RESUMEN

BACKGROUND: In studies of development and aging, the expression of many genes has been shown to undergo drastic changes at mRNA and protein levels. The connection between mRNA and protein expression level changes, as well as the role of posttranscriptional regulation in controlling expression level changes in postnatal development and aging, remains largely unexplored. RESULTS: Here, we survey mRNA and protein expression changes in the prefrontal cortex of humans and rhesus macaques over developmental and aging intervals of both species' lifespans. We find substantial decoupling of mRNA and protein expression levels in aging, but not in development. Genes showing increased mRNA/protein disparity in primate brain aging form expression patterns conserved between humans and macaques and are enriched in specific functions involving mammalian target of rapamycin (mTOR) signaling, mitochondrial function and neurodegeneration. Mechanistically, aging-dependent mRNA/protein expression decoupling could be linked to a specific set of RNA binding proteins and, to a lesser extent, to specific microRNAs. CONCLUSIONS: Increased decoupling of mRNA and protein expression profiles observed in human and macaque brain aging results in specific co-expression profiles composed of genes with shared functions and shared regulatory signals linked to specific posttranscriptional regulators. Genes targeted and predicted to be targeted by the aging-dependent posttranscriptional regulation are associated with biological processes known to play important roles in aging and lifespan extension. These results indicate the potential importance of posttranscriptional regulation in modulating aging-dependent changes in humans and other species.

Asunto(s)

Envejecimiento/genética , MicroARNs/biosíntesis , Corteza Prefrontal/metabolismo , Proteínas de Unión al ARN/biosíntesis , Envejecimiento/patología , Animales , Perfilación de la Expresión Génica , Regulación del Desarrollo de la Expresión Génica , Humanos , Macaca mulatta , MicroARNs/genética , Corteza Prefrontal/crecimiento & desarrollo , ARN Mensajero/biosíntesis , Proteínas de Unión al ARN/genética , Transducción de Señal

RESUMEN

Each phase of eukaryotic cell cycle is tightly controlled by multicomponent regulatory networks based on complex relationships of protein phosphorylation. In order to better understand the relationships between kinases and their substrate proteins during the progression of cell cycle, we analyzed phosphoproteome of HeLa cells during G1, S, and G2/M phases of cell cycle using our developed quantitative phosphoproteomic approaches. A total of 4776 high-confidence phosphorylation sites (phosphosites) in 1177 proteins were identified. Bioinformatics analysis for predicting kinase groups revealed that 46 kinase groups could be assigned to 4321 phosphosites. The majority of phosphoproteins harboring two or more phosphosites could be phosphorylated by different kinase groups, in which nine major kinase groups accounted for more than 90% phosphosites. Further analyses showed that approximately half of the examined two phosphosite combinations were correlatively regulated, regardless of whether the kinase groups were same or not. In general, the majority of proteins containing correlated phosphosites had solely co-regulated or counter-regulated phosphosites, and co-regulation was significantly more frequent than counter-regulation, suggesting that the former may be more important for regulating the cell cycle. In conclusion, our findings provide new insights into the complex regulatory mechanisms of protein phosphorylation networks during eukaryotic cell cycle.

Asunto(s)

Ciclo Celular/fisiología , Fosfoproteínas/metabolismo , Proteínas Quinasas/metabolismo , Bases de Datos de Proteínas , Células HeLa/citología , Células HeLa/metabolismo , Humanos , Redes y Vías Metabólicas , Fosforilación , Proteómica/métodos

RESUMEN

We described a workflow involving a combination of titanium dioxide (TiO(2)) enrichment, strong anion exchange (SAX), and strong cation exchange (SCX) fractionation for global phosphoproteome analysis. The workflow proposed TiO(2) -based high efficient enrichment with optimum peptide-to-beads ratio prior to robust IEC fractionation. With the optimum peptide-to-beads ratio, offline TiO(2) enrichment provides high selectivity and large sample loading capacity compared with online TiO(2) chromatography. The eluate with highly enriched phosphopeptides is then subjected to online SAX and SCX fractionation coupled to RP-LC-MS/MS analysis. The identification of phosphopeptides from SAX, SCX, and flow-through fractions showed high complementary features. Importantly, large amount of multiphosphopeptides could be recovered in SAX fractionations. In total, up to 5063 unique phosphosites were identified from 4557 unique phosphopeptides using 4-mg HeLa cell lysate as the starting material.

Asunto(s)

Cromatografía por Intercambio Iónico/métodos , Cromatografía de Fase Inversa/métodos , Fosfopéptidos/análisis , Proteómica/métodos , Células HeLa , Humanos , Espectrometría de Masas en Tándem/métodos

RESUMEN

Human evolution is characterized by the rapid expansion of brain size and drastic increase in cognitive capabilities. It has long been suggested that these changes were accompanied by modifications of brain metabolism. Indeed, human-specific changes on gene expression or amino acid sequence were reported for a number of metabolic genes, but actual metabolite measurements in humans and apes have remained scarce. Here, we investigate concentrations of more than 100 metabolites in the prefrontal and cerebellar cortex in 49 humans, 11 chimpanzees, and 45 rhesus macaques of different ages using gas chromatography-mass spectrometry (GC-MS). We show that the brain metabolome undergoes substantial changes, both ontogenetically and evolutionarily: 88% of detected metabolites show significant concentration changes with age, whereas 77% of these metabolic changes differ significantly among species. Although overall metabolic divergence reflects phylogenetic relationships among species, we found a fourfold acceleration of metabolic changes in prefrontal cortex compared with cerebellum in the human lineage. These human-specific metabolic changes are paralleled by changes in expression patterns of the corresponding enzymes, and affect pathways involved in synaptic transmission, memory, and learning.

Asunto(s)

Evolución Biológica , Metaboloma , Corteza Prefrontal/metabolismo , Proteínas/metabolismo , Humanos

RESUMEN

For large-scale analysis of phosphorylation at proteome-wide scale, a variety of affinity-based strategies have been developed to enrich phosphopeptide. Because each method differed in their specificity of isolation, the global and unbiased enrichment of phosphopeptides remains a major technical challenge in phosphoproteomics. In the present work, we demostrate that the phosphopeptide enrichment method based on an online continuous pH gradient in a strong anion exchange column (SAX method) is highly complementary to the method based on titanium dioxide (TiO2) affinity enrichment. Moreover, we found that the flow-through fraction of either SAX or SCX is very phosphopeptide-rich, which necessitates further analysis by complementary method. Here, we developed a comprehensive phosphopeptides profiling strategy based on anion exchange followed by flow-through enrichment by TiO2 (AFET). In this strategy, SAX method was used as the first separation/enrichment step, which was online coupled with LC-MS/MS. The phosphopeptides in the SAX flow-through fraction were further enriched with TiO2. As a result, a more comprehensive, less biased phosphoproteome was aquired. Careful comparison of four different combination strategies reveal that the AFET method showed the advantages of more identified phosphopeptides, less mass spectrometry analysis time, as well as simple and automatic process step. It is well-suited for robust and reproducible phosphoproteomics, especially in the case of small amounts of sample.

Asunto(s)

Cromatografía por Intercambio Iónico/métodos , Fosfopéptidos/química , Proteómica/métodos , Titanio/química , Resinas de Intercambio Aniónico , Extractos Celulares/química , Células HeLa , Humanos , Concentración de Iones de Hidrógeno , Fragmentos de Péptidos/química , Fragmentos de Péptidos/metabolismo , Fosfopéptidos/aislamiento & purificación , Fosfopéptidos/metabolismo , Fosforilación , Espectrometría de Masas en Tándem , Tripsina/metabolismo

RESUMEN

Lysine acetylation regulates many eukaryotic cellular processes, but its function in prokaryotes is largely unknown. We demonstrated that central metabolism enzymes in Salmonella were acetylated extensively and differentially in response to different carbon sources, concomitantly with changes in cell growth and metabolic flux. The relative activities of key enzymes controlling the direction of glycolysis versus gluconeogenesis and the branching between citrate cycle and glyoxylate bypass were all regulated by acetylation. This modulation is mainly controlled by a pair of lysine acetyltransferase and deacetylase, whose expressions are coordinated with growth status. Reversible acetylation of metabolic enzymes ensure that cells respond environmental changes via promptly sensing cellular energy status and flexibly altering reaction rates or directions. It represents a metabolic regulatory mechanism conserved from bacteria to mammals.

Asunto(s)

Proteínas Bacterianas/metabolismo , Ácido Cítrico/metabolismo , Enzimas/metabolismo , Gluconeogénesis , Glucosa/metabolismo , Glucólisis , Salmonella typhimurium/metabolismo , Acetilación , Acetiltransferasas/química , Acetiltransferasas/genética , Acetiltransferasas/metabolismo , Secuencia de Aminoácidos , Metabolismo Energético , Regulación Bacteriana de la Expresión Génica , Gliceraldehído-3-Fosfato Deshidrogenasas/metabolismo , Histona Desacetilasas del Grupo III/genética , Histona Desacetilasas del Grupo III/metabolismo , Isocitratoliasa/metabolismo , Lisina/metabolismo , Redes y Vías Metabólicas , Complejos Multienzimáticos/metabolismo , Mutación , Procesamiento Proteico-Postraduccional , Proteínas Serina-Treonina Quinasas/metabolismo , Proteínas Recombinantes/metabolismo , Salmonella typhimurium/enzimología , Salmonella typhimurium/genética , Salmonella typhimurium/crecimiento & desarrollo

RESUMEN

Leptospirosis is a widespread zoonotic disease caused by pathogenic spirochetes of the genus Leptospira that infects humans and a wide range of animals. By combining computational prediction and high-accuracy tandem mass spectra, we revised the genome annotation of Leptospira interrogans serovar Lai, a free-living pathogenic spirochete responsible for leptospirosis, providing substantial peptide evidence for novel genes and new gene boundaries. Subsequently, we presented a high-coverage proteome analysis of protein expression and multiple posttranslational modifications (PTMs). Approximately 64.3% of the predicted L. interrogans proteins were cataloged by detecting 2 540 proteins. Meanwhile, a profile of multiple PTMs was concurrently established, containing in total 32 phosphorylated, 46 acetylated and 155 methylated proteins. The PTM systems in the serovar Lai show unique features. Unique eukaryotic-like features of L. interrogans protein modifications were demonstrated in both phosphorylation and arginine methylation. This systematic analysis provides not only comprehensive information of high-coverage protein expression and multiple modifications in prokaryotes but also a view suggesting that the evolutionarily primitive L. interrogans shares significant similarities in protein modification systems with eukaryotes.

Asunto(s)

Leptospira interrogans/metabolismo , Procesamiento Proteico-Postraduccional , Proteoma/análisis , Acetilación , Acetiltransferasas/metabolismo , Algoritmos , Secuencia de Aminoácidos , Animales , Proteínas Bacterianas/metabolismo , Secuencia de Bases , Genoma Bacteriano , Leptospira interrogans/genética , Datos de Secuencia Molecular , Fosforilación , Proteínas Quinasas/metabolismo , Procesamiento Proteico-Postraduccional/fisiología , Proteómica/métodos , Homología de Secuencia de Aminoácido , Spirochaetales/genética , Spirochaetales/metabolismo , Espectrometría de Masas en Tándem

RESUMEN

Titanium dioxide (TiO(2)) has been proven to be a highly efficient strategy and widely used for phosphopeptide enrichment. Many advances have been made recently, including online/offline mode and optimization of sample loading/elution buffer; however, beads usage has rarely been explored. In the current study, we found that the peptide-to-TiO(2) beads ratio was a significant factor for phosphopeptide enrichment, and insufficient or excessive beads could decrease the selectivity. Specifically, for HeLa total cell lysate, the optimum peptide-to-beads ratio is about 1:2-1:8 (mass/mass) to obtain the highest enrichment selectivity and the maximum phosphopeptides identification with single incubation. Pre-experiments are recommended to decide an optimum peptide-to-TiO(2) beads ratio when it comes to different samples. Interestingly, deficient beads can help identify much more multiphosphorylated peptides than the optimum peptide-to-beads ratio by consecutive incubations. Therefore, if multiphosphorylated peptides identification is desired, deficient beads amount is preferred. In addition, consecutive incubation using deficient beads could be used as a fractionation of phosphopeptides besides as an enrichment method.

Asunto(s)

Cromatografía Líquida de Alta Presión , Péptidos/química , Fosfopéptidos/aislamiento & purificación , Titanio/química , Cromatografía Líquida de Alta Presión/instrumentación , Cromatografía Líquida de Alta Presión/métodos , Células HeLa , Humanos , Fosfopéptidos/química , Fosforilación , Unión Proteica , Espectrometría de Masas en Tándem/métodos

RESUMEN

The complexity and diversity of biological samples in proteomics require intensive fractionation ahead of mass spectrometry identification. This work developed a chromatographic method called virtual three-dimensional chromatography to fractionate complex protein mixtures. By alternate elution with different pHs and salt concentrations, we implemented pH and salt steps by turns on a single strong cation exchange column to fully exploit its chromatographic ability. Given standard proteins that were not resolved solely by pH or salt gradient elution could be successfully separated using this combined mode. With a reversed phase column tandem connected behind, we further fractionated as well as desalted proteins as the third dimension. This present strategy could readily be adapted with respect to special complexity of biological samples. Crude plasma without depleting high abundance proteins were fractionated by this three-dimensional mode and then analyzed by reversed phase liquid chromatography coupled with LTQ mass spectrometry. In total, 1933 protein groups with wide dynamic ranges were identified from a single experiment. Some characteristics that correlated to the behavior of proteins on strong cation exchange columns are also discussed.

Asunto(s)

Cromatografía Liquida , Concentración de Iones de Hidrógeno , Proteínas , Sales (Química)/química , Animales , Tampones (Química) , Cromatografía Liquida/instrumentación , Cromatografía Liquida/métodos , Punto Isoeléctrico , Espectrometría de Masas/instrumentación , Espectrometría de Masas/métodos , Plasma/química , Proteínas/análisis , Proteínas/aislamiento & purificación , Reproducibilidad de los Resultados