RESUMEN
Ribosome biogenesis is initiated by RNA polymerase I (Pol I)-mediated synthesis of pre-ribosomal RNA (pre-rRNA). Pol I activity was previously linked to longevity, but the underlying mechanisms were not studied beyond effects on nucleolar structure and protein translation. Here we use multi-omics and functional tests to show that curtailment of Pol I activity remodels the lipidome and preserves mitochondrial function to promote longevity in Caenorhabditis elegans. Reduced pre-rRNA synthesis improves energy homeostasis and metabolic plasticity also in human primary cells. Conversely, the enhancement of pre-rRNA synthesis boosts growth and neuromuscular performance of young nematodes at the cost of accelerated metabolic decline, mitochondrial stress and premature aging. Moreover, restriction of Pol I activity extends lifespan more potently than direct repression of protein synthesis, and confers geroprotection even when initiated late in life, showcasing this intervention as an effective longevity and metabolic health treatment not limited by aging.
Asunto(s)
Proteínas de Caenorhabditis elegans , Longevidad , Animales , Humanos , Longevidad/genética , Caenorhabditis elegans/metabolismo , Proteínas de Caenorhabditis elegans/metabolismo , Precursores del ARN/metabolismo , Envejecimiento/genéticaRESUMEN
The need for a cure against HIV infection and a need to improve HIV screening amongst low risk population such as pregnant women have been an issue since the emergence of HIV. Therefore, a hospital-based cross-sectional study was adopted to determine the prevalence of antibodies to HIV-1/2/O and P24 antigen among pregnant women in Port Harcourt, Nigeria. Estimating the HIV seropositivity in pregnant women will offer vital information for monitoring the trend of HIV infection in the general population and assist in prevention from mother-to-child transmission. The occurrence of antibodies to HIV type 1/2/O and P24 antigen among the pregnant women was investigated using fourth-generation ELISA. The influence of the women's age, marital status, occupation, educational status and gestation period on the prevalence of these antibodies against HIV type 1/2/O and P24 antigen was also considered. Of the 86 pregnant women, 14 were found to be HIV-positive with seropositivity rate of 32.6%. Majority of HIV seropositive women (41.2%) were in the age group of 31-42 years. Higher seropositivity was observed among singles (50.0%), those with secondary education (44.7%), unemployed (47.8%) and in those in their third trimester (40.0%). Additionally, this study observed high seropositivity of antibodies to HIV type 1, 2, O and P24 antigen among pregnant women in Port Harcourt, Nigeria and calls for urgent and concerted efforts aimed at promoting behavioral and socio-cultural practices that could change the current high rate of HIV seropositivity amongst pregnant women in Nigeria. A combination of preventive strategies such as the use of condoms, breaking the chain of transmission within sexual networks by prompt treatment and reducing the amount of unsafe sexual behavior, promoting sexual abstinence and behavioral change from high-risk behavior are advocated.