RESUMEN
BACKGROUND: The lack of nasal tip support resulting in poor protection and projection of the nose is a common cause of nasal obstruction and cosmetic impairment. Although septal cartilage is preferred for restoration of nasal tip support, there is often not enough septal cartilage available. We describe an intercrural columellar strut graft in a circumferential soft tissue pocket: the I-Beam technique. METHODOLOGY: In this cohort study over an 8-year period the clinical and biometrical long-term results after restoration of nasal tip support with an autologous auricular cartilage graft, the I-Beam technique, is evaluated. RESULTS: Out of the 72 consecutive septorhinoplasties with the I-Beam technique, we studied 66 patients in the median long-term follow-up of 89 months. In all patients a significant improvement in nasal tip projection was found. A functional improvement in nasal breathing was achieved in 86%. The overall satisfaction with the I-Beam graft was of 83%. No deterioration in smell perception was found after surgery. CONCLUSIONS: The I-Beam technique using back-to-back sutured autologous auricular cartilage is a safe and effective technique and gives long-term structural support and enhances tip projection.
Asunto(s)
Cartílago Auricular/trasplante , Obstrucción Nasal/cirugía , Deformidades Adquiridas Nasales/cirugía , Nariz , Rinoplastia/métodos , Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Obstrucción Nasal/etiología , Nariz/patología , Nariz/cirugía , Deformidades Adquiridas Nasales/complicaciones , Deformidades Adquiridas Nasales/fisiopatología , Satisfacción del Paciente , Recuperación de la Función , Resultado del TratamientoRESUMEN
It is known that the common physiological denominator of the ageing process is an attenuation of functional performance with respect to the situation of young people and adults. However, since the first cohort-based longitudinal studies, it has not been possible to establish a "linear" relationship between age and glomerular filtration in all cases. This does not mean that there is no physiological ageing process at all; in addition to those already elucidated, its mechanisms include cell senescence, podocyte dysfunction, a vitamin D deficiency, and homozygotic forms of the MYH9 gene. The aim of the present work was to analyse the prevalence of chronic kidney disease (CKD) and, where possible, the correlation between CKD, defined by an eGFR < 60 ml/min/1.73 m(2), plasma 25(OH)D3 levels and the MYH9 gene in a population of elderly and very elderly persons. These parameters have not been evaluated previously in populations of elderly and very elderly patients. It is concluded that a moderate decrease in the eGFR occurs with age. This does not imply the presence of CKD in elderly people, since in most individuals the reduced eGFR is not accompanied by anaemia, and no individuals show hypocalcaemia, hyperphosphataemia or a high Alb/Cr ratio. Here we observed a lower Hb level and an elevated Alb/Cr ratio in subjects heterozygotic for the MYH9 gene. This could be interpreted in the sense that the gene could exert some protective effect on renal function, whereas the heterozygotic form (allele A) of the MYH9 gene could be considered a very early marker, a new risk factor for the appearance of CKD, or a sign of renal frailty in elderly people.
Asunto(s)
Envejecimiento , Calcifediol/sangre , ADN/genética , Tasa de Filtración Glomerular/fisiología , Proteínas Motoras Moleculares/genética , Cadenas Pesadas de Miosina/genética , Polimorfismo Genético , Insuficiencia Renal Crónica/genética , Adolescente , Adulto , Factores de Edad , Anciano , Anciano de 80 o más Años , Biomarcadores/sangre , Femenino , Humanos , Masculino , Persona de Mediana Edad , Proteínas Motoras Moleculares/metabolismo , Cadenas Pesadas de Miosina/metabolismo , Insuficiencia Renal Crónica/sangre , Insuficiencia Renal Crónica/fisiopatología , Factores de Riesgo , Adulto JovenRESUMEN
Honey bee (Apis mellifera) colonies of African and European descent were compared for levels of Varroa destructor infestation in 3 different ecological regions in Mexico. The 300 colonies that were studied were located in subtropical, temperate sub-humid, and temperate dry climates. The morphotype and mitotype of adult bees as well as their rates of infestation by varroa mites were determined. Additionally, the number of combs with brood and covered with bees was recorded for each colony. The highest frequency of colonies that were classified as African-derived was found in the subtropical environment, whereas the lowest occurred in the temperate dry region. Overall, the colonies of African genotype had significantly lower mite infestation rates (3.5±0.34%) than the colonies of European genotype (4.7±0.49%) regardless of the region sampled. Significant effects of genotype and region on Varroa infestation rates were evident, and there were no differences in bee population or capped brood between genotypes. Mite infestation levels were significantly lower in the colonies of the temperate dry region than in the colonies of the other 2 regions. These results are discussed within the context of results from studies that were previously conducted in Brazil. This is the first study that demonstrates the effects of Africanization and ecological environment on V. destructor infestation rates in honey bee colonies in North America.
Asunto(s)
Abejas/parasitología , Varroidae , Animales , Ecología , México , Infestaciones por Ácaros , Clima Tropical , Varroidae/genéticaRESUMEN
The management of intractable posterior epistaxis is challenging for any physician. Nasal packing, often combined with use of an endonasal balloon system, is painful for the patient, and torturous to maintain for two to three days. If conservative treatment fails, the most commonly used treatment options are currently invasive procedures such as endoscopic coagulation of bleeding arteries, external ligation and, rarely, embolisation. This paper describes a simple, non-invasive technique of treating posterior epistaxis with hot water irrigation. Technical information is presented, and the benefits of the method are discussed.
Asunto(s)
Epistaxis/terapia , Técnicas Hemostáticas , Calor/uso terapéutico , Cavidad Nasal , Irrigación Terapéutica/métodos , Algoritmos , Atención Ambulatoria , Oclusión con Balón , Edema , Humanos , Mucosa Nasal/fisiopatología , Irrigación Terapéutica/instrumentación , AguaRESUMEN
Introducción: El quiste tirogloso es una de las masas cervicales más frecuentes. En 1 por ciento puede desarrollar un cáncer, siendo el más frecuente el carcinoma papilar tiroideo. Objetivo: Describir la presentación clínica, el diagnóstico, el manejo y la evolución de los casos de carcinoma papilar tiroideo en quiste tirogloso. Material y método: Se revisaron los archivos del Departamento de Patología de la Universidad de Concepción desde 2000 a 2010 analizando las fichas clínicas y el informe de la biopsia. Resultados: Se encontraron 4 casos de carcinoma papilar en quiste tirogloso, todos de sexo femenino con un promedio de edad de 42 años. Todos fueron sometidos a la operación de Sistrunk. Dos casos fueron sometidos a tiroidectomía total y terapia con radioyodo complementaria. Sólo un paciente presentaba un cáncer sincrónico en la tiroides. El seguimiento promedio fue de 4,7 años y no se presentaron recidivas. Conclusiones: Se encontraron 4 casos, se analiza su presentación, diagnóstico, tratamiento y evolución.
Introduction: Thyroglossal duct cyst is one the most frequent cervical masses. Cancer may develop from 1 percent among them, thyroid papillary carcinoma being the most frequent. Aim: To describe the clinical presentation, diagnosis, management and evolution of thyroid papillary carcinoma cases in thyroglossal duct cyst. Material and method: A review was made of the files from the department of pathology of the Universidad de Concepción, since 2000 to 2010, analyzing clinical records and biopsy reports. Results: Four cases of papillary carcinoma in a thyroglossal duct cyst were found, all female with a mean of age of 42 years. All of them underwent Sistrunk procedure. Two cases underwent total thyroidectomy and complementary radioiodine therapy. Only one patient presented synchronic thyroideal cancer. The mean time of follow up consisted of 4,7 years, with no reported recurrence. Conclusions: We found 4 cases, his presentation, diagnosis, treatment and evolution was analyzed.
Asunto(s)
Humanos , Adulto , Femenino , Persona de Mediana Edad , Carcinoma Papilar/cirugía , Carcinoma Papilar/diagnóstico , Neoplasias de la Tiroides/cirugía , Neoplasias de la Tiroides/diagnóstico , Quiste Tirogloso/cirugía , Quiste Tirogloso/diagnóstico , Carcinoma Papilar/patología , Estudios Retrospectivos , Estudios de Seguimiento , Neoplasias de la Tiroides/patología , Quiste Tirogloso/patología , Resultado del TratamientoRESUMEN
We report a patient in Automatic Peritoneal Dialysis (APD) with tuberculous peritonitis by possible peritoneal infection due to the proximity between fallopian tube and the left ovary, a peritoneal liquid culture was constantly negative. The patient presented a bad clinic evolution. Her only medical history was hypercalcemia six months before developing a peritonitis and occasionally nausea and vomits To confirm the diagnosis it was needed a peritoneal biopsy by means of a laparoscopy with a removal of the peritoneal catheter and left anexectomy. Now, the patient is asintomatic in daily home hemodialysis.
Asunto(s)
Diálisis Peritoneal , Peritonitis Tuberculosa/etiología , Enfermedades de los Anexos/diagnóstico , Enfermedades de los Anexos/microbiología , Enfermedades de los Anexos/cirugía , Antibacterianos/administración & dosificación , Antibacterianos/uso terapéutico , Antituberculosos/administración & dosificación , Antituberculosos/uso terapéutico , Terapia Combinada , Quimioterapia Combinada , Reacciones Falso Negativas , Femenino , Humanos , Hipercalcemia/etiología , Hipoalbuminemia/etiología , Fallo Renal Crónico/complicaciones , Fallo Renal Crónico/terapia , Laparoscopía , Persona de Mediana Edad , Quistes Ováricos/complicaciones , Ovariectomía , Peritonitis Tuberculosa/diagnóstico , Peritonitis Tuberculosa/tratamiento farmacológico , Peritonitis Tuberculosa/cirugía , Neumoperitoneo/etiología , Tuberculoma/diagnóstico , Tuberculoma/cirugía , Tuberculosis Urogenital/complicaciones , Tuberculosis Urogenital/cirugíaRESUMEN
No disponible
Asunto(s)
Humanos , Masculino , Anciano , Peritonitis/etiología , Candidiasis/complicaciones , Infecciones Relacionadas con Catéteres/complicaciones , Candida/patogenicidad , Diálisis Peritoneal/efectos adversosRESUMEN
This is the first study where the systematic application of theories and techniques used in mammalian sperm cryopreservation have been applied to honey bee (Apis mellifera L.) semen as a means to improve postthaw viability of cryopreserved sperm. Six newly designed diluents, three cryoprotectants (dimethyl sulfoxide, DMA, glycerol), and five diluent:semen ratios (1:1, 3:1, 6:1, 9:1, and 12:1) were tested. In addition, the sperm freezing tolerance of three honey bee strains was evaluated. Specific protocols were designed to control semen freezing and thawing rates. Sperm motility was assessed visually, whereas sperm viability was assessed using SYBR-14 and propidium iodide fluorescent stains. Diluent treatments did not affect fresh (nonfrozen) sperm viability yet affected fresh sperm motility (P<0.05). Based on these assessments, two diluents were chosen and used in all successive cryopreservation experiments. Using the selected diluents, semen was collected at various diluent:semen ratios, along with one of the three cryoprotectants. Semen collected at high dilution ratios, using a hypotonic antioxidant diluent containing catalase, in combination with dimethyl sulfoxide, provided higher postthaw sperm viability than that of all other combinations tested (68.3+/-5.4%; P<0.05). Using this combination of dilution ratio, diluent, and cryoprotectant, there were no differences among honey bee strains for postthaw sperm viability (P=0.805). Nevertheless, these new semen dilution and freezing methods improved postthaw viability of sperm to levels that could theoretically sustain worker populations in colonies, thus providing potential for further optimization of cryopreservation techniques for the genetic preservation and improvement of honey bee genotypes.
Asunto(s)
Abejas , Criopreservación/veterinaria , Preservación de Semen/veterinaria , Espermatozoides/fisiología , Animales , Antioxidantes , Abejas/genética , Catalasa , Supervivencia Celular , Criopreservación/métodos , Crioprotectores , Dimetilsulfóxido , Colorantes Fluorescentes , Genotipo , Glicerol , Calor , Masculino , Preservación de Semen/métodos , Soluciones , Especificidad de la Especie , Recuento de Espermatozoides , Motilidad EspermáticaRESUMEN
No disponible
No disponible
Asunto(s)
Humanos , Trastornos del Sueño-Vigilia/epidemiología , Insuficiencia Renal Crónica/complicaciones , Diálisis RenalRESUMEN
No disponible
No disponible
Asunto(s)
Humanos , Peritonitis/etiología , Diálisis Peritoneal/efectos adversos , Antibacterianos/uso terapéutico , Protocolos ClínicosRESUMEN
No disponible
No disponible
Asunto(s)
Humanos , Hipertensión/complicaciones , Diálisis Peritoneal/efectos adversos , Trastornos Cronobiológicos/complicaciones , Modalidades HorariasRESUMEN
The fine-needle aspirates are used as the first step in the diagnostic management of thyroid nodules, they are considered cost-effective compared with the other approaches. During 2005 we studied 47 patients with cytology in the preoperative. We compared the FNAB results with the biopsy of the specimen to establish the accuracy of this method. The diagnosis of papillary carcinoma was correctly made in 18 of 47 histologically proven cases. There were no false positive cases. Of the 20 negative cases, 13 had benign diseases; 1 was a false negative with a follicular carcinoma; 3 cases had papillary microcarcinomas and 3 minimally invasive follicular carcinomas. The sensibility and specificity was 100 percent and 72 percent respectively. The positive predictive value was 100 percent and negative predictive value 65 percent. PAAF of the thyroid gland proved to be an excellent strategy in the preoperative diagnosis for the management of the thyroid nodules.
Asunto(s)
Humanos , Masculino , Femenino , Biopsia con Aguja Fina , Técnicas Citológicas , Nódulo Tiroideo/diagnóstico , Indicadores de Calidad de la Atención de SaludRESUMEN
The frozen section is important in breast diagnosis. We compare the results between frozen section and definitive diagnosis attending to biological conduct, margins and centinel lymphnode in 127 cases performed during a year. 94 cases were for diagnostic purpose, The 34 positive cases were confirmed. In 60 negative cases, one had a microscopic focus of ductal carcinoma in situ. The margins were corrected in 54/55, one showed carcinoma in less than 2mm from the border. 30 cases of centinel lymph node we studied, 4 were positive and 26 negative. There were no false positive cases. We found 2 cases with metastasis of 2 and 5 mm. in the definitive biopsy. The sensitivity, specificity, positive and negative predictive value in diagnosis was 94 percent, 100 percent, 100 percent, 96 percent respectively and in centinel lymph node was 66 percent, 100 percent, 100 percent y 92 percent.
Asunto(s)
Humanos , Femenino , Biopsia del Ganglio Linfático Centinela , Biopsia/métodos , Neoplasias de la Mama/patologíaAsunto(s)
Infecciones , Diálisis Peritoneal , Peritonitis , Prototheca , Adulto , Humanos , MasculinoRESUMEN
No disponible
Asunto(s)
Masculino , Adulto , Humanos , Infecciones , Diálisis Peritoneal , Peritonitis , ProtothecaRESUMEN
The defensive behavior of 52 hybrid honeybee (Apis mellifera L.) colonies from four sets of crosses was studied and compared with that of European and Africanized bee colonies. Colonies containing F(1) hybrid workers were obtained through reciprocal crosses between European and Africanized bees. The total number of stings deposited by workers in a moving leather patch in 1 min was recorded. In each of the four sets of crosses, bees from hybrid colonies of Africanized paternity left more stings in leather patches than bees from hybrid colonies of European paternity. Results strongly suggest paternal effects of African origin increasing the defensive behavior of hybrid colonies. Although some degree of dominance was observed for high-defensive behavior in one of the four sets of crosses involving European paternity, most of the dominance effects reported in the literature appear to be the result of paternal effects. Several hypotheses to explain this phenomenon, as well as the implications of these effects on the fitness and breeding of honeybees are discussed.
Asunto(s)
Agresión , Abejas/genética , Animales , Mordeduras y Picaduras , Mecanismos de Defensa , Femenino , Masculino , Conducta PaternaAsunto(s)
Antígeno Ca-125/sangre , Procedimientos Quirúrgicos Ginecológicos , Neoplasias Glandulares y Epiteliales/cirugía , Neoplasias Ováricas/cirugía , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Persona de Mediana Edad , Recurrencia Local de Neoplasia , Neoplasias Glandulares y Epiteliales/sangre , Neoplasias Glandulares y Epiteliales/patología , Neoplasias Ováricas/sangre , Neoplasias Ováricas/patología , Valor Predictivo de las PruebasRESUMEN
We have constructed a bacterial artificial chromosome (BAC) library for a European honey bee strain using the cloning enzyme HindIII in order to develop resources for structural genomics research. The library contains 36,864 clones (ninety-six 384-well plates). A random sampling of 247 clones indicated an average insert size of 113 kb (range = 27 to 213 kb) and 2 empty vectors. Based on an estimated genome size of 270 Mb, this library provides approximately 15 haploid genome equivalents, allowing >99 probability of recovering any specific sequence of interest. High-density colony filters were gridded robotically using a Genetix Q-BOT in a 4 x 4 double-spotted array on 22.5-cm2 filters. Screening of the library with four mapped honey bee genomic clones and two bee cDNA probes identified an average of 21 positive signals per probe, with a range of 7-38 positive signals per probe. An additional screening was performed with nine aphid gene fragments and one Drosophila gene fragment resulting in seven of the nine aphid probes and the Drosophila probe producing positive signals with a range of 1 to 122 positive signals per probe (average of 45). To evaluate the utility of the library for sequence tagged connector analysis, 1152 BAC clones were end sequenced in both forward and reverse directions, giving a total of 2061 successful reads of high quality. End sequences were queried against SWISS-PROT, insect genomic sequence GSS, insect EST, and insect transposable element databases. Results in spreadsheet format from these searches are publicly available at the Clemson University Genomics Institute (CUGI) website in a searchable format (http://www.genome.clemson.edu/projects/stc/bee/AM__Ba/)
Asunto(s)
Animales , Abejas/genética , Cromosomas Artificiales Bacterianos/genética , Biblioteca Genómica , Lugares Marcados de Secuencia , Clonación Molecular/métodos , Genes de Insecto/genética , Hibridación in Situ , Reacción en Cadena de la Polimerasa , Análisis de Secuencia de ADNRESUMEN
We have constructed a bacterial artificial chromosome (BAC) library for a European honey bee strain using the cloning enzyme HindIII in order to develop resources for structural genomics research. The library contains 36,864 clones (ninety-six 384-well plates). A random sampling of 247 clones indicated an average insert size of 113 kb (range = 27 to 213 kb) and 2% empty vectors. Based on an estimated genome size of 270 Mb, this library provides approximately 15 haploid genome equivalents, allowing >99% probability of recovering any specific sequence of interest. High-density colony filters were gridded robotically using a Genetix Q-BOT in a 4 x 4 double-spotted array on 22.5-cm2 filters. Screening of the library with four mapped honey bee genomic clones and two bee cDNA probes identified an average of 21 positive signals per probe, with a range of 7-38 positive signals per probe. An additional screening was performed with nine aphid gene fragments and one Drosophila gene fragment resulting in seven of the nine aphid probes and the Drosophila probe producing positive signals with a range of 1 to 122 positive signals per probe (average of 45). To evaluate the utility of the library for sequence tagged connector analysis, 1152 BAC clones were end sequenced in both forward and reverse directions, giving a total of 2061 successful reads of high quality. End sequences were queried against SWISS-PROT, insect genomic sequence GSS, insect EST, and insect transposable element databases. Results in spreadsheet format from these searches are publicly available at the Clemson University Genomics Institute (CUGI) website in a searchable format (http://www.genome.clemson.edu/projects/stc/bee/AM__Ba/).