese individuals were thus targeted during past public health crises in Peru, but during the current coronavirus pandemic racist notions painting people of Chinese descent as "agents of contagion" diminished significantly. Here we examine three major epidemics (yellow fever, the bubonic plague, and covid-19) to demonstrate the current and somewhat surprising shift in negative attitudes toward the Chinese community. Peruvians' refusal to embrace derogatory terms (the "Chinese virus") or target individuals of Asian descent constitutes an intriguing case at a moment when xenophobic discourse is rampant in the Western hemisphere.
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COVID-19 , Racismo , COVID-19/epidemiología , China/epidemiología , Migración Humana , Humanos , Pandemias , Perú/epidemiologíaRESUMEN
Abstract The coronavirus pandemic has exposed a global tendency throughout history to blame immigrants for propagating epidemics. Chinese individuals were thus targeted during past public health crises in Peru, but during the current coronavirus pandemic racist notions painting people of Chinese descent as "agents of contagion" diminished significantly. Here we examine three major epidemics (yellow fever, the bubonic plague, and covid-19) to demonstrate the current and somewhat surprising shift in negative attitudes toward the Chinese community. Peruvians' refusal to embrace derogatory terms (the "Chinese virus") or target individuals of Asian descent constitutes an intriguing case at a moment when xenophobic discourse is rampant in the Western hemisphere.
Resumo A pandemia do coronavírus expôs uma tendência mundial ao longo da história de culpar imigrantes pela propagação de epidemias. No Peru, os chineses sofreram preconceito racial durante crises de saúde pública anteriores. Surpreendentemente, a ideia de que os descendentes de chineses são "agentes de contágio" diminuiu significativamente durante a atual pandemia. Examinaremos aqui três grandes epidemias (febre amarela, peste bubônica e covid-19), a fim de demonstrar a atual mudança nas atitudes negativas em relação à comunidade chinesa. A recusa dos peruanos de adotar termos depreciativos (o "vírus chinês") ou visar indivíduos de ascendência asiática é algo intrigante em tempos de completa disseminação de discurso xenofóbico no hemisfério ocidental.
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Prejuicio , Enfermedades Transmisibles , Emigrantes e Inmigrantes , COVID-19 , Perú , Historia del Siglo XXIRESUMEN
Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is the only currently available curative treatment for sickle cell disease (SCD). However, the effects of HSCT on SCD pathophysiology are poorly elucidated. Here, we assessed red blood cell (RBC) adhesiveness, intensity of hemolysis, vascular tone markers and systemic inflammation, in SCD patients treated with allogeneic HSCT. Thirty-two SCD patients were evaluated before and on long-term follow-up after HSCT. Overall survival was 94% with no severe (grade III-IV) graft-vs-host disease and a 22% rejection rate (graft failure). Hematological parameters, reticulocyte counts, and levels of lactate dehydrogenase (LDH), endothelin-1 and VCAM-1 normalized in SCD patients post-HSCT. Expression of adhesion molecules on reticulocytes and RBC was lower in patients with sustained engraftment. Levels of IL-18, IL-15 and LDH were higher in patients that developed graft failure. Increased levels of plasma pro-inflammatory cytokines, mainly TNF-α, were found in SCD patients long-term after transplantation. SCD patients with sustained engraftment after allo-HSCT showed decreased reticulocyte counts and adhesiveness, diminished hemolysis, and lower levels of vascular tonus markers. Nevertheless, systemic inflammation persists for at least five years after transplantation, indicating that allo-HSCT does not equally affect all aspects of SCD pathophysiology.
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Anemia de Células Falciformes/complicaciones , Susceptibilidad a Enfermedades , Inflamación/etiología , Adolescente , Adulto , Anemia de Células Falciformes/diagnóstico , Anemia de Células Falciformes/terapia , Biomarcadores , Recuento de Células Sanguíneas , Niño , Femenino , Trasplante de Células Madre Hematopoyéticas/efectos adversos , Trasplante de Células Madre Hematopoyéticas/métodos , Hemólisis , Humanos , Inflamación/diagnóstico , Mediadores de Inflamación , Masculino , Óxido Nítrico/metabolismo , Factores de Tiempo , Trasplante Homólogo , Adulto JovenRESUMEN
Introducción: el sistema organizacional genera un determinado clima de trabajo que repercute en las motivaciones de los miembros de la organización y en su comportamiento, por lo que al evaluar el clima organizacional se mide la forma como es percibida la organización. Objetivo: identificar el clima organizacional en el personal de enfermería de la unidad de cuidados intensivos (UCI) del Hospital de Especialidades (HE) No. 14 del Instituto Mexicano del Seguro Social (IMSS), en Veracruz, México. Metodología: estudio observacional, descriptivo y transversal, en el que participaron 49 enfermeras de los turnos matutino, vespertino y nocturno de la UCI del HE No. 14. Durante junio y julio de 2019, se aplicó el instrumento Clima Organizacional de Litwin y Stringer, que contiene 53 ítems en sus 9 dimensiones. Resultados: el nivel promedio global del clima organizacional en la UCI fue de 26.4, lo que lleva a categorizarlo como bueno. Las dimensiones: recompensa, estándares y conflicto obtuvieron una puntuación de 23.5 a 24.8, que se traduce como un promedio regular del clima organizacional. Conclusiones: hay un clima organizacional bueno en el personal de enfermería. El componente que influye de manera positiva es la dimensión cooperación, ya que el trabajador empatiza con sus compañeros de servicio. La dimensión conflicto influye de manera negativa, pues abarca la falta de motivación del personal para realizar su trabajo.
Introduction: Organizational systems generate certain workplace environment that affects the staff members' motivation and their behavior, which is why assessing the organizational environment allows to measure the staff perception on the organization. Objective: To identify the organizational environment of the nursing staff at the Specialty Hospital (SH) No. 14 intensive care unit (ICU) of the National Institute for Social Security (Instituto Mexicano del Seguro Social), in Veracruz, Mexico. Methods: Observational, descriptive, and cross-sectional study, which included 49 nurses from the morning, afternoon and night shifts of the aforementioned ICU. During June and July 2019, it was administered the instrument Organizational Climate by Litwin and Stringer, which contains 53 items in its 9 dimensions. Results: The global average level of the organizational environment in the ICU was 26.4, which leads to categorize it as good. The dimensions reward, standards and conflict obtained a range from 23.5 to 24.8, i.e., a regular average of the organizational environment. Conclusions: There is an organizational environment classified as good in the nursing staff. The component that has a positive influence is the cooperation dimension, since the worker empathizes with his fellow employees. The conflict dimension has a negative influence, given that it includes the lack of motivation of the staff to carry out their work.
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Humanos , Masculino , Femenino , Estudios Transversales , Unidades de Cuidados Intensivos , Atención Terciaria de Salud , México , Enfermeras y EnfermerosRESUMEN
This article aims to provide researchers and students with information on digital archives in libraries in Latin America and elsewhere. It is aimed at those interested in health history, focusing on Peru and Chile.
Este artículo tiene como propósito poner al alcance de investigadores y estudiantes información sobre archivos digitales de bibliotecas en América Latina y en el extranjero. Su contenido está orientado para aquellos interesados en historia de la salud, con énfasis en Perú y Chile.
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Archivos , Bibliotecas Digitales , COVID-19 , Chile , Historia de la Medicina , América Latina , Bibliotecas , PerúRESUMEN
Resumen Este artículo tiene como propósito poner al alcance de investigadores y estudiantes información sobre archivos digitales de bibliotecas en América Latina y en el extranjero. Su contenido está orientado para aquellos interesados en historia de la salud, con énfasis en Perú y Chile.
Abstract This article aims to provide researchers and students with information on digital archives in libraries in Latin America and elsewhere. It is aimed at those interested in health history, focusing on Peru and Chile.
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Archivos , Bibliotecas Digitales , Perú , Chile , COVID-19 , Historia de la Medicina , América Latina , BibliotecasRESUMEN
Introducción: la epidemia de covid-19 ha dejado en evidencia una serie de problemas de desigualdad en el acceso a la salud pública en Perú, especialmente en las provincias alejadas de la capital, donde se observa precariedad tanto en infraestructura como en personal sanitario. Este artículo analiza cómo el Gobierno y la población han hecho frente a epidemias en el sur de Perú, usando como casos de estudio el covid-19 y la peste bubónica ocurrida en 1903 y 1905, con el fin de entender continuidades en el manejo de la crisis epidémica y su respuesta a ellas. Desarrollo: se estudia la epidemia de la peste bubónica en las ciudades de Arequipa y Mollendo en 1903 y 1905 y el covid-19, con un enfoque en el funcionamiento del sistema de salud local y nacional, en la infraestructura sanitaria disponible para enfrentar epidemias y, finalmente, en las respuestas sociales de la población respecto al escepticismo de las medidas impuestas por las autoridades y al incremento de la automedicación. Conclusiones: la epidemia del covid-19 en el sur de Perú presenta importantes similitudes con la epidemia de peste bubónica que afectó a la región a inicios del siglo xx: desorden de responsabilidades, falta de previsión para hacer frente a enfermedades epidémicas, infraestructura precaria y una población con alto grado de desconfianza frente a las recomendaciones de las autoridades civiles y sanitarias
Introduction: The covid-19 epidemic has revealed a series of inequality problems in the access to public health services in Peru, especially in provinces far from the capital where precariousness in both infra-structure and sanitary personnel is observed. In this study, we analyzed how the population and author-ities in southern Peru faced epidemics, using covid-19 epidemic and bubonic plague in 1903 and 1905 as case studies to understand continuities in the management of epidemic crises and social response to them. Development: We studied the bubonic plague epidemic in the cities of Arequipa and Mollendo in 1903 and 1905 as well as the covid-19 epidemic, focusing on the functioning of local and national health systems, health infrastructure available to face epidemics, and finally, the social response of the population, paying particular attention to the skepticism of the population toward measures imposed by the authorities and increase in self-medication. Conclusions: The covid-19 epidemic in southern Peru pres-ents important similarities with the bubonic plague epidemic that affected the region at the beginning of the 20th century, including a disorder of responsibilities, lack of foresight to face epidemic diseases, insufficient infrastructure, and a population with a high degree of distrust in the recommendations given by the civil and health authorities
Introdução: a epidemia de covid-19 expôs uma série de problemas de desigualdade no acesso à saúde pública no Peru, especialmente nas províncias distantes da capital onde há precariedade tanto de infraestrutura quanto de pessoal de saúde. Este artigo analisa como o governo e a população têm enfren-tado epidemias no sul do Peru, utilizando o covid-19 e a peste bubônica ocorrida em 1903 e 1905 como estudos de caso, a fim de compreender as continuidades na gestão da crise epidêmica e suas respostas. Desenvolvimento: são estudadas as epidemias de peste bubônica nas cidades de Arequipa e Mollendo em 1903 e 1905 e a covid-19, enfocando no funcionamento do sistema de saúde local e nacional, a infraes-trutura de saúde disponível para enfrentar as epidemias e, por fim, as respostas da população, com particular atenção para o ceticismo em relação às medidas impostas pelas autoridades e ao aumento da automedicação. Conclusões: a epidemia de covid-19 no sul do Peru apresenta semelhanças importantes com a epidemia de peste bubônica que afetou a região no início do século XX: desordem de responsabili-dades, falta de previsão para enfrentar as doenças epidêmicas, infraestrutura precária e uma população com alto grau de desconfiança em relação às recomendações das autoridades civis e sanitárias
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Humanos , Epidemias , Perú , Peste , Infraestructura Sanitaria , Sistemas de Salud , Salud Pública , Personal de SaludRESUMEN
Background During clinical tutoring, medical students are trained to transfer theoretical knowledge to real life situations that need to be solved. Tutors should be trained to acquire skills to encourage motivation, transfer experience and inspire interest in learning among students. Aim To inquire about interests and needs during the daily work of clinical tutors. Material and Methods A qualitative study inserted in the Grounded Theory, based on the description of the meanings, in which ten clinical tutors responded to an interview in their own workplace. After obtaining consent, the responses were recorded and transcribed using Georgi's method for analysis. Results Teachers feel that there is a gap between the theoretical basis of tutorial teaching and the reality. They are especially interested in a teaching process based on values. They recognize that their pedagogical skills should be improved to improve their communication with students. They also feel that they require more skills to transfer theoretical knowledge to clinical situations. Conclusions A personal satisfaction for educating and training, the transmission of experiences, empathy and being assertive are emerging values that motivate tutors to deliver a quality education, above remunerations or contractual ties. However, the skills to transfer clinical knowledge to students should be improved.
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Medicina , EnseñanzaRESUMEN
Applying electrical stimulation (ES) could affect different cellular mechanisms, thereby producing a bactericidal effect and an increase in human cell viability. Despite its relevance, this bioelectric effect has been barely reported in percolated conductive biopolymers. In this context, electroactive polycaprolactone (PCL) scaffolds with conductive Thermally Reduced Graphene Oxide (TrGO) nanoparticles were obtained by a 3D printing method. Under direct current (DC) along the percolated scaffolds, a strong antibacterial effect was observed, which completely eradicated S. aureus on the surface of scaffolds. Notably, the same ES regime also produced a four-fold increase in the viability of human mesenchymal stem cells attached to the 3D conductive PCL/TrGO scaffold compared with the pure PCL scaffold. These results have widened the design of novel electroactive composite polymers that could both eliminate the bacteria adhered to the scaffold and increase human cell viability, which have great potential in tissue engineering applications.
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Homeopathy arrived from the United States to Peruvian soil in the last decades of the nineteenth century, broadening the repertoire of existing medical knowledge, which included an emerging medical profession, Chinese herbalists, and indigenous practitioners. This article examines the circulation and use of homeopathic therapies and medicines in Lima from the time when the American homeopath George Deacon initiated his practice, in the 1880s, until his death, in 1915. Although homeopathy was not the most widely used medical therapy in the country, it nevertheless posed a threat to professional medicine and the School of Medicine's desired monopoly of the field of medicine.
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Homeopatía/historia , Gobierno Federal/historia , Regulación Gubernamental/historia , Historia del Siglo XIX , Historia del Siglo XX , Homeopatía/legislación & jurisprudencia , Humanos , Licencia Médica/historia , Perú , Facultades de Medicina/historia , Estados UnidosRESUMEN
Abstract Homeopathy arrived from the United States to Peruvian soil in the last decades of the nineteenth century, broadening the repertoire of existing medical knowledge, which included an emerging medical profession, Chinese herbalists, and indigenous practitioners. This article examines the circulation and use of homeopathic therapies and medicines in Lima from the time when the American homeopath George Deacon initiated his practice, in the 1880s, until his death, in 1915. Although homeopathy was not the most widely used medical therapy in the country, it nevertheless posed a threat to professional medicine and the School of Medicine's desired monopoly of the field of medicine.
Resumo A homeopatia originária dos EUA adentrou solo peruano nas últimas décadas do século XIX, ampliando o repertório de conhecimento médico existente até então, o qual incluía uma profissão médica em ascensão, herbolários chineses e médicos locais. Este artigo analisa a circulação e o uso de tratamentos e medicamentos homeopáticos em Lima desde o período em que o homeopata norte-americano George Deacon iniciou sua prática, nos anos 1880, até sua morte, em 1915. Embora a homeopatia não fosse o tratamento médico mais disseminada no país, ela representou uma ameaça à medicina profissional e ao monopólio do campo da medicina almejado pela escola tradicional.
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Humanos , Historia del Siglo XIX , Historia del Siglo XX , Homeopatía/historia , Perú , Facultades de Medicina/historia , Estados Unidos , Gobierno Federal/historia , Regulación Gubernamental/historia , Homeopatía/legislación & jurisprudencia , Licencia Médica/historiaRESUMEN
Resumen Introducción: el presente artículo examina los factores que han ocasionado el precario estado de la salud mental en Perú, donde casi el 90 % de pacientes con síntomas asociados con problemas mentales no reciben tratamiento. Desarrollo: se analiza el programa neoliberal de salud implementado durante el gobierno de Alberto Fujimori (1990-2000). Posteriormente, se estudia cómo esta política pública afectó al programa de salud mental y al estado de la salud de la población. Finalmente, se examinan diversos diagnósticos sobre la salud mental posterior al retorno a la democracia en el año 2000, desde una perspectiva de los derechos humanos. Esta investigación finaliza el año 2006, momento en que el gobierno presentó un nuevo Plan Nacional de Salud Mental. Conclusiones: la falta de acceso a un tratamiento adecuado a nivel nacional ha sido consecuencia directa del reducido gasto del Estado en materia de salud, en especial hacia las enfermedades no transmisibles y de salud mental. Las políticas neoliberales de salud implementadas durante el gobierno de Alberto Fujimori redujeron el rol del Estado como garante del acceso universal a los servicios de salud, y situaron a la salud mental en un lugar marginal dentro de la salud pública. El retorno a la democracia en el año 2000, el informe final de la Comisión de la Verdad y Reconciliación (2003) y el llamado de organismos internacionales, Iglesias Católicas y Evangélicas, junto con ONG, presionaron al gobierno para situar a la salud mental como parte importante de la agenda de pública de salud.
Abstract Objective: This paper examines the elements that have caused the precarious state of mental health in Peru, where almost 90 % of patients with symptoms associated with mental disorders do not receive treatment. Development: The article analyzes the neoliberal health program implemented during the government of Alberto Fujimori (1990-2000). Subsequently, it studies how this public policy affected the mental health program and the health status of the population. Finally, it examines several diagnoses on mental health after the return of democracy in the year 2000 from a human rights perspective. This research ended in 2006 when the government presented a new National Mental Health Plan. Conclusions: The lack of access to adequate treatment at the national level has been a direct consequence of the reduced state expenditure on health, especially towards non-transmissible diseases and mental health. The neoliberal health policies implemented during the government of Alberto Fujimori reduced the role of the State as guarantor of universal access to health services and placed mental health in a marginal place within public health. The return to democracy in 2000, the final report of the Truth and Reconciliation Commission (2003), and the call of international organizations, Catholic and Evangelical Churches, together with NGOS, pressed to the government to situate mental health as a relevant part of public health agenda.
Resumo Objetivo: o presente artigo examina os fatores que têm ocasionado o precário estado da saúde mental no Peru, onde quase o 90 % de pacientes com sintomas associados com problemas mentais não recebem tratamento. Desenvolvimento: se analisa o programa neoliberal de saúde implementado durante o governo de Alberto Fujimori (1990-2000). Posteriormente estuda-se como esta política pública afetou ao programa de saúde mental e ao estado de saúde da população. Finalmente se examinam diversos diagnósticos sobre a saúde mental posterior ao retorno da democracia no ano 2000 desde uma perspectiva dos direitos humanos. Esta pesquisa finaliza no ano 2006, momento em que o governo apresentou um novo Plano Nacional de Saúde Mental. Conclusões: a falta de acesso a um tratamento adequado no nível nacional tem sido consequência direta do reduzido gasto do Estado em matéria de saúde, em especial para as doenças não transmissíveis e de saúde mental. As políticas neoliberais de saúde implementadas durante o governo de Alberto Fujimori reduziram o rol do Estado como garante do acesso universal aos serviços de saúde, e situaram à saúde mental em um lugar marginal dentro da saúde pública. O retorno à democracia no ano 2000, o informe final da Comissão da Verdade e Reconciliação (2003), e o chamado de organismos internacionais, Igrejas Católicas e Evangélicas, junto com ONGs pressionaram ao governo para situar à saúde mental como parte importante da agenda pública de saúde.
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Humanos , Salud Mental , Perú , Política Pública , Disparidades en el Estado de SaludRESUMEN
Medulloblastoma (MB) is the most common malignant brain tumor in children. Recent advances in molecular technologies allowed to classify MB in 4 major molecular subgroups: WNT, SHH, Group 3 and Group 4. In cancer research, cancer cell lines are important for examining and manipulating molecular and cellular process. However, it is important to know the characteristics of each cancer cell line prior to use, because there are some differences among them, even if they originate from the same cancer type. This study aimed to evaluate the similarities and differences among four human medulloblastoma cell lines, UW402, UW473, DAOY and ONS-76. The medulloblastoma cell lines were analyzed for (1) cell morphology, (2) immunophenotyping by flow cytometry for some specifics surface proteins, (3) expression level of adhesion molecules by RT-qPCR, (4) proliferative potential, (5) cell migration, and (6) in vivo tumorigenic potential. It was observed a relationship between cell growth and CDH1 (E-chaderin) adhesion molecule expression and all MB cell lines showed higher levels of CDH2 (N-chaderin) when compared to other adhesion molecule. ONS-76 showed higher gene expression of CDH5 (VE-chaderin) and higher percentage of CD144/VE-chaderin positive cells when compared to other MB cell lines. All MB cell lines showed low percentage of CD34, CD45, CD31, CD133 positive cells and high percentage of CD44, CD105, CD106 and CD29 positive cells. The DAOY cell line showed the highest migration potential, the ONS-76 cell line showed the highest proliferative potential and only DAOY and ONS-76 cell lines showed tumorigenic potential in vivo. MB cell lines showed functional and molecular differences among them, which it should be considered by the researchers in choosing the most suitable cellular model according to the study proposal.
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Several studies have tried to associate the presence of different pathogens with the onset and progression ofperiodontitis, reporting a wide variety of results from different populations and environments. The aim of this study was to determine the main periodontal pathogens present in the subgingival biofilm of Dominican patients with periodontitis, by using specific microbiological culturing techniques. Periodontitis patients were selected after a full-mouth periodontal evaluation, and assigned to different periodontitis groups based on percentage of affected locations. Subgingival samples were collected and analyzed by means of specific culture techniques. Anaerobic counts, frequency of detection and proportions of target pathogens were calculated. Variables were analyzed by means of Student's T-test or chi-square test. Twenty-nine subjects were recruited, of whom 17 were diagnosed with generalized periodontitis (GenP) and 12 with localized periodontitis (LocP). The most prevalent bacterial species in both groups was Prevotella intermedia (94.1% in GenP and 91.7% in LocP), followed by Porphyromonas gingivalis (88.2% in GenP and 83.3% in LocP). Total microbiota in subgingival samples was 1.3 x107 colony-forming units (CFU)/mL (standard deviation, SD=1.5 x107) and 9.6x10s CFU/mL (SD=1.1 x107) in GenP and LocP subjects, respectively, though differences were not statistically significant (p=0.222). The highest counts were observed for P gingivalis in both groups, with mean concentration 2.5x10s CFU/mL (6.1x10s) in GenP and 2.9x10s CFU/mL (5x10s) in LocP, with no statistically significant difference (p=0.879). These results suggest that relevant periodontal pathogens are found with diversity and abundance in the subgingival microbiota of adult Dominican patients with periodontitis.
Varios estudios han tratado de asociar la presencia de diferentes patógenos con el inicio y la progresión de la periodontitis, mostrando una gran variedad de resultados en diferentes poblaciones y entornos. El objetivo del presente estudio fue determinar los principales patógenos periodontales presentes en la biopelícula subgingival de pacientes dominicanos con periodontitis, utilizando técnicas específicas de cultivo microbiológico. Los pacientes con periodontitis se seleccionaron después de una evaluación periodontal de boca completa y se asignaron a diferentes grupos de periodontitis según el porcentaje de localizaciones afectadas. Las muestras subgingivales fueron recolectadas y analizadas mediante técnicas de cultivo específicas. Se calcularon los recuentos anaeróbicos, la frecuencia de detección y las proporciones de los patógenos seleccionados. Las variables se analizaron mediante la prueba T de Student o la prueba de chi-cuadrado. Se reclutaron veintinueve sujetos, 17 diagnosticados como periodontitis generalizada (GenP) 12 con periodontitis localizada (LocP). La especie bacteriana más prevalente en ambos grupos fue Prevotella intermedia (94.1% y 91.7%, respectivamente) y seguida de Porphyromonas gingivalis (88.2% y 83.3%, respectivamente). La microbiota total en muestras subgingivales fue 1.3 x107 unidades formadoras de colonias (CFU)/mL (desviación estándar, SD=1.5 x107) y 9.6x106 CFU / mL (SD=1.1 x107) en sujetos GenP y LocP, respectivamente, pero no hubo diferencias estadísticamente significativas (p=0.222). Los recuentos más altos se observaron para P. gingivalis en ambos grupos, con una concentración media de 2.5x106 CFU/mL (6.1x106) en GenP y 2.9x106 CFU/mL (5x106) en LocP, sin diferencias estadísticamente significativas (p=0.879). Estos resultados sugieren que se encuentran patógenos periodontales relevantes con diversidad y abundancia en la microbiota subgingival de pacientes adultos dominicanos con periodontitis.