RESUMEN
The search for a more efficient repair process of muscle injuries has become evident in clinical practice. The aim of the present study was to evaluate the effect of nandrolone decanoate (ND) on the proliferation, adhesion, and expression of myogenic regulatory factors (MRFs) in C2C12 cells.Methods. Cell proliferation and adhesion were assessed using an MTT assay. The expression of MRFs was assessed by real-time PCR.Results. ND applied at 10 or 25 µM concentration induced after 60 min an increase in adhesion, at 5 µM concentration induced after 5 days an increase in cell proliferation, and ND at 50 µM concentration led after 5 days to a decrease in cell proliferation in comparison with other groups. The steroid did not alter the expression of MRFs.Conclusions. The positive effects of ND regarding the proliferation and adhesion of C2C12 cells suggest that this steroid may have positive effects following a muscle injury.
Asunto(s)
Proliferación Celular/efectos de los fármacos , Mioblastos/efectos de los fármacos , Nandrolona/análogos & derivados , Animales , Adhesión Celular/efectos de los fármacos , Adhesión Celular/genética , Proliferación Celular/genética , Células Cultivadas , Regulación de la Expresión Génica/efectos de los fármacos , Ratones , Desarrollo de Músculos/efectos de los fármacos , Desarrollo de Músculos/genética , Proteína MioD/genética , Proteína MioD/metabolismo , Mioblastos/fisiología , Miogenina/genética , Miogenina/metabolismo , Nandrolona/farmacología , Nandrolona Decanoato , Factores de Transcripción/genética , Factores de Transcripción/metabolismoRESUMEN
Four phytopathogenic fungi were cultivated up to six days in media containing chitooligosaccharide mixtures differing in average DP and FA. The three different mixtures were named Q3 (which contained oligosaccharides of DP2-DP10, with DP2-DP7 as main components), Q2 (which contained oligosaccharides of DP2-DP12, with DP2-DP10 as main components) and Q1 (which derived from Q2 and contained oligomers of DP5-DP8 with hexamer and a heptamer as the main components). The novel aspect of this work is the description of the effect of mixtures of oligosaccharides with different and known composition on fungal growth rates. The growth rate of Alternaria alternata and Rhizopus stolonifer was initially inhibited by Q3 and Q2 at higher concentrations. Q1 had a growth stimulating effect on these two fungi. Growth of Botrytis cinerea was inhibited by Q3 and Q2, while Q1 had no effect on the growth of this fungus. Growth of Penicillium expansum was only slightly inhibited by higher concentrations of sample Q3, while Q2 and Q1 had no effect. The inhibition of growth rates or their resistance toward chitooligosaccharides correlated with the absence or presence of chitinolytic enzymes in the culture media, respectively.
Asunto(s)
Alternaria/crecimiento & desarrollo , Botrytis/crecimiento & desarrollo , Quitina/metabolismo , Oligosacáridos/metabolismo , Penicillium/crecimiento & desarrollo , Enfermedades de las Plantas/microbiología , Rhizopus/crecimiento & desarrollo , Acetilación , Alternaria/enzimología , Botrytis/enzimología , Quitina/análogos & derivados , Medios de Cultivo , Frutas/microbiología , Cinética , Espectrometría de Masas , Oligosacáridos/química , Penicillium/enzimología , Rhizopus/enzimologíaRESUMEN
OBJECTIVES: To review procedures currently practiced in a Brazilian general hospital and to eliminate ineffective and inefficient practices. To measure the resulting cost improvements based on rigid hospital financing control. DESIGN: Implementation of surveillance and control programs and prevalence surveys to detect ineffective and inefficient practices. PARTICIPANTS: The study institution is a 130-bed general care facility affiliated with the Brazilian federal government. There were approximately 4,600 admissions per year during the study period (1986-1989). RESULTS: Instituting infection control measures and eliminating ineffective practices resulted in the following: an overall decrease in wound infection rates from 24.4% in 1987 to 3.45% in 1989; a 71% reduction in the global incidence of infection in the intensive care unit; a 74% reduction in the surgical prophylactic use of antibiotics; and a total savings of approximately $2 million (US dollars). CONCLUSIONS: During the period from 1986 to 1989, the infection control committee was able to decrease the overall wound infection rate from 24.4% in 1987 to 3.45% in 1989. This eliminated special health problems and improved patient care and cost-effectiveness for our hospital.