RESUMEN
Adipose tissue-derived stem cells (ADSCs) are an attractive source of mesenchymal stem cells (MSCs) for use in tissue engineering and clinical applications. This paper focuses on the characterization of ADSCs used as immunosuppressive agent in rabbits undergoing partial allograft for urine bladder restorage. For this study highlighted the characterization of the ADSCs used as immunosuppressive agents in rabbits submitted to partial allograft for restoration of the urinary vesicle, using 25 animals, six months old, New Zealand. ADSCs at the third peal were characterized by the MSC-specific CD105, CD73 and CD90 expression and by the absence of the hematopoietic marker CD45, as revealed by flow cytometry analysis. Moreover, ADSCs were efficient in preventing allograft rejection from the urinary bladder, as judged by biochemical, clinical and ultrasonography analysis. Together, these results compose characterization of protein expression profiles and immunosuppressive functionality of ADSCs in rabbits, which had undergone partial allografts of the urinary bladder, foreseeing future applications in clinical practice.(AU)
As células mesenquimais derivadas de tecido adiposo (ADSCs) são uma fonte atraente de células-tronco mesenquimais (MSCs) para uso na engenharia de tecidos e suas aplicações clínicas. Este trabalho destacou a caracterização das ADSCs utilizadas como agentes imunossupressores em coelhos submetidos a aloenxerto parcial para restauração da vesícula urinária, sendo utilizados 25 animais, de seis meses de idade, Nova Zelândia. As ADSCs, após o terceiro repique, foram caracterizadas pela expressão específica de MSC CD105, CD73 e CD90 e pela ausência do marcador hematopoiético CD45, tal como revelado por análise de citometria de fluxo. Além disso, os ADSCs foram eficientes na prevenção da rejeição de aloenxertos da vesícula urinária, conforme avaliado por análises clínica, bioquímica e ultrassonográfica. Juntos, esses resultados compõem a caracterização dos perfis de expressão proteica e a funcionalidade imunossupressora de ADSCs em coelhos, que sofreram aloenxertos parciais da bexiga, prevendo futuras aplicações na prática clínica.(AU)
Asunto(s)
Animales , Conejos , Conejos , Vejiga Urinaria/trasplante , Aloinjertos/citología , Tratamiento Basado en Trasplante de Células y Tejidos/veterinaria , Inmunosupresores , Citometría de Flujo/veterinariaRESUMEN
Adipose tissue-derived stem cells (ADSCs) are an attractive source of mesenchymal stem cells (MSCs) for use in tissue engineering and clinical applications. This paper focuses on the characterization of ADSCs used as immunosuppressive agent in rabbits undergoing partial allograft for urine bladder restorage. For this study highlighted the characterization of the ADSCs used as immunosuppressive agents in rabbits submitted to partial allograft for restoration of the urinary vesicle, using 25 animals, six months old, New Zealand. ADSCs at the third peal were characterized by the MSC-specific CD105, CD73 and CD90 expression and by the absence of the hematopoietic marker CD45, as revealed by flow cytometry analysis. Moreover, ADSCs were efficient in preventing allograft rejection from the urinary bladder, as judged by biochemical, clinical and ultrasonography analysis. Together, these results compose characterization of protein expression profiles and immunosuppressive functionality of ADSCs in rabbits, which had undergone partial allografts of the urinary bladder, foreseeing future applications in clinical practice.(AU)
As células mesenquimais derivadas de tecido adiposo (ADSCs) são uma fonte atraente de células-tronco mesenquimais (MSCs) para uso na engenharia de tecidos e suas aplicações clínicas. Este trabalho destacou a caracterização das ADSCs utilizadas como agentes imunossupressores em coelhos submetidos a aloenxerto parcial para restauração da vesícula urinária, sendo utilizados 25 animais, de seis meses de idade, Nova Zelândia. As ADSCs, após o terceiro repique, foram caracterizadas pela expressão específica de MSC CD105, CD73 e CD90 e pela ausência do marcador hematopoiético CD45, tal como revelado por análise de citometria de fluxo. Além disso, os ADSCs foram eficientes na prevenção da rejeição de aloenxertos da vesícula urinária, conforme avaliado por análises clínica, bioquímica e ultrassonográfica. Juntos, esses resultados compõem a caracterização dos perfis de expressão proteica e a funcionalidade imunossupressora de ADSCs em coelhos, que sofreram aloenxertos parciais da bexiga, prevendo futuras aplicações na prática clínica.(AU)
Asunto(s)
Animales , Conejos , Conejos , Vejiga Urinaria/trasplante , Aloinjertos/citología , Tratamiento Basado en Trasplante de Células y Tejidos/veterinaria , Inmunosupresores , Citometría de Flujo/veterinariaRESUMEN
This study compared acid-base and biochemical changes and quality of recovery in male cats with experimentally induced urethral obstruction and anesthetized with either propofol or a combination of ketamine and diazepam for urethral catheterization. Ten male cats with urethral obstruction were enrolled for urethral catheterization and anesthetized with either ketamine-diazepam (KD) or propofol (P). Lactated Ringer's solution was administered by intravenous (IV) beginning 15 min before and continuing for 48 h after relief of urethral obstruction. Quality of recovery and time to standing were evaluated. The urethral catheter was maintained to measure urinary output. Hematocrit (Hct), total plasma protein (TPP), albumin, total protein (TP), blood urea nitrogen (BUN), creatinine, pH, bicarbonate (HCO3-), chloride, base excess, anion gap, sodium, potassium, and partial pressure of carbon dioxide in mixed venous blood (pvCO2) were measured before urethral obstruction, at start of fluid therapy (0 h), and at subsequent intervals. The quality of recovery and time to standing were respectively 4 and 75 min in the KD group and 5 and 16 min in the P group. The blood urea nitrogen values were increased at 0, 2, and 8 h in both groups. Serum creatinine increased at 0 and 2 h in cats administered KD and at 0, 2, and 8 h in cats receiving P, although the values were above the reference range in both groups until 8 h. Acidosis occurred for up to 2 h in both groups. Acid-base and biochemical stabilization were similar in cats anesthetized with propofol or with ketamine-diazepam. Cats that received propofol recovered much faster, but the ketamine-diazepam combination was shown to be more advantageous when treating uncooperative cats as it can be administered by intramuscular (IM) injection.
Asunto(s)
Equilibrio Ácido-Base/efectos de los fármacos , Periodo de Recuperación de la Anestesia , Diazepam/farmacología , Ketamina/farmacología , Propofol/farmacología , Obstrucción Uretral/veterinaria , Acidosis , Anestesia Intravenosa/veterinaria , Anestésicos Disociativos/administración & dosificación , Anestésicos Disociativos/farmacología , Anestésicos Intravenosos/administración & dosificación , Anestésicos Intravenosos/farmacología , Animales , Nitrógeno de la Urea Sanguínea , Enfermedades de los Gatos/cirugía , Gatos , Creatinina/sangre , Diazepam/administración & dosificación , Quimioterapia Combinada , Hiperpotasemia , Ketamina/administración & dosificación , Masculino , Obstrucción Uretral/sangre , Obstrucción Uretral/cirugíaRESUMEN
OBJECTIVE: To compare the renal and cardiorespiratory effects of IV treatment with lactated Ringer's solution (LRS) or physiologic saline (0.9% NaCl) solution (PSS) in severely decompensated cats with urethral obstruction (UO). ANIMALS: 14 cats (4 cats were used only to establish infusion rates). PROCEDURES: An occluded urethral catheter was used to induce UO in each cat. After development of severe metabolic acidosis, hyperkalemia, and postrenal azotemia, the obstruction was relieved (0 hours); LRS or PSS (5 cats/group) was administered IV (gradually decreasing rate) beginning 15 minutes before and continuing for 48 hours after UO relief. Ten minutes before urethral catheter placement (baseline), at start of fluid therapy (SFT), and at intervals during fluid administration, various physical and clinicopathologic evaluations were performed. RESULTS: Metabolic acidosis was detected in the PSS-treated group at SFT and 2 hours after relief of UO and in the LRS-treated group only at SFT The PSS-treated group had significantly lower blood pH and bicarbonate concentrations at 8 through 48 hours and lower base excess values at 2 through 48 hours, compared with the LRS-treated group. Hypocalcemia and hypernatremia were detected in the PSS-treated group at 2 and 12 hours, respectively. Absolute serum potassium and chloride concentrations did not differ significantly between groups at any time point. CONCLUSIONS AND CLINICAL RELEVANCE: Treatment with LRS or PSS appeared to be safe and effective in cats with experimentally induced UO; however, LRS was more efficient in restoring the acid-base and electrolyte balance in severely decompensated cats with UO.
Asunto(s)
Enfermedades de los Gatos/tratamiento farmacológico , Soluciones Isotónicas/uso terapéutico , Riñón/fisiología , Cloruro de Sodio/uso terapéutico , Obstrucción Uretral/veterinaria , Animales , Temperatura Corporal/efectos de los fármacos , Enfermedades de los Gatos/fisiopatología , Gatos , Diuresis/efectos de los fármacos , Fluidoterapia/métodos , Fluidoterapia/veterinaria , Frecuencia Cardíaca/efectos de los fármacos , Riñón/efectos de los fármacos , Riñón/fisiopatología , Masculino , Orquiectomía , Lactato de Ringer , Albúmina Sérica/efectos de los fármacos , Albúmina Sérica/metabolismo , Obstrucción Uretral/tratamiento farmacológico , Obstrucción Uretral/fisiopatologíaRESUMEN
The purpose of this study was to determine the effect of cellulose membrane or free fat grafts (FFG) on laminectomy membrane (LM) formation. Eighteen dogs were randomly divided into three groups of six dogs. All dogs underwent a modified dorsal laminectomy on T(13)-L(1). The laminectomy defect was left uncovered in the control group but either a FFG or a cellulose membrane implant was provided in the other two groups. The dogs were evaluated through neurological examination, myelography, macroscopic roundness index of spinal cord and histological evaluations of epidural fibrosis and spinal cord. The results showed a significant difference between the control and the FFG group, with the FFG causing neurological deficits and spinal cord compression as assessed by the roundness index of the spinal cord. Both FFG and cellulose membrane were partially effective in preventing LM formation. The use of FFG was associated with a high rate of significant neurological complications and spinal cord lesions.
Asunto(s)
Enfermedades de los Perros/cirugía , Laminectomía/veterinaria , Enfermedades de la Columna Vertebral/veterinaria , Mallas Quirúrgicas , Tejido Adiposo , Animales , Celulosa , Enfermedades de los Perros/patología , Perros , Laminectomía/métodos , Mielografía/veterinaria , Complicaciones Posoperatorias/veterinaria , Prótesis e Implantes/veterinaria , Distribución Aleatoria , Médula Espinal/patología , Médula Espinal/cirugía , Compresión de la Médula Espinal/etiología , Compresión de la Médula Espinal/patología , Compresión de la Médula Espinal/cirugía , Compresión de la Médula Espinal/veterinaria , Enfermedades de la Columna Vertebral/patología , Enfermedades de la Columna Vertebral/cirugíaRESUMEN
A catarata é reconhecida como qualquer opacificação da lente e seu tratamento é cirúrgico. A pressão intra-ocular é dada pelo equilíbrio entre a entrada e saída de humor aquoso nas câmaras anterior e posterior do olho. Este artigo traz uma revisão a respeito das implicações da catarata e de seu tratamento cirúrgico sobre a pressão intra-ocular, bem como discute os métodos indicados para controle e manutenção da pressão intra-ocular em cães.
ABSTRACT: Cataract is a disease recognized as any lens opacifi cation and its treatment is surgical. The intra-ocular pressure is given by the balance between the entrance and the exit of aqueous humor in the anterior and posterior chambers of the eye. This article presents a review about the implications of cataract and its surgical treatment on the intra-ocular pressure, as well as it discuss the indicated methods for control and maintenance of the intra-ocular pressure in dogs.
RESUMEN: La catarata es una enfermedad reconocida como cualquier opacidad del cristalino, y su tratamiento es quirúrgico. La presión intraocular es debida al equilibrio entre la entrada y la salida del humor acuoso en las cámaras anterior y posterior del ojo. Este artículo presenta una revisión a respecto de las implicaciones de la catarata y de su tratamiento quirúrgico sobre la presión intraocular, así como discute los métodos para control y manutención de la presión intraocular en perros.
Asunto(s)
Presión Intraocular , Catarata/fisiopatología , Catarata/veterinaria , Cirugía Veterinaria/métodos , Facoemulsificación/métodos , Facoemulsificación/veterinaria , Oftalmopatías/cirugía , Oftalmopatías/complicaciones , Glaucoma/complicaciones , Uveítis/complicacionesRESUMEN
A catarata é reconhecida como qualquer opacificação da lente e seu tratamento é cirúrgico. A pressão intra-ocular é dada pelo equilíbrio entre a entrada e saída de humor aquoso nas câmaras anterior e posterior do olho. Este artigo traz uma revisão a respeito das implicações da catarata e de seu tratamento cirúrgico sobre a pressão intra-ocular, bem como discute os métodos indicados para controle e manutenção da pressão intra-ocular em cães.(AU)
Cataract is a disease recognized as any lens opacifi cation and its treatment is surgical. The intra-ocular pressure is given by the balance between the entrance and the exit of aqueous humor in the anterior and posterior chambers of the eye. This article presents a review about the implications of cataract and its surgical treatment on the intra-ocular pressure, as well as it discuss the indicated methods for control and maintenance of the intra-ocular pressure in dogs.(AU)
La catarata es una enfermedad reconocida como cualquier opacidad del cristalino, y su tratamiento es quirúrgico. La presión intraocular es debida al equilibrio entre la entrada y la salida del humor acuoso en las cámaras anterior y posterior del ojo. Este artículo presenta una revisión a respecto de las implicaciones de la catarata y de su tratamiento quirúrgico sobre la presión intraocular, así como discute los métodos para control y manutención de la presión intraocular en perros.(AU)
Asunto(s)
Presión Intraocular/fisiología , Catarata/fisiopatología , Catarata/veterinaria , Cirugía Veterinaria/métodos , Facoemulsificación/métodos , Facoemulsificación/veterinaria , Oftalmopatías/complicaciones , Oftalmopatías/cirugía , Glaucoma/complicaciones , Uveítis/complicacionesRESUMEN
The present study was designed to investigate the effects of nickel chloride on dietary iron deficiency in rats. The gree of iron deficiency was relatively moderate, but a more generalized anemia occurred in iron deficiency, in absence of nickel chloride. Moderate iron deficiency anemia induced increased lactate-dehydrogenase activity of serum and bone marrow, perhaps related to the decreased production of energy by oxidative means, Nickel chloride, perhaps for its ability to change iron absorption, for the maintenance of bone marrow metabolism and for to increase ceruloplasmin activity, inhibited the alteration on hemoglobin synthesis. Furthermore, nickel chloride possibly for its action on copper content and Cu-Zn superoxide-dismutase activity, inhibits the shortening of the red cell life span, caused by superoxide radicals
Asunto(s)
Ratas , Animales , Cloruros/efectos adversos , Níquel , Anemias NutricionalesRESUMEN
Alteraçöes no peso corporal, hamoglobina, proteina sérica, ferro, fóforo e em atividades de enzizmas no soro e medula óssea foram investigadas em ratos consumindo uma dieta carente em ferro, em presença e ausencia de cloreto de niqueo (NiCl2). O grau de deficiencia em ferro, no presente trabalho foi suficiente para induzir anemia moderada, sem produzir alteraçöes nas atividade da desidrogenase-láctica total e suas isoenzimas no cérebro de ratos. Anemia moderada ocorreu somente em ratos deficientes em ferro em ausencia de cloreto de níquel. Moderada anemia ferropriva induziu elevaçäo nas actividades da desidrogenase-láctica na medula óssea, provavelmente devido a diminuiçäo na produçäo de energia através de mecanismos oxidativos. Cloreto de níquel, aparentemente por sua capacidade de alterara absorçäo de ferro e pala manutençäo do metabolismo da medula ósseas, inibiu as alteraçöes na biosíntese de hemoglobina e nas atividades da desidrogenase-láctica da medula óssea de ratos deficientes em ferro
Asunto(s)
Ratas , Animales , Masculino , /fisiopatología , Hemoglobinuria/efectos de los fármacos , /metabolismo , L-Lactato Deshidrogenasa/metabolismo , L-Lactato Deshidrogenasa/farmacología , Médula Ósea , Médula Ósea/enzimología , Médula Ósea/metabolismo , Níquel/farmacología , Níquel/metabolismoRESUMEN
Aloxana é amplamente utilizada em estudos de diabetes experimental, porque destroi as células pancreáticas produtoras de insulina, com relativa seletividade. Evidências recentes indicam que superóxido-dismutase (E.C.1.15.1.1.) e inhibidores de radicais hidroxil, protegem as células pancreáticas aos efeitos tóxicos de aloxana. No presente trabalho, os autores estudaram os efeitos do cloreto de níquel e da aloxana sobre as concentraçöes de calcio, zinci, fósforo e ferro. Foi observado que cloreto de níquel induziu elevaçäo nos conteúdos de calcio e zinco no pancreas de ratos tratados com aloxana. O possível efeito dos cloreto de níquel sobre a açäo de aloxana, deve estar relacionado com o efeito do níquel sobre a actividade da superóxidodismutase, mediada pelo zinco e plo cobere