RESUMEN
The lytic activity of plague phage II, serovar 3, with respect to 1,800 bacterial strains has been studied: 760 Yersinia pestis strains, 262 Y. pseudotuberculosis strains, 252 Y. enterocolitica strains, 166 Escherichia coli strains, 90 Shigella strains and 270 strains of other species. The phage has been found to lyse 81.8% of Y. pestis strains, 1 Y. pseudotuberculosis strain and 1 Y. enterocolitica strain. The representatives of other 19 bacterial species have proved to be resistant to the phage. Though having a wide range of action within Y. pestis, the phage does not lyse most of the strains of the causative agent of plague, isolated in certain natural foci. This fact offers promise for using the phage for the differentiation of Y. pestis.
Asunto(s)
Bacteriólisis , Bacteriófagos/patogenicidad , Tipificación de Bacteriófagos , Bacteriófagos/clasificación , Lisogenia , Especificidad de la Especie , Yersinia pestisRESUMEN
Phage II, isolated from Y. pestis strain 2247 obtained from a desert focus in Central Asia, has been studied. The phage is classified with moderate phages and essentially differs from moderate phages of serovar 2. The sources of isolation, high specificity and the absence of common serological features with presently known Y. pestis phages of serovars 1 and 2 permit the classification of this phage with new serovar 3 of Y. pestis phages.
Asunto(s)
Bacteriófagos/aislamiento & purificación , Animales , Asia Central , Tipificación de Bacteriófagos , Bacteriófagos/clasificación , Bacteriófagos/efectos de la radiación , Bacteriófagos/ultraestructura , Reservorios de Enfermedades , Lisogenia/efectos de la radiación , Microscopía Electrónica , Serotipificación , Siphonaptera/microbiología , Rayos Ultravioleta , Yersinia pestis/clasificaciónRESUMEN
The bacteriophage designated RD2 has been isolated from the sewage in Rostov-on-Don city and studied. The morphology of bacteriophage particles and the biological properties of the bacteriophage make it related to the plague bacteriophage isolated by D'Errel. The molecular masses of the compared bacteriophages are almost identical being 26.4 +/- 0.4 Md for RD2 and 24.7 +/- 0.2 Md for D'Errel bacteriophage. The DNAs of the bacteriophages share 80% of homology and possess 15 nonhomologous regions scattered along the genomes. The phages are serologically related. The DNAs of both bacteriophages give the similar pattern of hydrolysis by restriction endonuclease EcoRV, but have the different sensitivity to many other restriction endonucleases. The protein specter of bacteriophage RD2 contains 18 polypeptides (11 minor ones), while the one of D'Errel bacteriophage contains 7 polypeptides similar in molecular mass with the polypeptides of RD2. The bacteriophage RD2 cannot be considered one of the plague causative agents of bacteriophages since the region where it has been isolated has a long epidemiological and epizootical record of absence of plague.
Asunto(s)
Bacteriófagos/aislamiento & purificación , Microbiología Ambiental , Yersinia pestis/patogenicidad , Tipificación de Bacteriófagos , Bacteriófagos/clasificación , VirulenciaRESUMEN
Four Y. enterocolitica strains (10166, 10373, 2119, 5513) have been studied for the presence of the enzymatic systems of modification-restriction (M-R). As revealed with the use of cross titration, strains 10166 and 10373 contain M-R systems, supposedly of type II.
Asunto(s)
Enzimas de Restricción del ADN/clasificación , Yersinia enterocolitica/enzimología , Adsorción , Lisogenia , Especificidad de la Especie , Fagos T , Yersinia enterocolitica/genéticaAsunto(s)
Receptores Virales , Adsorción , Bacteriófagos/aislamiento & purificación , Sitios de Unión , Pared Celular/análisis , Pared Celular/ultraestructura , Fenómenos Químicos , Química , Colifagos/fisiología , Escherichia coli/fisiología , Klebsiella pneumoniae/fisiología , Lipopolisacáridos/análisis , Lipopolisacáridos/metabolismo , Lipopolisacáridos/fisiología , Mutación , Pseudomonas aeruginosa/análisis , Receptores Virales/fisiología , Salmonella typhimurium/análisis , Especificidad de la Especie , Fagos T/metabolismoRESUMEN
Results of study of the negative colonies morphology, the structure of corpuscles, antigenic properties, specificity, and the action range permitted to refer the phages obtained from 18 E. coli strain, 1 plaque strain, and 1 pseudotoberculosis bacillus strain to the same group and to identify them with plague phages of serological type 2. Isolation of the same phage type from different bacterial species permits to regard them as "polyhostal" ones. E. coli should be considered as the main carrier of phages belonging to serological type 2. It is suggested that phages existing on microbes belonging to different species should be called "polyhostal".