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Green extraction techniques (GreETs) emerged in the last decade as greener and sustainable alternatives to classical sample preparation procedures aiming to improve the selectivity and sensitivity of analytical methods, simultaneously reducing the deleterious side effects of classical extraction techniques (CETs) for both the operator and the environment. The implementation of improved processes that overcome the main constraints of classical methods in terms of efficiency and ability to minimize or eliminate the use and generation of harmful substances will promote more efficient use of energy and resources in close association with the principles supporting the concept of green chemistry. The current review aims to update the state of the art of some cutting-edge GreETs developed and implemented in recent years focusing on the improvement of the main analytical features, practical aspects, and relevant applications in the biological, food, and environmental fields. Approaches to improve and accelerate the extraction efficiency and to lower solvent consumption, including sorbent-based techniques, such as solid-phase microextraction (SPME) and fabric-phase sorbent extraction (FPSE), and solvent-based techniques (µQuEChERS; micro quick, easy, cheap, effective, rugged, and safe), ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE), in addition to supercritical fluid extraction (SFE) and pressurized solvent extraction (PSE), are highlighted.

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RESUMEN

Α novel, green, and facile fabric phase sorptive extraction (FPSE) prior to high pressure liquid chromatography with diode array detection (HPLC-DAD) methodology was developed for the efficient extraction and quantitative determination of tocopherols (α-, sum of (ß+γ), and δ-) in edible oils. Among several highly hydrophobic FPSE membranes, sol-gel polycaprolactone-polydimethylsiloxane-polycaprolactone (sol-gel PCAP-PDMS-PCAP) coated polyester FPSE membrane was found as the most efficient in extracting tocopherol homologues from edible oil samples. To maximize the extraction efficiency of FPSE membrane, major parameters of FPSE including the membrane size, sample loading time, the choice of the appropriate elution solvent and the elution solvent volume, desorption time, and the influence of stirring were systematically optimized. The developed FPSE-HPLC-DAD methodology was validated and presented adequately low limits of detection (LODs) and limits of quantification (LOQs) over the ranges 0.05-0.10 µg/g, and 0.17-0.33 µg/g, respectively. The RSD% of the within-day and between-day assays were lower than 1.3, and 11.8, respectively, demonstrating good method precision. The trueness of the method was assessed by means of relative percentage of recovery and ranged between 90.8 and 95.1% for within-day assay, and between 88.7-92.8% for between-day assay. The developed methodology was applied in the analysis of edible oils.

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The present pandemic has posed a crisis to the economy of the world and the health sector. Therefore, the race to expand research to understand some good molecular targets for vaccine and therapeutic development for SARS-CoV-2 is inevitable. The newly discovered coronavirus 2019 (COVID-19) is a positive sense, single-stranded RNA, and enveloped virus, assigned to the beta CoV genus. The virus (SARS-CoV-2) is more infectious than the previously detected coronaviruses (MERS and SARS). Findings from many studies have revealed that S protein and RdRp are good targets for drug repositioning, novel therapeutic development (antibodies and small molecule drugs), and vaccine discovery. Therapeutics such as chloroquine, convalescent plasma, monoclonal antibodies, spike binding peptides, and small molecules could alter the ability of S protein to bind to the ACE-2 receptor, and drugs such as remdesivir (targeting SARS-CoV-2 RdRp), favipir, and emetine could prevent SASR-CoV-2 RNA synthesis. The novel vaccines such as mRNA1273 (Moderna), 3LNP-mRNAs (Pfizer/BioNTech), and ChAdOx1-S (University of Oxford/Astra Zeneca) targeting S protein have proven to be effective in combating the present pandemic. Further exploration of the potential of S protein and RdRp is crucial in fighting the present pandemic.