RESUMEN
Electron-microscopic study of the interaction of Y. pestis and peritoneal phagocytes obtained from BALB mice, both intact and treated with the toxic fraction of Y. pestis, has revealed that in the process of phagocytosis the toxin weakens the killing and degradation phases of these bacteria, but leaves the ingestion activity of phagocytes unchanged. The process of phagocytosis in mice exposed to toxin is accompanied by destructive changes of cells and redistribution of myeloperoxidase, a bactericidal enzyme, in polymorphonuclear leukocytes without any signs of bacterial degradation.
Asunto(s)
Toxinas Bacterianas/farmacología , Fagocitosis/efectos de los fármacos , Yersinia pestis , Animales , Macrófagos Peritoneales/efectos de los fármacos , Macrófagos Peritoneales/ultraestructura , Ratones , Ratones Endogámicos BALB C , Microscopía Electrónica , Neutrófilos/efectos de los fármacos , Neutrófilos/ultraestructura , Yersinia pestis/ultraestructuraRESUMEN
Significant data on the dynamics of globulin production in guinea pigs in the process of immunogenesis after the injection of Francisella tularensis vaccine strain or conjugated brucellosis vaccine have been obtained by means of immunofluorescence and the enzyme immunoassay. The number of globulin-producing cells in lymphoid organs (the spleen, regional and remote lymph nodes) differs, depending on the injected antigen. The relationship between the character of immunomorphological changes in lymphoid organs and the dynamics of the increase of antibody titers in the peripheral blood of the animals after their immunization with conjugated brucellosis vaccine and the injection of avirulent F. tularensis has been established.
Asunto(s)
Vacunas Bacterianas/inmunología , Vacuna contra la Brucelosis/inmunología , Francisella tularensis/inmunología , Sistema Inmunológico/citología , Inmunoglobulinas/biosíntesis , Animales , Anticuerpos Antibacterianos/sangre , Formación de Anticuerpos/inmunología , Células Productoras de Anticuerpos/citología , Células Productoras de Anticuerpos/inmunología , Cobayas , Sistema Inmunológico/inmunología , Inmunoglobulinas/análisis , Factores de TiempoRESUMEN
Anthrax toxin introduced subcutaneously into white rats Fisher-344 caused specific intoxication accompanied by characteristic pathomorphological changes and, when studied by the immunoperoxidase method in histological sections, could be detected in the blood stream (in plasma) and in the cytoplasm of macrophages (in the lungs and the spleen). The content of the preparation introduced into the animals in the blood stream is directly related to its toxicity, being probably indicative of the relative functional failure of the macrophagal system.
Asunto(s)
Bacillus anthracis , Toxinas Bacterianas/toxicidad , Animales , Carbunco/etiología , Carbunco/inmunología , Carbunco/patología , Antígenos Bacterianos/análisis , Bacillus anthracis/inmunología , Toxinas Bacterianas/análisis , Epítopos/análisis , Técnicas para Inmunoenzimas , Macrófagos/inmunología , Ratas , Ratas Endogámicas F344RESUMEN
The possibility of evaluating the immunogenic potency of brucellosis vaccine BA-19 by immunological methods has been shown. Morphometry and the quantitative evaluation of globulin-producing cells in lymphoid organs by direct and indirect immunofluorescent techniques serve as informative evaluation tests. The marker method with the evaluation of lymphocyte classes by acid and alkaline phosphatases and the electrophoretic motility characteristics of T- and B-lymphocytes, used in combination with the above-mentioned methods, present information on immunogenesis. The immunoperoxidase method and the values of the opsonophagocytic index characterize the state of phagocytosis and the persistence of the antigen.
Asunto(s)
Vacuna contra la Brucelosis/inmunología , Animales , Linfocitos B/citología , Linfocitos B/inmunología , Vacuna contra la Brucelosis/administración & dosificación , Brucella abortus/inmunología , Citofotometría , Evaluación Preclínica de Medicamentos , Cobayas , Inmunoglobulinas/biosíntesis , Ganglios Linfáticos/anatomía & histología , Ganglios Linfáticos/inmunología , Fagocitosis , Bazo/anatomía & histología , Bazo/inmunología , Linfocitos T/citología , Linfocitos T/inmunología , Factores de TiempoRESUMEN
The possibility of evaluating functional immunomorphogenesis in the course of the vaccinal process after the injection of conjugated and live brucellosis vaccines, as well as conjugated plague antigen and Yersinia pestis strain EV, to guinea pigs has been shown by means of the direct and two-layer variants of the immunoperoxidase assay. The dynamics of the accumulation of globulin-producing cells in the immunocompetent organs and the time course of immunoglobulin titers in the peripheral blood after the injection of live and conjugated vaccines have been followed up. These data may be used for the morphological evaluation of approved preparations.