RESUMEN
Stroke results in varying levels of motor and sensory disability that have been linked to the neurodegeneration and neuroinflammation that occur in the infarct and peri-infarct regions within the brain. Specifically, previous research has identified a key role of the corticospinal tract in motor dysfunction and motor recovery post-stroke. Of note, neuroimaging studies have utilized magnetic resonance imaging (MRI) of the brain to describe the timeline of neurodegeneration of the corticospinal tract in tandem with motor function following a stroke. However, research has suggested that alternate motor pathways may also underlie disease progression and the degree of functional recovery post-stroke. Here, we assert that expanding neuroimaging techniques beyond the brain could expand our knowledge of alternate motor pathway structure post-stroke. In the present work, we will highlight findings that suggest that alternate motor pathways contribute to post-stroke motor dysfunction and recovery, such as the reticulospinal and rubrospinal tract. Then we review imaging and electrophysiological techniques that evaluate alternate motor pathways in populations of stroke and other neurodegenerative disorders. We will then outline and describe spinal cord neuroimaging techniques being used in other neurodegenerative disorders that may provide insight into alternate motor pathways post-stroke.
RESUMEN
BACKGROUND: Alcohol abuse and alcoholism are significant global issues. Honey bees are excellent models for learning and other complex behaviors; furthermore, they share many behavioral responses to ethanol (EtOH) with humans and animal models. We develop a 2-feeder choice assay to determine whether honey bees will self-administer and preferentially consume solutions containing EtOH. METHODS: Gustatory responsiveness to EtOH is determined using the proboscis extension reflex and consumption assays. A 2-feeder choice assay is used to examine preference for the consumption of EtOH. Survival assays assess the metabolic and toxic effects of EtOH consumption. RESULTS: Honey bees find the taste of EtOH to be aversive when in water, but addition of sucrose masks the aversive taste. Even though the taste of EtOH is not appetitive, honey bees preferentially consume sucrose solutions containing 1.25 to 2.5% EtOH in a dose-dependent manner. Based on survival assays, honey bees may not be able to derive caloric value from EtOH, and EtOH concentrations of 2.5% or higher lead to significant increases in mortality. CONCLUSIONS: Honey bees will self-administer EtOH and show a preference for consuming solutions containing EtOH. Bees may not be able to efficiently utilize EtOH as an energy source, but EtOH-dependent increases in mortality complicate separating the effects of caloric value and toxicity.