RESUMEN
BACKGROUND: The role of BRCA1 in chronic ischemic episodes seems to be pivotal for adverse remodeling and development of ischemic cardiomyopathy, because of its role in DNA repair and apoptosis. The aim of this study was to investigate the role of BRCA-1 in idiopathic dilated cardiomyopathy (IDCM). MATERIAL AND METHODS: The study group (IDCM) comprised myocardial samples from hearts explanted before transplantation owing to IDCM in 10 males (age 44 ± 5.3 years) without clinical symptoms of ischemic heart disease. The control group consisted myocardial fragments taken from 10 male heart valve and pulmonary artery donors with diagnosed cerebral death (age 40 ± 2.3 years). BRCA1 was detected immunohistochemically with rabbit anti-BRCA1 polyclonal antibody. The intensity of BRCA1 expression was semiquantitatively assessed for cardiocytes, small vessels including capillaries, and interstitial cells. The significances between groups were estimated using the Mann-Whitney U test. RESULTS: All IDCM cases were positive and presented nonuniform BRCA1 expression: hypertrophied cardiocytes showed very intense staining and typical cardiomyopathic cardiocytes were stained weakly forming mosaic. Control cases showed weak-to-moderate uniform staining. Intensity of staining was significantly higher in IDCM cardiocytes, whereas small vessels and interstitial were stained similarly in both groups. CONCLUSIONS: IDCM adverse remodeling results in more intense but nonuniform BRCA1 expression. This phenomenon seems to reflect IDCM biology: lower BRCA1 expression in myocytes with lower anti-apoptotic and DNA damage repair activity, with the opposite in hypertrophied myofibers.
Asunto(s)
Cardiomiopatía Dilatada/patología , Ubiquitina-Proteína Ligasas/metabolismo , Remodelación Ventricular/fisiología , Adulto , Apoptosis/fisiología , Biomarcadores/metabolismo , Capilares/metabolismo , Estudios de Casos y Controles , Femenino , Humanos , Masculino , Isquemia Miocárdica/patología , Miocardio/metabolismo , Miocitos Cardíacos/metabolismoRESUMEN
BACKGROUND: The assessing of heart valve viability is critical for the preparing of viable and implantable biological value. Non-viable valves should be cross-linked with various agents whereas viable tissues are suitable for cryoprotection. Cell viability assessment should be rapid, simple, easy and low cost. MATERIAL AND METHODS: A simple method, simultaneous fluorescent staining of both viable and damaged valve cells was described. The material was consisted of 2 groups: a) with ischemic time below 3 hours (5 swine valves), and b) with ischemic time over 3 hours (5 swine valves). The supravital staining of leaflet fragments was conducted with single solution of fluorescein diacetate (living cells staining) and propodium iodide (damaged cells staining). RESULTS: The simultaneous staining predominantly showed green fluorescence typical for living cells and few red damaged cells in the short ischemic time group, whereas the second group showed the predominance of the red stained damaged cells. CONCLUSIONS: The presented one-step supravital staining method is rapid and simple it allows differentiation of the viability of the valve cells, so it may be of value for the routine evaluation of cadaveric valve homografts.
Asunto(s)
Química Clínica/métodos , Válvulas Cardíacas/patología , Animales , Cadáver , Supervivencia Celular , Células Cultivadas , Fluoresceínas/farmacología , Colorantes Fluorescentes/farmacología , Proteínas Fluorescentes Verdes , Humanos , Proteínas Luminiscentes/metabolismo , Isquemia Miocárdica , Porcinos , Temperatura , Factores de TiempoRESUMEN
Bioprosthetic heart valve, derived from human or animal tissues is a commercial, implantable valve characterised by good haemodynamic parameters, low haemolysis ratio and satisfactory durability. Until now, its wide-spread therapeutic use has been limited by the progressing leaflet calcification. The aim of the paper is to present the benefits, adverse reactions as well as problems associated with the improvement of this bioprosthesis.