RESUMEN

The use of residues from coffee production to obtain biochar is a sustainable approach, which aims to minimize the environmental impact of these materials. In this study, the effect of adding coffee straw biochar on the physiological quality of lettuce and sorghum seeds was investigated. Thus, the objective of this work was to study the effect of adding different concentrations of coffee biochar in the substrate composition on the physiological quality of lettuce (Lactuca sativa) and sorghum (Sorghum bicolor) seeds. The experimental design used was completely randomized, with five concentrations of biochar (0; 7.5; 15; 30 and 60%), conducted with four replications of 25 seeds. The use of biochar in the concentrations studied does not provide an increase in the average germination percentage and vigor of lettuce and sorghum seeds. The increase in the concentration of biochar caused less seed vigor, suggesting a toxic effect. For seed germination, there was no significant difference between lettuce and sorghum species, regardless of treatment. For the germination speed index, sorghum seeds have higher means, except for the treatment with the addition of 15% coffee straw biochar. Lettuce seeds have higher shoot length averages, except for treatment with 100% commercial substrate. The sorghum seeds have higher mean root length and dry mass than lettuce, regardless of the treatment.

Asunto(s)

Carbón Orgánico , Germinación , Sorghum , Café , Grano Comestible , Lactuca , Semillas/fisiología

RESUMEN

Lung cancer patients with COVID-19 present an increased risk of developing severe disease and, consequently, have poor outcomes. Determining SARS-CoV-2-host interactome in lung cancer cells and tissues, infected or uninfected with SARS-CoV-2, may reveal molecular mechanisms associated with COVID-19 development and severity in lung cancer patients. Here, we integrated transcriptome data of lung tumors from patients with small- or non-small cell lung cancer (SCLC and NSCLC) and normal lung and lung cancer cells infected with SARS-CoV-2. We aimed to characterize molecular mechanisms potentially associated with COVID-19 development and severity in lung cancer patients and to predict the SARS-CoV-2-host cell interactome. We found that the gene expression profiles of lung cell lines infected with SARS-CoV-2 resemble more primary lung tumors than non-malignant lung tissues. In addition, the transcriptomic-based interactome analysis of SCLC and NSCLC revealed increased expression of cancer genes BRCA1 and CENPF, whose proteins are known or predicted to interact with the SARS-CoV-2 spike glycoprotein and helicase, respectively. We also found that TRIB3, a gene coding a putative host-SARS-CoV-2 interacting protein associated with COVID-19 infection, is co-expressed with the up-regulated genes MTHFD2, ADM2, and GPT2 in all tested conditions. Our analysis identified biological processes such as amino acid metabolism and angiogenesis and 22 host mediators of SARS-CoV-2 infection and replication that may contribute to the development and severity of COVID-19 in lung cancers.

Asunto(s)

COVID-19 , Carcinoma de Pulmón de Células no Pequeñas , Neoplasias Pulmonares , Transcriptoma , Humanos , Carcinoma de Pulmón de Células no Pequeñas/genética , Carcinoma de Pulmón de Células no Pequeñas/patología , COVID-19/genética , COVID-19/patología , Pulmón/metabolismo , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/patología , SARS-CoV-2

RESUMEN

We have synthesized anthracene and metal@anthracene core-shell nanoparticles to combine radiation (RT) and photodynamic (PDT) therapies. Synthesis of anthracene nanoparticles in the presence of colloidal silver or gold reduced the nanoparticles hydrodynamic radius, caused core-shell nanostructures to grow, and led to plasmon-enhanced fluorescence. Singlet oxygen (1O2) generation was investigated by electron spin resonance (ESR) and fluorescence spectroscopies. In the presence of a porphyrin, anthracene nanoparticles and the core-shell nanoparticles acted as energy mediators and increased 1O2 generation under exposure to light, as evidenced by the ESR results. Fluorescence suppression experiments showed that the core-shell nanoparticles captured 1O2 at rates higher than anthracene nanoparticles, suggesting that overall production of 1O2 (1O2 captured by spin-trap + 1O2 captured by surface anthracene molecules) was higher for the core-shell nanoparticles. Moreover, the Ag@anthracene nanoparticles stood out as a new and more sensitive fluorescent probe for 1O2. During irradiation with X-rays, both anthracene and Ag@anthracene nanoparticles trapped 1O2; subsequently, they afforded sustained release of the trapped 1O2 for up 12 days after irradiation. This could be an interesting strategy to extend the radiation therapy treatment after the irradiation sessions. Furthermore, the presence of the metallic nanoparticle in the core of the core-shell nanostructure increased interaction with X-rays, raising the radiation dose around the nanoparticle. Therefore, metal@anthracene nanostructures may allow combination of cancer treatments by different approaches depending on the adopted nanoparticle configuration.

Asunto(s)

Nanopartículas del Metal , Nanopartículas , Neoplasias , Fotoquimioterapia , Antracenos , Oro , Neoplasias/tratamiento farmacológico , Plata

RESUMEN

The genus Streptococcus comprises important pathogens, many of them are part of the human or animal microbiota. Advances in molecular genetics, taxonomic approaches and phylogenomic studies have led to the establishment of at least 100 species that have a severe impact on human health and are responsible for substantial economic losses to agriculture. The infectivity of the pathogens is linked to cell-surface components and/or secreted virulence factors. Bacteria have evolved sophisticated and multifaceted adaptation strategies to the host environment, including biofilm formation, survival within professional phagocytes, escape the host immune response, amongst others. This review focuses on virulence mechanism and zoonotic potential of Streptococcus species from pyogenic (S. agalactiae, S. pyogenes) and mitis groups (S. pneumoniae).

Asunto(s)

Infecciones Estreptocócicas , Streptococcus , Animales , Humanos , Filogenia , Streptococcus/genética , Virulencia , Factores de Virulencia/genética

RESUMEN

The present study aimed to morphometrically evaluate the ruminal mucosa and ruminal fluid characteristics of Santa Inês sheep fed different levels of spineless cactus as a replacement for Tifton grass hay. A total of 32 intact male Santa Inês sheep, approximately 180 days old and with a mean weight of 32.0±1.6kg, were studied in a completely randomized design with four treatments and eight replicates. The morphometric measurements evaluated in the ruminal epithelium (papilla height and surface area) were significantly affected (P< 0.05) by supplementation of the diet with spineless cactus, and these effects showed increasing linear trends. Evaluations of the ruminal fluid also showed a significant increasing linear effect (P< 0.05) for ammonia and a quadratic effect for microbial protein. The inclusion of spineless cactus in the sheep diet affects the ruminal epithelium morphology, including the papilla height and surface area, and favors the absorption process in the rumen. The diet containing higher levels of spineless cactus led to maximum microbial protein production. These results characterize spineless cactus as a feasible alternative for feeding sheep during periods of drought, when the typical food source is scarce.(AU)

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O presente estudo teve como objetivo avaliar morfometricamente a mucosa ruminal e as características do líquido ruminal de ovinos Santa Inês alimentados com diferentes níveis de palma forrageira como substituta do feno de capim-Tifton. Um total de 32 ovinos, machos inteiros, da raça Santa Inês, com aproximadamente 180 dias de idade e peso médio de 32,0±1,6kg, foi estudado em delineamento inteiramente ao acaso, com quatro tratamentos e oito repetições. As medidas morfométricas avaliadas no epitélio ruminal (altura e área superficial da papila) foram significativamente afetadas (P<0,05) pela suplementação da dieta com palma forrageira, e esses efeitos mostraram tendências lineares crescentes. As avaliações do fluido ruminal também mostraram um efeito linear crescente significativo (P<0,05) para a amônia e um efeito quadrático para a proteína microbiana. A inclusão de palma forrageira na dieta de ovinos afeta a morfologia do epitélio ruminal, englobando a altura e a área da papila, e favorece o processo de absorção no rúmen. A dieta contendo níveis mais altos de palma forrageira levou à produção máxima de proteína microbiana. Esses resultados caracterizam a palma forrageira como uma alternativa viável para a alimentação de ovelhas durante períodos de seca, quando a fonte típica de alimento é escassa.(AU)

Asunto(s)

Animales , Masculino , Rumen/anatomía & histología , Ovinos/anatomía & histología , Opuntia/química , Membrana Mucosa/anatomía & histología , Ácidos Grasos Volátiles

RESUMEN

O objetivo deste estudo foi avaliar o perfil de ácidos graxos (AG) do leite de vacas alimentadas com dietas à base de silagem de milho contendo 0% e 8,9% de glicerina bruta (GB) na matéria seca (MS). Foram utilizadas 18 vacas Holandês x Gir com 48±18 dias em lactação, produzindo 19,8±4,9kg/dia de leite. Utilizou-se delineamento de blocos ao acaso, com nove vacas/tratamento e duas medidas repetidas no tempo, com os resultados analisados por modelos mistos. A inclusão de GB na dieta não alterou a ingestão de MS, mas reduziu os consumos dos AG oleico, linoleico e α-linolênico. Os teores dos AG mirístico, palmítico, rumênico, vacênico, esteárico, oleico, linoleico, α-linolênico e CLA trans-10 cis-12 na gordura do leite foram semelhantes entre dietas. A inclusão de GB reduziu os teores dos AG elaídico e C18:1 trans-10 e aumentou os teores dos AG de cadeia ímpar linear e do ácido láurico. Não houve efeito da inclusão da GB sobre os índices de aterogenicidade e trombogenicidade da gordura do leite. Concluiu-se que a inclusão de glicerina bruta em dieta à base de silagem de milho não afetou a qualidade nutricional da gordura do leite de vacas Holandês x Gir.(AU)

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The objective of this study was to evaluate the milk fatty acid (FA) composition of cows fed corn silage-based diets containing 0% and 8.9% of crude glycerin (GB) on a dry matter (DM) basis. Eighteen Holstein x Gyr cows with 48±18 days in milk and producing 19.8±4.9kg milk/day were used in the study. The experimental design was a randomized block with nine cows per treatment and two repeated measures. Results were analyzed using mixed models. The milk fat contents of myristic, palmitic, rumenic, vaccenic, estearic, oleic, linoleic, α-linolenic, and trans-10 cis-12 CLA were similar between diets. Dietary inclusion of GB decreased elaidic and trans-10 C18:1, and increased lauric acid and odd linear-chain FA contents in milk fat. Both atherogenicity and trombogenicity indices were unaffected by GB inclusion. It was concluded that GB inclusion in corn silage-based diets had no effect on the nutritional quality of milk fat from Holstein x Gyr dairy cows.(AU)

Asunto(s)

Animales , Femenino , Bovinos , Ensilaje , Leche/química , Ácidos Grasos/análisis , Glicerol/administración & dosificación , Biocombustibles , Alimentación Animal

RESUMEN

O objetivo deste estudo foi avaliar o perfil de ácidos graxos (AG) do leite de vacas alimentadas com dietas à base de silagem de milho contendo 0% e 8,9% de glicerina bruta (GB) na matéria seca (MS). Foram utilizadas 18 vacas Holandês x Gir com 48±18 dias em lactação, produzindo 19,8±4,9kg/dia de leite. Utilizou-se delineamento de blocos ao acaso, com nove vacas/tratamento e duas medidas repetidas no tempo, com os resultados analisados por modelos mistos. A inclusão de GB na dieta não alterou a ingestão de MS, mas reduziu os consumos dos AG oleico, linoleico e α-linolênico. Os teores dos AG mirístico, palmítico, rumênico, vacênico, esteárico, oleico, linoleico, α-linolênico e CLA trans-10 cis-12 na gordura do leite foram semelhantes entre dietas. A inclusão de GB reduziu os teores dos AG elaídico e C18:1 trans-10 e aumentou os teores dos AG de cadeia ímpar linear e do ácido láurico. Não houve efeito da inclusão da GB sobre os índices de aterogenicidade e trombogenicidade da gordura do leite. Concluiu-se que a inclusão de glicerina bruta em dieta à base de silagem de milho não afetou a qualidade nutricional da gordura do leite de vacas Holandês x Gir.(AU)

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The objective of this study was to evaluate the milk fatty acid (FA) composition of cows fed corn silage-based diets containing 0% and 8.9% of crude glycerin (GB) on a dry matter (DM) basis. Eighteen Holstein x Gyr cows with 48±18 days in milk and producing 19.8±4.9kg milk/day were used in the study. The experimental design was a randomized block with nine cows per treatment and two repeated measures. Results were analyzed using mixed models. The milk fat contents of myristic, palmitic, rumenic, vaccenic, estearic, oleic, linoleic, α-linolenic, and trans-10 cis-12 CLA were similar between diets. Dietary inclusion of GB decreased elaidic and trans-10 C18:1, and increased lauric acid and odd linear-chain FA contents in milk fat. Both atherogenicity and trombogenicity indices were unaffected by GB inclusion. It was concluded that GB inclusion in corn silage-based diets had no effect on the nutritional quality of milk fat from Holstein x Gyr dairy cows.(AU)

Asunto(s)

Animales , Femenino , Bovinos , Ensilaje , Leche/química , Ácidos Grasos/análisis , Glicerol/administración & dosificación , Biocombustibles , Alimentación Animal

RESUMEN

At the end of 2016, Brazil experienced an unprecedented yellow fever (YF) outbreak. Clinical, molecular and ecological aspects of human and non-human primate (NHP) samples collected at the beginning of the outbreak are described in this study. Spatial distribution analyses demonstrated a strong overlap between human and NHP cases. Through molecular analyses, we showed that the outbreak had a sylvatic origin, caused by the South American genotype 1 YFV, which has already been shown to circulate in Brazil. As expected, the clusters of cases were identified in regions with a low vaccination coverage. Our findings highlight the importance of the synchronization of animal surveillance and health services to identify emerging YF cases, thereby promoting a better response to the vulnerable population.

Asunto(s)

Fiebre Amarilla/epidemiología , Virus de la Fiebre Amarilla/genética , Virus de la Fiebre Amarilla/aislamiento & purificación , Aedes/virología , Animales , Brasil/epidemiología , Enfermedades Transmisibles Emergentes , Brotes de Enfermedades , Humanos , Primates/microbiología

RESUMEN

This study aimed to evaluate the amount of fiber in the diet that is able to promote better use of nutrients in the diet of dairy goats when forage palm is the main source of roughage. Fifteen Alpine dairy goats were distributed in a 5 × 5 triple Latin square containing five treatments and five periods, and each treatment contained. We tested five diets composed of different contents of neutral detergent fiber from Tifton hay (NDFf) in the dry matter of rations containing forage palm: D1: 11.98%; D2: 18.31%; D3: 23.68%; D4: 28.76% and D5: 32.12% NDFf. The intake of dry matter, crude protein, neutral detergent fiber, non-fiber carbohydrates and ether extract decreased linearly with the increase in NDFf content. There was a linear increase of milk production and milk protein with increasing dietary NDFf. The fat, dry extract, protein, pH and acidity of the evaluated milk were not affected by increasing the levels of NDFf in the diet. In situations where dairy goats are fed forage palm and other types of forage are limited, 11.98% NDF from forage other than palm can be used as an effective source of NDF.(AU)

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Objetivou-se, com este trabalho, avaliar a quantidade de fibra na dieta que é capaz de promover melhor uso de nutrientes na dieta de cabras leiteiras quando a palma forrageira é a principal fonte de forragem. Quinze cabras leiteiras alpinas foram distribuídas em um quadrado latino triplo 5 × 5 contendo cinco tratamentos e cinco períodos. Testaram-se cinco dietas compostas por diferentes teores de fibra em detergente neutro de feno de tifton (FDNf) na matéria seca de rações contendo palma forrageira: D1: 11,98%; D2: 18,31%; D3: 23,68%; D4: 28,76% e D5: 32,12% de FDNf. A ingestão de matéria seca, proteína bruta, fibra em detergente neutro, carboidratos não fibrosos e extrato etéreo diminuiu linearmente com o aumento do conteúdo de FDNf. Houve um aumento linear da produção de leite e da proteína do leite com o aumento da FDNf dietética. A gordura, a lactose, o extrato seco, o pH e a acidez do leite não foram afetados pelo aumento dos níveis de FDNf na dieta. Em situações em que as cabras leiteiras são alimentadas com palma forrageira e outros tipos de forragem são limitados, 11,98% de FDN de forragem diferente da palma podem ser usados como fonte efetiva de fibra.(AU)

Asunto(s)

Animales , Femenino , Alimentación Animal/análisis , Cactaceae , Cabras/metabolismo

RESUMEN

This study aimed to evaluate the amount of fiber in the diet that is able to promote better use of nutrients in the diet of dairy goats when forage palm is the main source of roughage. Fifteen Alpine dairy goats were distributed in a 5 × 5 triple Latin square containing five treatments and five periods, and each treatment contained. We tested five diets composed of different contents of neutral detergent fiber from Tifton hay (NDFf) in the dry matter of rations containing forage palm: D1: 11.98%; D2: 18.31%; D3: 23.68%; D4: 28.76% and D5: 32.12% NDFf. The intake of dry matter, crude protein, neutral detergent fiber, non-fiber carbohydrates and ether extract decreased linearly with the increase in NDFf content. There was a linear increase of milk production and milk protein with increasing dietary NDFf. The fat, dry extract, protein, pH and acidity of the evaluated milk were not affected by increasing the levels of NDFf in the diet. In situations where dairy goats are fed forage palm and other types of forage are limited, 11.98% NDF from forage other than palm can be used as an effective source of NDF.(AU)

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Objetivou-se, com este trabalho, avaliar a quantidade de fibra na dieta que é capaz de promover melhor uso de nutrientes na dieta de cabras leiteiras quando a palma forrageira é a principal fonte de forragem. Quinze cabras leiteiras alpinas foram distribuídas em um quadrado latino triplo 5 × 5 contendo cinco tratamentos e cinco períodos. Testaram-se cinco dietas compostas por diferentes teores de fibra em detergente neutro de feno de tifton (FDNf) na matéria seca de rações contendo palma forrageira: D1: 11,98%; D2: 18,31%; D3: 23,68%; D4: 28,76% e D5: 32,12% de FDNf. A ingestão de matéria seca, proteína bruta, fibra em detergente neutro, carboidratos não fibrosos e extrato etéreo diminuiu linearmente com o aumento do conteúdo de FDNf. Houve um aumento linear da produção de leite e da proteína do leite com o aumento da FDNf dietética. A gordura, a lactose, o extrato seco, o pH e a acidez do leite não foram afetados pelo aumento dos níveis de FDNf na dieta. Em situações em que as cabras leiteiras são alimentadas com palma forrageira e outros tipos de forragem são limitados, 11,98% de FDN de forragem diferente da palma podem ser usados como fonte efetiva de fibra.(AU)

Asunto(s)

Animales , Femenino , Alimentación Animal/análisis , Cactaceae , Cabras/metabolismo

RESUMEN

Objetivou-se avaliar a influência de dietas contendo silagem de diferentes cultivares de sorgo sobre as características físicas e químicas na carne de ovinos sem padrão racial definido, com idade entre cinco e sete meses e peso vivo médio de 17,7±3,7kg, alimentados com dietas contendo silagem de diferentes cultivares de sorgo. O experimento foi conduzido na Estação Experimental Pendência, pertencente à Empresa Estadual de Pesquisa Agropecuária - Emepa, tendo um período experimental de 42 dias. Utilizou-se um delineamento inteiramente ao acaso, com cinco tratamentos (silagem de sorgo BRS 610, BRS 655, BRS 800, BRS 810 e Ponta Negra) e sete repetições. Ao atingirem o peso de abate de 26,24kg, os animais foram encaminhados ao abate. Procedeu-se à análise do pH, da cor, da perda de peso por cocção, da força de cisalhamento, bem como à determinação de umidade, proteínas, cinzas e lipídeos totais no músculo Longissimus dorsi. As dietas não influenciaram os parâmetros de pH (5,64), cor (L*23,13, a* 18,00, b*15,78), perdas de peso por cocção (34,43%), umidade (72,53%), proteína (24,08%), cinzas (1,09%) e lipídeos (2,99%). A força de cisalhamento diferiu entre as dietas, com a silagem do cultivar BRS 655 propiciando uma carne mais macia (2,10kgf/cm2). As silagens de sorgo utilizadas na confecção das dietas de cordeiros em confinamento não interferem nas características de qualidade da carne de ovinos sem padrão racial definido. Dietas contendo silagem de sorgo granífero BRS 655 proporcionaram uma carne mais macia.(AU)

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This study aimed to evaluate the influence of diets containing silage of different sorghum cultivars on the physical and chemical characteristics in the meat of lambs. Thirty five animals of undefined breed with average age between 5 and 7 months and average body weight of 17. 7±3.7kg were used. The lambs were randomly assigned to five treatments (sorghum silage BRS 610, BRS 655, BRS 800, BRS 810 e Ponta Negra) with seven replicates. When they reach 26.24kg of weight, the animals were sent to be slaughtered. Proceeded to the analysis of pH, color, cooking losses, shear force, determination of humidity, protein, ash and total amount of lipids in the Longissimus dorsi. The diets did not influence the parameters of pH (5.64), color (L*23.13, a* 18.00, b*15.78), cooking losses (34.43%), humidity (72.53%), protein (24.08%), ash (1.09%) or lipids (2.99%). The shear force differed among the diets, with the silage of cultivar BRS 655 providing a tender meat (2.10kgf/cm2). The sorghum silages used in in the diets did not interfere in the meat quality characteristics of feedlot lambs. Diets containing sorghum grain silage BRS 655 provided a tender meat.(AU)

Asunto(s)

Animales , Carne Roja/análisis , Ovinos , Ensilaje , Sorghum , Alimentación Animal/análisis , Fenómenos Químicos

RESUMEN

Objetivou-se avaliar a influência de dietas contendo silagem de diferentes cultivares de sorgo sobre as características físicas e químicas na carne de ovinos sem padrão racial definido, com idade entre cinco e sete meses e peso vivo médio de 17,7±3,7kg, alimentados com dietas contendo silagem de diferentes cultivares de sorgo. O experimento foi conduzido na Estação Experimental Pendência, pertencente à Empresa Estadual de Pesquisa Agropecuária - Emepa, tendo um período experimental de 42 dias. Utilizou-se um delineamento inteiramente ao acaso, com cinco tratamentos (silagem de sorgo BRS 610, BRS 655, BRS 800, BRS 810 e Ponta Negra) e sete repetições. Ao atingirem o peso de abate de 26,24kg, os animais foram encaminhados ao abate. Procedeu-se à análise do pH, da cor, da perda de peso por cocção, da força de cisalhamento, bem como à determinação de umidade, proteínas, cinzas e lipídeos totais no músculo Longissimus dorsi. As dietas não influenciaram os parâmetros de pH (5,64), cor (L*23,13, a* 18,00, b*15,78), perdas de peso por cocção (34,43%), umidade (72,53%), proteína (24,08%), cinzas (1,09%) e lipídeos (2,99%). A força de cisalhamento diferiu entre as dietas, com a silagem do cultivar BRS 655 propiciando uma carne mais macia (2,10kgf/cm2). As silagens de sorgo utilizadas na confecção das dietas de cordeiros em confinamento não interferem nas características de qualidade da carne de ovinos sem padrão racial definido. Dietas contendo silagem de sorgo granífero BRS 655 proporcionaram uma carne mais macia.(AU)

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This study aimed to evaluate the influence of diets containing silage of different sorghum cultivars on the physical and chemical characteristics in the meat of lambs. Thirty five animals of undefined breed with average age between 5 and 7 months and average body weight of 17. 7±3.7kg were used. The lambs were randomly assigned to five treatments (sorghum silage BRS 610, BRS 655, BRS 800, BRS 810 e Ponta Negra) with seven replicates. When they reach 26.24kg of weight, the animals were sent to be slaughtered. Proceeded to the analysis of pH, color, cooking losses, shear force, determination of humidity, protein, ash and total amount of lipids in the Longissimus dorsi. The diets did not influence the parameters of pH (5.64), color (L*23.13, a* 18.00, b*15.78), cooking losses (34.43%), humidity (72.53%), protein (24.08%), ash (1.09%) or lipids (2.99%). The shear force differed among the diets, with the silage of cultivar BRS 655 providing a tender meat (2.10kgf/cm2). The sorghum silages used in in the diets did not interfere in the meat quality characteristics of feedlot lambs. Diets containing sorghum grain silage BRS 655 provided a tender meat.(AU)

Asunto(s)

Animales , Carne Roja/análisis , Ovinos , Ensilaje/estadística & datos numéricos , Sorghum , Alimentación Animal/análisis , Fenómenos Químicos

RESUMEN

Sickle cell disease shows several clinical manifestations in distinct levels of severity. This heterogeneity is due to the haplotype variability associated with the HbS gene, levels of fetal hemoglobin and environmental conditions, which modify the disease expression. Science community believes that the presence of a polymorphism in the CCR5 gene, which is related to chronic inflammatory state, could confer a higher survival rate and a lower number of inflammatory events to these patients since the deletion in CCR5Δ32 would knock out the CCR5 gene. Therefore, this study aimed to identify the haplotypes in ßS and ßC genes, as well as to investigate the presence of the CCR5Δ32 deletion in patients with sickle cell disease. For this purpose, DNA was isolated with the QIAamp DNA Investigator Kit, and PCR was the method chosen to detect the mutant allele CCR5Δ32. The haplotypes in ßS and ßC genes were detected by RFLP with the restriction enzymes XmnI, HindIII, HincII, and HinfI analyzing six polymorphic sites on the ß cluster, succeeded by electrophoresis. The atypical haplotype was the most common (54.3%), followed by Benin (28.6%), Bantu (11.5%), Senegal (2.8%), and Cameroon (2.8%). No patients presented CCR5Δ32 deletion. The increase in the frequency of atypical haplotypes suggests that these patients passed by variation in the genetic pattern from ancestral haplotypes throughout the years.

Asunto(s)

Anemia de Células Falciformes/genética , Haplotipos , Polimorfismo Genético , Receptores CCR5/genética , Adolescente , Adulto , Anemia de Células Falciformes/etnología , Niño , Preescolar , Femenino , Eliminación de Gen , Hemoglobinas/genética , Humanos , Masculino , Persona de Mediana Edad

RESUMEN

BACKGROUND AND OBJECTIVE: Periodontitis may promote harmful systemic effects such as changes in hepatic tissues. The purpose of this study was to investigate whether the steatosis and oxidative stress caused by experimental periodontitis are reversible in the liver. MATERIAL AND METHODS: Twenty-four rats were divided into three groups: control, periodontitis and P20-20 (20 days with experimental periodontitis and 20 days without experimental periodontitis, to verify the reversibility of hepatic injuries). The following parameters were assessed: gingival bleeding index, probing pocket depth, myeloperoxidase activity, alveolar bone loss for periodontal tissues; liver weights, histopathological scores for steatosis, inflammation and necrosis in liver; glutathione, malondialdehyde, total cholesterol and triglyceride concentrations in hepatic tissues; and blood levels of aspartate aminotransferase, alanine aminotransferase, albumin, gamma-glutaryl transferase, total cholesterol and random glucose. RESULTS: Gingival bleeding index, probing pocket depth, myeloperoxidase and alveolar bone loss parameters demonstrated the development of periodontitis. There was a significant reduction in the steatosis score of animals from the P20-20 group when compared with the periodontitis group. P20-20 group presented significantly higher glutathione (11 times) and lower malondialdehyde (nearly 23%), total cholesterol (both in blood and hepatic tissue) and triglyceride concentrations compared with the periodontitis group. For levels of aspartate aminotransferase, alanine aminotransferase, albumin, gamma-glutaryl transferase and random glucose, a significant difference between the groups was not observed. CONCLUSION: Our results demonstrate that the microvesicular steatosis caused by periodontitis in rats is reversible after removal of the ligature, which is associated with the increase in oxidative stress and lipid peroxidation in the liver.

Asunto(s)

Hígado Graso/etiología , Hígado Graso/terapia , Ligadura/métodos , Estrés Oxidativo , Periodontitis/complicaciones , Alanina Transaminasa/sangre , Pérdida de Hueso Alveolar/clasificación , Pérdida de Hueso Alveolar/patología , Animales , Aspartato Aminotransferasas/sangre , Glucemia , Colesterol/análisis , Colesterol/sangre , Modelos Animales de Enfermedad , Hígado Graso/patología , Femenino , Encía/patología , Glutatión/análisis , Inflamación , Peroxidación de Lípido , Hígado/lesiones , Hígado/patología , Malondialdehído/análisis , Necrosis/patología , Índice Periodontal , Bolsa Periodontal/patología , Periodontitis/patología , Peroxidasa/metabolismo , Ratas , Ratas Wistar , Albúmina Sérica , Factores de Tiempo , Transaminasas/sangre , Triglicéridos/análisis , gamma-Glutamiltransferasa/sangre

RESUMEN

Abstract Oxidative stress plays a main role in the development of diabetes complications. The impairment of gonadal antioxidant potential and endocrine disturbance in diabetic males causes testicular damage and failure in sperm production. Plants have been widely used to control diabetes due their hypoglycemic and antioxidant potential, contributing towards the recovery of testicular function. Current study comprises a review of the literature on the main medicinal plants used in the recovery of testicular oxidative damage in animals with experimental diabetes. Eighteen plant species in the nineteen studies selected from the search strategy were evaluated. Plant extracts were evaluated according to their effects on blood glucose and insulin levels, antioxidant enzymes and oxidant levels, lipid peroxidation, total protein, testosterone levels, gonadosomatic index, diameter of seminiferous tubules, seminiferous epithelium height and integrity, number of germ cells at stage VII and apoptosis in the seminiferous epithelium, sperm production, motility, viability and morphology. After the analysis of the studies, it was observed that plant species, used alone or in combination, may control testicular oxidative damage triggered by diabetes. The antioxidant potential varies among species, with some plants proving to have a better performance in the recovery of reproduction parameters than others.

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Resumo O estresse oxidativo desempenha um dos principais papéis no desenvolvimento das complicações do diabetes. Em machos diabéticos, as alterações ao potencial antioxidante gonadal e os distúrbios endócrinos resultam em danos testiculares e falhas na produção de espermatozoides. Plantas têm sido amplamente utilizadas como um método de controle do diabetes devido aos seus potenciais hipoglicemiante e antioxidante, contribuindo para a recuperação da função testicular. Assim, o objetivo deste estudo é fazer uma revisão da literatura sobre as principais plantas medicinais utilizadas na recuperação de danos oxidativos testiculares em animais induzidos ao diabetes experimental. Nos dezenove estudos selecionados a partir da estratégia de busca, dezoito espécies de plantas foram utilizadas. Os extratos das espécies vegetais foram avaliados de acordo com os seus efeitos nas concentrações de glicose e insulina, níveis das enzimas antioxidantes e dos oxidantes, peroxidação lipídica, quantidade de proteínas totais, níveis de testosterona, índice gonadossomático, diâmetro dos túbulos seminíferos, altura e integridade do epitélio seminífero, número de células germinativas no estágio VII, número de células em apoptose no epitélio seminífero, produção, motilidade, viabilidade e morfologia espermática. Depois das análises aos estudos, foi observado que as espécies vegetais, sozinhas ou em combinação, podem ajudar a controlar os danos oxidativos testiculares desencadeados pelo diabetes. Contudo, o potencial antioxidante varia entre as espécies, algumas tenho uma melhor performance na recuperação dos parâmetros reprodutivos do que outras.

Asunto(s)

Animales , Masculino , Testículo/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Índice Glucémico/efectos de los fármacos , Complicaciones de la Diabetes , Fitoterapia , Testículo/fisiología , Extractos Vegetales

RESUMEN

Oxidative stress plays a main role in the development of diabetes complications. The impairment of gonadal antioxidant potential and endocrine disturbance in diabetic males causes testicular damage and failure in sperm production. Plants have been widely used to control diabetes due their hypoglycemic and antioxidant potential, contributing towards the recovery of testicular function. Current study comprises a review of the literature on the main medicinal plants used in the recovery of testicular oxidative damage in animals with experimental diabetes. Eighteen plant species in the nineteen studies selected from the search strategy were evaluated. Plant extracts were evaluated according to their effects on blood glucose and insulin levels, antioxidant enzymes and oxidant levels, lipid peroxidation, total protein, testosterone levels, gonadosomatic index, diameter of seminiferous tubules, seminiferous epithelium height and integrity, number of germ cells at stage VII and apoptosis in the seminiferous epithelium, sperm production, motility, viability and morphology. After the analysis of the studies, it was observed that plant species, used alone or in combination, may control testicular oxidative damage triggered by diabetes. The antioxidant potential varies among species, with some plants proving to have a better performance in the recovery of reproduction parameters than others.(AU)

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O estresse oxidativo desempenha um dos principais papéis no desenvolvimento das complicações do diabetes. Em machos diabéticos, as alterações ao potencial antioxidante gonadal e os distúrbios endócrinos resultam em danos testiculares e falhas na produção de espermatozoides. Plantas têm sido amplamente utilizadas como um método de controle do diabetes devido aos seus potenciais hipoglicemiante e antioxidante, contribuindo para a recuperação da função testicular. Assim, o objetivo deste estudo é fazer uma revisão da literatura sobre as principais plantas medicinais utilizadas na recuperação de danos oxidativos testiculares em animais induzidos ao diabetes experimental. Nos dezenove estudos selecionados a partir da estratégia de busca, dezoito espécies de plantas foram utilizadas. Os extratos das espécies vegetais foram avaliados de acordo com os seus efeitos nas concentrações de glicose e insulina, níveis das enzimas antioxidantes e dos oxidantes, peroxidação lipídica, quantidade de proteínas totais, níveis de testosterona, índice gonadossomático, diâmetro dos túbulos seminíferos, altura e integridade do epitélio seminífero, número de células germinativas no estágio VII, número de células em apoptose no epitélio seminífero, produção, motilidade, viabilidade e morfologia espermática. Depois das análises aos estudos, foi observado que as espécies vegetais, sozinhas ou em combinação, podem ajudar a controlar os danos oxidativos testiculares desencadeados pelo diabetes. Contudo, o potencial antioxidante varia entre as espécies, algumas tenho uma melhor performance na recuperação dos parâmetros reprodutivos do que outras.(AU)

Asunto(s)

Fitoterapia , Extractos Vegetales/uso terapéutico , Complicaciones de la Diabetes/terapia , Índice Glucémico , Estrés Oxidativo , Espermatogénesis

RESUMEN

Quality and sensory attributes of meat from 32 mixed-breed Santa Inês lambs fed diets composed of four silages with old man saltbush (Atriplex nummularia Lind), buffelgrass (Cenchrus ciliaris), Gliricidia (Gliricidia sepium), and Pornunça (Manihot sp.) were evaluated. Meat from lambs fed diet containing old man saltbush silage (P<0.05) showed greater values for cooking loss. Of the sensory attributes evaluated in the Longissimus lumborum muscle of the lambs, color and juiciness did not differ (P>0.05). However, the silages led to differences (P<0.05) in aroma, tenderness, and flavor values. The meat from animals fed the pornunça and Gliricidia silages was tenderer. Flavor scores were higher in meat from lambs that consumed old man saltbush silage and lower in the meat from those fed buffelgrass silage. Diets formulated with buffelgrass silage for sheep reduce meat production. Based on the results for carcass weight and meat quality, old man saltbush and pornunça are better silages for finishing sheep.

Asunto(s)

Dieta/veterinaria , Carne Roja/análisis , Ensilaje/análisis , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Cruzamiento , Fenómenos Químicos , Comportamiento del Consumidor , Proteínas en la Dieta/análisis , Estudios de Evaluación como Asunto , Fabaceae , Calidad de los Alimentos , Humanos , Masculino , Músculo Esquelético/química , Oveja Doméstica , Gusto

RESUMEN

ABSTRACT This study aimed to evaluate the influence of diets containing silage of different sorghum cultivars on the physical and chemical characteristics in the meat of lambs. Thirty five animals of undefined breed with average age between 5 and 7 months and average body weight of 17. 7±3.7kg were used. The lambs were randomly assigned to five treatments (sorghum silage BRS 610, BRS 655, BRS 800, BRS 810 e Ponta Negra) with seven replicates. When they reach 26.24kg of weight, the animals were sent to be slaughtered. Proceeded to the analysis of pH, color, cooking losses, shear force, determination of humidity, protein, ash and total amount of lipids in the Longissimus dorsi. The diets did not influence the parameters of pH (5.64), color (L*23.13, a* 18.00, b*15.78), cooking losses (34.43%), humidity (72.53%), protein (24.08%), ash (1.09%) or lipids (2.99%). The shear force differed among the diets, with the silage of cultivar BRS 655 providing a tender meat (2.10kgf/cm2). The sorghum silages used in in the diets did not interfere in the meat quality characteristics of feedlot lambs. Diets containing sorghum grain silage BRS 655 provided a tender meat.

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RESUMO Objetivou-se avaliar a influência de dietas contendo silagem de diferentes cultivares de sorgo sobre as características físicas e químicas na carne de ovinos sem padrão racial definido, com idade entre cinco e sete meses e peso vivo médio de 17,7±3,7kg, alimentados com dietas contendo silagem de diferentes cultivares de sorgo. O experimento foi conduzido na Estação Experimental Pendência, pertencente à Empresa Estadual de Pesquisa Agropecuária - Emepa, tendo um período experimental de 42 dias. Utilizou-se um delineamento inteiramente ao acaso, com cinco tratamentos (silagem de sorgo BRS 610, BRS 655, BRS 800, BRS 810 e Ponta Negra) e sete repetições. Ao atingirem o peso de abate de 26,24kg, os animais foram encaminhados ao abate. Procedeu-se à análise do pH, da cor, da perda de peso por cocção, da força de cisalhamento, bem como à determinação de umidade, proteínas, cinzas e lipídeos totais no músculo Longissimus dorsi. As dietas não influenciaram os parâmetros de pH (5,64), cor (L*23,13, a* 18,00, b*15,78), perdas de peso por cocção (34,43%), umidade (72,53%), proteína (24,08%), cinzas (1,09%) e lipídeos (2,99%). A força de cisalhamento diferiu entre as dietas, com a silagem do cultivar BRS 655 propiciando uma carne mais macia (2,10kgf/cm2). As silagens de sorgo utilizadas na confecção das dietas de cordeiros em confinamento não interferem nas características de qualidade da carne de ovinos sem padrão racial definido. Dietas contendo silagem de sorgo granífero BRS 655 proporcionaram uma carne mais macia.

RESUMEN

Some studies of polymorphisms in prostate cancer (PCa) analyze individuals in a uniform manner, regardless of genetic ancestry. However, PCa aggressiveness differs between subjects of African descent and those of European extraction. Thus, genetic ancestry analysis may be used to detect population stratification in case-control association studies. We genotyped 11 ancestry informative markers to estimate the contributions of African, European, and Amerindian ancestries in a case-control sample of 213 individuals from Bahia State, Northeast Brazil, including 104 PCa patients. We compared this data with self-reported ancestry and the stratification of cases by PCa aggressiveness according to Gleason score. A larger African genetic contribution (44%) was detected among cases, and a greater European contribution (61%) among controls. Self-declaration data revealed that 74% of PCa patients considered themselves non-white (black and brown), and 41.3% of controls viewed themselves as white. Our data showed a higher degree of European ancestry among fast-growing cancer cases than those of intermediate and slow development. This differs from many previous studies, in which the prevalence of African ancestry has been reported for all grades. Differences were observed between degrees of PCa aggressiveness in terms of genetic ancestry. In particular, the greater European contribution among patients with high-grade PCa indicates that a population's genetic structure can influence case-control studies. This investigation contributes to our understanding of the genetic basis of tumor aggressiveness among groups of different genetic ancestries, especially admixed populations, and has significant implications for the assessment of inter-population heterogeneity in drug treatment effects.

Asunto(s)

Población Negra/genética , Etnicidad/genética , Genoma Humano , Neoplasias de la Próstata/genética , Anciano , Brasil , Estudios de Casos y Controles , Frecuencia de los Genes/genética , Humanos , Masculino , Persona de Mediana Edad , Clasificación del Tumor , Pigmentación/genética , Población Blanca/genética

RESUMEN

Undernutrition represents a major public health challenge for middle- and low-income countries. This study aimed to evaluate whether a multideficient Northeast Brazil regional basic diet (RBD) induces acute morphological and functional changes in the ileum of mice. Swiss mice (∼25 g) were allocated into two groups: i) control mice were fed a standard diet and II) undernourished mice were fed the RBD. After 7 days, mice were killed and the ileum collected for evaluation of electrophysiological parameters (Ussing chambers), transcription (RT-qPCR) and protein expression (western blotting) of intestinal transporters and tight junctions. Body weight gain was significantly decreased in the undernourished group, which also showed decreased crypt depth but no alterations in villus height. Electrophysiology measurements showed a reduced basal short circuit current (Isc) in the undernourished group, with no differences in transepithelial resistance. Specific substrate-evoked Isc related to affinity and efficacy (glutamine and alanyl-glutamine) were not different between groups, except for the maximum Isc (efficacy) induced by glucose. Transcription of Sglt1 and Pept1 was significantly higher in the undernourished group, while SN-2 transcription was decreased. No changes were found in transcription of CAT-1 and CFTR, while claudin-2 and occludin transcriptions were significantly increased in the undernourished group. Despite mRNA changes, SGLT-1, PEPT-1, claudin-2 and occludin protein expression showed no difference between groups. These results demonstrate early effects of the RBD on mice, which include reduced body weight and crypt depth in the absence of significant alterations to villus morphology, intestinal transporters and tight junction expression.

Asunto(s)

Fenómenos Fisiológicos Nutricionales de los Animales , Crecimiento/fisiología , Íleon/anatomía & histología , Íleon/metabolismo , Desnutrición/metabolismo , Desnutrición/fisiopatología , Enfermedad Aguda , Animales , Peso Corporal , Modelos Animales de Enfermedad , Ingestión de Energía/fisiología , Immunoblotting , Absorción Intestinal/fisiología , Transporte Iónico/fisiología , Masculino , Desnutrición/complicaciones , Proteínas de Transporte de Membrana/análisis , Ratones , ARN Mensajero , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Proteínas de Uniones Estrechas/análisis , Proteínas de Uniones Estrechas/metabolismo , Factores de Tiempo