RESUMEN
This study was conducted to correlate clinical, laboratory, and bone marrow (BM) changes in cats naturally infected with feline leukemia virus and their association with viral loads in blood and BM and proviral loads in BM. Cats were classified into five groups based on antigenemia, clinical and/or laboratory findings and viral/proviral loads, according to a prospective study: symptomatic progressive (GI); asymptomatic progressive (GII); regressive (GIII); unclassified (GIV); or healthy (GV). |Correlations between these five groups and viral/proviral loads were evaluated. High viral and proviral loads were detected in GI and GII and viral loads were significantly associated with laboratory signs. Proviral loads detected in BM were significantly lower in GIII and GIV. GI cats were more likely to develop hematopoietic disorders than those from the other groups. Hematological and clinical disorders and disease severity are related to higher viral blood and proviral BM loads.
RESUMEN
Background: Canine distemper has been classified as highly contagious for most of domestic and wild carnivores, and theinfection can be fatal. Canine distemper inclusion bodies, also denominated Lenz inclusion bodies, are large aggregatesof viral nucleocapsid particles that can be form in red blood cells (RBCs), white blood cells (WBCs) and epithelial cellsin many tissues during the acute phase of infection. Their presence in blood is transient and rarely encountered in lightmicroscopy but are pathognomonic when identified in blood smears. The objective of this study was to investigate thefrequency of distemper inclusions in erythrocytes according to the fraction of the sample used for blood smears.Materials, Methods & Results: The study was conducted with routine blood sample provided by the Veterinary Laboratory of Clinical Analysis from the Veterinary Teaching Hospital of Universidade Federal do Rio Grande do Sul. TheEDTA-K2 blood sample of a 40 days old male dog, mixed breed, no immunization records, presenting diarrhea, hyporexia,myoclonus and pustules in the abdomen, was selected. In a routine peripheral blood smear examination, several distemperinclusions were observed in the erythrocytes. From this sample, ten smears were performed using a whole blood (WB)and top erythrocyte fraction combined with buffy coat, denominated of expanded buffy coat (EBC). The EBC fraction wasobtained after centrifugation of EDTA whole blood in microhematocrit tubes at 9600 g for 5 min to obtained the packedcell volume (PCV) and buffy coat. After centrifugation, the blood cells are separated into three layers based on density:platelets (adjacent to supernatant), WBCs, and RBCs in the bottom. The PCV was measured and the microhematocrit tubewas ruptured 2% below the interface between leukocytes and plasma, deposited into a plastic microtubes, homogenized andused for blood smear preparation. All smears were stained with Diff-Quick Stain...(AU)
Asunto(s)
Animales , Masculino , Perros , Moquillo/sangre , Moquillo/virología , Cuerpos de Inclusión Viral , Eritrocitos , Centrifugación/veterinariaRESUMEN
Background: Canine distemper has been classified as highly contagious for most of domestic and wild carnivores, and theinfection can be fatal. Canine distemper inclusion bodies, also denominated Lenz inclusion bodies, are large aggregatesof viral nucleocapsid particles that can be form in red blood cells (RBCs), white blood cells (WBCs) and epithelial cellsin many tissues during the acute phase of infection. Their presence in blood is transient and rarely encountered in lightmicroscopy but are pathognomonic when identified in blood smears. The objective of this study was to investigate thefrequency of distemper inclusions in erythrocytes according to the fraction of the sample used for blood smears.Materials, Methods & Results: The study was conducted with routine blood sample provided by the Veterinary Laboratory of Clinical Analysis from the Veterinary Teaching Hospital of Universidade Federal do Rio Grande do Sul. TheEDTA-K2 blood sample of a 40 days old male dog, mixed breed, no immunization records, presenting diarrhea, hyporexia,myoclonus and pustules in the abdomen, was selected. In a routine peripheral blood smear examination, several distemperinclusions were observed in the erythrocytes. From this sample, ten smears were performed using a whole blood (WB)and top erythrocyte fraction combined with buffy coat, denominated of expanded buffy coat (EBC). The EBC fraction wasobtained after centrifugation of EDTA whole blood in microhematocrit tubes at 9600 g for 5 min to obtained the packedcell volume (PCV) and buffy coat. After centrifugation, the blood cells are separated into three layers based on density:platelets (adjacent to supernatant), WBCs, and RBCs in the bottom. The PCV was measured and the microhematocrit tubewas ruptured 2% below the interface between leukocytes and plasma, deposited into a plastic microtubes, homogenized andused for blood smear preparation. All smears were stained with Diff-Quick Stain...