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This study evaluated the photoactivation of hydrogen peroxide gels at different concentrations using blue or violet LED in terms of whitening efficacy and tooth sensitivity. Forty patients were randomly divided into 4 groups: HP6V (violet LED and 6% hydrogen peroxide), HP6B (blue LED and 6% hydrogen peroxide), HP35V (violet LED and 35% hydrogen peroxide), and HP35B (blue LED and 35% hydrogen peroxide). The L*, a* and b* values were measured before, 1 week and 3 months after treatment, and the ΔE and ΔWID values were calculated. Tooth sensitivity was measured using a visual analogue scale (VAS) before, immediately after, and 24 h after bleaching. The ΔE, ΔWID and bleaching sensitivity values were subjected to the ANOVA test and Bonferroni post-test. HP35V and HP35B showed higher whitening efficacy than HP6VL, while HP6V did not show statistical differences compared to the other groups. Regarding bleaching-related sensitivity, the HP6V and HP6B groups presented the lowest values when compared to HP35V and HP35B. HP6V showed whitening efficacy comparable to HP35V and HP35B but with reduced tooth sensitivity. TRIAL REGISTRATION NUMBER: NCT06165458; registration date: 12/09/2023.
Asunto(s)
Sensibilidad de la Dentina , Peróxido de Hidrógeno , Blanqueadores Dentales , Blanqueamiento de Dientes , Humanos , Blanqueamiento de Dientes/métodos , Blanqueamiento de Dientes/instrumentación , Femenino , Adulto , Blanqueadores Dentales/administración & dosificación , Masculino , Adulto Joven , Persona de Mediana Edad , Resultado del TratamientoRESUMEN
Objectives: This study aimed to evaluate in vitro the effects of the self-adhesive resin cements RelyX U200 (3M ESPE) and seT PP (SDI Limited) on murine macrophages and the interference of the photoactivation. Materials and Methods: Cell viability assays, cell adherence, yeast phagocytosis of Saccharomyces boulardii and production of reactive oxygen species (ROS) were performed in the presence of capillaries containing the respective self-adhesive cement when photoactivated or not. Results: After long periods of contact, both types of cements, when not photoactivated, are more cytotoxic for macrophages. The seT PP cement when only chemically activated seems to interfere more negatively in the process of phagocytosis of yeasts S. boulardii. Both types of cements interfere in the cell adhesion process, independent of photoactivation. None of the types of cements tested was able to induce the production of ROS. Conclusions: Our results highlight the great importance of the photoactivation of self-adhesive resin cements in the dental clinic, since RelyX U200, when photoactivated, presented the best results within the evaluated parameters.
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Aim: To compare the influence of prophylaxis with sodium bicarbonate and amino acid glycine powder on the bond strength of bovine teeth enamel and on the properties of two adhesive systems. Methods:Thirty-six extracted bovine incisors were randomly divided into six groups (n = 6) according to the prophylactic treatment received: no prophylactic treatment (NT), sodium bicarbonate powder (SB), and glycine powder (GL). Each group was subdivided into 2 groups based on what adhesive systems were used: conventional system (A) and universal system (B). Composite resin was applied on the buccal surface of the teeth in a block measurement 8x8x6 mm. The specimens were cut to obtain beams measuring 1.0 x 1.0 mm and were subjected to microtensile bond strength tests. Results were compared using two-way ANOVA (p ≤ 0.05). Results: The GLA group obtained the highest bond strength value for the conventional adhesive (18.97 MPa), but the GLB group obtained a lower strength value than the SBB group (GLB: 21.05 MPa and SBB: 22.29 MPa) (p < 0.05). Conclusions: Cleaning of the enamel surface increases the adhesive properties of restorative materials, and the bond strength was more effective in the group that received glycine prophylaxis and the conventional adhesive system.
Objetivo:Comparar a influência da profilaxia com bicarbonato de sódio e ClinproProphy ™ na resistência de união do esmalte de dentes bovinos e nas propriedades de dois sistemas adesivos.Métodos: Trinta e seis incisivos bovinos extraídos foram divididos aleatoriamente em 6 grupos (n = 6), cada um de acordo com o tratamento profilático recebido: nenhum tratamento profilático (NT), bicarbonato de sódio em pó (SB), glicina em pó (GL). Cada grupo foi subdividido em 2 grupos com base nos sistemas adesivos utilizados: sistema convencional (A) e sistema universal (B). A resina composta foi aplicada na superfície bucal dos dentes em um bloco de 8x8x6 mm. As amostras foram cortadas para obter blocos medindo 1,0 x 1,0 mm e submetidas a testes de resistência de união por microtração. Os resultados foram comparados usando o teste two-way ANOVA (p ≤ 0,05). Resultados: O grupo GLA obteve o maior valor de resistência de união para o adesivo convencional (18,97 MPa), mas o grupo GLB obteve um valor de resistência menor que o grupo SBB (GLB: 21,05 MPa e SBB: 22,29 MPa) (p < 0,05). Conclusão: A limpeza da superfície do esmalte aumenta as propriedades adesivas dos materiais restauradores, e a resistência adesiva foi mais eficaz no grupo que recebeu profilaxia com glicina e sistema adesivo convencional.
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Resistencia a la Tracción , Recubrimiento Dental Adhesivo , Esmalte Dental , Profilaxis Dental , GlicinaRESUMEN
A PoliÌtica Nacional de HumanizaçaÌo (PNH) foi criada em 2003 pelo MinisteÌrio da SauÌde, em meio aÌnecessidade de se ampliar o convencional modelo sanitaÌrio baseado no eixo doença-cura para um modelo que abordasse, de maneira coletiva, a famiÌlia e a comunidade. A modificaçaÌo das estrateÌgias de atuaçaÌo/atendimento e da gestãodo Sistema Único de Saúde (SUS)tornou-se, então, uma prioridade. O objetivo desse artigo foi relatar a implementaçaÌo das praÌticas de acolhimento para os usuaÌrios que buscam atendimento cliÌnico na Faculdade de Odontologia daUniversidade Federal de Minas Gerais(FAO-UFMG). A partir dacriação do projeto de extensaÌo "Acolhimento e OrientaçaÌo ao UsuaÌrioda FAO-UFMG",em agosto de 2017, dados quantitativosrelacionados à queixa principal e encaminhamento dos pacientes foram coletadosdas fichas clínicas específicas do projeto.Da análise desses dados, foi possível perceber que os usuários passaram a ser atendidos de forma mais humanizada, acolhedora, respeitosa e com maior qualidade, tendo sua trajetória terapêutica mais bem delineada. As ações implementadastambém repercutirampositivamentena logística de funcionamento do setor responsável pela recepção dos pacientes, nocuidado prestado pelos profissionais envolvidos na atenção à saúdee no aprendizadodos alunos, contribuindo para a consolidação do projeto,pautado nos pilares da extensão (AU).
The National Humanization Policy was created in 2003 by the Ministry of Health. This policy emerged due to the need to expand the health model based on the disease-healing axisclassification, to a model that collectively addressed the family and the community, respecting the context in which they live and theirlife experiences.Consequently, this led tothe change inthe action/attendance strategies and the SUS management becominga priority. The aim of this article was to report the implementation of welcoming practices for users seeking clinical care at the Schoolof Dentistry of Federal University of Minas Gerais (FAO-UFMG). Based onthe creation of the extension project "Welcomingand User Guidance ofFAO-UFMG,"in August 2017, quantitative data related to the main complaint and referral of patients were collected from the specific clinicalfiles of the project. Analysis of these datamade itpossible to note that users beganto be cared for in a morehumanized, welcoming, and respectfulmanner, with higher quality, and improved delineation of their therapeutic trajectory. The actions implemented also had a positive impact on theoperationallogistics of the sector responsible for receiving patients, on the care provided by the professionals involved in health care and on student learning, thereby contributing to the consolidation of the project, based on the pillars of extension (AU).
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Humanos , Masculino , Femenino , Sistema Único de Salud , Relaciones Comunidad-Institución , Odontología , Humanización de la Atención , Acogimiento , Atención Primaria de SaludRESUMEN
A 41-year-old male with a dental history of invasive cervical resorption (ICR) was initially treated with a surgical endodontics approach and secondly with antimicrobial photodynamic therapy (aPDT) along with endodontic retreatment. The use of aPDT was essential to promote bacterial reduction in the resorption defect. Combining these techniques allowed for clinical, radiographic, and tomographic success after five years of follow-up.
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Antiinfecciosos , Fotoquimioterapia , Adulto , Tomografía Computarizada de Haz Cónico , Estudios de Seguimiento , Humanos , Masculino , Fotoquimioterapia/métodos , Fármacos Fotosensibilizantes/uso terapéuticoRESUMEN
The aim of this study was to evaluate the gene expression of proinflammatory (RANKL, TNF-a and IFN-g) and regulatory (TGF-b and IL-10) cytokines as reaction to experimental infection by mono or bi-association of Fusobacterium nucleatum (ATCC 10953) and Enterococcus faecalis (ATCC 19433). F. nucleatum and E. faecalis, either in mono- or bi-association were inoculated into the root canal system (RCS) of Balb/c mice. Animals were sacrificed at 10 and 20 days after infection and periapical tissues surrounding the root were collected. The mRNA expression of the cytokines RANKL, TNF-a, IFN- g, TGF-b and IL-10 was assessed using real-time PCR. The Kruskal-Wallis test was used for statistical analysis. F. nucleatum mono-infection induced high expression of RANKL and TNF-a, while its modulation was due to IL-10. High expression of IFN-g at day 20 was up-regulated by E. faecalis and RANKL; TNF-a was up-regulated by an independent mechanism via IL-10 and TGF-b. Bi-association (F. nucleatum and E. faecalis) stimulated high expression of RANKL, TNF-a and IFN-g, which seemed to be modulated by TGF-b 20 days later. The gene expression of proinflammatory cytokines was more prominent in the earlier periods of the experimental periapical infection, which concomitantly decreased in the later period. This expression may be regulated by IL-10 and TGF-b in an infection-specific condition.
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Citocinas/metabolismo , Cavidad Pulpar/patología , Enterobacter/metabolismo , Fusobacterium nucleatum/metabolismo , Animales , Cavidad Pulpar/metabolismo , Ratones , Ratones Endogámicos BALB CRESUMEN
Abstract The aim of this study was to evaluate the gene expression of proinflammatory (RANKL, TNF-a and IFN-g) and regulatory (TGF-b and IL-10) cytokines as reaction to experimental infection by mono or bi-association of Fusobacterium nucleatum (ATCC 10953) and Enterococcus faecalis (ATCC 19433). F. nucleatum and E. faecalis, either in mono- or bi-association were inoculated into the root canal system (RCS) of Balb/c mice. Animals were sacrificed at 10 and 20 days after infection and periapical tissues surrounding the root were collected. The mRNA expression of the cytokines RANKL, TNF-a, IFN- g, TGF-b and IL-10 was assessed using real-time PCR. The Kruskal-Wallis test was used for statistical analysis. F. nucleatum mono-infection induced high expression of RANKL and TNF-a, while its modulation was due to IL-10. High expression of IFN-g at day 20 was up-regulated by E. faecalis and RANKL; TNF-a was up-regulated by an independent mechanism via IL-10 and TGF-b. Bi-association (F. nucleatum and E. faecalis) stimulated high expression of RANKL, TNF-a and IFN-g, which seemed to be modulated by TGF-b 20 days later. The gene expression of proinflammatory cytokines was more prominent in the earlier periods of the experimental periapical infection, which concomitantly decreased in the later period. This expression may be regulated by IL-10 and TGF-b in an infection-specific condition
Resumo O objetivo deste trabalho foi avaliar a expressão gênica de citocinas pró-inflamatórias (RANKL, TNF-a e IFN-g) e regulatórias (TGF-b e IL-10) em resposta à infecção experimental por Fusobacterium nucleatum (ATCC 10953) e Enterococcus faecalis (ATCC 19433) como mono-infecção ou em bi-associação. F. nucleatum e E. faecalis foram inoculados no sistema de canais radiculares de camundongos Balb/c, tanto isoladas como em bi-associação. Os animais foram sacrificados em 10 e 20 dias após a infecção, e os tecidos periapicais foram coletados. As expressões do mRNA das citocinas RANKL, TNF-a, IFN-g, TGF-b e IL-10 foram analisadas por meio do real-time PCR. O teste de Kruskal-Wallis foi utilizado para análise estatística. A mono-infecção com F. nucleatum induziu alta expressão de RANKL e TNF-a, enquanto sua modulação ocorreu devido à IL-10. A alta expressão de IFN-g no dia 20 foi regulada positivamente por E. faecalis e RANKL; TNF-a foi regulada positivamente por um mecanismo independente via IL-10 e TGF-b. A bi-associação (F. nucleatum e E. faecalis) estimulou uma alta expressão de RANKL, TNF-a e IFN-g, que parece ser modulada por TGF-b após 20 dias. A expressão gênica de citocinas pró-inflamatórias foi mais proeminente nos estágios iniciais da infecção periapical experimental, com concomitante redução no período tardio. Este fenômeno pode ser regulado por IL-10 e TGF-b em uma condição de infecção específica.
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Animales , Citocinas/metabolismo , Cavidad Pulpar/patología , Enterobacter/metabolismo , Fusobacterium nucleatum/metabolismo , Cavidad Pulpar/metabolismo , Ratones , Ratones Endogámicos BALB CRESUMEN
AIM: To assess the influence of co-culture with mineral trioxide aggregate (MTA) and MTA Fillapex (FLPX) on the viability, adherence, and phagocytosis activity of peritoneal macrophages from two mouse strains. METHODOLOGY: Cellular viability, adherence, and phagocytosis of Saccharomyces boulardii were assayed in the presence of capillaries containing MTA and MTA Fillapex. The data were analyzed using parametric (Student's t) and non-parametric (Mann-Whitney) tests. RESULTS: FLPX was severely cytotoxic and decreased cell viability, adherence, and phagocytic activity of both macrophage subtypes. Cells that were treated with MTA Fillapex remained viable (>80%) for only 4 h after stimulation. Macrophages from C57BL/6 mice presented higher adherence and higher phagocytic activity compared with macrophages from BALB/c mice. CONCLUSION: Comparison of MTA and FLPX effects upon macrophages indicates that FLPX may impair macrophage activity and viability, while MTA seems to increase phagocytic activity.
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Compuestos de Aluminio/toxicidad , Compuestos de Calcio/toxicidad , Macrófagos/efectos de los fármacos , Óxidos/toxicidad , Materiales de Obturación del Conducto Radicular/toxicidad , Silicatos/toxicidad , Animales , Materiales Biocompatibles/toxicidad , Supervivencia Celular/efectos de los fármacos , Técnicas de Cocultivo , Combinación de Medicamentos , Ensayo de Materiales , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Fagocitosis/efectos de los fármacos , Saccharomyces boulardiiRESUMEN
Invasive cervical resorption is an external resorption that begins below the epithelial attachment. It is caused primarily by dental trauma, orthodontic treatment, or dental bleaching. This case report involved an invasive Class III cervical resorption resulting from trauma to the superior right central incisor. Root canal treatment was followed by surgical intervention. The resorptive defect was debrided, and part of the tooth was restored with resin-modified glass ionomer cement. Postoperative follow-up revealed complete healing and healthy gingival attachment.
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Cementos de Ionómero Vítreo , Cementos de Resina , Cuello del Diente/cirugía , Resorción Dentaria/cirugía , Humanos , Cuello del Diente/patologíaRESUMEN
INTRODUCTION: Root canal treatment typically involves cleaning and shaping procedures followed by treatment with antibacterial endodontic dressing between appointments and, ultimately, 3-dimensional,hermetic filling. Chlorhexidine (CHX) is effective as an irrigation solution and is used as an endodontic dressing. The aim of this study was to examine the influence of CHX on periapical cytokine expression. METHODS: Expression levels of the cytokines interferon γ, tumor necrosis factor α, interleukin (IL)-1ß, IL-17A, IL-10, and the chemokine monocyte chemoattractant protein-1 (CCL2/MCP-1) were assayed by real-time polymerase chain reaction immediately after root canal cleaning and 15 days later. RESULTS: Messenger RNA expression of IL-1ß, interferon γ, IL-10, and CCL2/MCP-1 was increased on day 15 in teeth without endodontic dressing. No statistical change was observed in the messenger RNA expression of cytokines when comparing sampling times for teeth that received endodontic dressing. CONCLUSIONS: The results show that CHX application between appointments prevented the increase of both proinflammatory and immunoregulatory cytokines 15 days after the dental procedure.
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Clorhexidina/farmacología , Citocinas/efectos de los fármacos , Tejido Periapical/efectos de los fármacos , Irrigantes del Conducto Radicular/farmacología , Preparación del Conducto Radicular/métodos , Diente/efectos de los fármacos , Quimiocina CCL2/análisis , Citocinas/análisis , Necrosis de la Pulpa Dental/inmunología , Necrosis de la Pulpa Dental/terapia , Estudios de Seguimiento , Humanos , Mediadores de Inflamación/análisis , Interferón gamma/efectos de los fármacos , Interleucina-1/análisis , Interleucina-10/análisis , Interleucina-17/análisis , Tejido Periapical/inmunología , ARN Mensajero/efectos de los fármacos , Diente/inmunologíaRESUMEN
Embora eficientes quanto ao aspecto estético, as técnicas de clareamento endógeno ainda apresentam graus variáveis de toxidade e muitas vezes podem levar ao desenvolvimento de reabsorção cervical inflamatória. Neste estudo, avaliou-se a resposta imune aos efeitos da aplicação de agentes clareadores tradicionalmente utilizados na clínica, assim como os de agentes reparadores utilizados no tratamento destas alterações. Inoculou-se, os agentes clareadores, perborato de sódio misturado ao peróxido de hidrogênio...
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Animales , Ratones , Blanqueadores Dentales/administración & dosificación , Osteoclastos , Peróxidos/administración & dosificación , Resorción Radicular/inducido químicamente , Inmunidad Mucosa , Resorción Ósea/terapiaRESUMEN
This study assessed the influence of mineral trioxide aggregate (MTA) on adaptive immune responses. BALB/c mice were immunized with heat-killed Fusobacterium nucleatum (Fn) in MTA or other control adjuvants, and serum IgG responses to Fn were measured. Either Fn- or Peptostreptococcus anaerobius (Pa)-reactive memory T cells (Tm) were preincubated in vitro with/without MTA and restimulated with Fn or Pa. Tm proliferation and cytokine production were assessed. Compared with control groups, immunoglobulin G-antibody responses were upregulated in mice immunized with Fn in MTA in a similar manner to animals immunized with Fn in Freund's adjuvant or aluminum hydroxide adjuvant. Although MTA did not affect the upregulated expression of interleukin 10, tumor necrosis factor alpha, or RANKL by Tm, it suppressed the proliferation of Pa- or Fn-Tm and inhibited their production of Th1- or Th2-signature cytokines. MTA upregulated the adaptive humoral immune responses but had little or no effect on pro- or anti-inflammatory cytokine production by Tm.
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Compuestos de Aluminio/farmacología , Formación de Anticuerpos/efectos de los fármacos , Compuestos de Calcio/farmacología , Memoria Inmunológica/efectos de los fármacos , Óxidos/farmacología , Materiales de Obturación del Conducto Radicular/farmacología , Silicatos/farmacología , Subgrupos de Linfocitos T/efectos de los fármacos , Linfocitos T Colaboradores-Inductores/efectos de los fármacos , Animales , Anticuerpos Antibacterianos/biosíntesis , Anticuerpos Antibacterianos/sangre , Antígenos Bacterianos/inmunología , Combinación de Medicamentos , Fusobacterium nucleatum/inmunología , Inmunoglobulina G/biosíntesis , Inmunoglobulina G/sangre , Interferón gamma/biosíntesis , Interleucina-10/biosíntesis , Interleucina-4/biosíntesis , Masculino , Ratones , Ratones Endogámicos BALB C , Peptostreptococcus/inmunología , Ligando RANK/biosíntesis , Subgrupos de Linfocitos T/metabolismo , Linfocitos T Colaboradores-Inductores/metabolismo , Factor de Necrosis Tumoral alfa/biosíntesisRESUMEN
OBJECTIVE: The aim of this study was to verify the profile of Candida species responsible for colonizing and infecting the oral cavity of Brazilian patients undergoing radiotherapy (RT). STUDY DESIGN: Saliva samples were collected from 21 patients before, during, and immediately after RT. Each sample was distributed in agar Sabouraud dextrose/chlorophenicol and incubated at 37 degrees C for 18 hours. Emerging colonies were identified biochemically and through the germinative tube test. Patients were examined weekly to identify clinical candidiasis. RESULTS: Candida colonization continuously increased during RT. Infection occurred in 52% of the patients, and baseline colonization was higher in infected patients. A shift toward non-albicans species was observed in both infected and noninfected patients. CONCLUSIONS: RT leads to increased colonization and infection by Candida. The shift toward non-albicans species was unrelated to antifungal therapy. There may be epidemiological differences between infected and noninfected patients.