RESUMEN
The safety and immunological activity of P. aeruginosa vaccine were experimentally evaluated. The vaccine was prepared on the basis of the antigens of P. aeruginosa extracellular slime which was accumulated in medium K-4, obtained with the use of original technology. The immunization of animals with P. aeruginosa vaccine induced the synthesis of antibodies. The introduction of the vaccine in 2 or 3 injections resulted in a high level of antibody formation, differing with the use of various strains. Hyperimmune sera, obtained by the multiple immunization of rabbits with P. aeruginosa vaccine, ensured high protection of mice from P. aeruginosa infection. The vaccine proved to be safe when evaluated in experiments of acute and chronic toxicity, made on laboratory animals.
Asunto(s)
Antígenos Bacterianos/inmunología , Vacunas Bacterianas/administración & dosificación , Infecciones por Pseudomonas/prevención & control , Pseudomonas aeruginosa/inmunología , Vacunación , Animales , Anticuerpos Antibacterianos/biosíntesis , Especificidad de Anticuerpos , Antígenos Bacterianos/toxicidad , Vacunas Bacterianas/aislamiento & purificación , Vacunas Bacterianas/toxicidad , Medios de Cultivo , Hidroxilamina , Inyecciones Subcutáneas , Ratones , Infecciones por Pseudomonas/inmunología , Pseudomonas aeruginosa/crecimiento & desarrollo , ConejosRESUMEN
The possibility of using experimental culture medium K-4 prepared on the basis of casein hydrolysate peptides with the isoelectric point 4.1 for obtaining antigens from P. aeruginosa strains was evaluated. Two antigenic fractions were isolated from the culture fluid containing extracellular slime. The study of the toxicity of the antigenic preparations revealed that one of these fractions had low toxicity for mice (the second antigenic fraction was highly toxic). The former P. aeruginosa antigenic fraction was used for obtaining pyocyanic vaccine. One vaccination dose of this vaccine contained 0.2 mg of the antigen adsorbed on aluminum hydroxide. Pyocyanic vaccine ensured the active protection of mice challenged with P. aeruginosa homologous and heterologous strains.
Asunto(s)
Antígenos Bacterianos/administración & dosificación , Medios de Cultivo/química , Infecciones por Pseudomonas/prevención & control , Pseudomonas aeruginosa/inmunología , Vacunación , Animales , Antígenos Bacterianos/aislamiento & purificación , Antígenos Bacterianos/toxicidad , Centrifugación , Precipitación Química , Diálisis , Modelos Animales de Enfermedad , Hidroxilamina , Inyecciones Intraperitoneales , Dosificación Letal Mediana , Ratones , Pseudomonas aeruginosa/crecimiento & desarrollo , Vacunas de Subunidad/administración & dosificación , Vacunas de Subunidad/aislamiento & purificación , Vacunas de Subunidad/toxicidadRESUMEN
The test for the evaluation of the toxicity of different types of pertussis preparations as manifested by their in vitro influence on mouse thymic cells (T test) has been finally worked out. The use of the T test has made it possible to reveal the nonstandard character of the production lots of adsorbed diphtheria-pertussis-tetanus vaccines, both whole-cell vaccine and Japanese acellular vaccine. The degree of the in vitro damaging action of pertussis preparations on mouse thymic cells greatly depends on the residual content of Bordetella pertussis nontoxoidized toxin which, in contrast to B. pertussis lipopolysaccharide and filamentous hemagglutinin, produces pronounced cytotoxic action on mouse thymic cells.
Asunto(s)
Vacuna contra la Tos Ferina/toxicidad , Timo/efectos de los fármacos , Animales , Peso Corporal/efectos de los fármacos , Células Cultivadas/efectos de los fármacos , Vacuna contra Difteria, Tétanos y Tos Ferina/toxicidad , Relación Dosis-Respuesta a Droga , Dosificación Letal Mediana , Ratones , Ratones Endogámicos CBA , Timo/citología , Factores de TiempoRESUMEN
The influence exerted by histamine on the capacity of T-lymphocytes for spontaneous rosette formation in intact animals and in animals immunized with adsorbed DPT vaccine and its components has been studied. Histamine at a concentration of 10(-3) M has been found to produce an inhibitory effect on the capacity of lymphocytes in the blood and spleen of guinea pigs immunized with adsorbed DPT vaccine and its components for spontaneous rosette formation. This effect of histamine has proved to be even more pronounced after the immunization of the animals with adsorbed DPT vaccine and Bordetella pertussis suspension, which is probably due to their sensitizing action.
Asunto(s)
Toxoide Diftérico , Histamina/inmunología , Vacuna contra la Tos Ferina , Formación de Roseta , Linfocitos T/inmunología , Toxoide Tetánico , Animales , Vacuna contra Difteria, Tétanos y Tos Ferina , Combinación de Medicamentos , CobayasRESUMEN
The results obtained in the study of the influence of histamine on the capacity of T-lymphocytes of guinea pigs immunized with DPT-vaccine and its components for spontaneous rosette formation are presented. Histamine at a concentration of 10(-3) M has been found to inhibit the capacity of blood and splenic lymphocytes of guinea pigs immunized with adsorbed DPT vaccine for spontaneous rosette formation. The inhibitory effect is more pronounced after the immunization of the animals with adsorbed DPT vaccine and Bordetella pertussis suspension.