RESUMEN

The fabrication of biosensors has different future applications mainly from the perspective of eco-friendly technologies. Label-free strategies, recyclable materials and low-temperature processing are parameters to consider for the development of a new generation of biosensor devices. In this work, Zinc oxide (ZnO) Thin-film Transistors (TFTs) using recyclable plastic substrates were used for real-time enteropathogenic Escherichia coli detection as an approach for biosensing (bio-TFTs). Fourier Transform Infrared Spectroscopy was used to verify the characteristic absorption peaks at the different steps of the bio-TFT assembly process. The bio-TFTs are ready to observe the bacterial detection by electrical characterization. Finally, detection was validated by a coupled strategy that fuses the genomic DNA extraction from bacteria attached in situ over bio-TFTs surface and, the development of the Polymerase Chain Reaction to amplify specific genes from enteropathogenic Escherichia coli.

Asunto(s)

Técnicas Biosensibles , Óxido de Zinc , ADN , Plásticos , Transistores Electrónicos

RESUMEN

The antibody immobilization compatible with low-cost materials and label-free strategies is a challenge for biosensor device fabrication. In this study, ZnO thin film deposition was carried out on corning glass substrates by ultrasonic spray pyrolysis at 200 °C. The thin films were analyzed as platforms for enteropathogenic Escherichia coli (E. coli EPEC) antibody immobilization. The modification of thin films from the functionalization and antibody immobilization steps was visualized using Fourier transform infrared spectroscopy (FTIR) spectroscopy, and surface changes were observed by atomic force microscopy. The obtained FTIR spectra after functionalization showed a contribution of the amino group (NH2) derived from silane (3-aminopropyltrimethoxysilane). The antibody immobilization showed an amide I conserved signal corresponding to the C=O stretching vibrations and the amide II signal related to the N-H scissor vibration mode. In this way, the signals observed are correlated with the presence of antibody immobilized on the film. The ZnO film morphology changes after every stage of the process and allows observing the antibody distribution on the immobilized surface. In order to validate the antibody recognition capability as well as the E. coli EPEC detection in situ, polymerase chain reaction was used.