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OBJECTIVE: Oxidative stress has been suggested to have a pivotal role in the development of cardiovascular disease in kidney transplant patients (KTPs). The effects of fluvastatin on oxidative status in KTPs have not been well evaluated. The aim of the present study was to evaluate the effects of fluvastatin on oxidative status by investigating erythrocyte superoxide dismutase (SOD), erythrocyte glutathione peroxidase (GPx), serum paraoxonase (PON1), and serum arylesterase (ARE), along with lipid peroxidation products, serum malonldialdehyde, and apolipoprotein B malondialdehyde (ApoB MDA). METHODS: Eighteen KTPs were included in the present study. Blood samples were obtained after 1 night's fast. Erythrocyte SOD, erythrocyte GPx, serum PON1, serum ARE, serum MDA, and ApoB MDA were measured using methods described previously. Paired-sample t test was used for comparing the changes from week 0 to week 4 of parameters that might be associated with fluvastatin treatment. RESULTS: The present study has shown that erythrocyte SOD and GPx, and serum PON1 and ARE activities increased at the fourth week of the statin treatment. Furthermore an increase in the antioxidant enzymes following fluvastatin may be a clue for the antioxidant effects of this drug. Four weeks of fluvastatin long-acting tablets 80 mg/day led to a decrease in plasma Apo-MDA and MDA levels. CONCLUSION: The findings of the present study demonstrate that fluvastatin 80 mg long-acting tablets may be used safely for 4 weeks and decrease atherogenic lipoproteins in KTPs. Furthermore, after 4 weeks of fluvastatin treatment, the levels of antioxidant parameters increased and oxidative parameters decreased. Further placebo-controlled treatment studies would be helpful to evaluate the effects of fluvastatin on oxidant and antioxidant parameters including PON1 in patients with KT.

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This study was conducted to determine weights of body (BW), carcass (CW), gastrointestinal tract (GTW), meat quality and some blood metabolite responses to corn starch, saccharose or glucose administration in the drinking water during pre-slaughter feed withdrawal (FW) in broilers. On day 42 of age, 200 broilers (Ross 308) were allocated randomly to five treatments with four replicates. During a 10-h FW, control broilers (C) were provided with non-treated water and the standard finisher diet ad libitum, whereas fasted broilers provided with non-treated (NFW) or treated water, 3 g glucose (G), saccharose (S) or corn starch (CS)/L. Eight birds (four males and four females) per treatment were slaughtered. Birds receiving non-treated or treated water had lower BW and higher carcass yield than the full-fed broilers. The full-fed broilers had higher absolute and relative GTW than the fasted birds. Broilers consumed more readily treated water compared with non-treated water. While the a* value of breast meat from CS birds was higher than that from NFW, the b* value of that was higher than S and C birds. The c* values of breast meat from S birds were lower compared with that from the CS treatment. The thigh meat from NFW broilers had higher h* value than that from C and G broilers. The thigh meats of C and CS broilers had higher c* value than that of G birds. The full-fed broilers had higher plasma triglyceride concentration than NFW, S and G birds. The full-fed broilers had higher plasma uric acid and uric acid nitrogen concentrations than S birds. These results show that carbohydrate administration in the drinking water cannot be a good alternative for the FW period before slaughter due to the fact that the carbohydrates do not reduce BW losses and do not lead to increases in meat quality.

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Mean platelet volume (MPV) is a parameter that is obtained from an automatic haemogram device during routine blood count and measures platelet reactivity. Increased platelet volume has been considered to be a risk factor for vascular diseases. The aim of this study was to evaluate the relationship between the presence of varicocele and the MPV, platelet count (PLT) and platelet distribution width (PDW) values. We included 264 patients with a diagnosis of varicocele in Group 1, and 220 patients with no varicocele in Group 2. The varicocele diagnosis was performed both with physical examination findings and colour Doppler ultrasonography (CDU). Mean platelet volume values were statistically significantly high (P < 0.001) whereas PLT and PDW values were statistically significantly low (P = 0.011), (P = 0.008) in the varicocele group compared with the control group respectively. However, no significant correlation was found between MPV and PDW in patients diagnosed with varicocele and the varicocele grade on examination and spermatic vein diameter on CDU. Mean platelet volume, which is used widely to measure the size of platelets and indicates platelet reactivity, can provide guidance in the investigation of varicocele pathophysiology and the relevant vascular pathologies.

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This study was conducted to examine the effect of different levels of humic substances (HS) administered in drinking water on caecal microflora and mineral composition and colour characteristics of breast and thigh meats and the growth performance, carcass and gastrointestinal tract (GIT) traits of broiler chicks. A total of 480 3-d-old broiler chickens were randomly allocated to 4 treatments with 4 cages per treatment and 30 bird (15 males and 15 females) chicks per cage. All birds were fed on commercial basal diet. The control birds (HS0) received drinking water with no additions, whereas birds in the other treatment groups received a drinking water with 7.5 (HS7.5), 15.0 (HS15.0) and 22.5 (HS22.5) g/kg HS. Mush feed were provided on an ad libitum basis. Body weight and feed intake of broilers were determined at d 0, 21, and 42, and feed conversion ratio was calculated. On d 42, 4 broilers (2 males and 2 females) from each cage were slaughtered and the breast and thigh meats were collected for mineral composition and quality measurements. Performance, carcass and GIT traits and caecal microbial population of broiler chicks at d 42 were not affected by the dietary treatments. The lightness (L*) of breast and thigh meat decreased in broilers supplemented with 15 and 22.5 g/kg HS in drinking water. Although the redness (a*) of breast meat increased, yellowness of thigh meat decreased in broilers supplemented with 15 and 22.5 g/kg HS in drinking water (P < 0.05). In conclusion, the 15 and 22.5 g/kg HS administration in drinking water can be applied for broiler chicks to maintain growth performance and improve meat quality without changing caecal microflora.

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To evaluate color [lightness (L*), redness (a*), and yellowness (b*)], water-holding capacity (WHC), and pH values, and for proximate analysis of breast and thigh meats from slow-growing (Bronze; B), fast-growing (Hybrid; H), and medium-growing (crosses; H × B) turkey genotypes raised with or without outdoor access, 36 turkeys (2 females and 2 males from each replicate) per housing system or 12, 16, and 8 turkeys per B, H, and H × B genotype, slaughtered at 17 and 21 wk of age, respectively, were used. Therefore, data were analyzed as a factorial arrangement (2 × 3 × 2 × 2) of treatments. All birds were provided with the same starter, grower, and finisher feeds. Muscle samples were collected at 12 h postmortem for evaluation of meat quality and proximate analysis. Outdoor access increased the a* value and protein content of the breast muscle (P < 0.05) and the b* value of the thigh muscle (P < 0.01). The B and H genotypes had higher (P < 0.01) L* values for the breast meat than did the H × B genotype, whereas the B genotype had lower a* (P < 0.01) and pH (P < 0.01) values for the breast meat or a higher (P < 0.05) pH value for the thigh muscle compared with the H genotype. The breast meat of the B genotype was more yellow (P < 0.01) than that of the H and H × B genotype. Thigh meat from the H genotype had a higher L* value and a lower a* value than did thigh meat from the other genotypes (P < 0.01). Thigh meat from the H × B genotype was higher in protein and lower in fat than was thigh meat from the B and H genotypes, respectively (P < 0.05). No interaction effect of housing system and genotype was observed on the parameters studied (P > 0.05). These results show that housing system did not affect the main quality parameters (pH, water-holding capacity, and L* values) of either muscle, and that genotype created more differences in terms of these parameters.

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The present study aimed at evaluating the effects of different doses of humic substances (HS) in waterer pan on broiler performances and meat quality. For the trial, 480 chicks (ROSS 308) were allocated into four groups (HS0, HS150, HS300 and HS450) of 120 equally mixed-sex birds, each receiving a drinking water supplemented with 0, 150, 300 and 450 ppm of a humic acid (provided by 0.0%, 0.5%, 1.0% and 1.5% of HS, v/v) respectively. At the age of 21 and 42 days, feed consumption and live weight were taken. At the age of 42 days, 16 birds per treatment were slaughtered, dressing percentages, digestive tract traits were evaluated and samples of breast (pectoralis major) and thigh (iliotibialis) meat were taken to evaluate the changes in colour characteristics (CIE L*, a* and b*) and chemical composition. The body weight gain increased by the HS300 in relation to the HS0 and HS450, and decreased by the HS450 in relation to the HS0. The feed consumption of birds in the HS450 and feed efficiency of birds in the HS300 were lower and higher than those in the other treatments respectively. The carcass weight of broilers in the HS150 and HS300 were higher than those of in the HS0 and HS450. The carcass weight decreased by the HS450 in relation to the other treatments. The dressing percentage increased by the HS150 in relation to the HS450. The HS450 resulted in breast and thigh meat colour that were lighter than those found in the HS0 birds. The HS450 increased fat content of thigh meat in relation to the HS0. It was concluded that the humic acid supplementation at 300 ppm and 450 ppm appears to have a measurable impact on live performance improving feed efficiency and lightness of breast and thigh meat colours in broilers respectively.

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1. This study was conducted to assess the impact of genotype and outdoor access (and gender when appropriate) on growth rate and carcass yield. 2. One slow-growing genotype (Bronze; B, n = 129), a commercial fast-growing genotype (Hybrid; H, n = 186) and a medium-growing genotype (crosses; H x B, n = 78) were housed (straight-run) for 21 weeks of age. Each genotype was assigned to 3 pens of 20-23 birds each and raised in indoor floor pens in a curtain-sided house with ventilation fans; the B, H and H x B genotypes were also assigned to 3, 4 and 2 floor pens (2 m(2) approximately) with outdoor access (during daylight hours), respectively. All animals were fed ad libitum a commercial diet and birds were commercially processed. After reaching the commercial live weight at 17 and 21 weeks of age, 4 birds (two females and two males) per replicate were slaughtered to determine the yields of carcass, breast, and thigh, abdominal fat and edible inner organs. Gastrointestinal tract characteristics such as length and weight of whole gut were analysed. 3. No significant interaction between genotype and housing systems was observed. Significant differences were found for all traits among genotypes. The live weight was higher in H and H x B genotypes from 7 to 15 weeks and from 7 to 21 weeks of age compared to B genotype, respectively. The live weight of H genotype was higher than that of H x B genotype from 15 to 21 weeks of age. The carcass weight and yield, and the relative weights of the edible inner organs and whole gut, and the relative length of whole gut were significantly affected by genotype and sex of birds. Differences in growth performance, relative weight of breast meat, wings or thighs between birds raised on barn or free-range were negligible. In conclusion, while the development in growth performances was related to strain, the traits at slaughter were related to both strain and sex, and were unaffected by housing system.

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To determine the effects of insemination season (IS; dry: May through October and rainy: November through April), lactation number (LN; heifers, primiparous or multiparous) and their interaction on non-return rate (NRR), conception rate (CR) to first-service and pregnancy loss (PL) of Jersey cattle in Turkey, over 1468 lactation records from 510 Jersey dairy cows were used. There was an interaction between LN x IS on NRR, CR to first-service (p < 0.01) and PL (p < 0.05). The NRR and CR to first-service of heifers inseminated during dry season and of primiparous inseminated during rainy season were higher (p < 0.01) than that of multiparous inseminated during both season. The NRR and CR of heifers and primiparous were higher (p < 0.05) than that of multiparous cows. The insemination of heifers during rainy season and of primiparous and multiparous during dry season increased (p < 0.01) the PL compared to the inseminations of heifers during dry season and of primiparous during rainy season. The PL was lower (p < 0.05) in primiparous than multiparous cows. The results demonstrate that the heifers have high fertility than the lactating, especially multiparous and that IS appeared to have a measurable impact on traits relating to conception of Jersey cattle in the each LN.

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To investigate the changes in characteristics (CIE L*, a* and b*) of liver colour as well as growth, carcass and digestive tract of broilers as influenced by stage and schedule of feed restriction, 400 two-week-old broilers (Ross 308) were randomly assigned to 20 floor pens. Four replicate pens of 20 equally mixed sex birds per pen were randomly allotted each of the five treatments. Birds in control were fed ad libitum. Birds in QFRM and QFRL were restricted 75% of ad libitum intake (quantitative feed restriction, QFR) for 10 days. Birds in FTRM and FTRL were fed ad libitum for 7 days per a period of 10 days with third, fifth and seventh days of feed withdrawal (feeding time restriction, FTR). The restrictions in the QFRM and FTRM started on day 14 (at middle age, M) while the restrictions in the QFRL and FTRL started on day 31 (at late age, L). The restricted broilers consumed less feed than the full-fed birds (p < 0.05). The QFRM and FTRM birds consumed less feed than QFRL and FTRL birds (p < 0.05). The body weight gain decreased by the QFRL and FTRL, the feed efficiency increased by the QFRM and FTRM compared to other treatments (p < 0.05). The QFRL and FTRL increased a* and b* values of liver and the relative weights of gut and liver, and the FTRL increased the L* value of liver compared to other treatments (p < 0.05). The QFRL and FTRL decreased the relative weight of abdominal fat compared to the control (p < 0.05). In conclusion, (i) the restricted feeding at middle stage improved feed efficiency; (ii) the restricted feeding at later stage decreased growth rate and abdominal fat; and (iii) limited-time feeding during later period caused a measurable variation in the liver colour.

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BACKGROUND: Hemodialysis (HD) patients are exposed to oxidative stress which contributes to cardiovascular disease (CVD). The purpose of this study was to investigate the oxidative and antioxidative status in HD patients with (CVD+, n = 38) and without (CVD-, n = 67) prevalent CVD. PATIENTS AND METHODS: A total of 105 HD patients and 21 healthy controls were assessed for lipid peroxidation indices (plasma malondialdehyde (MDA)), oxidizability of apolipoprotein B-containing lipoproteins (apo B-deltaMDA) and red blood cells (RBC-MDA) together with various components of the antioxidant system in plasma (paraoxonase/arylesterase activities, total carotenoids, vitamins C and E) and RBC (superoxide dismutase and glutathione peroxidase activities). RESULTS: Plasma MDA and RBC-MDA were significantly higher, vitamin C and total carotenoid levels were significantly lower in both CVD+ and CVD- HD groups than in the control group. Plasma MDA levels were significantly higher and serum paraoxonase activity, uric acid and albumin levels were significantly lower in patients with CVD+ HD patients compared to those of the CVD- patients. CONCLUSION: This study suggests that the elevated level of plasma MDA and the lower activity of paraoxonase could contribute to the increased incidence of cardiovascular disease in hemodialysis patients.

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Twenty-one growing male Karayaka sheep were divided into three groups of seven at 12 months of age. All groups received a commercial feed (60%) and roughage (40%). One group received grass hay as roughage (control, C), the other two groups received a mixture in which 40 (0.40SBP) or 60% (0.60SBP) sugar beet pulp (SBP) was used in partial replacement of grass hay in the C group during 56 days. Dry matter (DM) intake and DM conversion index (kg DM per kg weight gain) of the C group were higher (P<0.01) than that of the 0.40SBP and 0.60SBP groups. While the 0.60SBP increased (P<0.05) the L* of meat from the Karayaka young rams, the 0.40SBP or 0.60SBP decreased the ultimate pH (P<0.05) and cooking loss (P<0.01) compared to the C group. Means for redness (a*), yellowness (b*), Hue (H*) and Chroma (C*) and sensory characteristics indicated that there were no significant differences between dietary treatments. Inclusion of 60% SBP increased the palmitic (P<0.02) and linoleic (P<0.01) acid contents of the meat, it decreased (P<0.01) stearic, oleic and arachidonic acid contents compared to the C group. The 0.40SBP also increased (P<0.01) the linoleic acid content of the meat, but it decreased (P<0.01) stearic and arachidonic acid contents compared to the control. Although meat from young rams fed 0.60SBP diet had a higher value of lightness and linoleic acid, and a lower value of cooking loss than those from animals fed the C diet, sensory evaluation did not discriminate the meat from young rams fed with control and/or SBP diets. Therefore, grass hay can be replaced by 60% of SBP in the growing diet for Karayaka rams without a reduction in meat quality.