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Eur J Biochem ; 270(1): 20-7, 2003 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-12492471

RESUMEN

Strains of Pseudomonas syringae pv. porri are characterized by a number of pathovar-specific phenotypic and genomic characters and constitute a highly homogeneous group. Using monoclonal antibodies, they all were classified in a novel P. syringae serogroup O9. The O polysaccharides (OPS) isolated from the lipopolysaccharides (LPS) of P. syringae pv. porri NCPPB 3365 and NCPPB 3364T possess multiple oligosaccharide O repeats, some of which are linear and composed of l-rhamnose (l-Rha), whereas the major O repeats are branched with l-rhamnose in the main chain and GlcNAc in side chains (structures 1 and 2). Both branched O repeats, which differ in the position of substitution of one of the Rha residues and in the site of attachment of GlcNAc, were found in the two strains studied, O repeat 1 being major in strain NCPPB 3365 and 2 in strain NCPPB 3364T. [formula: see text]. The relationship between OPS chemotype and serotype on one hand and the genomic characters of P. syringae pv. porri and other pathovars delineated in genomospecies 4 on the other hand is discussed.


Asunto(s)
Antígenos O/química , Pseudomonas/química , Pseudomonas/clasificación , Conformación de Carbohidratos , Secuencia de Carbohidratos , Genoma Bacteriano , Espectroscopía de Resonancia Magnética , Datos de Secuencia Molecular , Pseudomonas/genética , Ramnosa/química , Serotipificación/métodos
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