RESUMEN
Carbol fuchsin (Ziehl-Neelsen) staining is a major method used since the late nineteenth century to detect Mycobacterium tuberculosis in the tissues. At the same time, the number of mycobacteria does not frequently show a clinical and morphological correspondence to the activity of the infectious process. OBJECTIVE: To reveal the morphological characteristics of Mycobacterium tuberculosis in patients with long-lasting tuberculosis. MATERIAL AND METHODS: Twenty-four autopsies of verified fibrous-cavernous tuberculosis were examined. Paraffin sections were stained with hematoxylin and eosin, carbol fuchsin (Ziehl-Neelsen), or auramine-rhodamine (followed by luminescence microscopic examination); an immunohistochemical (IHC) study with serum to PAV (Protein antigen B) was conducted. RESULTS AND DISCUSSION: The changes characteristic of progressive tuberculosis were revealed in all the cases. The Ziehl-Neelsen staining revealed isolated clusters of acid-fast bacilli (a total of 1,000 in less than 10 fields of view). When stained with auramine-rhodamine, the number of found mycobacteria proved to be significantly larger (a total of 1,000 to 10,000 in 10-50 fields of view) and that was greatest in the IHC study (a total of over 10,000 in more than 50 fields of view). At the same type, all types of studies revealed that the localization of mycobacteria was exclusively extracellular. The Ziehl-Neelsen staining indicated that the proportion of typical bacilli was much higher (85-95%; mean 88.13±2.14) than that (50-85%; mean 64.38±4.24%) identified when stained with auramine-rhodamine and even more significantly more than that (50-70%; mean 57.29±2.78%) detected during the IHC study. The indicators were equally different for the atypical morphological forms of mycobacteria identified in minimal quantities by the Ziehl-Nielsen staining and in large quantities by fluorescence and IHC studies. CONCLUSION: There is evidence for the ability of mycobacteria to have morphological polymorphism and for the need to clarify the pathogenesis of tuberculosis.
Asunto(s)
Mycobacterium tuberculosis , Tuberculosis , Colorantes , Humanos , Mycobacterium tuberculosis/aislamiento & purificación , Coloración y Etiquetado , Tuberculosis/diagnóstico , Tuberculosis/microbiologíaRESUMEN
The paper describes the clinical, morphological, and immunohistochemical characteristics of 13 granular cell tumors of the upper airway. These tumors are shown to have virtually the same histological and immunohistochemical features as granular cell tumors at another site. The histogenesis of these tumors is discussed. There are currently a number of more or less solid grounds for considering them as neurogenic tumors to be close to schwannomas. At the same time one cannot ignore the fact that there is morphological and immunohistochemical evidence for that the granular cell tumors have rather cytotypical than histotypical properties, which cannot implicitly assign them to nerve tissue tumors. Most likely, the granular cell tumors belong to a histogenetically heterogeneous mixed group, in this connection their place in the classification of tumors needs further investigation, by applying the criteria developed by Russian histologists and oncomorphologists.
Asunto(s)
Tumor de Células Granulares/patología , Tumor Mixto Maligno/patología , Neoplasias del Sistema Respiratorio/patología , Adolescente , Adulto , Niño , Tumor de Células Granulares/clasificación , Humanos , Masculino , Persona de Mediana Edad , Tumor Mixto Maligno/clasificación , Neurilemoma/clasificación , Neurilemoma/patología , Neoplasias del Sistema Respiratorio/clasificaciónAsunto(s)
Traumatismos de la Espalda , Traumatismos Craneocerebrales/cirugía , Congelación de Extremidades/cirugía , Cuero Cabelludo/lesiones , Dorso/cirugía , Cuidados Críticos , Humanos , Masculino , Persona de Mediana Edad , Cuidados Posoperatorios , Cuidados Preoperatorios , Cuero Cabelludo/cirugía , Trasplante de PielAsunto(s)
Abdomen Agudo/complicaciones , Complicaciones Posoperatorias/terapia , Abdomen Agudo/cirugía , Enfermedad Aguda , Humanos , Obstrucción Intestinal/etiología , Obstrucción Intestinal/prevención & control , Obstrucción Intestinal/terapia , Hepatopatías/etiología , Hepatopatías/prevención & control , Hepatopatías/terapia , Infarto del Miocardio/etiología , Infarto del Miocardio/prevención & control , Infarto del Miocardio/terapia , Pancreatitis/etiología , Pancreatitis/prevención & control , Pancreatitis/terapia , Neumonía/etiología , Neumonía/prevención & control , Neumonía/terapia , Complicaciones Posoperatorias/etiología , Complicaciones Posoperatorias/prevención & control , Dehiscencia de la Herida Operatoria/etiología , Dehiscencia de la Herida Operatoria/prevención & control , Dehiscencia de la Herida Operatoria/terapia , Tromboembolia/etiología , Tromboembolia/prevención & control , Tromboembolia/terapiaAsunto(s)
Bloqueadores Ganglionares/uso terapéutico , Cuidados Intraoperatorios , Cuidados Posoperatorios , Adulto , Anciano , Colecistectomía , Colecistitis/fisiopatología , Colecistitis/cirugía , Urgencias Médicas , Femenino , Hemodinámica/efectos de los fármacos , Humanos , Masculino , Persona de Mediana Edad , Factores de TiempoAsunto(s)
Errores Diagnósticos , Válvula Ileocecal/cirugía , Procedimientos Quirúrgicos Operativos/efectos adversos , Adolescente , Adulto , Anciano , Diagnóstico Diferencial , Femenino , Humanos , Enfermedades Intestinales/diagnóstico , Enfermedades Intestinales/cirugía , Neoplasias Intestinales/diagnóstico , Neoplasias Intestinales/cirugía , Laparoscopía , Masculino , Persona de Mediana EdadAsunto(s)
Quemaduras , Superficie Corporal , Quemaduras/diagnóstico , Quemaduras/cirugía , Humanos , MétodosRESUMEN
The clinical course and morphological changes in 33 limb joints in 20 burnt patients with pyocyanic arthritis have been studied. The latter is characterized by a grave clinical course and not infrequently complicated with sepsis which becomes especially hazardous after destruction of articular cartilages. Destructive changes in the joints would develop in different terms: following 25-45 days in large joints, in smaller one 10-15 days after the appearance of the clinical picture of this complication. Surgical therapy (arthrotomy, amputation of the extremity) associated with conservative measures (antibacterial therapy, blood transfusion, etc) should be considered to be the mostly effective treatment for destructive forms of arthritis.
Asunto(s)
Artritis Infecciosa/etiología , Quemaduras/complicaciones , Infecciones por Pseudomonas/complicaciones , Artritis Infecciosa/microbiología , Artritis Infecciosa/patología , Humanos , Pseudomonas aeruginosa/patogenicidadRESUMEN
In the paper an experience with treatment of 51 patients having burn lesions of the cranial vault bones is reported. The problems of surgical tactics in treatment of subjects with burn lesions such as terms and character of operative interventions, methods of replacement of the resulting wound defects of the cranial vault by means of various technic of skin plasty are discussed.