RESUMEN
CONTEXT: The Orion Molecular Cloud is the nearest massive-star forming region. Massive stars have profound effects on their environment due to their strong radiation fields and stellar winds. Stellar feedback is one of the most crucial cosmological parameters that determine the properties and evolution of the interstellar medium in galaxies. AIMS: We aim to understand the role that feedback by stellar winds and radiation play in the evolution of the interstellar medium. Velocity-resolved observations of the [C II] 158µm fine-structure line allow us to study the kinematics of UV-illuminated gas. Here, we present a square-degree-sized map of [C II] emission from the Orion Nebula complex at a spatial resolution of 16â³ and high spectral resolution of 0.2kms-1, covering the entire Orion Nebula (M42) plus M43 and the nebulae NGC 1973, 1975, and 1977 to the north. We compare the stellar characteristics of these three regions with the kinematics of the expanding bubbles surrounding them. METHODS: We use [C II] 158µm line observations over an area of 1.2deg2 in the Orion Nebula complex obtained by the upGREAT instrument onboard SOFIA. RESULTS: The bubble blown by the O7V star θ 1 Ori C in the Orion Nebula expands rapidly, at 13kms-1. Simple analytical models reproduce the characteristics of the hot interior gas and the neutral shell of this wind-blown bubble and give us an estimate of the expansion time of 0.2 Myr. M43 with the B0.5V star NU Ori also exhibits an expanding bubble structure, with an expansion velocity of 6kms-1. Comparison with analytical models for the pressure-driven expansion of H II regions gives an age estimate of 0.02 Myr. The bubble surrounding NGC 1973, 1975, and 1977 with the central B1V star 42 Orionis expands at 1.5kms-1, likely due to the over-pressurized ionized gas as in the case of M43. We derive an age of 0.4 Myr for this structure. CONCLUSIONS: We conclude that the bubble of the Orion Nebula is driven by the mechanical energy input by the strong stellar wind from θ 1 Ori C, while the bubbles associated with M43 and NGC 1977 are caused by the thermal expansion of the gas ionized by their central later-type massive stars.
RESUMEN
Massive stars inject mechanical and radiative energy into the surrounding environment, which stirs it up, heats the gas, produces cloud and intercloud phases in the interstellar medium, and disrupts molecular clouds (the birth sites of new stars1,2). Stellar winds, supernova explosions and ionization by ultraviolet photons control the lifetimes of molecular clouds3-7. Theoretical studies predict that momentum injection by radiation should dominate that by stellar winds8, but this has been difficult to assess observationally. Velocity-resolved large-scale images in the fine-structure line of ionized carbon ([C II]) provide an observational diagnostic for the radiative energy input and the dynamics of the interstellar medium around massive stars. Here we report observations of a one-square-degree region (about 7 parsecs in diameter) of Orion molecular core 1-the region nearest to Earth that exhibits massive-star formation-at a resolution of 16 arcseconds (0.03 parsecs) in the [C II] line at 1.9 terahertz (158 micrometres). The results reveal that the stellar wind originating from the massive star θ1 Orionis C has swept up the surrounding material to create a 'bubble' roughly four parsecs in diameter with a 2,600-solar-mass shell, which is expanding at 13 kilometres per second. This finding demonstrates that the mechanical energy from the stellar wind is converted very efficiently into kinetic energy of the shell and causes more disruption of the Orion molecular core 1 than do photo-ionization and evaporation or future supernova explosions.
RESUMEN
CONTEXT: L1630 in the Orion B molecular cloud, which includes the iconic Horsehead Nebula, illuminated by the star system σ Ori, is an example of a photodissociation region (PDR). In PDRs, stellar radiation impinges on the surface of dense material, often a molecular cloud, thereby inducing a complex network of chemical reactions and physical processes. AIMS: Observations toward L1630 allow us to study the interplay between stellar radiation and a molecular cloud under relatively benign conditions, that is, intermediate densities and an intermediate UV radiation field. Contrary to the well-studied Orion Molecular Cloud 1 (OMC1), which hosts much harsher conditions, L1630 has little star formation. Our goal is to relate the [Cii] fine-structure line emission to the physical conditions predominant in L1630 and compare it to studies of OMC1. METHODS: The [Cii] 158 µm line emission of L1630 around the Horsehead Nebula, an area of 12' × 17', was observed using the upgraded German Receiver for Astronomy at Terahertz Frequencies (upGREAT) onboard the Stratospheric Observatory for Infrared Astronomy (SOFIA). RESULTS: Of the [Cii] emission from the mapped area 95%, 13 Lâ, originates from the molecular cloud; the adjacent Hii region contributes only 5%, that is, 1 Lâ. From comparison with other data (CO(1-0)-line emission, far-infrared (FIR) continuum studies, emission from polycyclic aromatic hydrocarbons (PAHs)), we infer a gas density of the molecular cloud of nH â¼ 3 · 103 cm-3, with surface layers, including the Horsehead Nebula, having a density of up to nH â¼ 4 · 104 cm-3. The temperature of the surface gas is T â¼ 100 K. The average [Cii] cooling efficiency within the molecular cloud is 1.3 · 10-2. The fraction of the mass of the molecular cloud within the studied area that is traced by [Cii] is only 8%. Our PDR models are able to reproduce the FIR-[Cii] correlations and also the CO(1-0)-[Cii] correlations. Finally, we compare our results on the heating efficiency of the gas with theoretical studies of photoelectric heating by PAHs, clusters of PAHs, and very small grains, and find the heating efficiency to be lower than theoretically predicted, a continuation of the trend set by other observations. CONCLUSIONS: In L1630 only a small fraction of the gas mass is traced by [Cii]. Most of the [Cii] emission in the mapped area stems from PDR surfaces. The layered edge-on structure of the molecular cloud and limitations in spatial resolution put constraints on our ability to relate different tracers to each other and to the physical conditions. From our study, we conclude that the relation between [Cii] emission and physical conditions is likely to be more complicated than often assumed. The theoretical heating efficiency is higher than the one we calculate from the observed [Cii] emission in the L1630 molecular cloud.
RESUMEN
Acute myeloid leukemia (AML) is associated with poor clinical outcome and the development of more effective therapies is urgently needed. G protein-coupled receptors (GPCRs) represent attractive therapeutic targets, accounting for approximately 30% of all targets of marketed drugs. Using next-generation sequencing, we studied the expression of 772 GPCRs in 148 genetically diverse AML specimens, normal blood and bone marrow cell populations as well as cord blood-derived CD34-positive cells. Among these receptors, 30 are overexpressed and 19 are downregulated in AML samples compared with normal CD34-positive cells. Upregulated GPCRs are enriched in chemokine (CCR1, CXCR4, CCR2, CX3CR1, CCR7 and CCRL2), adhesion (CD97, EMR1, EMR2 and GPR114) and purine (including P2RY2 and P2RY13) receptor subfamilies. The downregulated receptors include adhesion GPCRs, such as LPHN1, GPR125, GPR56, CELSR3 and GPR126, protease-activated receptors (F2R and F2RL1) and the Frizzled family receptors SMO and FZD6. Interestingly, specific deregulation was observed in genetically distinct subgroups of AML, thereby identifying different potential therapeutic targets in these frequent AML subgroups.
Asunto(s)
Secuenciación de Nucleótidos de Alto Rendimiento , Leucemia Mieloide Aguda/genética , Proteínas de Neoplasias/genética , Receptores Acoplados a Proteínas G/genética , Transcriptoma/genética , Adulto , Anciano , Anciano de 80 o más Años , Antígenos CD34/genética , Células de la Médula Ósea/metabolismo , Células de la Médula Ósea/patología , Femenino , Regulación Neoplásica de la Expresión Génica , Humanos , Leucemia Mieloide Aguda/sangre , Leucemia Mieloide Aguda/patología , Masculino , Persona de Mediana Edad , Terapia Molecular Dirigida , Proteínas de Neoplasias/sangre , Transducción de Señal/genéticaAsunto(s)
Aorta Torácica/anomalías , Ruidos Respiratorios/etiología , Malformaciones Vasculares/complicaciones , Malformaciones Vasculares/diagnóstico , Aorta Torácica/diagnóstico por imagen , Aorta Torácica/patología , Humanos , Recién Nacido , Angiografía por Resonancia Magnética , Radiografía , UltrasonografíaAsunto(s)
Linfocitos T CD4-Positivos/metabolismo , Linfocitos T CD8-positivos/metabolismo , Enfermedad Injerto contra Huésped/etiología , Factor Estimulante de Colonias de Granulocitos/farmacología , Movilización de Célula Madre Hematopoyética , Leucaféresis , Trasplante de Células Madre de Sangre Periférica , Receptor Toll-Like 2/genética , Receptor Toll-Like 4/genética , Adolescente , Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Trasplante de Células Madre de Sangre Periférica/efectos adversos , Trasplante de Células Madre de Sangre Periférica/mortalidad , Polimorfismo de Nucleótido SimpleRESUMEN
Botulinum toxin is used to induce transient graded paresis by chemodenervation in the treatment of focal hyperkinetic movement disorders. While the molecular events occurring in motoneurons after mechanical nerve lesioning leading to muscle paresis are well known, they have been investigated to a lesser extent after chemodenervation. We therefore examined the expression of enkephalin (ENK), acidic fibroblast growth factor (aFGF), neurotensin (NT), galanin (GAL), substance P (SP), vasoactive intestinal polypeptide (VIP), and neuropeptide Y (NPY) in rat spinal motoneurons after chemodenervation of the gastrocnemius. In order to precisely localize the motoneurons targeting the injection site, retrograde tracing was performed in additional rats by using Fluorogold injections. ENK expression was upregulated in the region corresponding to the Fluorogold positive motoneurons, but also on the contralateral side and in more distant parts of the spinal cord. The highest upregulation occurred 7 to 14 days after injections and decreased over a period of three months. At 8 days, aFGF was slightly downregulated in all regions studied, single motoneurons showed NT expression, while expression of GAL, SP, VIP, and NPY could be detected neither in controls nor in toxin-treated animals. These alterations in gene expression were strikingly different from those described after axotomy. Our present findings give additional demonstration of the considerable plasticity of the adult spinal cord after botulinum toxin treatment.
Asunto(s)
Encefalinas/genética , Factor 1 de Crecimiento de Fibroblastos/genética , Neuronas Motoras/metabolismo , Médula Espinal/citología , Estilbamidinas , Factores de Edad , Animales , Toxinas Botulínicas , Recuento de Células , Cuerpo Estriado/química , Cuerpo Estriado/citología , Cuerpo Estriado/metabolismo , Encefalinas/análisis , Femenino , Factor 1 de Crecimiento de Fibroblastos/análisis , Colorantes Fluorescentes , Galanina/análisis , Galanina/genética , Expresión Génica/fisiología , Neuronas Motoras/química , Desnervación Muscular , Músculo Esquelético/inervación , Degeneración Nerviosa/inducido químicamente , Degeneración Nerviosa/metabolismo , Neuropéptido Y/análisis , Neuropéptido Y/genética , Neurotensina/análisis , Neurotensina/genética , ARN Mensajero/análisis , Ratas , Ratas Wistar , Médula Espinal/química , Médula Espinal/metabolismo , Sustancia P/análisis , Sustancia P/genética , Péptido Intestinal Vasoactivo/análisis , Péptido Intestinal Vasoactivo/genéticaAsunto(s)
Colitis Ulcerosa/diagnóstico por imagen , Colon/diagnóstico por imagen , Leucocitos , Megacolon Tóxico/diagnóstico por imagen , Adulto , Colon/patología , Dilatación Patológica , Granulocitos , Humanos , Radioisótopos de Indio , Masculino , Compuestos Organometálicos , Oxiquinolina/análogos & derivados , CintigrafíaRESUMEN
In a 55 year old man with renal insufficiency requiring hemodialysis after bilateral nephrectomy who had been hospitalized with febrile illness and enteritis, Campylobacter fetus subspecies fetus could be isolated from the blood and feces. Antibodies against the homologous strain (isolated from the blood) could be detected in the serum by means of the Widal test and the indirect immunofluorescence test. Culturing of Campylobacter fetus subspecies fetus (especially from blood cultures) may be difficult because of the poor growth conditions for microaerophilic bacteria. It is quite possible that this organism occurs more frequently in blood and feces than suggested by the routine investigations carried out so far.