RESUMEN
1297C/T polymorphism of CYP4F2 gene was studied in 108 patients with chronic liver diseases. No significant correlation with predisposition to rapid liver cirrhosis was revealed without consideration for cirrhosis etiology (OR=0.93, 95% CI=0.28-2.99, p=0.885). In patients with viral cirrhosis, a tendency to association of 1297T allele genotypes with rapid cirrhosis development was found (OR=6.0, 95% CI=0.28-382.64, p=0.222). At the same time, CYP4F2 1297T allele was associated with mild (Child-Pugh class A-B) cirrhosis (OR=2.9, p=0.044).
Asunto(s)
Ácidos Araquidónicos/metabolismo , Agonistas de Receptores de Cannabinoides/metabolismo , Sistema Enzimático del Citocromo P-450/genética , Endocannabinoides/metabolismo , Cirrosis Hepática/genética , Alcamidas Poliinsaturadas/metabolismo , Adolescente , Adulto , Anciano , Familia 4 del Citocromo P450 , Femenino , Frecuencia de los Genes , Predisposición Genética a la Enfermedad , Genotipo , Humanos , Masculino , Persona de Mediana Edad , Polimorfismo de Nucleótido Simple , Adulto JovenRESUMEN
Immunohistochemical and PCR analysis of the structure of lipid-containing hepatocytes and replication of chronic hepatitis C viral particles revealed no positive correlation between the presence of HCV RNA in blood specimens, number of infected hepatocytes (by expression of HCV NS3Ag) with clinical biochemical parameters and intensity of structural changes in biopsy specimens. Small-vesicular subcytolemmal lipid infiltration of hepatocytes was detected, presumably associated with hepatitis C virus replication phase. Characteristic subcytolemmal location of the lipid droplets can reflect their involvement in virus transport from the cell and indicate a virus-induced nature of lipid metabolism disorders.
Asunto(s)
Hepatitis C Crónica/patología , Hepatocitos/virología , Metabolismo de los Lípidos , Hígado/patología , Adulto , Anciano , Femenino , Genotipo , Hepacivirus/genética , Hepacivirus/crecimiento & desarrollo , Hepatitis C Crónica/virología , Hepatocitos/patología , Humanos , Hígado/citología , Hígado/virología , Masculino , Persona de Mediana Edad , ARN Viral/sangre , Proteínas no Estructurales Virales/biosíntesis , Replicación Viral , Adulto JovenRESUMEN
Patients with infectious viral or toxic cirrhosis of the liver participated in complex clinical pathomorphological and molecular-genetic study aimed at the search for markers of predisposition to accelerated liver fibrosis, in which the xenobiotic biotransformation system is involved. The results demonstrate association between CYP2D6 (1846G/A) genotype and rapid cirrhosis development and indicate the necessity of studying the mechanisms underlying this association.
Asunto(s)
Citocromo P-450 CYP2D6/genética , Hepatitis B/genética , Hepatitis C/genética , Hepatitis Alcohólica/genética , Cirrosis Hepática/genética , Hígado/patología , Adulto , Anciano , Progresión de la Enfermedad , Femenino , Marcadores Genéticos , Predisposición Genética a la Enfermedad , Hepacivirus/fisiología , Hepatitis B/complicaciones , Hepatitis B/patología , Hepatitis B/virología , Virus de la Hepatitis B/fisiología , Hepatitis C/complicaciones , Hepatitis C/patología , Hepatitis C/virología , Hepatitis Alcohólica/complicaciones , Hepatitis Alcohólica/patología , Heterocigoto , Homocigoto , Humanos , Hígado/enzimología , Hígado/virología , Cirrosis Hepática/etiología , Cirrosis Hepática/patología , Cirrosis Hepática/virología , Masculino , Persona de Mediana Edad , Polimorfismo de Nucleótido Simple , Siberia , Población BlancaRESUMEN
The stereological values of the structural density of hepatocyte cytoplasmic organelles were similar in chronic mixed HCV+HBV infection irrespective of the form of HBV infection (seropositive or latent). High incidence of HBsAg- and HBeAg-negative forms of HBV infection determines the leading role of PCR diagnosis and studies of liver biopsy specimens with detection of HBcAg structural markers and HBV DNA in native liver tissue in the diagnosis of mixed HCV+HBV infection.
Asunto(s)
ADN Viral/sangre , Hepatitis B Crónica/patología , Hepatitis C Crónica/patología , Hepatocitos/ultraestructura , Hígado/patología , Adolescente , Adulto , Anciano , Biopsia , Coinfección , Hepacivirus/fisiología , Antígenos del Núcleo de la Hepatitis B/sangre , Antígenos de Superficie de la Hepatitis B/sangre , Antígenos e de la Hepatitis B/sangre , Virus de la Hepatitis B/fisiología , Hepatitis B Crónica/sangre , Hepatitis B Crónica/diagnóstico , Hepatitis B Crónica/virología , Hepatitis C Crónica/sangre , Hepatitis C Crónica/diagnóstico , Hepatitis C Crónica/virología , Hepatocitos/virología , Humanos , Técnicas para Inmunoenzimas , Hígado/virología , Microscopía Electrónica , Persona de Mediana Edad , Orgánulos/ultraestructura , Orgánulos/virología , Reacción en Cadena de la PolimerasaRESUMEN
We present results of pathomorphological study of the mucosa epithelium in various parts of the gastrointestinal tract in Crohn's disease. Particular attention was paid to structural transformation of epithelial cells. Barrier dysfunction of the gastrointestinal mucosa was shown to be one of the major pathogenetic factors for Crohn's disease.
Asunto(s)
Enfermedad de Crohn/patología , Mucosa Gástrica/patología , Tracto Gastrointestinal/patología , Adolescente , Adulto , Enfermedad de Crohn/fisiopatología , Células Epiteliales/patología , Femenino , Mucosa Gástrica/fisiopatología , Tracto Gastrointestinal/fisiopatología , Humanos , Masculino , Persona de Mediana Edad , Adulto JovenRESUMEN
Structural reorganization of endotheliocytes was studied on models of various pathological processes: placental dysfunction, glomerular pathology, vibration syndrome, antiphospholipid syndrome, and diffuse angiokeratoma, all of these characterized by endothelial insufficiency. Universal modification of endothelial associations was revealed. It included a chain of stereotypical reactions: from degeneration alternating with compensatory hypertrophy to subsequent atrophy and death of endotheliocytes. The time course of the process was confirmed by the results of light microscopy in combination with ultrastructural examination and by evaluation of the biosynthetic reactions by in vitro radioautography.