RESUMEN

In collagenase isolated rat pancreatic islets, CCK-PZ, SHG, secretin and glucagon stimulated the accumulation of cAMP, in physiological ranges. The resulting increment of cAMP showed a good correlation with insulin release stimulated by either glucagon or secretin, but not by SHG or CCK-PZ. In the same system, 14CO2 production from glucose-U-14C was significantly increased by either SHG or CCK-PZ. The results presented in this report are compatible with the hypothesis that insulin release by gastrointestinal hormones may be mediated by cAMP in the B-cell in the case of either glucagon or secretin; whereas, in the case of either SHG or CCK-PZ, it may presumably be mediated by an unknown mechanism in glucose metabolism, other than c-AMP.

Asunto(s)

AMP Cíclico/metabolismo , Hormonas Gastrointestinales/farmacología , Insulina/metabolismo , Islotes Pancreáticos/metabolismo , Animales , Colecistoquinina/farmacología , Glucagón/farmacología , Secreción de Insulina , Masculino , Ratas , Secretina/farmacología