Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Más filtros











Base de datos
Intervalo de año de publicación
1.
Carbohydr Polym ; 156: 417-426, 2017 Jan 20.
Artículo en Inglés | MEDLINE | ID: mdl-27842841

RESUMEN

This study aimed to compare two nanofiber drug delivery systems that were prepared with an electrospun process and have the potential to serve as adjuvants for the treatment of periodontal disease. The first system was composed of polycaprolactone loaded with tetracycline (TCN) and the second was composed of polycaprolactone loaded with tetracycline/ß-cyclodextrin (TCN:BCD). An antimicrobial diffusion test was performed for each of these sets of nanofibers with the microorganisms, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis, both of which contribute to periodontal disease. In vitro release profiles were also obtained, and the nanofibers were characterized by thermal analysis, x-ray powder diffraction, infrared absorption spectroscopy, and scanning electron microscopy. Profiles of the TCN and TCN:BCD nanofibers showed that drug release occurred for up to 14days. However, the TCN:BCD nanofibers appeared to better protect and enhance the biological absorption of TCN due to the formation of a TCN:BCD inclusion complex.


Asunto(s)
Aggregatibacter/efectos de los fármacos , Nanofibras/química , Porphyromonas/efectos de los fármacos , Tetraciclina/química , Tetraciclina/farmacología , beta-Ciclodextrinas/química , beta-Ciclodextrinas/farmacología , Antiinfecciosos/química , Antiinfecciosos/farmacología , Pruebas de Sensibilidad Microbiana
2.
Colloids Surf B Biointerfaces ; 136: 248-55, 2015 Dec 01.
Artículo en Inglés | MEDLINE | ID: mdl-26402423

RESUMEN

The objective of this study was to evaluate the in vivo anti-inflammatory angiogenesis activity and in vitro cytotoxicity on normal and cancer cell models of a drug delivery system consisting of poly(lactic-co-glycolic acid) nanofibers loaded with daunorubicin (PLGA-DNR) that were fabricated using an electrospinning process. The PLGA-DNR nanofibers were also characterized by thermogravimetric analysis (TGA), differential thermal analysis (DTA) and differential scanning calorimetry (DSC), X-ray diffraction (XRD), scanning electron microscopy (SEM) and confocal fluorescence microscopy. In vitro release of DNR from the nanofibers and its corresponding mechanism were also evaluated. Sixty-five percent of the DNR was released in an initial burst over 8h, and by 1224 h, eighty-five percent of the DNR had been released. The Higuchi model yielded the best fit to the DNR release profile over the first 8h, and the corresponding data from 24 to 1224 h could be modeled using zero-order kinetics. The PLGA-DNR nanofibers exhibited a higher cytotoxicity to A431 cells than free DNR but a cytotoxicity similar to free DNR against fibroblast cells. A higher antiangiogenic effect of PLGA nanofibers was observed in the in vivo data when compared to free DNR, and no inflammatory potential was observed for the nanofibers.


Asunto(s)
Antibióticos Antineoplásicos/farmacología , Daunorrubicina/farmacología , Ácido Láctico/química , Nanofibras , Ácido Poliglicólico/química , Animales , Línea Celular , Línea Celular Tumoral , Humanos , Masculino , Ratones , Microscopía Electrónica de Rastreo , Microscopía Fluorescente , Copolímero de Ácido Poliláctico-Ácido Poliglicólico , Difracción de Rayos X
3.
Mater Sci Eng C Mater Biol Appl ; 54: 252-61, 2015 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-26046289

RESUMEN

Herein, we used an electrospinning process to develop highly efficacious and hydrophobic coaxial nanofibers based on poly-cyclodextrin (polyCD) associated with poly(methacrylic acid) (PMAA) that combines polymeric and supramolecular features for modulating the release of the hydrophilic drug, propranolol hydrochloride (PROP). For this purpose, polyCD was synthesized and characterized, and its biocompatibility was assessed using fibroblast cytotoxicity tests. Moreover, the interactions between the guest PROP molecule and both polyCD and ßCD were found to be spontaneous. Subsequently, PROP was encapsulated in uniaxial and coaxial polyCD/PMAA nanofibers. A lower PROP burst effect (reduction of approximately 50%) and higher modulation were observed from the coaxial than from the uniaxial fibers. Thus, the coaxial nanofibers could potentially be a useful strategy for developing a controlled release system for hydrophilic molecules.


Asunto(s)
Celulosa/química , Ciclodextrinas/química , Sistemas de Liberación de Medicamentos/métodos , Nanofibras/química , Ácidos Polimetacrílicos/química , Materiales Biocompatibles/química , Células Cultivadas , Células Inmovilizadas , Fibroblastos/citología , Fibroblastos/efectos de los fármacos , Encía/citología , Encía/efectos de los fármacos , Humanos , Interacciones Hidrofóbicas e Hidrofílicas , Espectroscopía de Resonancia Magnética , Microscopía Electrónica de Transmisión
5.
Int J Pharm ; 336(1): 75-81, 2007 May 04.
Artículo en Inglés | MEDLINE | ID: mdl-17174495

RESUMEN

The aim of this study was to evaluate the biocompatibility of composites of poly-lactic acid polymer (PLA) and copolymer of lactic and glycolic acid (PLGA), dispersed in a bioceramic matrix, Osteosynt (BC), to which tetracycline (TC) was added. The in vitro test used direct contact test (ASTM F-813) and elution test (USP-XXIII, ISO 10993-5), and in vivo evaluation was performed after subcutaneous implantation in outbread Swiss mice. The 0.01% (w/w) TC addition did not affect composite cytotoxicity in vitro. The macroscopic and histologic evaluation in vivo after 1, 7, 13, 21, 28 and 56 days showed an initial intense infiltrate of inflammatory cells for most of the groups. The tissue showed normal pattern after 21 days for all the groups. TC addition exhibited significantly larger reduction of inflammation signs (Mann-Whitney test, p<0.05) in the critical period of the resolution of the inflammatory process. Angiogenesis, cellular adsorption and fibrous deposit were observed on SEM evaluation. In conclusion, TC addition optimized composites polymer/bioceramic biocompatibility, contributing to anti-inflammatory response during the early phases of the wound healing process.


Asunto(s)
Sustitutos de Huesos/química , Cerámica/química , Polímeros/química , Tetraciclina/química , Animales , Sustitutos de Huesos/metabolismo , Sustitutos de Huesos/farmacología , Fosfatos de Calcio/química , Adhesión Celular/efectos de los fármacos , Línea Celular , Supervivencia Celular/efectos de los fármacos , Cerámica/farmacología , Durapatita/química , Fibroblastos/citología , Fibroblastos/efectos de los fármacos , Implantes Experimentales , Inflamación/patología , Inflamación/prevención & control , Ácido Láctico/química , Ácido Láctico/farmacología , Masculino , Ensayo de Materiales , Ratones , Microscopía Electrónica de Rastreo , Poliésteres , Ácido Poliglicólico/química , Ácido Poliglicólico/farmacología , Copolímero de Ácido Poliláctico-Ácido Poliglicólico , Polímeros/farmacología , Porosidad , Tejido Subcutáneo/efectos de los fármacos , Tejido Subcutáneo/patología , Propiedades de Superficie , Tetraciclina/farmacología
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA