RESUMEN
5-Fluorouracil (5-FU)-based chemotherapies with irinotecan have been applied for the treatment of cancers, and a common dose-limiting toxicity is neutropenia and diarrhea. In this study, we investigated the effect of 5-FU treatment on expression levels of drug transporters for SN-38 transportation and SN-38 absorption from the intestine following 5-FU treatment. Expression levels of several drug transporters and nuclear receptors in rats after 5-FU treatment were evaluated. SN-38 absorption from the intestine was evaluated by SN-38 concentration levels in serum following SN-38 injection into the intestine of 5-FU treated rats. The levels of renal multidrug resistance protein 2 (Mrp2) on day 4 after treatment (400 mg/kg) showed significant upregulation, 359.2 ± 33.2% (mean ± S.E.) of control. Mrp2 levels in the intestine were downregulated to 26.2 ± 8.4% of control. 5-FU treatment (400 mg/kg) also significantly downregurated expression levels of P-glycoprotein (P-gp) and breast cancer resistance protein (Bcrp) to 41.2 ± 14.7%, 15.7 ± 4.3% of control, respectively. To evaluate SN-38 absorption from the intestine, SN-38 was loaded in to the intestine on day 4 after 5-FU treatment. Pretreatment with 5-FU significantly increased SN-38 concentration in the blood 30, 60 and 90 min after SN-38 administration. The area under the curve for SN-38 in the 5-FU group was significantly higher than in vehicle groups. 5-FU treatment decreased expression levels of P-glycoprotein and Bcrp in intestine. The present study suggests that combination chemotherapy of 5-FU with irinotecan (CPT-11) may elevate SN-38 absorption from intestine.
Asunto(s)
Camptotecina/análogos & derivados , Fluorouracilo/farmacología , Absorción Intestinal/efectos de los fármacos , Transportadoras de Casetes de Unión a ATP/genética , Animales , Secuencia de Bases , Peso Corporal/efectos de los fármacos , Camptotecina/farmacocinética , Línea Celular , Cartilla de ADN , Humanos , Irinotecán , Masculino , Fosforilación , Reacción en Cadena de la Polimerasa , Proteínas Proto-Oncogénicas c-jun/metabolismo , ARN Mensajero/genética , Ratas , Ratas Wistar , Receptores Citoplasmáticos y Nucleares/genéticaRESUMEN
PURPOSE: To determine effects of light curing of self-etching adhesives under ambient air or nitrogen on inhibition depths, enamel and dentin bond strengths, and marginal adaptation in dentin cavities. MATERIALS AND METHODS: The adhesives investigated were: AQ Bond (AQB, Sun Medical; Kyoto, Japan), iBond (IBO, Heraeus Kulzer; Hanau, Germany), One-Up Bond F II (OUB, Tokuyama; Tokyo, Japan), and Prompt L-Pop (PLP, 3M/ESPE; Seefeld, Germany). Inhibition layer thickness (ILT) was microscopically measured on 3 disk-shaped specimens each. Shear bond strengths (SBS) on enamel and dentin (n = 8) were determined after 10 min and 24 h water storage of bonded composite specimens (Venus, Heraeus Kulzer). The marginal adaptation of bonded Venus restorations in cylindrical dentin cavities was microscopically evaluated after 10 min of specimen storage (n = 8) in water. The data were statistically analyzed using parametric and nonparametric ANOVA and post-hoc tests at p < 0.05. RESULTS: Adhesive curing under air revealed significant ILT (microm) differences: IBO (4) < AQB (8) < PLP (12) < OUB (23). Upon curing in nitrogen atmosphere, no inhibition occurred with AQB, IBO, and PLP, and < 3 microm for OUB. SBSs on enamel after 10 min or 24 h did not differ according to curing atmospheres (p > 0.05). On dentin, SBSs were higher after curing under N2 (p < 0.05). Irrespective of the curing atmosphere, marginal adaptation was good for AQB and IBO and poor for PLP; OUB revealed significantly smaller gaps under N2 than under air curing. CONCLUSION: Oxygen inhibition of the four self-etching adhesives investigated had no or only moderate effects on bonding efficacy to enamel and dentin.
Asunto(s)
Recubrimiento Dental Adhesivo , Esmalte Dental/ultraestructura , Recubrimientos Dentinarios/química , Dentina/ultraestructura , Oxígeno/química , Aire , Resinas Compuestas/química , Adaptación Marginal Dental , Humanos , Ensayo de Materiales , Metacrilatos/química , Nitrógeno/química , Polímeros/química , Cementos de Resina/química , Resistencia al Corte , Estrés Mecánico , Propiedades de Superficie , Factores de Tiempo , Agua/químicaRESUMEN
OBJECTIVE: The aim was to investigate whether anion exchange resin powder in the OH(-) form, added to the amine component of self-cured resin can deprotonize acid groups of a self-etching adhesive and thus eliminate incompatibility caused by amine neutralization. METHODS: Shear bond strength (SBS) of a self-cured CorePaste (COP), bonded either with all-in-one adhesive iBond (IBO) or with IBO coated with a self-cured experimental resin (SCR), loaded with 0, 8, 16, or 32 wt% of anion exchange resin powder (AER) in OH- form, were determined on 80 enamel and dentin surfaces each after 24 h water storage. IBO specimens were either light-activated or not. Flexural modulus and strength of the four SCR resins were determined by three-point bending. Data was analyzed by one-way ANOVA and Duncan's post hoc test (p<0.05). RESULTS: Enamel SBSs of COP on IBO were 20-25 MPa, in combination with SCR (0 up to 32%) >25 MPa for activated or non-activated IBO. COP on IBO produced no bond on dentin, with the non-loaded SCR 5-10 MPa SBS was achieved, and 15 and 21 MPa were measured with the three AER-loaded SCRs on activated and non-activated IBO, respectively. Flexural moduli of SCR with 0 and 8% AER were higher than with 16 and 32%. Flexural strengths were not significantly different. SIGNIFICANCE: Addition of anion exchange resin to the amine component of self-cured resin is an effective means of enhancing the bond strength on dentin and to prevent amine neutralization through the acid groups of self-etching primer adhesives.
Asunto(s)
Resinas Compuestas/química , Recubrimiento Dental Adhesivo , Resinas de Intercambio Iónico , Cementos de Resina/química , Grabado Ácido Dental , Aminas/química , Análisis de Varianza , Aniones , Análisis del Estrés Dental , Incompatibilidad de Medicamentos , Elasticidad , Ensayo de Materiales , Oxidación-Reducción , Transición de Fase , Docilidad , Resistencia al Corte , Estadísticas no ParamétricasRESUMEN
OBJECTIVE: To investigate the resistance to cyclic fatigue of maxillary incisors with flared canals restored with different post-and-core materials. METHODS: Thirty human maxillary central incisors were assigned randomly to two main groups (non-ferrule and 1-mm ferrule) of 15. Each main group was then divided into 3 groups of 5 specimens and restored with custom cast post-and-core (MPC), resin composite post-and-core (RCP), and resin composite core combined with prefabricated carbon fiber post (FRC), respectively. Every group was subjected to a cyclic fatigue test, and recorded the numbers of load cycles that occurred tooth fracture. All data were analyzed with two-way ANOVA and the Tukey HSD test at alpha = 0.05. RESULTS: FRC revealed significantly higher fatigue strength than the other groups (P < 0.05). Preparing a dentin ferrule increased significantly fatigue resistance (P < 0.05). The favorable (retrieval) fracture pattern of the tested specimens was discovered only in RCP. CONCLUSIONS: Using FRC may get a long fatigue life in restoring pulpless teeth with flared canals. Dentin ferrule preparation is necessary to enhance resistance of the restorations to cyclic fatigue.
Asunto(s)
Carbono , Resinas Compuestas , Cavidad Pulpar/patología , Técnica de Perno Muñón , Diente no Vital/terapia , Fibra de Carbono , Aleaciones de Cromo , Materiales Dentales , Humanos , Ensayo de MaterialesRESUMEN
The aim of this study was to evaluate the resistance to fracture of endodontically treated teeth with flared canals restored with different post and core restorations under static and cyclic fatigue loadings. Sixty human maxillary central incisors were used. Two main groups (non-ferrule and 1-mm ferrule) were divided into three types of restoration: custom cast post-and-core (MPC), resin composite post-and-core (RCP), and resin composite core in combination with prefabricated carbon fiber post (FRC). Half of each group was subjected to a static loading test, and the other to a cyclic fatigue test. FRC exhibited a significantly higher number of load cycles than the other groups, and MPC showed the highest failure load among the tested groups. However, all FRC and MPC specimens demonstrated unfavorable root fractures. The results of this study suggested that RCP prepared with 1-mm ferrule was the most desirable restoration for structurally compromised roots, as relatively strong resistance to cyclic fatigue and fracture was revealed--given that all RCP specimens demonstrated favorable root fracture.
Asunto(s)
Fracaso de la Restauración Dental , Técnica de Perno Muñón , Fracturas de los Dientes/prevención & control , Diente no Vital , Análisis de Varianza , Carbono , Fibra de Carbono , Resinas Compuestas , Fuerza Compresiva , Diseño de Prótesis Dental , Análisis del Estrés Dental , Aleaciones de Oro , Humanos , Incisivo , Preparación del Conducto Radicular/métodos , Fracturas de los Dientes/etiología , Diente no Vital/complicacionesRESUMEN
PURPOSE: The purposes of this study were to evaluate the shear bond strength of luting glass-ionomer cement to a dentin surface treated by pulsed Nd:YAG laser irradiation, and to prove the hypothesis that the bond strength of glass-ionomer luting cement to dentin is favorably altered after Nd:YAG laser irradiation. MATERIALS AND METHODS: Sixty-four extracted human molars with an exposed flat dentin surface were divided into four groups (n = 16). After painting black ink on their surfaces, the teeth of groups 1, 2, and 3 were irradiated by an Nd:YAG laser at 1.064-microm wavelength at 1, 2, and 3 W, respectively; group 4 was untreated and served as a control. Some specimens of each group were used for morphologic and atomic analytic study, and the others were used for shear bond testing. The shear bond test was performed after cylindric titanium specimens were cemented with glass-ionomer cement on a circular dentin area and specimens were immersed in distilled water. The tested specimens were also morphologically investigated. RESULTS: Shear bond strength of group 3 was significantly higher than that of the control group. Morphologic observation of the cement-dentin interface showed good adaptation of the luting cement to laser-treated dentin. Analysis of atomic contents on the dentin surface showed a significant increase of calcium:phosphorus ratio after laser irradiation. CONCLUSION: The dentin surface was modified morphologically and chemically, and the shear bond strength of glass-ionomer luting cement to dentin was increased by Nd:YAG laser irradiation at 3 W.