RESUMEN
This article describes a combination of methods- a solid-phase enzyme-linked immunosorbent assay (ELISA) combined with an ultramicroanalytical system (UMAS)- that can be used to measure tetanus antitoxin activity in human sera or plasma. The test, which is rapid and permits analysis of 78 samples of serum per reaction plate with a volume of 10 uL of diluted serum per sample, is proposed as an alternative to the traditional biologic assay in mice based on seroneutralization of a known dose of tetanus toxin. The study reported here compared these two procedures, using them both to evaluate 100 sera from de Clinical Laboratory of the General Calixto García Hospital in Havana, Cuba. The two sets of results showed a high degree of correlation (r = 0.99) when subjected to linear regression analysis (95 percent CI = 0.985-0.993). These and other findings indicate that the cheap and rapid ultramicroELISA method can perform certain tasks for which the slower and costlier traditional assay is not well suited, such as field evaluation of tetanus toxoid vaccines and identification of hyperimmune plasmas appropriate for use in producing specific antitetanus immunoglobulin (AU)
Asunto(s)
Antitoxina Tetánica , Ensayo de Inmunoadsorción Enzimática/métodos , Técnicas para Inmunoenzimas , Bioensayo/normas , CubaRESUMEN
This article describes a combination of methods- a solid-phase enzyme-linked immunosorbent assay (ELISA) combined with an ultramicroanalytical system (UMAS)- that can be used to measure tetanus antitoxin activity in human sera or plasma. The test, which is rapid and permits analysis of 78 samples of serum per reaction plate with a volume of 10 uL of diluted serum per sample, is proposed as an alternative to the traditional biologic assay in mice based on seroneutralization of a known dose of tetanus toxin. The study reported here compared these two procedures, using them both to evaluate 100 sera from de Clinical Laboratory of the General Calixto García Hospital in Havana, Cuba. The two sets of results showed a high degree of correlation (r = 0.99) when subjected to linear regression analysis (95 percent CI = 0.985-0.993). These and other findings indicate that the cheap and rapid ultramicroELISA method can perform certain tasks for which the slower and costlier traditional assay is not well suited, such as field evaluation of tetanus toxoid vaccines and identification of hyperimmune plasmas appropriate for use in producing specific antitetanus immunoglobulin (AU)
This article was also published in Spanish in the Bol. Oficina Sanit. Panam. Vol. 119(2):113-20, 1995
Asunto(s)
Antitoxina Tetánica , Ensayo de Inmunoadsorción Enzimática , Técnicas para Inmunoenzimas , Bioensayo , CubaRESUMEN
Se describe un inmunoensayo enzimático en fase sólida (ELISA) basada en el método indirecto combinado con el sistema ultramicroanalítico (SUMA), para medir la actividad de antitoxina tetánica en suero humano. La prueba, que es rápida y permite analizar 78 muestras de suero por placa de reacción con un volumen de 10 L de suero diluido por muestra, se propone como alternativa al ensayo biológico tradicional en ratones basado en la seroneutralización de una dosis conocida de toxina tetánica. Los resultados de ambas pruebas mostraron, además, una correlación muy satisfactoria cuando se sometieron a análisis de regresión lineal (r=0,99;IC96 por ciento: 0,985 a 0,993). Se recomienda la prueba de ultramicro ELISA para evaluar en el terreno vacunas a base d toxoide tetánico e identificar plasmas hiperinmunes apropiados para producir inmunoglobulina antitétanica