RESUMEN

Mucormycosis is a life-threatening infectious disease that occurs most commonly in immunocompromised patients such as those with hematological malignancies. Its clinical symptoms and associated radiological findings vary and specific biomarkers and culture characteristics have not been defined. An 85-year-old man who had been treated for myelodysplastic syndrome and tuberculosis for several months presented with subacute fever and worsening left-side chest pain. Contrast-enhanced computed tomography images depicted massive tumor-like consolidation without enhancement, expanding from the left lower lobe. Emboli that did not respond to anticoagulants were detected in the left descending pulmonary artery. Despite intensive treatment he developed multiple organ failure and died 47 days after hospitalization. Gross pathology of a lung autopsy specimen revealed left lower pulmonary arterial emboli and pulmonary infarction, which was concluded to be the direct cause of death. The emboli were histopathologically identified as invasive mycelia in vessels. Mucor sp. was detected via real-time polymerase chain reaction and immunohistopathological analyses revealed that the mold in the blood vessels of lung tissue was partially positive for the mucor antigen. In the present case of Mucor sp. pulmonary emboli in a patient with myelodysplastic syndrome, radiographic findings were hard to distinguish from those typical of a lung abscess.

RESUMEN

AIM: To examine the prevalence and detailed radiological findings of internal anomalies in thalidomide embryopathy (TE). MATERIALS AND METHODS: Whole-body image screening for internal anomalies using unenhanced whole-body computed tomography (CT) and head magnetic resonance imaging (MRI) was performed in 22 patients (13 women and nine men; mean age, 49 years; range, 47-51 years) with TE. RESULTS: Among the 22 patients with TE, internal anomalies were detected in 19 (86.4%): anomalies of the auditory organ in 10 (45.5%), anomalies of the vascular system in six (27.3%), agenesis of the gallbladder in six (27.3%), hypoplasia or aplasia of the 7th or 8th cranial nerves in five (22.7%), block vertebrae in five (22.7%), fusion of the left lobe and quadrate lobe of the liver in three (9.1%), and others in five (22.7%), respectively. CONCLUSION: In addition to limb defects or hypoplasia, various internal anomalies can be detected at a high incidence in TE using CT and MRI. Understanding these characteristic radiological findings may help radiologists detect a wide range of radiological findings of internal anomalies associated with TE.

Asunto(s)

Anomalías Múltiples/diagnóstico por imagen , Imagen por Resonancia Magnética/métodos , Tomografía Computarizada Multidetector/métodos , Efectos Tardíos de la Exposición Prenatal/diagnóstico por imagen , Talidomida/efectos adversos , Imagen de Cuerpo Entero/métodos , Anomalías Múltiples/epidemiología , Causalidad , Femenino , Vesícula Biliar/anomalías , Vesícula Biliar/diagnóstico por imagen , Vesícula Biliar/efectos de los fármacos , Humanos , Japón/epidemiología , Hígado/anomalías , Hígado/diagnóstico por imagen , Hígado/efectos de los fármacos , Masculino , Persona de Mediana Edad , Embarazo , Efectos Tardíos de la Exposición Prenatal/epidemiología , Prevalencia , Columna Vertebral/anomalías , Columna Vertebral/diagnóstico por imagen , Columna Vertebral/efectos de los fármacos

RESUMEN

Eph receptor tyrosine kinases and their ligands have been shown to be involved in processes of cell migration and axon guidance during embryonic development. Here we describe the development of a function-blocking monoclonal antibody against chick ephrin-A2, and its effect on retinal ganglion cell axons studied both in vitro and in vivo. In the stripe assay, the blocking antibody completely abolished the repulsive effect of posterior tectal membranes. In vivo, in a loss-of-function approach, hybridoma cells secreting the antiephrin-A2 antibody were applied to chick embryos from embryonic day 3 (E3) on, and the retinotectal projection was subsequently analyzed at E16. DiI tracing analyses showed that although the projection of both temporal and nasal retinal ganglion axons in the tectum was, overall, normal, occasionally diffuse and extra termination zones were observed, in addition to axons over-shooting their termination zones. These data support the idea that ephrin-A2 contributes to the establishment of the chick retinotectal projection.

Asunto(s)

Embrión de Pollo/fisiología , Retina/embriología , Colículos Superiores/embriología , Transmisión Sináptica/fisiología , Factores de Transcripción/metabolismo , Animales , Anticuerpos Monoclonales , Axones/fisiología , Efrina-A2 , Factores de Transcripción/fisiología

RESUMEN

We reported a 19-year-old woman with multiple sclerosis (MS) with recurrent episodes of psychiatric symptoms at the age of 10 and 19, as the first and 11th relapse, respectively. Her first episode of MS resembled acute encephalitis at the age of 9 and she relapsed 12 times. The psychiatric symptoms were not mood disorders but altered states of consciousness, such as the delirous state. She was treated with high doses of intravenous methylpredonisolone and her symptoms were almost resolved, but her anxiety neurosis remained. Her MRI showed demyelination in the white matter of the frontal lobe and the hippocampus, which suggested that psychiatric symptoms of MS would be related to the frontal lobe lesion.

Asunto(s)

Trastornos de la Conciencia/etiología , Delirio/etiología , Esclerosis Múltiple/psicología , Adulto , Femenino , Humanos , Esclerosis Múltiple/complicaciones , Recurrencia

RESUMEN

We developed a new diagnostic method of subgenus (Sub) B adenovirus (Ad) in clinical samples using non-nested polymerase chain reaction (PCR). Sequences of the conserved hexon-coding region of representative strains of eight serotypes (3, 7, 11, 14, 16, 21, 34 and 35) of Sub B Ad were heterogeneous. In order to distinguish Ad serotype 3 (Ad 3) and Ad 7 from the other serotypes of Sub B Ad, and to differentiate Ad 3 and 7 from each other, 3 different downstream primers were designed based on the sequence heterogeneity. By a single-tube PCR method using a combination of 6 primers including the 3 new primers, Ads demonstrated to amplify 188, 206, 284, and 301 bp DNA fragments for Ad 3, Ad 7, other Sub B Ads, and non-Sub B Ads, respectively. A total of 114 clinical samples were selected to evaluate the direct applicability of our PCR. The results were compared with previous culture results. Sixty-seven out of 71 (94%) Sub B Ad culture-positive samples, and 15 out of 19 (79%) Sub C or E-positive samples amplified products of the expected size. Two of 20 (10%) culture-negative samples from pharyngoconjunctival fever patients were identified as Ad 3 by the PCR. Four samples, from which non-Ad viruses were isolated, were negative by the PCR. The present study might provide a rapid and sensitive diagnosis method for infections caused by Sub B Ads.

Asunto(s)

Infecciones por Adenovirus Humanos/diagnóstico , Adenovirus Humanos/clasificación , Proteínas de la Cápside , Reacción en Cadena de la Polimerasa/métodos , Infecciones por Adenovirus Humanos/virología , Adenovirus Humanos/genética , Adenovirus Humanos/aislamiento & purificación , Secuencia de Bases , Cápside/genética , ADN Viral/análisis , ADN Viral/genética , Humanos , Datos de Secuencia Molecular , Nasofaringe/virología , Sensibilidad y Especificidad , Análisis de Secuencia de ADN , Serotipificación , Cultivo de Virus

RESUMEN

Otx2 is expressed in the mesencephalon and prosencephalon, and Gbx2 is expressed in the rhombencephalon around stage 10. Loss-of-function studies of these genes in mice have revealed that Otx2 is indispensable for the development of the anterior brain segment, and that Gbx2 is required for the development of the isthmus. We carried out gain-of-function experiments of these genes in chick embryos with a newly developed gene transfer system, in ovo electroporation. When Otx2 was ectopically expressed caudally beyond the midbrain-hindbrain boundary (MHB), the alar plate of the metencephalon differentiated into the optic tectum instead of differentiating into the cerebellum. On the other hand, when Gbx2 was ectopically expressed at the mesencephalon, the caudal limit of the tectum shifted rostrally. We looked at the effects of misexpression on the isthmus- and tectum-related molecules. Otx2 and Gbx2 interacted to repress each other's expression. Ectopic Otx2 and Gbx2 repressed endogenous expression of Fgf8 in the isthmus, but induced Fgf8 expression at the interface between Otx2 and Gbx2 expression. Thus, it is suggested that interaction between Otx2 and Gbx2 determines the site of Fgf8 expression and the posterior limit of the tectum.

Asunto(s)

Proteínas de Homeodominio/metabolismo , Proteínas del Tejido Nervioso/metabolismo , Colículos Superiores/embriología , Transactivadores/metabolismo , Animales , Embrión de Pollo , Factor 8 de Crecimiento de Fibroblastos , Factores de Crecimiento de Fibroblastos/genética , Regulación del Desarrollo de la Expresión Génica , Proteínas de Homeodominio/genética , Proteínas del Tejido Nervioso/genética , Factores de Transcripción Otx , Transactivadores/genética

RESUMEN

Transplantation experiments have shown that the mes-metencephalic boundary (isthmus) acts as an organizer for the development of the optic tectum. We have cloned Pax-2 which is expressed in the isthmus. Previously it was shown that Pax-5, a member of the same Pax subfamily as Pax-2, transformed the diencephalon into a tectum-like structure and induced isthmus- and tectum-related genes both in the mesencephalon and in the diencephalon. In order to define the distinct roles between Pax-2 and Pax-5 in development of the tectum, we expressed Pax-2 ectopically in the mesencephalon and the diencephalon of E2 chick embryos by in ovo electroporation. Histological observation demonstrated that Pax-2 transformed the diencephalon into a tectum-like structure. In Pax-2, transfected embryos the expression of isthmus- and tectum-related genes such as Fgf8 and En-2 was induced in the diencephalon. However, neither Fgf8 nor En-2 expression was induced in the mesencephalon, making a striking contrast with the result of Pax-5 misexpression. In E2 chick embryos, the mesencephalon is committed of its fate to differentiate into the tectum, but the diencephalon has plasticity on its fate. Moreover, Pax-2 expression in the isthmus precedes Pax-5 expression. Taking these results into consideration, it is suggested that Pax-2 plays a crucial role in initiation of the tectal development, and that Pax-5 functions to maintain the state of tectal differentiation.

Asunto(s)

Embrión de Pollo/metabolismo , Proteínas de Unión al ADN/genética , Regulación del Desarrollo de la Expresión Génica/fisiología , Colículos Superiores/metabolismo , Factores de Transcripción/genética , Secuencia de Aminoácidos , Animales , Embrión de Pollo/crecimiento & desarrollo , Clonación Molecular , Diencéfalo/embriología , Diencéfalo/metabolismo , Mesencéfalo/embriología , Mesencéfalo/metabolismo , Datos de Secuencia Molecular , Factor de Transcripción PAX2 , Homología de Secuencia de Aminoácido , Colículos Superiores/embriología , Transformación Genética

RESUMEN

The mes-metencephalic boundary (isthmus) has been suggested to act as an organizer in the development of the optic tectum. Pax-5 was cloned as a candidate for regulator of the organizing center. Isthmus-specific expression of Pax-5 and analogy with the genetic cascade in Drosophila suggest that Pax-5 may be at a higher hierarchical position in the gene regulation cascade of tectum development. To examine this possibility, a gain-of-function experiment on Pax-5 was carried out. In ovo electroporation on E2 chick brain with the eucaryotic expression vector that encodes chick Pax-5 cDNA was used. Not only was a considerable amount of Pax-5 expressed ectopically in the transfected brain, but irregular bulging of the neuroepithelium was induced in the diencephalon and mesencephalon. At Pax-5 misexpressing sites, uptake of BrdU was increased. Histological examination of E7 transfected brain revealed that Pax-5 caused transdifferentiation of diencephalon into the tectum-like structure. In the bulges of the E7 mesencephalon, differentiation of laminar structure was repressed when compared to the normal side. In transfected embryos, En-2, Wnt-1 and Fgf8 were up-regulated ectopically, and Otx2 was down-regulated in the diencephalon to mesencephalon. Moreover, Ephrin-A2, which is expressed specifically in the tectum with a gradient highest at the caudal end, is suggested to be involved in pathfinding of the retinal fibers, and was induced in the bulges. When the mouse Fgf8 expression vector was electroporated, Pax-5 and chick Fgf8 were also induced ectopically. These results suggest that Pax-5, together with Fgf8, hold a higher position in the genetic hierarchy of the isthmus organizing center and regulate its activity.

Asunto(s)

Proteínas de Unión al ADN/metabolismo , Inducción Embrionaria , Proteínas Nucleares/metabolismo , Rombencéfalo/embriología , Colículos Superiores/embriología , Factores de Transcripción , Proteínas de Pez Cebra , Secuencia de Aminoácidos , Animales , Diferenciación Celular , Embrión de Pollo , Clonación Molecular , ADN Complementario , Proteínas de Unión al ADN/genética , Electroporación/instrumentación , Factor 8 de Crecimiento de Fibroblastos , Factores de Crecimiento de Fibroblastos/metabolismo , Regulación del Desarrollo de la Expresión Génica , Proteínas de Homeodominio/metabolismo , Mesencéfalo/embriología , Ratones , Datos de Secuencia Molecular , Proteínas del Tejido Nervioso/metabolismo , Proteínas Nucleares/genética , Factores de Transcripción Otx , Factor de Transcripción PAX5 , Puente/embriología , Proteínas Proto-Oncogénicas/metabolismo , Homología de Secuencia de Aminoácido , Distribución Tisular , Transactivadores/metabolismo , Proteínas Wnt , Proteína Wnt1

RESUMEN

The optic tectum receives axons from the retinal ganglion cells in a topographical ordered manner. For the precise retinotectal projection, retinal and tectal compartments must be defined first, and then retinal and tectal cells are positionally specified. In chick tectal primordia, a homeobox-containing gene En, a homologue of a Drosophila segment polarity gene engrailed, is expressed in a caudal-to-rostral gradient, and transplantation and misexpression experiments have demonstrated that En confers posterior characteristics by controlling Ephrin A2 and Ephrin A5 expression. On the other hand, it has been shown that the mesmetencephalic boundary (isthmus) acts as an organizer for the tectum. We focused on paired box-containing genes Pax-2 and Pax-5, which are expressed in the isthmus. Comparison of the expression of Pax-2 and Pax-5 in the isthmic region and the results of overexpression of Pax-2 and Pax-5 in the mesencephalon and in the diencephalon suggested that Pax-2 plays a crucial role in establishment of the tectal region and that Pax-5 functions to maintain the state of tectal differentiation and rostrocaudal polarity of the tectum.

Asunto(s)

Tipificación del Cuerpo/genética , Colículos Superiores/embriología , Animales , Embrión de Pollo , Regulación del Desarrollo de la Expresión Génica , Genes/fisiología

Asunto(s)

Exantema Súbito/complicaciones , Síndrome de Secreción Inadecuada de ADH/complicaciones , Exantema Súbito/diagnóstico , Exantema Súbito/terapia , Humanos , Síndrome de Secreción Inadecuada de ADH/diagnóstico , Síndrome de Secreción Inadecuada de ADH/terapia , Lactante , Masculino

RESUMEN

Annexin VII is believed to be required for proper Ca(2+)-homeostasis in Dictyostelium discoideum cells. As was previously reported, the expression of Annexin VII gene increased during the transition of D. discoideum Ax-2 cells from growth to differentiation. We have casually cloned an interesting gene, Quit3, by the differential plaque hybridization. Quit3 had no coding region, and was expressed more predominantly in the growth phase than in the differentiation phase. Unexpectedly, this gene was found to encode the complementary sequence of Annexin VII. Therefore, it is most likely that the Quit3 mRNA may regulate the Annexin VII synthesis by the natural antisense transcript via an antisense RNA-RNA interaction, thus resulting in striking increase of Annexin VII production in the phase-shift of cells from growth to differentiation. Since Annexin VII is known to be coded for by a single gene in Dictyostelium, the antisense RNA seemed to be encoded in the same genetic locus as the Annexin VII mRNA.

Asunto(s)

Anexina A7/genética , Dictyostelium/crecimiento & desarrollo , Dictyostelium/genética , Regulación Fúngica de la Expresión Génica , ARN sin Sentido/fisiología , Secuencia de Aminoácidos , Animales , Anexina A7/biosíntesis , Secuencia de Bases , Diferenciación Celular/efectos de los fármacos , Diferenciación Celular/genética , División Celular/efectos de los fármacos , División Celular/genética , Clonación Molecular , ADN Complementario/aislamiento & purificación , Dictyostelium/citología , Proteínas Fúngicas/genética , Regulación Fúngica de la Expresión Génica/efectos de los fármacos , Genes Fúngicos , Datos de Secuencia Molecular

RESUMEN

To clarify the pathogenesis of the vascular injury caused by the administration of anti-neoplastic drugs, cisplatin with lipiodol was infused into the superior mesenteric artery of rats. Morphological and biochemical changes in the vascular wall and the prophylactic effects of vitamin E were examined 4 days after administration. In the cisplatin-treated group, but not in the other groups, severe endothelial injury, such as vacuolation, subendothelial edema, and destruction of the internal elastic membrane, was observed. Superoxide dismutase, a potent scavenger of the superoxide anion, was markedly lower in the cisplatin group, and Na/K-ATPase, a marker of the plasma membrane, was also low in this group. These morphological changes were minimal, and enzyme activity was higher in the vitamin E-treated group than in the cisplatin-treated group. These findings indicate that endothelial injury after cisplatin administration could be caused by free radical-induced lipid peroxidation of the membrane system, and that such injury may be prevented by the co-administration of vitamin E.

Asunto(s)

Cisplatino/toxicidad , Endotelio Vascular/efectos de los fármacos , Arteria Mesentérica Superior/efectos de los fármacos , Especies Reactivas de Oxígeno/metabolismo , Vitamina E/farmacología , 5'-Nucleotidasa/metabolismo , Animales , Endotelio Vascular/patología , Radicales Libres , Peroxidación de Lípido/efectos de los fármacos , Masculino , Arteria Mesentérica Superior/patología , Ratas , Ratas Wistar , ATPasa Intercambiadora de Sodio-Potasio/metabolismo , Superóxido Dismutasa/metabolismo

RESUMEN

In order to elucidate the role of protein C (PC) in the rat, we expressed, purified, and characterized recombinant rat PC. The purified recombinant rat PC was 70-90% two-chain (41 kDa heavy chain; 22 and 23 kDa light chain) and 10-30% single-chain (61 kDa). Amino acid analysis confirmed the presence of 10 moles of gamma-carboxyglutamic acid residues per mol of protein. For comparison, plasma rat PC was purified from a barium citrate precipitate using similar method. Plasma rat PC was a two-chain form (41 kDa heavy chain; 22 kDa light chain) with no detectable single-chain nor 23 kDa light chain. For determination of the in vitro secreted species, primary cultured rat hepatocytes were incubated for 6 h with methionine-free MEM containing vitamin K1, aprotinin, and [35S]methionine. The supernatant was immunoprecipitated and analyzed by SDS-PAGE followed by autoradiography. Approximately 90% of the PC radioactivity migrated as a two-chain molecule. These results indicate that rat PC is secreted mainly as a two-chain molecule from the liver. PROTAC-activated forms of recombinant rat PC, plasma rat PC, and plasma human PC hydrolyzed the S-2366 chromogenic substrate at the same rate. Recombinant rat PC was also activated by the thrombin-thrombomodulin complex at a rate similar to plasma rat PC. The anticoagulant activities of the three activated PCs were examined in rat plasma. Both recombinant and plasma rat PC prolonged the activated partial thromboplastin time in a dose-dependent manner, but plasma human PC was less effective.(ABSTRACT TRUNCATED AT 250 WORDS)

Asunto(s)

Proteína C/química , Proteínas Recombinantes de Fusión/química , Secuencia de Aminoácidos , Aminoácidos/análisis , Animales , Secuencia de Bases , Western Blotting , Células CHO , Células Cultivadas , Cricetinae , Hígado/citología , Masculino , Datos de Secuencia Molecular , Proteína C/biosíntesis , Proteína C/fisiología , Conformación Proteica , Ratas , Ratas Wistar , Proteínas Recombinantes de Fusión/biosíntesis

Asunto(s)

Cisplatino/administración & dosificación , Neoplasias Hepáticas Experimentales/tratamiento farmacológico , Animales , Portadores de Fármacos , Arteria Hepática , Inyecciones Intraarteriales , Liposomas , Ratas

RESUMEN

Enhancement of liver cancer on ultrasonography (US) by injection of new contrast medium (LEVOVIST) was performed in rats with 3'-methyl-4-dimethylaminoazobenzene-induced hepatic carcinoma. Echosignals of cancer nodules increased remarkably after the intrahepatic arterial injection of LEVOVIST (200, 300 mg/ml), and contrast enhancement was observed for at least 15 minutes. Furthermore, US after the intrahepatic arterial injection of LEVOVIST (200 mg/ml) can visualize small nodule, which was not recognizable on plain US. As a result of increased echosignals of normal liver parenchyma after the intra-portal injection of LEVOVIST (300 mg/ml), the cancer nodules were demonstrated as hypoechoic. These results indicate that this new contrast medium for sonography is effective in the diagnosis of liver cancer.

Asunto(s)

Medios de Contraste/normas , Aumento de la Imagen , Neoplasias Hepáticas Experimentales/diagnóstico por imagen , Animales , Evaluación de Medicamentos , Neoplasias Hepáticas Experimentales/inducido químicamente , Metildimetilaminoazobenceno , Ratas , Ultrasonografía

RESUMEN

We cloned a cDNA coding for rat protein C, which provides hybridization probes for the detection of protein C mRNA in several tissues. The cloned cDNA was 1543 bp long and contained a single open reading frame of 1383 nucleotides. The proposed rat protein C precursor contained 461 amino acid residues: a 41 amino acid preproleader sequence, and light (155 amino acids) and heavy (263 amino acids) chains joined by a Lys-Arg dipeptide. Northern blot analysis showed that the rat protein C mRNA was expressed not only in the liver, but also in the kidney.

Asunto(s)

Proteína C/genética , ARN Mensajero/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Northern Blotting , Clonación Molecular , Riñón/metabolismo , Datos de Secuencia Molecular , Sistemas de Lectura Abierta/genética , Proteína C/química , Precursores de Proteínas/genética , Ratas , Homología de Secuencia de Ácido Nucleico

RESUMEN

As part of a series of basic experiments on using metallic stents for treatment of vascular stenosis, a chronological examination of changes in dog aorta following implantation of a self-expandable metallic stent was conducting using transmission and scanning electron microscopy. The Giantruco stent was placed in the abdominal aorta via the right carotid artery. One week after insertion, the aortic intima was depressed and degenerative changes observed in both endothelial and medial smooth muscle cells. After 2 weeks, except for the bend portion, the stent was covered with neointima. The neointimal surface was covered by premature endothelial cells with abundant microvilli and prominent nuclear protrusions. Under the endothelial cells, immature mesenchymal cells, such as fibroblast, were scattered throughout the edematous intercellular space. At 4 weeks, the stent was completely covered by neointima and the endothelial cells had flattened and few microvilli were in evidence. In this thickened intima, premature smooth muscle cells with myofilaments and basement membranes were observed but, around them, few collagen fibers and only occasional elastic fibers were found. At 6 weeks, the intimal surfaces were flat and smooth with a slight intimal elevation over the stent. No thrombus was observed throughout the period of the experiment. The above results indicate that dilation using metallic stents may be a useful method for treatment of vascular stenosis.

Asunto(s)

Aorta Abdominal , Stents , Animales , Perros , Microscopía Electrónica , Microscopía Electrónica de Rastreo

RESUMEN

To assess the relationship between relatively severe hereditary hypercholesterolemia with Achilles tendon xanthomas and the defect of the low density lipoprotein (LDL) receptor gene, family studies were carried out in 17 hypercholesterolemic families. In 16 out of the 17 families, hypercholesterolemia co-segregated with four different gross rearrangements, six different restriction fragment length polymorphism (RFLP) haplotypes, or an abnormal TaqI band of the LDL receptor gene. These findings are compatible with the interpretation that hypercholesterolemia is caused by defective LDL receptor genes, and that the origin of the mutant LDL receptor genes in Japanese generally differs among different pedigrees. In the remaining family, the proband and his sibling, both having relatively severe hypercholesterolemia and Achilles tendon xanthomas, shared an RFLP haplotype, although the proband's other sibling with moderate hypercholesterolemia but without Achilles tendon xanthomas did not. The mutant gene for familial defective apolipoprotein B-100 was not detected in the 17 probands. These data suggest that most, if not all, of the relatively severe hereditary hypercholesterolemia associated with Achilles tendon xanthomas is caused by a defect of the LDL receptor gene.

Asunto(s)

Tendón Calcáneo , Hipercolesterolemia/genética , Receptores de LDL/genética , Xantomatosis/genética , Femenino , Humanos , Hipercolesterolemia/complicaciones , Desequilibrio de Ligamiento , Masculino , Enfermedades Musculares/complicaciones , Enfermedades Musculares/genética , Linaje , Polimorfismo de Longitud del Fragmento de Restricción , Xantomatosis/complicaciones

RESUMEN

The serial histological changes of the liver following the intrahepatic arterial injection of Lipiodol in the rat with cirrhosis were investigated. The hepatocytes showed acidophilic degeneration and focal necrosis after 12 hours and restoration of focal necrosis was seen after 72 hours. Necrosis and infarction were resolved after 120 hours. We concluded that the intrahepatic arterial injection of 0.1 ml Lipiodol in the rat with cirrhosis was safely performed.

Asunto(s)

Aceite Yodado/efectos adversos , Cirrosis Hepática Experimental/patología , Animales , Arteria Hepática , Inyecciones Intraarteriales , Aceite Yodado/administración & dosificación , Regeneración Hepática , Masculino , Necrosis , Ratas , Ratas Endogámicas

RESUMEN

A case of extramedullary plasmacytoma of the spleen in a 39 years-old man was reported. The tumor occupied almost of the spleen and extended to the transverse colon. 67Ga scintigraphy and MRI, particularly T 2-weighted image were useful in diagnosis of the localization.

Asunto(s)

Neoplasias del Colon/diagnóstico , Mieloma Múltiple/diagnóstico , Neoplasias del Bazo/diagnóstico , Adulto , Neoplasias del Colon/patología , Radioisótopos de Galio , Humanos , Imagen por Resonancia Magnética , Masculino , Mieloma Múltiple/patología , Arteria Esplénica/diagnóstico por imagen , Neoplasias del Bazo/patología , Tomografía Computarizada por Rayos X