RESUMEN
A centrifugal method has been evaluated for measuring the strength of Vero Green Monkey kidney cell adhesion to growth surfaces. The centrifugal force necessary to remove cells gave a quantitative measure of cell adhesion and hence the quality of the growth surface. After being subjected to high gravity forces, both the remaining attached cells and the detached cells were viable, indicating the detachment process did not simply rupture the cell. Electron microscope examination of growth surfaces after cell detachment suggested that remnants related to filopodia remained.
Asunto(s)
Adhesión Celular , Animales , Línea Celular , Centrifugación/métodos , Chlorocebus aethiops , Riñón , Microscopía Electrónica de Rastreo , Factores de TiempoRESUMEN
Photoelectron spectroscopic examination of treated plastic surfaces showed that surface oxidation, primarily as carboxyl groups, was responsible for formation of good growth surfaces. Gas-plasma studies indicated that only very short exposures were required and that the effect was confined to a thin surface layer that produced adhesive surfaces. Highly adhesive surfaces were produced using oxidizing chemicals. Studies with a polymeric ester demonstrated the importance of unesterified carboxyl groups for high adhesiveness.
Asunto(s)
Adhesión Celular , Animales , Ácidos Carboxílicos , Línea Celular , Chlorocebus aethiops , Riñón , Oxígeno , PlásticosRESUMEN
Media containing the fluorogenic compound 8-anilino-1-naphthalene sulfonic acid may be used to discriminate between gram-positive and gram-negative bacteria and to differentiate between various species of bacteria. Fluorescent light emitted from colonies of gram-negative bacteria on 8-anilino-1-naphthalene sulfonic acid-containing agar was visually more intense than that on gram-positive bacteria. The emitted light from the gram-negative bacteria differed in wave-lengths from that of light emitted by colonies of gram-positive bacteria. The fluorescent intensity of colonies on complete 8-anilino-1-napthalene sulfonic acid agar supplemented with 1% of single substrates varied depending on the bacterial species, thus allowing the development of profiles used to identify 12 different species.
Asunto(s)
Naftalenosulfonatos de Anilina/farmacología , Bacterias/clasificación , Fluorescencia , Técnicas Bacteriológicas , Pared Celular , Medios de Cultivo , Escherichia coli/efectos de los fármacos , Especificidad de la Especie , Staphylococcus aureus/efectos de los fármacosRESUMEN
A survey of 26 fluorophores revealed 5 which were non-inhibitory to Staphylococcus aureus and Escherichia coli and produced low background and high colony fluorescence.