RESUMEN
Melanins are polyphenolic pigments of plants, animals and microbes with antioxidant and antiradiation activity. Water-soluble melanin from buckwheat is experienced as antimutagenic means (0.01-10 mg/kg per os) for cyclophosphamide (20 mg/kg i.p.) in the 3 series of experiments. The frequency of chromosome aberrations in the cells of mice bone marrow after mutagene is reduced 2-6 times once under influence of melanin.
Asunto(s)
Antimutagênicos/farmacología , Ciclofosfamida/toxicidad , Fagopyrum/química , Melaninas/farmacología , Mutágenos/toxicidad , Animales , Células de la Médula Ósea/efectos de los fármacos , Células de la Médula Ósea/ultraestructura , Aberraciones Cromosómicas/efectos de los fármacos , Masculino , Melaninas/aislamiento & purificación , Ratones , Ratones Endogámicos C57BLRESUMEN
The chromosome aberration assay in the bone marrow cells of C57BL/6 mice showed that melanin pigment (MP) in a dose range from 0.01 to 10 mg/kg does not influence the clastogenic effect of dioxidine (200 mg/kg, i.p.), while reducing the clastogenic effect of cyclophosphamide (20 mg/kg, i.p.) by a factor of 1.5-4 in various treatment regimes depending on the mutagen injection time.
Asunto(s)
Antimutagênicos/farmacología , Melaninas/farmacología , Mutágenos/toxicidad , Extractos Vegetales/farmacología , Animales , Antimutagênicos/aislamiento & purificación , Células de la Médula Ósea/efectos de los fármacos , Aberraciones Cromosómicas , Ciclofosfamida/toxicidad , Relación Dosis-Respuesta a Droga , Masculino , Melaninas/aislamiento & purificación , Ratones , Ratones Endogámicos C57BL , Extractos Vegetales/química , Quinoxalinas/toxicidadRESUMEN
Antimutagenic effects of combination of aspartame (0.4 and 4 mg/kg) and beta-carotene (0.15-15 mg/kg) were studied by estimation of chromosome aberrations in bone marrow cells of C57Bl/6 mice. Single and 5-day treatment with this combination decreased the clastogenic effects of dioxidine and cyclophosphamide and produced a more potent and universal antimutagenic effect than its constituents.
Asunto(s)
Antimutagênicos/farmacología , Aspartame/farmacología , Ciclofosfamida/toxicidad , Mutágenos/toxicidad , Quinoxalinas/toxicidad , beta Caroteno/farmacología , Animales , Antimutagênicos/administración & dosificación , Aspartame/administración & dosificación , Aberraciones Cromosómicas , Ciclofosfamida/administración & dosificación , Masculino , Ratones , Ratones Endogámicos C57BL , Mutágenos/farmacología , Quinoxalinas/administración & dosificación , beta Caroteno/administración & dosificaciónRESUMEN
The method of chromosome aberration count in the bone marrow cells of C57B1/6 mice was used to study the influence of aspartame on the cytogenetic effects of dioxydin and cyclophosphan. Aspartame (0.4-40 mg/kg) was found to possess antimutagenic properties in relation to the listed mutagens. The discovered antimutagenic activity of aspartame was manifested more when it was injected for 5 days before the administration of a mutagen, whereas in joint administration of aspartame with the mutagens, the substitute for sugar did not change the clastogenic effect of dioxydin and cyclophosphan.
Asunto(s)
Antimutagênicos/farmacología , Aspartame/farmacología , Animales , Células de la Médula Ósea/citología , Células de la Médula Ósea/efectos de los fármacos , Aberraciones Cromosómicas , Ciclofosfamida/farmacología , Relación Dosis-Respuesta a Droga , Interacciones Farmacológicas , Masculino , Metafase/efectos de los fármacos , Ratones , Ratones Endogámicos C57BL , Mutágenos/farmacología , Quinoxalinas/farmacología , Factores de TiempoRESUMEN
The influence of the food dyes E160e (beta-apo-8'-carotenal in an oil suspension) and E160a (beta-carotene in an oil suspension) on clastogenic effects of cyclophosphamide (CP) and dioxidine (DN) was investigated. Chromosome damage in the bone marrow of C57BL/6 mice was reported. The following protocols were used: (1) simultaneous single administration of the dye and the mutagen and the subsequent animal sacrifice within 24 hr; (2) a 4-day pretreatment with the dye (daily administrations) followed with simultaneous injection of the dye and the mutagen on the 5th day 24 hr before sacrifice; (3) daily co-administration of the dye and the mutagen for 5 days with sacrifice 6 hr after the last administration. CP at a dose of 30 mg/kg and DN at 300 mg/kg were injected intraperitoneally; the dyes at doses of 0.5, 5 and 50 mg/kg were given orally. Under all the protocols applied, E160e at a dose of 50 mg/kg caused a significant reduction of both DN and CP effects. At 5 mg/kg this dye reduced the effects of the mutagens only under the pretreatment regimen. Pretreatment with E160a at doses of 5 and 50 mg/kg resulted in a meaningful reduction of the DN effect. Under the combined treatment with mutagens this dye reduced both CP and DN effects.
Asunto(s)
Antimutagênicos/farmacología , Carotenoides/farmacología , Ciclofosfamida/toxicidad , Colorantes de Alimentos/farmacología , Mutágenos/farmacología , Quinoxalinas/toxicidad , beta Caroteno/farmacología , Animales , Antioxidantes/farmacología , Células de la Médula Ósea/efectos de los fármacos , Ciclofosfamida/antagonistas & inhibidores , Masculino , Ratones , Ratones Endogámicos C57BL , Pruebas de Mutagenicidad , Quinoxalinas/antagonistas & inhibidoresRESUMEN
A chromosome aberration test on bone marrow cells of C57B1/6 mice showed that beta-carotene (BC) applied by oral administration as a E160a food dye (30% oil suspension) at doses of 0.5, 5, and 50 mg/kg simultaneously with cyclophosphamide (CPA) and dioxidine (DN) injected intraperitoneally for a period of 24 h did not modify their clastogenic effects. If the animals were pretreated with perorally administrated beta-carotene dye at doses of 5 and 50 mg/kg (corresponding to 1.5 and 15 mg/kg of BC) for 5 consecutive days, a statistically significant reduction in the clastogenic effect of the DN injected for 24 h but not the CPA was observed. In another set of experiments, E160a and clastogens were administered simultaneously for 5 consecutive days, and the animals were killed 6 h after the last treatment. In this case, BC at the dose of 0.15-15 mg/kg statistically significantly reduced the clastogenicity of DN at all doses used, and of CPA at doses of 1.5 and 15 mg/kg.
Asunto(s)
Antimutagênicos/farmacología , Mutágenos/toxicidad , beta Caroteno/farmacología , Animales , Aberraciones Cromosómicas , Ciclofosfamida/toxicidad , Masculino , Ratones , Ratones Endogámicos C57BL , Mutágenos/química , Quinoxalinas/toxicidadRESUMEN
The method of chromosome aberration scoring in the bone marrow cells of C57BL/6 mice was used to study the influence of apo-carotene (AC) on the clastogenic effect of intraperitoneal injections of the following two mutagens: cyclophosphamide (CP) in a dose of 30 mg/kg and dioxidine (DN) in a dose of 300 mg/kg. AC was given per os as a food dye E160L (20% oil suspension) in doses of 0.5, 5, and 50 mg/kg (0.1, 1.0 and 10 mg/kg, respectively, on conversion to pure apo-carotene). The experiment was conducted in three variants: in simultaneous injection of the compounds for 24 h, injection of the mutagens for 24 h in 5-day treatment of the animals with AC, and in combined 5-day administration of AC and mutagens and killing of the animals 6 h after the last administration. In all variants a 10 mg/kg dose of AC reduced the clastogenic effect of CP and DN. Moreover, the clastogenic effect of CP was reduced in pretreatment of the animals with AC in a dose of 1 mg/kg, and that of DN when it was combined with AC in a dose of 0.1 mg/kg for 5 days and in pretreatment of the animals with AC in a dose of 1.0 mg/kg.
Asunto(s)
Médula Ósea/efectos de los fármacos , Carotenoides/farmacología , Aberraciones Cromosómicas/genética , Ciclofosfamida/toxicidad , Mutágenos/toxicidad , Quinoxalinas/toxicidad , Animales , Células de la Médula Ósea , Carotenoides/administración & dosificación , Ciclofosfamida/administración & dosificación , Interacciones Farmacológicas , Inyecciones Intraperitoneales , Masculino , Ratones , Ratones Endogámicos C57BL , Mutación/efectos de los fármacos , Mutación/genética , Quinoxalinas/administración & dosificaciónRESUMEN
The action of the food dyes E160e (20% beta-apo-8'-carotinale in oily suspension) and E160e (20% beta-carotene in oily suspension) on the clastogenic effects of the indirect alkylating mutagen cyclophosphamide (CP) and the prooxidative mutagen dioxidine (D) was examined by the using the chromosomal aberrations tests on the bone marrow cells from C57B1/6 mice. E160e in a dose of 50 mg/kg caused a significant decrease in the count of aberrant cells damaged by D or CP. When given in a dose 5 mg/kg, the dye reduced the effects of these mutagens only during preadministration. Moreover, E160e in a dose of 0.5 mg/kg substantially lowered the clastogenic effect of D after its 5-day use in combination with the mutagen E160a. Pretreatment of the animals with E160a in doses of 5 und 50 mg/kg caused a significant reduction in the cytogenetic effect of D. When used in combination with the mutagens, this dye in doses of 5 and 50 mg/kg reduced the clastogenic action of CP, when given in doses of 0.5, 5, and 50 mg/kg, it decreased the count of cells damaged by D. In other variants of the experiment E160a was inactive.
Asunto(s)
Carotenoides/genética , Ciclofosfamida/farmacología , Mutagénesis/efectos de los fármacos , Mutágenos/farmacología , Quinoxalinas/farmacología , Animales , Aberraciones Cromosómicas/genética , Masculino , Ratones , Ratones Endogámicos C57BL , Mutagénesis/genéticaRESUMEN
The cytogenetic activity of food dyes was examined in the experiments on male C57B1/6 mice which were orally given during 5 days the following daily doses: Tartrasine (E102), 0.5 and 5.0 mg/kg; Indigo carmine (E132), 1.4 and 14 mg/kg; Canset yellow (E110), 0.17 and 1.7 mg/kg; Cochenillerot A (E124), 0.63 and 6.3mg/kg; Azorubin (E122), 1 and 10 mg/kg and Patentblau V (E131), 0.08 and 0.8 mg/kg. Five hundred metaphase slides each were analyzed in the control and experimental test series. The findings may conclude that the dyes tested within the above dose ranges do not induce any increase in the level of cells with chromosomal damages in the inbred animals.
Asunto(s)
Aberraciones Cromosómicas , Colorantes de Alimentos/toxicidad , Animales , Masculino , Metafase , Ratones , Ratones Endogámicos C57BL , Pruebas de MutagenicidadRESUMEN
The present paper describes the possible clastogenic activity of the following synthetic sugar substitutes, such as cyclamate in daily doses of 11 and 110 mg/kg, saccharin, 5 and 50 mg/kg, acesulfam, 15 and 150 mg/kg, sucralose, 15 and 150 mg/kg, aspartame, 40 and 400 mg/kg, orally given to C57Bl/6 mice during 5 days. No clastogenic activity was found in the compounds tested.