RESUMEN
The authors demonstrate the possibility of measuring metadon and its metabolites in the urine by high-pressure thin-layer chromatography and reverse-phase thin-layer chromatography on Merck and Russian Sorbton RP2 plates.
Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Cromatografía en Capa Delgada/métodos , Metadona/orina , Cromatografía Líquida de Alta Presión/instrumentación , Cromatografía Líquida de Alta Presión/estadística & datos numéricos , Cromatografía en Capa Delgada/instrumentación , Cromatografía en Capa Delgada/estadística & datos numéricos , Codeína/orina , Dependencia de Heroína/orina , Humanos , Morfina/orinaRESUMEN
A combination of instrumental chromatographic methods high pressure liquid chromatography in the isocratic mode and reverse phase thin-layer chromatography with densitometric ending on Russian Sorbton-RP-2 plates--is conducive to a fuller and more reliable identification of narcotic analgesics in biological fluids (blood and urine).
Asunto(s)
Analgésicos Opioides/orina , Cromatografía Líquida de Alta Presión/métodos , Cromatografía en Capa Delgada/métodos , Cromatografía Líquida de Alta Presión/instrumentación , Cromatografía en Capa Delgada/instrumentación , Codeína/orina , Humanos , Morfina/orina , Papaverina/orinaRESUMEN
A series of methods was developed for improving the reliability of chromatographic analyses and to augment their information content and reproducibility. In high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC) the possibility of utilizing azeotropic and isobaric solvent systems was substantiated. To control variations of the composition of the eluent systems during storage and analysis in TLC and HPLC, gas chromatography (GC) was found to be applicable. To improve the reliability of GC analyses, a membraneless assembly for introduction of samples, which allows false peaks in a chromatogram to be removed, is recommended. In order to augment the reliability and information content of chromatographic analyses of several bioactive compounds, complex chromatographic methods based on GC, TLC and HPLC were found to be desirable.
Asunto(s)
Cromatografía/normas , Cromatografía/instrumentación , Cromatografía de Gases/instrumentación , Cromatografía de Gases/normas , Cromatografía Líquida de Alta Presión/normas , Cromatografía en Capa Delgada/instrumentación , Cromatografía en Capa Delgada/normas , Espectrofotometría UltravioletaRESUMEN
Isolation and partial purification of renin from human kidney was carried out as follows: defatting of the homogenized kidney; extraction with methyl cellosolve; chromatography on DEAE cellulose; affinity chromatography using immobilized pepstain. Activity of the preparation obtained was 0.1 GU/mg of protein. The preparation of human renin might be used for measurement of renin activity in blood of patients.
Asunto(s)
Riñón/análisis , Renina/aislamiento & purificación , Angiotensinógeno/farmacología , Cromatografía de Afinidad/métodos , Cromatografía DEAE-Celulosa/métodos , Interacciones Farmacológicas , Activación Enzimática , Humanos , Renina/farmacologíaRESUMEN
Angiotensinogen (a substrate of renin) was isolated from pig and sheep blood plasma. Content of angiotensinogen was estimated in the preparations obtained by an indirect biological method, which was based on estimation of an increase in blood pressure in rats, caused by angiotensin, liberated from angiotensinogen during incubation with renin. The preparation, obtained by ammonium sulphate fractionation of pig blood plasma, contained 0.4% of angiotensinogen. Angiotensinogen was also isolated from sheep blood plasma using ammonium sulphate fractionation, DEAE-Sephadex chromatography and gel filtration through Sephadex G-100; this preparation contained 10-13% of angiotensinogen.