RESUMEN
The impact of low-dose-rate radiation on genetics is largely unknown, particularly in natural environments. The Fukushima Dai-ich Nuclear Power Plant disaster resulted in the creation of contaminated natural lands. In this study, de novo mutations (DNMs) in germ line cells were surveyed from double-digest RADseq fragments in Japanese cedar and flowering cherry trees exposed to ambient dose rates ranging from 0.08 to 6.86 µGy h-1. These two species are among the most widely cultivated Japanese gymnosperm and angiosperm trees for forestry and horticultural purpose, respectively. For Japanese flowering cherry, open crossings were performed to produce seedlings, and only two candidate DNMs were detected from uncontaminated area. For Japanese cedar, the haploid megagametophytes were used as next generation samples. The use of megagametophytes from open crossing for next generation mutation screening had many advantages such as reducing exposure to radiation in contaminated areas because artificial crossings are not needed and the ease of data analysis owing to the haploid nature of megagametophytes. A direct comparison of the nucleotide sequences of parents and megagametophytes revealed an average of 1.4 candidate DNMs per megagametophyte sample (range: 0-40) after filtering procedures were optimized based on the validation of DNMs via Sanger sequencing. There was no relationship between the observed mutations and the ambient dose rate in the growing area or the concentration of 137Cs in cedar branches. The present results also suggest that mutation rates differ among lineages and that the growing environment has a relatively large influence on these mutation rates. These results suggested there was no significant increase in the mutation rate of the germplasm of Japanese cedar and flowering cherry trees growing in the contaminated areas.
Asunto(s)
Desastres , Accidente Nuclear de Fukushima , Monitoreo de Radiación , Contaminantes Radiactivos del Suelo , Árboles/genética , Monitoreo de Radiación/métodos , Contaminantes Radiactivos del Suelo/análisis , Radioisótopos de Cesio/análisis , JapónRESUMEN
After the 2011 Fukushima Dai-ichi Nuclear Power Station (FDNPS) accident, wild populations of animals and plants living in the evacuation zone received additional ionizing radiation of both internal and external radiation doses. Morphological abnormalities of pine and fir trees near the FDNPS were reported. In order to evaluate dose-effect relationships, it is necessary to quantify the radiation doses to trees and plants. In this study, the internal and external dose rates to Japanese cedar and plants collected at three sites in Okuma, approximately 4 km southwest of FDNPS were estimated applying the ERICA Assessment Tool. The activity concentrations of 134Cs and 137Cs in soils, cedar trunks, and plants were determined. The total dose rates to cedar ranged from 2.2 ± 1.2 to 6.1 ± 2.2 µGy h-1. These rates were within the derived consideration reference levels (DCRLs) reported by ICRP 108 as 4-40 µGy h-1 for pine trees. The highest estimate for plants was 7.1 ± 2.7 µGy h-1, much smaller than the DCRLs reported for grasses and herbs (40-400 µGy h-1). On average, the internal radiation dose rates to cedars at the two sites accounted for 5% and 29% of the external dose rates, respectively, while the value in another site was only 0.4% for cedar. This was attributed to differences in the crown area between the three sites. The trunk diameter of cedars shows a positive correlation with the ratio of internal to external radiation dose rates. It indicates that the total dose rate to cedars is easily estimated with the soil radiocaesium inventory and trunk diameter. The internal radiation dose rate to the plant varied depending on the plant species. This variation was considerably large in plants due to the presence of two species, including Solidago altissima and Artemisia indica var. maximowiczii.