RESUMEN
A cell line called HCxPy was obtained in vitro by transformation of dissociated hamster brain cell cultures by polyoma virus. The first foci of transformed cells became evident 90 to 120 days after viral infection. This cell line is now at the 46th passage. The cells appear tumorigenic for hamsters after subcutaneous and intracerebral injection. They carry the polyoma virus T and cell surface antigens. Good evidence for astrocytic differentiation can be found by morphological examination of the tumours and of the cultured cells.
Asunto(s)
Transformación Celular Neoplásica , Corteza Cerebral/microbiología , Poliomavirus , Animales , Antígenos de Neoplasias/análisis , Antígenos Virales/análisis , Transformación Celular Neoplásica/patología , Células Cultivadas , Cricetinae , Poliomavirus/inmunología , Factores de TiempoRESUMEN
The authors report premilinary results of an experiment on permeability of the blood-brain barrier (BBB) to anti-tumor virus-induced immunological factors in the polyoma virus/Syrian Hamster system. The animals were protected by subcutaneous or intracranial injections with virus before challenge with polyoma virus transformed cells by both routes. BBB seemed to be permeable to the efferent part of the subcutaneously induced immune reaction. On the contrary, antigenic information introduced in the central nervous system was trapped inside the BBB. Thus the BBB might offer a "one-way" permeability in this system.
Asunto(s)
Barrera Hematoencefálica , Poliomavirus/inmunología , Sarcoma Experimental/inmunología , Animales , Transformación Celular Neoplásica , Cricetinae , Trasplante de Neoplasias , Sarcoma Experimental/patologíaRESUMEN
The authors report two personal cases of Reye's syndrome, one of which was studied by electron microscopy, and comment on the pathological characteristics of this disease. The hepatic lesions are most important and resemble fatty degeneration rather than simple steatosis. This notion is confirmed by data obtained by electron microscopy which revealed marked abnormalities of the cellular organites, in particular, mitochondria. The nervous involvement included mainly, oedema of the nerve sheath. These facts emphasize the importance of metabolic disturbances in the physiopathology of the disease.