RESUMEN
This study investigated the antiradical and antioxidant potential of the three families of lipopeptides (i.e., surfactin, mycosubtilin, and plipastatin/fengycin) produced by Bacillus subtilis strains. The antiradical/antioxidant activities of highly purified lipopeptides were studied in acellular models using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anion ( O 2 . - ), hydrogen peroxide, (H2O2) and hydroxyl radical (HO.). At a lipopeptide concentration of 500 mg.L-1, the maximum inhibition of DPPH reached 22.88% (obtained for plipastatin). Moreover, the scavenging effects of O 2 . - , H2O2, and HO. at the highest concentration tested (250 mg.L-1) were found to be 6, 21, and 3% for surfactin, 19, 9, and 15% for mycosubtilin, 21, 18, and 59% for plipastatin, 21, 31, and 61% for the mixture of surfactin/plipastatin, and 13, 16, and 15% for the mixture of surfactin/mycosubtilin, respectively. These results showed that plipastatin was the best candidate due to its antioxidant activities.
RESUMEN
In this study, a specific fraction of food waste, i.e. depackaging waste, was studied as substrate for hydrogen production by dark fermentation. During storage and transport of this liquid mixture, inhibitory compounds like acids or alcohol might be produced by endogenous flora. A factorial fractional design based on the composition of the substrate was used to determine the best condition to convert this substrate into hydrogen. First results indicated that the consortium used might convert high quantity of lactate into hydrogen. A batch culture confirmed that lactate was used as the main carbon source and a global yield of 0.4molH2·mollactate-1 was obtained. This study demonstrated the ability of the consortium tested to convert different carbon sources (carbohydrates or lactate) with good efficiency. These data represented an important parameter in the prospect of using an industrial substrate whose composition is liable to vary according to the conditions of storage and transport.