RESUMEN
In the present study, the levels of SOD activity and Cu, Zn-SOD mRNA in the brain, kidney, liver and eye of normal and Upjohn Pharmaceutics Limited (UPL) rats, a new hereditary cataract model derived from Sprague-Dawley rats, were measured. Although the levels of SOD activity in the eye and brain of UPL rats were significantly decreased compared with those of normal rats 3 and 5 weeks after birth, the levels of SOD activities in the kidney and liver were the same in both groups. The levels of Cu, Zn-SOD mRNA in kidney and liver of UPL rats were the same as those of normal controls. The level of Cu, Zn-SOD mRNA in the brain of normal rats 5 weeks after birth was about twofold greater than that of UPL, and that in the eye of UPL rats 3 weeks after birth was significantly decreased compared with that of normal controls. The sequences of cDNA encoding Cu, Zn-SOD and the sequences of the regulatory region of the Cu, Zn-SOD gene were confirmed to be the same in normal and UPL rats. These results indicated that the decreases in levels of SOD activity and Cu, Zn-SOD mRNA in the brain and eye of UPL rat were not due to mutation of the genomic Cu, Zn-SOD gene in UPL rats or differences in the sequence of the regulatory region of the Cu, Zn-SOD gene between normal and UPL rats.
Asunto(s)
Catarata/enzimología , Superóxido Dismutasa/metabolismo , Animales , Secuencia de Bases , Encéfalo/enzimología , Catarata/genética , ADN Complementario/genética , Ojo/enzimología , Femenino , Riñón/enzimología , Hígado/enzimología , Masculino , Datos de Secuencia Molecular , ARN Mensajero/metabolismo , Ratas , Ratas Mutantes , Ratas Sprague-Dawley , Secuencias Reguladoras de Ácidos Nucleicos , Homología de Secuencia de Ácido Nucleico , Superóxido Dismutasa/genéticaRESUMEN
When most of the K+ in a chemically defined medium was replaced with Rb+, cell growth of HeLa cells was strongly inhibited. The growth was partially but significantly restored by an addition of 5% dialyzed calf serum to the medium. The inhibition of cell growth in Rb+-substituted medium was partly due to suppression of protein synthesis by K+ deficiency, but the key mechanism of inhibition is still unknown. Rb+ substitution did not influence protein degradation or nucleic acid synthesis. The restoration of cell growth on addition of serum took place chiefly through stimulation of DNA synthesis. Protein and RNA syntheses were not affected by addition of serum, and serum-induced prevention of protein degradation was less in Rb+-substituted medium than in normal K+ medium.