RESUMEN
OBJECTIVE: To preliminarily understand the genotype characteristics of Toxoplasma gondii in blood of HIV-positive persons in Lincang City, Yunnan Province. METHOD: Two segments of SAG2 gene of T. gondii from blood samples of HIV-positive persons in Lincang City were extracted and amplified by using the nested PCR method and the genotype was identified and compared with the standard strain (Type I) of Toxoplasma gondii. RESULTS: Thirty-five SAG2 genes (241 bp) and 35 SAG2 genes (221 bp) of T. gondii were amplified from 170 blood samples of the HIV-positive people, and 4 of each case were selected and digested with enzyme, then 2 aim gene fragments of each case were chosen and compared with the standard strain (Type I) of T. gondii. The digestion of SAG2 gene (241 bp) showed the genotype of the blood samples was Type I or Type II, and the digestion of SAG2 gene (221 bp) confirmed that the genotype was Type I. CONCLUSION: It is preliminarily confirmed that the genotype of T. gondii in blood of HIV-positive persons in Lincang City, Yunnan Province is Type I.
Asunto(s)
Antígenos de Protozoos/genética , Infecciones por VIH/complicaciones , Proteínas Protozoarias/genética , Toxoplasma/genética , Toxoplasma/aislamiento & purificación , Toxoplasmosis/parasitología , Antígenos de Protozoos/sangre , Secuencia de Bases , China/epidemiología , Genotipo , Humanos , Datos de Secuencia Molecular , Proteínas Protozoarias/sangre , Toxoplasma/metabolismo , Toxoplasmosis/sangre , Toxoplasmosis/epidemiología , Toxoplasmosis/etiologíaRESUMEN
Toxoplasma gondii is a nucleated cell obligate parasite, and can cause severe toxoplasmosis. The genetypes of Toxoplasma gondii isolates of different host infections have significant differences, and the pathogenicity and sensitivity to drugs of different genotypes of Toxoplasma gondii isolates are also significantly different. At present, the analysis of Toxoplasma gondii genotypes is performed by using PCR-RFLP, PCR of highly repetitive sequences, multiple PCR and nested PCR techniques, and the multiple loci of amplification are usually B1, SAG2, HSP70, GRA6 and other genetic markers. There are main 3 traditional genotypes of Toxoplasma gondii, and along with the deepening and extension of research, more and more genotypes are found. This paper reviews the advances in the research of Toxoplasma gondii genotypes.
Asunto(s)
ADN Protozoario/genética , Toxoplasma/genética , Animales , Genotipo , Humanos , Toxoplasmosis/parasitologíaRESUMEN
One hundred and fifty serum samples of HIV positive patients were collected in western Yunnan Province from September 2011 to December 2012. Toxoplasma gondii B1 gene was amplified by nested PCR. Genotyping of T. gondii isolates were performed by restriction fragment length polymorphism (RFLP) with Pm1 I and Xho I. 13 samples were found positive with the B1 gene (530 bp) amplification and belonged to type I. The sequencing results showed that 4 T. gondii B1 gene positive samples were identical, with 3 nucleotide variation compared with T. gondii strain RH (type I) B1 gene (GenBank No. AF179871), and in the other sample a "G --> A" mutation at 230bp was detected. The results indicated that the genotype of Toxoplasma gondii in HIV positive patients in Yunnan Province is type I.
Asunto(s)
ADN Protozoario/genética , Seropositividad para VIH/parasitología , Toxoplasma/genética , China/epidemiología , Genotipo , Seropositividad para VIH/epidemiología , Humanos , Reacción en Cadena de la Polimerasa , Toxoplasma/aislamiento & purificaciónRESUMEN
Different genotypes of Toxoplasma gondii show a great diversity in pathogenicity and drug sensitivity. Application of the PCR-derived technologies in gene identification and typing of T. gondii provides an important basis to clinical diagnosis and treatment. This article reviews the relevant technologies in gene identification and typing of T. gondii.
Asunto(s)
Reacción en Cadena de la Polimerasa/métodos , Toxoplasma/genética , Animales , ADN Protozoario/genética , Genotipo , Toxoplasma/clasificaciónRESUMEN
Serum samples were collected from HIV positive cases (927) and HIV, negative ones (80) from June 2010 to August 2011 in Dali and Dehong Prefectures of Yunnan. Serum anti-Toxoplasma gondii IgG was detected by ELISA. The overall anti-Toxoplasma gondii IgG positive rate among HIV positive cases and HIV negative ones was 35.1% (325/927) and 23.8% (19/80), respectively. In HIV positive cases, the seropositive rate was 30.3% (178/588) in Dali and 43.4% (147/339) in Dehong. The seropositive rate was significantly different among ethnic groups (chi2 = 28.433, P < 0.05). No significant difference was found among age groups (chi2 = 4.248, P > 0.05), and the age group of 41-60 showed the highest positive rate (36.1%, 103/285). The seropositive rate was 35.6% (203/571) in males and 34.3% (122/356) in females (chi2 = 0.158, P > 0.05).
Asunto(s)
Seropositividad para VIH/sangre , Seropositividad para VIH/parasitología , Toxoplasmosis/sangre , Adulto , Anticuerpos Antiprotozoarios/sangre , Pueblo Asiatico , China/epidemiología , Femenino , Infecciones por VIH/sangre , Infecciones por VIH/parasitología , Seropositividad para VIH/epidemiología , Humanos , Inmunoglobulina G/sangre , Masculino , Persona de Mediana Edad , Toxoplasma , Toxoplasmosis/epidemiología , Adulto JovenRESUMEN
A combination of Fructus psoraleae (3.4 g/kg) and dihydroartemisinin (60 mg/(kg x d)) was used for 7 d in mice infected with Cryptosporidium parvum infection induced by oral administration of dexamethasone. The results indicated that number of Cryptosporidium parvum oocysts in feces, proportion of CD4+, CD3+ T lymphocytes in blood, serum IFN-gamma, and nitric oxide (NO) content in intestinal tissue were all higher than those of infected control group (P < 0.01). The pathogenic change in intestine of treated mice was lighter than that of infected control. It indicates that the combination of Fructus psoraleae and dihydroartemisinin is effective to mice cryptosporidiosis probably through upregulating serum IFN-gamma, proportion of CD4+ and CD3+ T cells and NO concentration in intestine.