RESUMEN
OBJECTIVES: To clarify the protective effects of Cichorium glandulosum (CG) extracts on thioacetamide (TAA)-induced rat hepatic fibrosis. MATERIALS AND METHODS: The dry roots of CG were smashed and percolated with 95% ethanol, and the residual was prepared into petroleum ether extract (CG-V), ethyl acetate extract (CG-VI) and n-butyl alcohol extract (CG-VII). Thirty-six Wistar rats were randomly divided into a normal group, a model group, a CG-V group (15 mg/kg), a CG-VI group (3 mg/kg), a CG-VII group (6 mg/kg) and a positive drug group (silibinin capsule, 8 mg/kg). Organ indices and serum levels of glutamic-oxaloacetic and glutamic-pyruvic transaminases of intragastrically administered rats were obtained. Expressions of FN, Smad3, IGFBPrP1 and TGF-ß1 genes were detected by Western Blot and immunohistochemical assays. Apoptosis was examined by TUNEL assay. RESULTS: Hepatic fibrosis of treatment groups was evidently mitigated. Expressions of FN, Smad3 and TGF-ß1 in administration groups were higher than those in normal group, and moreover were significantly higher in CG-V and CG-VII groups than those of model group. Apoptotic index of model group was significantly higher than that of normal group, but indices of CG-V and CG-VII groups were significantly lower than that of model group. Significantly more FN, Smad3 and IGFBPrP1 were expressed in treatment groups than those in normal group. CONCLUSION: CG extracts may function by altering TGF-ß/Smads signal transduction pathway.
RESUMEN
BACKGROUND: Cichorium glandulosum (CG), which is a Compositae family plant, is a commonly used traditional Uighur medicine capable of cleansing liver and being cholagogue, strengthening stomach, promoting digestion, inducing diuresis and reducing edema. OBJECTIVES: To study the liver and spleen indices, the levels of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT), and the histopathological changes. MATERIALS AND METHODS: Rats were intragastrically administered with the extracts of a traditional Uighur medicine Cichorium glandulosum (CG). The expressions of FN, Smard3 IGFBPrPl and TGF-ß1 were detected. RESULTS: The liver and spleen indices of the CG-V group were significantly lower than those of the model group (P < 0.01). The hepatic fibrosis symptoms of the CG-V and CG-VII groups were significantly relieved, and more FN, Smard3 and IGFBPrPl were expressed than those in the normal group. The expressions of FN, Smard3 and TGF-ß1 in all treatment groups were significantly higher than those in the normal group, and the expressions in the CG-V and CG-VII groups were significantly different from those in the model group (P < 0.05). Compared with the normal group, the apoptotic index of the model group was significantly higher, but the indices of the CG-V and CG-VII groups were significantly lower than that of the model group (P < 0.01). CONCLUSIONS: The extracts of CG probably exerted protective effects by influencing the TGF-ß/Smads signal transduction pathway.
Asunto(s)
Proteínas de Unión a Factor de Crecimiento Similar a la Insulina/metabolismo , Cirrosis Hepática Experimental/metabolismo , Hígado/metabolismo , Extractos Vegetales/farmacología , Proteína smad3/metabolismo , Factor de Crecimiento Transformador beta1/metabolismo , Animales , Cichorium intybus , Fibronectinas/metabolismo , Hígado/efectos de los fármacos , Masculino , Ratas , Ratas WistarRESUMEN
Here we present a simple and controlled method for direct fabrication of ordered 2D arrays of magnetic rings. This method utilizes polystyrene-coated magnetite nanoparticles as a solution, and the magnetic rings are fabricated on patterned self-assembled monolayers by dewetting of the solution. Polystyrene-coated magnetite nanoparticles were synthesized by atom-transfer radical polymerization, which promoted the dispersibility and stability of magnetite nanoparticles in chloroform. Magnetic rings were studied using optical photograph, SEM, and magnetic force microscopy. This approach offers a new way for patterning nanoparticulate rings with deliberate control over feature composition, size, as well as interfeature distance.