RESUMEN
Primary graft dysfunction (PGD) is a possible risk factor for bronchiolitis obliterans syndrome (BOS) following lung transplantation; however, the mechanism for any such association is poorly understood. Based on the association of TGF-ß with acute and chronic inflammatory disorders, we hypothesized that it might play a role in the continuum between PGD and BOS. Thus, the association between PGD and BOS was assessed in a single-center cohort of lung transplant recipients. Bronchoalveolar lavage fluid concentrations of TGF-ß and procollagen collected within 24 h of transplantation were compared across the spectrum of PGD, and incorporated into Cox models of BOS. Immunohistochemistry localized expression of TGF-ß and its receptor in early lung biopsies posttransplant. We found an association between PGD and BOS in both bilateral and single lung recipients with a hazard ratio of 3.07 (95% CI 1.76-5.38) for the most severe form of PGD. TGF-ß and procollagen concentrations were elevated during PGD (p < 0.01), and associated with increased rates of BOS. Expression of TGF-ß and its receptor localized to allograft infiltrating mononuclear and stromal cells, and the airway epithelium. These findings validate the association between PGD and the subsequent development of BOS, and suggest that this association may be mediated by receptor/TGF-ß biology.
Asunto(s)
Biomarcadores/metabolismo , Bronquiolitis Obliterante/diagnóstico , Rechazo de Injerto/diagnóstico , Trasplante de Pulmón/efectos adversos , Disfunción Primaria del Injerto/diagnóstico , Factor de Crecimiento Transformador beta/metabolismo , Anciano , Bronquiolitis Obliterante/etiología , Bronquiolitis Obliterante/metabolismo , Estudios de Casos y Controles , Femenino , Estudios de Seguimiento , Rechazo de Injerto/sangre , Rechazo de Injerto/etiología , Supervivencia de Injerto , Humanos , Técnicas para Inmunoenzimas , Enfermedades Pulmonares/cirugía , Masculino , Persona de Mediana Edad , Complicaciones Posoperatorias , Disfunción Primaria del Injerto/etiología , Disfunción Primaria del Injerto/metabolismo , Pronóstico , Estudios Prospectivos , Estudios Retrospectivos , Medición de Riesgo , Factores de Riesgo , Índice de Severidad de la EnfermedadRESUMEN
Dendritic cells (DCs) play a key role in initiating alloimmunity yet the substances that activate them during the host response to transplantation remain elusive. In this study we examined the potential roles of endogenous innate immune agonists in activating dendritic cell-dependent alloimmunity. Using a murine in vitro culture system, we show that 135 KDa fragments of the extracellular matrix glycosaminoglycan hyaluronan induce dendritic cell maturation and initiate alloimmunity. Priming of alloimmunity by hyaluronan-activated DCs was dependent on signaling via TIR-associated protein, a Toll-like receptor (TLR) adaptor downstream of TLRs 2 and 4. However, this effect was independent of alternate TLR adaptors, MyD88 or Trif. Using an in vivo murine transplant model, we show that hyaluronan accumulated during skin transplant rejection. Examination of human lung transplant recipients demonstrated that increased levels of intragraft hyaluronan were associated with bronchiolitis obliterans syndrome. In conclusion, our study suggests that fragments of hyaluronan can act as innate immune agonists that activate alloimmunity.
Asunto(s)
Células Dendríticas/inmunología , Ácido Hialurónico/inmunología , Isoantígenos/inmunología , Oligosacáridos/inmunología , Trasplante de Piel/inmunología , Animales , Células Cultivadas , Ácido Hialurónico/metabolismo , Ácido Hialurónico/farmacología , Inflamación/fisiopatología , Trasplante de Pulmón/inmunología , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Factor 88 de Diferenciación Mieloide/deficiencia , Factor 88 de Diferenciación Mieloide/genética , Factor 88 de Diferenciación Mieloide/fisiología , Oligosacáridos/farmacología , Receptor Toll-Like 4/inmunología , Receptor Toll-Like 8/inmunología , Factor de Necrosis Tumoral alfa/biosíntesisRESUMEN
Noninflammatory structural alterations, variously referred to as airway remodeling, are well documented in the asthmatic airway. However, the pathogenesis of these alterations, the importance of airway remodeling in generating the asthma phenotype, and the natural history of airway remodeling responses have not been adequately defined. Because exaggerated cytokine production is a characteristic feature of the asthmatic airway, we used constitutive and inducible overexpression transgenic systems to investigate the contributions that interleukin 11 (IL-11) and IL-13 might make to airway remodeling responses. These studies demonstrated that both cytokines produce responses in the murine airway with features similar to those in human asthmatic tissues. IL-11 caused airway fibrosis with the enhanced accumulation of interstitial collagens, myocytes, and myofibroblasts. IL-13 caused mucous metaplasia, enhanced mucin gene expression, enhanced tissue hyaluronic acid accumulation, and subepithelial fibrosis. Importantly, IL-11 was detected most readily in tissues from asthmatic subjects with severe airway remodeling that was similar to that seen in the IL-11 transgenic mice. In addition, IL-11 was shown to inhibit asthma-like inflammation while stimulating airway fibrosis. This suggests that IL-11 elaboration is, in part, an attempt at airway healing. Last, a novel triple transgenic system is described that allows transgene expression to be regulated in a true "on/off" manner. This system may be useful in defining the reversibility of transgene-induced airway remodeling responses.
Asunto(s)
Asma/genética , Asma/patología , Interleucina-11/fisiología , Interleucina-13/fisiología , Pulmón/patología , Animales , Antibacterianos/farmacología , Asma/fisiopatología , Enfermedad Crónica , Modelos Animales de Enfermedad , Expresión Génica , Humanos , Inflamación/genética , Inflamación/patología , Interleucina-11/genética , Interleucina-13/genética , Pulmón/metabolismo , Ratones , Ratones Transgénicos , Fenotipo , ARN Mensajero/análisis , Tetraciclina/farmacología , Transcripción GenéticaRESUMEN
Pulmonary inflammation and fibrosis are characterized by increased turnover and production of the extracellular matrix as well as an impairment of lung fibrinolytic activity. Although fragments of the extracellular matrix component hyaluronan induce macrophage production of inflammatory mediators, the effect of hyaluronan on the fibrinolytic mediators plasminogen activator inhibitor (PAI)-1 and urokinase-type plasminogen activator (uPA) is unknown. This study demonstrates that hyaluronan fragments augment steady-state mRNA, protein, and inhibitory activity of PAI-1 as well as diminish the baseline levels of uPA mRNA and inhibit uPA activity in an alveolar macrophage cell line. Hyaluronan fragments alter macrophage expression of PAI-1 and uPA at the level of gene transcription. Similarly, hyaluronan fragments augment PAI-1 and diminish uPA mRNA levels in freshly isolated inflammatory alveolar macrophages from bleomycin-treated rats. These data suggest that hyaluronan fragments influence alveolar macrophage expression of PAI-1 and uPA and may be a mechanism for regulating fibrinolytic activity during lung inflammation.
Asunto(s)
Regulación de la Expresión Génica/fisiología , Ácido Hialurónico/farmacología , Macrófagos Alveolares/fisiología , Macrófagos/fisiología , Inhibidor 1 de Activador Plasminogénico/genética , Activador de Plasminógeno de Tipo Uroquinasa/genética , Amilorida/farmacología , Animales , Células de la Médula Ósea/citología , Núcleo Celular/efectos de los fármacos , Núcleo Celular/metabolismo , Células Cultivadas , Regulación de la Expresión Génica/efectos de los fármacos , Lipopolisacáridos/farmacología , Macrófagos/efectos de los fármacos , Macrófagos Alveolares/efectos de los fármacos , Ratones , Biosíntesis de Proteínas/efectos de los fármacos , ARN Mensajero/metabolismo , Ratas , Transcripción Genética/efectos de los fármacosAsunto(s)
Citocinas/metabolismo , Endotelina-1/metabolismo , Pulmón/metabolismo , Pulmón/patología , Fibrosis Pulmonar/metabolismo , Animales , Citocinas/genética , Endotelina-1/genética , Humanos , Inflamación/genética , Inflamación/inmunología , Inflamación/metabolismo , Inflamación/patología , Pulmón/inmunología , Ratones , Ratones Transgénicos , Fibrosis Pulmonar/genética , Fibrosis Pulmonar/inmunología , Fibrosis Pulmonar/patología , Receptores de Endotelina/genética , Receptores de Endotelina/metabolismo , Subgrupos de Linfocitos T/inmunologíaRESUMEN
There is increasing evidence implicating activated macrophages in the pathogenesis of interstitial and other lung diseases. We investigated whether there was a unique pattern of cell surface expression that constituted a disease-specific phenotype on alveolar macrophages from patients with interstitial lung disease (ILD). Macrophage cell surface receptor expression of 19 selected markers was assessed by indirect immunofluorescence and flow cytometry in bronchoalveolar lavage (BAL) fluids from patients with idiopathic pulmonary fibrosis (IPF, n = 4), scleroderma (SCL-ILD, n = 14), mild asthma (n = 7), allergy without asthma (n = 2), and normal subjects (n = 9). There was increased expression of adhesion receptors (CD11c, CD29, CD36, CD44, CD49e, CD54), receptors involved in signal transduction and/or inflammation (CD13, CD45, CD53), and other markers (CD9, CD52, CD71, CD98, HLA Class I) on macrophages from ILD patients compared to the non-ILD group. Most markers upregulated on macrophages in ILD were significantly inversely correlated with clinical parameters of disease activity such as FEV(1), FVC, and DL(CO) and positively correlated with numbers of BAL neutrophils and eosinophils. Increased expression of several cell surface markers suggests that activated alveolar macrophages may contribute to the pathophysiology of IPF and SCL-ILD.
Asunto(s)
Enfermedades Pulmonares Intersticiales/patología , Macrófagos Alveolares/metabolismo , Fenotipo , Adulto , Anciano , Líquido del Lavado Bronquioalveolar/citología , Eosinófilos/citología , Femenino , Expresión Génica , Humanos , Recuento de Leucocitos , Enfermedades Pulmonares Intersticiales/genética , Masculino , Persona de Mediana Edad , Neutrófilos/citología , Receptores de Superficie Celular/genéticaAsunto(s)
Ácido Hialurónico/farmacología , Macrófagos Alveolares/metabolismo , Inhibidor 1 de Activador Plasminogénico/metabolismo , Síndrome de Dificultad Respiratoria/metabolismo , Activador de Plasminógeno de Tipo Uroquinasa/metabolismo , Animales , Células Cultivadas , Fibrinólisis , Ratones , Síndrome de Dificultad Respiratoria/sangreRESUMEN
Although the metalloproteinase murine metalloelastase (MME) has been implicated in lung disorders such as emphysema and pulmonary fibrosis, the mechanisms regulating MME expression are unclear. Low m.w. fragments of the extracellular matrix component hyaluronan (HA) that accumulate at sites of lung inflammation are capable of inducing inflammatory gene expression in macrophages (Mphi). The purpose of this study was to examine the effect of HA fragments on the expression of MME in alveolar Mphi. The mouse alveolar Mphi cell line MH-S was stimulated with HA fragments over time, total RNA was isolated, and Northern blot analysis was performed. HA fragments induced MME mRNA in a time-dependent fashion, with maximal levels at 6 h. HA fragments also induced MME protein expression as well as enzyme activity. The induction of MME gene expression was specific for low m.w. HA fragments and dependent upon new protein synthesis; it occurred at the level of gene transcription. We also examined the effect of HA fragments on MME expression in inflammatory alveolar Mphi from bleomycin-injured rat lungs. Although normal rat alveolar Mphi did not express MME mRNA in response to HA fragments, alveolar Mphi from the bleomycin-treated rats responded to HA fragment stimulation by increasing MME mRNA levels. Furthermore, baseline and HA fragment-induced MME gene expression in alveolar Mphi from bleomycin-treated rats was inhibited by IFN-gamma. These data suggest that HA fragments may be an important mechanism for the expression of MME by Mphi in inflammatory lung disorders.
Asunto(s)
Ácido Hialurónico/farmacología , Macrófagos Alveolares/enzimología , Metaloendopeptidasas/biosíntesis , Animales , Bleomicina/farmacología , Línea Celular , Modelos Animales de Enfermedad , Relación Dosis-Respuesta Inmunológica , Activación Enzimática/efectos de los fármacos , Activación Enzimática/inmunología , Inducción Enzimática/efectos de los fármacos , Inducción Enzimática/inmunología , Femenino , Humanos , Macrófagos Alveolares/efectos de los fármacos , Masculino , Metaloproteinasa 12 de la Matriz , Metaloendopeptidasas/genética , Metaloendopeptidasas/metabolismo , Ratones , Ratones Endogámicos C3H , Peso Molecular , Biosíntesis de Proteínas , ARN Mensajero/biosíntesis , Ratas , Ratas Sprague-DawleyRESUMEN
Hallmarks of chronic inflammation and tissue fibrosis are increased influx of activated inflammatory cells, mediator release, and increased turnover and production of the extracellular matrix (ECM). Recent evidence has suggested that fragments of the ECM component hyaluronan play a role in chronic inflammation by inducing macrophage expression of chemokines. Interferon-gamma (IFN-gamma), an important regulator of macrophage functions, has been shown to induce the C-X-C chemokines Mig and IP-10. These chemokines affect T-cell recruitment and inhibit angiogenesis. The purpose of this investigation was to determine the effect of hyaluronan (HA) on IFN-gamma-induced Mig and IP-10 expression in mouse macrophages. We found a marked synergy between HA and IFN-gamma on Mig and IP-10 mRNA and protein expression in mouse macrophages. This was most significant with Mig, which was not induced by HA alone. The synergy was specific for HA, was not dependent on new protein synthesis, was not mediated by tumor necrosis factor-alpha, was selective for Mig and IP-10, and occurred at the level of gene transcription. These data suggest that the ECM component HA may influence chronic inflammatory states by working in concert with IFN-gamma to alter macrophage chemokine expression.
Asunto(s)
Quimiocinas CXC/biosíntesis , Ácido Hialurónico/farmacología , Péptidos y Proteínas de Señalización Intercelular , Interferón gamma/farmacología , Macrófagos Alveolares/efectos de los fármacos , Animales , Quimiocina CXCL10 , Quimiocina CXCL9 , Quimiocinas CXC/genética , Cicloheximida/farmacología , Relación Dosis-Respuesta a Droga , Sinergismo Farmacológico , Matriz Extracelular , Regulación de la Expresión Génica , Ratones , ARN Mensajero/análisis , Transcripción GenéticaRESUMEN
Inflammation is associated with production of cytokines and chemokines that recruit and activate inflammatory cells. Interleukin (IL) 12 produced by macrophages in response to various stimuli is a potent inducer of interferon (IFN) gamma production. IFN-gamma, in turn, markedly enhances IL-12 production. Although the immune response is typically self-limiting, the mechanisms involved are unclear. We demonstrate that IFN-gamma inhibits production of chemokines (macrophage inflammatory proteins MIP-1alpha and MIP-1beta). Furthermore, pre-exposure to tumor necrosis factor (TNF) inhibited IFN-gamma priming for production of high levels of IL-12 by macrophages in vitro. Inhibition of IL-12 by TNF can be mediated by both IL-10-dependent and IL-10-independent mechanisms. To determine whether TNF inhibition of IFN-gamma-induced IL-12 production contributed to the resolution of an inflammatory response in vivo, the response of TNF+/+ and TNF-/- mice injected with Corynebacterium parvum were compared. TNF-/- mice developed a delayed, but vigorous, inflammatory response leading to death, whereas TNF+/+ mice exhibited a prompt response that resolved. Serum IL-12 levels were elevated 3-fold in C. parvum-treated TNF-/- mice compared with TNF+/+ mice. Treatment with a neutralizing anti-IL-12 antibody led to resolution of the response to C. parvum in TNF-/- mice. We conclude that the role of TNF in limiting the extent and duration of inflammatory responses in vivo involves its capacity to regulate macrophage IL-12 production. IFN-gamma inhibition of chemokine production and inhibition of IFN-gamma-induced IL-12 production by TNF provide potential mechanisms by which these cytokines can exert anti-inflammatory/repair function(s).
Asunto(s)
Antiinflamatorios/farmacología , Interferón gamma/farmacología , Interleucina-12/biosíntesis , Macrófagos Peritoneales/inmunología , Factor de Necrosis Tumoral alfa/farmacología , Animales , Células Cultivadas , Quimiocina CCL3 , Quimiocina CCL4 , Antagonismo de Drogas , Femenino , Inflamación/prevención & control , Interleucina-12/antagonistas & inhibidores , Proteínas Inflamatorias de Macrófagos/biosíntesis , Ratones , Ratones Endogámicos C3H , Ratones Endogámicos C57BLRESUMEN
Turnover of the extracellular matrix (ECM), activation of macrophages, and accumulation of chemokines/cytokines are all hallmarks of chronic inflammation. Extracellular matrix components, such as hyaluronan (HA), have recently been shown to influence macrophage effector functions, such as the release of inflammatory chemokines and cytokines. Although low m.w. fragments of the glycosaminoglycan HA induce macrophages to secrete numerous inflammatory mediators, the mechanisms regulating ECM-induced macrophage activation are poorly understood. We have examined the effects of IL-10 and IFN-gamma on HA-induced chemokine gene expression in primary mouse macrophages. We found that IL-10 and IFN-gamma independently inhibit HA-induced expression of macrophage inflammatory protein-1alpha (MIP-1alpha), MIP-1beta, and KC at both the mRNA and protein levels. Whereas IL-10 inhibited most of the HA-induced chemokines tested, IFN-gamma selectively inhibited only MIP-1alpha, MIP-1beta, and KC. This inhibition did not require prestimulation and occurred even when the cytokines were added up to 3 h after stimulation with HA. For MIP-1alpha, the inhibition by IFN-gamma occurred at the level of transcription, whereas IL-10 predominantly decreased the stability of MIP-1alpha mRNA. IFN-gamma and IL-10 equally inhibited macrophage expression of MIP-1beta mRNA at the level of transcription, but MIP-1beta mRNA stability was decreased to a greater extent by IL-10. These data identify a previously unrecognized role for IL-10 and IFN-gamma as regulators of ECM-induced macrophage expression of inflammatory chemokines.
Asunto(s)
Regulación de la Expresión Génica/efectos de los fármacos , Ácido Hialurónico/farmacología , Interferón gamma/farmacología , Interleucina-10/farmacología , Proteínas Inflamatorias de Macrófagos/genética , Macrófagos/metabolismo , Animales , Quimiocina CCL3 , Quimiocina CCL4 , Cicloheximida/farmacología , Dactinomicina/farmacología , Femenino , Ratones , Ratones Endogámicos C3H , ARN Mensajero/análisis , Factores de TiempoRESUMEN
Obesity is associated with an increased incidence of infection, diabetes, and cardiovascular disease, which together account for most obesity-related morbidity and mortality. Decreased expression of leptin or of functional leptin receptors results in hyperphagia, decreased energy expenditure, and obesity. It is unclear, however, whether defective leptin-dependent signal transduction directly promotes any of the conditions that frequently complicate obesity. Abnormalities in tumor necrosis factor alpha expression have been noted in each of the above comorbid conditions, so leptin deficiency could promote these complications if leptin had immunoregulatory activity. Studies of rodents with genetic abnormalities in leptin or leptin receptors revealed obesity-related deficits in macrophage phagocytosis and the expression of proinflammatory cytokines both in vivo and in vitro. Exogenous leptin up-regulated both phagocytosis and the production of proinflammatory cytokines. These results identify an important and novel function for leptin: up-regulation of inflammatory immune responses, which may provide a common pathogenetic mechanism that contributes to several of the major complications of obesity.
Asunto(s)
Citocinas/biosíntesis , Macrófagos/inmunología , Fagocitosis/fisiología , Proteínas/fisiología , Receptores de Superficie Celular , Animales , Proteínas Portadoras/genética , Proteínas Portadoras/metabolismo , Citocinas/genética , Regulación de la Expresión Génica , Inflamación/metabolismo , Inflamación/fisiopatología , Leptina , Lipopolisacáridos/farmacología , Macrófagos/efectos de los fármacos , Ratones , Ratones Obesos , Receptores de Leptina , Proteínas RecombinantesRESUMEN
Hepatic injury and chronic wounding are characterized by increased synthesis of extracellular matrix proteins including hyaluronan (HA). Recently, it has been recognized that low-molecular-weight fragments of HA, but not native HA (e.g., high-molecular-weight HA), induce inflammatory gene expression, and activate the transcriptional regulator, nuclear factor kappaB (NF-kappaB). The inducible isoform of nitric oxide synthase (iNOS) is induced by cytokines and/or lipopolysaccharide (LPS) through the NF-kappaB signal transduction pathway. Because of this association, we hypothesized that HA fragments might also stimulate iNOS gene transcription. The aims of this study were therefore to determine whether HA or HA fragments induced iNOS in hepatic cells, and to characterize the signaling pathway. HA fragments (100 microg/mL) markedly stimulated iNOS messenger RNA (mRNA) in endothelial and Kupffer cells, but minimally induced this mRNA in hepatocytes and stellate cells. High-molecular-weight HA (200 microg/mL) had no effect on iNOS mRNA in any cell type. The addition of interferon gamma (IFN-gamma) to HA fragments resulted in stimulation of iNOS mRNA 2-, 3-, 4-, and 10-fold above that for HA fragments alone in hepatocytes, endothelial, Kupffer, and stellate cells, respectively. The combination of HA fragments and LPS did not result in an incremental increase in iNOS mRNA induction. iNOS protein and nitrite levels (used as a measure of NO production and NOS enzymatic activity) paralleled closely iNOS mRNA expression and increased proportionally to HA fragment concentration in a dose-dependent fashion. At 1 hour following stimulation, NF-kappaB DNA binding activity was detected in extracts from Kupffer cells stimulated with HA fragments, but not in those exposed to media alone or to high-molecular-weight HA. Finally, inhibitors of NF-kappaB blocked HA fragment-dependent iNOS mRNA induction in Kupffer and sinusoidal endothelial cells. The data indicate that HA fragments, but not high-molecular-weight HA, induce iNOS in liver, having the greatest effects on endothelial and Kupffer cells. We speculate that HA fragments may be an important stimulus for NO production in various forms of liver disease, particularly as a cofactor with inflammatory cytokines.
Asunto(s)
Ácido Hialurónico/farmacología , Hígado/efectos de los fármacos , Hígado/enzimología , Óxido Nítrico Sintasa/metabolismo , Fragmentos de Péptidos/farmacología , Animales , Antioxidantes/farmacología , Células Cultivadas , ADN/metabolismo , Inducción Enzimática , Hígado/citología , Masculino , FN-kappa B/antagonistas & inhibidores , FN-kappa B/metabolismo , Óxido Nítrico Sintasa de Tipo II , Prolina/análogos & derivados , Prolina/farmacología , Ratas , Ratas Sprague-Dawley , Inhibidores de Serina Proteinasa/farmacología , Transducción de Señal/fisiología , Tiocarbamatos/farmacología , Clorometilcetona de Tosilfenilalanila/farmacologíaRESUMEN
Sjögren's syndrome is one of the most common systemic rheumatic diseases. Pulmonary disease is prevalent in Sjögren's syndrome; respiratory manifestations include chronic cough, obstructive airways disease, pulmonary lymphoma, and interstitial lung disease that may progress to severe pulmonary fibrosis.
Asunto(s)
Enfermedades Autoinmunes , Enfermedades Pulmonares/inmunología , Síndrome de Sjögren/complicaciones , Adulto , Femenino , Humanos , Enfermedades Pulmonares/etiología , Masculino , Persona de Mediana Edad , Síndrome de Sjögren/inmunologíaRESUMEN
Components of the extracellular matrix (ECM) can regulate leukocyte activation and function at inflammatory sites. Low molecular weight fragments of the ECM glycosaminoglycan hyaluronan (LMW-HA) that accumulate in inflammation, but not the ubiquitous high molecular weight form of HA (HMW-HA), have been shown to induce cytokine and/or chemokine production by alveolar and bone-marrow derived macrophages. To determine the cellular requirements for responsiveness to HA, we compared the effects of HMW-HA and LMW-HA on resident and thioglycollate-elicited murine peritoneal macrophages. We demonstrate that treatment of elicited macrophages with LMW-HA, but not with HMW-HA, stimulated production of the chemokines RANTES and macrophage inflammatory protein-1alpha and -1beta. Further, we demonstrate that LMW-HA induced the production of biologically active IL-12, a proinflammatory cytokine not previously known to be regulated by cell-matrix interactions. The LMW-HA-induced production of IL-12 by elicited macrophages was inhibited by an anti-CD44 mAb that blocks HA binding. In contrast to elicited macrophages, freshly explanted resident peritoneal macrophages did not respond to LMW-HA. However, preculture in vitro before stimulation led to adhesion-dependent priming for LMW-HA-induced cytokine and chemokine production by resident macrophages. These results provide further evidence of the potential importance of CD44/LMW-HA interactions in regulating the immune response at sites of inflammation and demonstrate that the state of differentiation of macrophages may determine their sensitivities to matrix components.
Asunto(s)
Quimiocinas/biosíntesis , Regulación de la Expresión Génica/efectos de los fármacos , Receptores de Hialuranos/fisiología , Ácido Hialurónico/farmacología , Interleucina-12/biosíntesis , Macrófagos Peritoneales/efectos de los fármacos , Animales , Anticuerpos Monoclonales/farmacología , Adhesión Celular , Quimiocinas/genética , Matriz Extracelular/fisiología , Femenino , Receptores de Hialuranos/inmunología , Ácido Hialurónico/química , Inflamación/fisiopatología , Interferón gamma/farmacología , Interleucina-12/genética , Activación de Macrófagos , Macrófagos Peritoneales/metabolismo , Ratones , Ratones Endogámicos C3H , Peso Molecular , Peritoneo/citología , Peritonitis/inducido químicamente , Peritonitis/patología , Tioglicolatos/toxicidadRESUMEN
Activated macrophages play a critical role in controlling chronic tissue inflammation through the release of a variety of mediators including cytokines, chemokines, growth factors, active lipids, reactive oxygen, and nitrogen species. The mechanisms that regulate macrophage activation in chronic inflammation are poorly understood. A hallmark of chronic inflammation is the turnover of extracellular matrix components, and recent work has suggested that interactions with the extracellular matrix can exert important influences on macrophage effector functions. We have examined the effect of low molecular weight fragments of the extracellular matrix glycosaminoglycan hyaluronan (HA) on the induction of nitric-oxide synthase (iNOS) in macrophages. We found that HA fragments induce iNOS mRNA, protein and activity alone, and markedly synergize with interferon-gamma to induce iNOS gene expression in murine macrophages. In addition, we found that resident tissue alveolar macrophages respond minimally, but inflammatory alveolar macrophages exhibit a marked induction in iNOS expression in response to HA fragments. Finally, we demonstrate that the mechanism of HA fragment-induced expression of iNOS requires activation of the transcriptional regulator nuclear factor kappaB. These data support the hypothesis that HA may be an important regulator of macrophage activation at sites of chronic tissue inflammation.
Asunto(s)
Ácido Hialurónico/farmacología , Macrófagos Alveolares/efectos de los fármacos , FN-kappa B/metabolismo , Óxido Nítrico Sintasa/biosíntesis , Animales , Médula Ósea/efectos de los fármacos , Médula Ósea/enzimología , Células de la Médula Ósea , Línea Celular , Inducción Enzimática , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Ácido Hialurónico/química , Mediadores de Inflamación , Macrófagos Alveolares/enzimología , Ratones , Mutagénesis Sitio-Dirigida , Óxido Nítrico Sintasa/genéticaRESUMEN
Hyaluronan (HA) is a glycosaminoglycan constituent of extracellular matrix. In its native form HA exists as a high molecular weight polymer, but during inflammation lower molecular weight fragments accumulate. We have identified a collection of inflammatory genes induced in macrophages by HA fragments but not by high molecular weight HA. These include several members of the chemokine gene family: macrophage inflammatory protein-1alpha, macrophage inflammatory protein-1beta, cytokine responsive gene-2, monocyte chemoattractant protein-1, and regulated on activation, normal T cell expressed and secreted. HA fragments as small as hexamers are capable of inducing expression of these genes in a mouse alveolar macrophage cell line, and monoclonal antibody to the HA receptor CD44 completely blocks binding of fluorescein-labeled HA to these cells and significantly inhibits HA-induced gene expression. We also investigated the ability of HA fragments to induce chemokine gene expression in human alveolar macrophages from patients with idiopathic pulmonary fibrosis and found that interleukin-8 mRNA is markedly induced. These data support the hypothesis that HA fragments generated during inflammation induce the expression of macrophage genes which are important in the development and maintenance of the inflammatory response.
Asunto(s)
Quimiocina CCL2/genética , Regulación de la Expresión Génica/inmunología , Ácido Hialurónico/inmunología , Proteínas Inflamatorias de Macrófagos/genética , Macrófagos Alveolares/inmunología , Monocinas/genética , Animales , Anticuerpos Bloqueadores/inmunología , Anticuerpos Monoclonales/inmunología , Northern Blotting , Lavado Broncoalveolar , Células Cultivadas , Quimiocina CCL4 , Quimiocina CCL5/genética , Quimiocina CXCL10 , Gliceraldehído-3-Fosfato Deshidrogenasas/genética , Humanos , Receptores de Hialuranos/inmunología , Inflamación/genética , Interleucina-8/genética , Ratones , Fibrosis Pulmonar/genética , Fibrosis Pulmonar/inmunología , ARN Mensajero/análisis , ARN Mensajero/biosíntesisRESUMEN
Sarcoidosis is a multisystem granulomatous disease of unknown etiology characterized by the expansion of activated oligoclonal CD4+ T cells and macrophages at sites of disease. To investigate the immunopathogenesis of sarcoidosis, we analyzed patterns of cytokine expression in bronchoalveolar lavage cells and fluid from patients with pulmonary sarcoidosis and idiopathic pulmonary fibrosis and from normal volunteers. We found dominant type 1 cytokine expression, with elevated mRNA and protein levels of IFN-gamma, but not IL-4, in sarcoid lung cells and fluid compared with those in normal samples. To define immunoregulatory mechanisms important to this type 1 response, we analyzed the expression of IL-12 and IL-10 in lung cells and fluid. Using semiquantitative PCR, we found significantly higher mRNA expression of the regulated IL-12 p40 subunit, but not IL-10, in sarcoid compared with normal lung cells. Consistent with these observations, strikingly elevated levels of p40 protein were found in sarcoid compared with normal bronchoalveolar lavage fluid. Unstimulated and Staphylococcus aureus-stimulated sarcoid alveolar macrophages produced greater amounts of IL-12 than normal alveolar macrophages when cultured in vitro. We hypothesize that sarcoidosis is a Th1-mediated disease driven by chronic, dysregulated production of IL-12 at sites of disease.
Asunto(s)
Citocinas/metabolismo , Interleucina-12/metabolismo , Sarcoidosis Pulmonar/inmunología , Células TH1/inmunología , Adulto , Anciano , Líquido del Lavado Bronquioalveolar/química , Femenino , Humanos , Interleucina-10/metabolismo , Pulmón/inmunología , Macrófagos Alveolares/inmunología , Masculino , Persona de Mediana Edad , Células Th2/inmunologíaRESUMEN
Macrophages play an important role in the acute tissue inflammatory response through the release of cytokines and growth factors in response to stimuli such as lipopolysaccharide (LPS). Macrophage inflammatory effector functions are also influenced by interactions with the extracellular matrix (ECM). Such macrophage-ECM interactions may be important in regulating chronic inflammatory responses. Recent evidence has suggested that hyaluronan (HA), a glycosaminoglycan (GAG) component of ECM can induce inflammatory gene expression in murine macrophages. HA exists in its native form as a large polymer, but is found as smaller fragments under inflammatory conditions. The NF-kappa B/I-kappa B transcriptional regulatory system has been shown to be a critical component of the host inflammatory response. We examined the effects of high molecular weight HA and lower molecular weight HA fragments on NF-kappa B activation in mouse macrophages. Only the smaller HA fragments were found to activate NF-kappa B DNA binding activity. After HA stimulation, I-kappa B alpha mRNA was induced and I-kappa B alpha protein levels, which initially decreased, were restored. The induction of I-kappa Balpha expression was not observed for other GAGs. The time course of I-kappa B alpha protein regeneration in response to HA fragments was consistent with an autoregulatory mechanism. In support of this mechanism, in vitro translated murine I-kappa B alpha inhibited HA fragment-induced NF-kappa B DNA binding activity. The NF-kappa B DNA binding complex in HA-stimulated extracts was found to contain p50 and p65 subunits. Activation of the NF-kappa B/I-kappa B system in macrophages by ECM fragments may be an important mechanism for propagating the tissue inflammatory response.
Asunto(s)
Proteínas de Unión al ADN/metabolismo , Ácido Hialurónico/farmacología , Proteínas I-kappa B , Macrófagos/fisiología , FN-kappa B/metabolismo , Oligopéptidos/farmacología , Animales , Secuencia de Bases , Secuencia de Consenso , Cicloheximida/farmacología , ADN Complementario , Proteínas de Unión al ADN/biosíntesis , Homeostasis , Humanos , Ácido Hialurónico/química , Cadenas kappa de Inmunoglobulina/genética , Cinética , Lipopolisacáridos/farmacología , Macrófagos/efectos de los fármacos , Ratones , Datos de Secuencia Molecular , Inhibidor NF-kappaB alfa , FN-kappa B/antagonistas & inhibidores , Regiones Promotoras Genéticas , Biosíntesis de Proteínas , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/metabolismo , Transcripción GenéticaRESUMEN
Increased synthesis of insulin-like growth factor I (IGF-I), a fibroblast growth factor, is induced in murine macrophages by TNF-alpha. TNF-alpha also induces macrophages to express cytocidal activity, but only during costimulation with IFNs. Since prostaglandin E2 (PGE2) is known to inhibit macrophage cytocidal activity, its possible reciprocal enhancement of IGF-I synthesis was examined. PGE2 or dibutyryl cyclic AMP (dbcAMP) stimulated the synthesis of IGF-I similarly to TNF-alpha in magnitude and time course. TNF-alpha did not increase IGF-I synthesis by first inducing PGE2 synthesis, because indomethacin was unable to block the effect of TNF-alpha. PGE2 did not stimulate IGF-I synthesis by first inducing TNF-alpha production, because 1) anti-TNF-alpha Ab did not block PGE2-induced IGF-I synthesis, and 2) PGE2 down-regulated TNF-alpha mRNA levels and did not affect levels of the cytokine in supernatants. Moreover, the difference in the induction of IGF-I was observed at the level of signal transduction, in that PGE2 and dbcAMP increased cAMP-dependent protein kinase (PKA) activity, whereas TNF-alpha stimulated the mitogen-activated protein (MAP) kinase pathway. Divergence between the two pathways was also noted in the regulation of IGF-I at the mRNA level, and an additive effect on IGF-I synthesis was observed when cells were incubated with the combination of TNF-alpha plus PGE2 or dbcAMP. Collectively, these data suggest that TNF-alpha and PGE2 stimulate IGF-I synthesis in macrophages by two separate pathways, and that PGE2 acts as a positive stimulus for IGF-I synthesis through a cyclic AMP/PKA pathway.