RESUMEN

This analysis has two interlinked goals. First, drawing on Lee Edelman, Dudley Andrew and Laura Mulvey, it theorizes the male homosexual optique, a mode of address that is predicated on the relationship between a film, its context of reception and its spectator, and which undermines the heterosexual exigencies of classical narrative style to implicitly address a gay male viewer. Second, it illustrates how the putatively heterosexual romance of Curtis Harrington's Night Tide (1961) appeals to gay male spectators through two mechanisms that underpin the optique, specifically the homosexual gaze and characterization. Key to each of these aims is how the non-normative heterosexual male body functions as a site of gay male objectification and identification. This article ultimately argues that Harrington's film enables us to envisage a queer cinema that is not predicated on fictions of homosexual desire but nevertheless provides a framework for deepening our understanding of what queer spectatorship (and the male homosexual gaze in particular) may involve.

Asunto(s)

Minorías Sexuales y de Género , Humanos , Masculino , Homosexualidad Masculina , Identidad de Género , Películas Cinematográficas , Heterosexualidad

RESUMEN

Preventing peritoneal implantation of carcinoma cells could prolong ovarian cancer patient remission and survival. Peritoneal cells constitutively express mesothelin, a ligand for CA125 that is expressed by tumor cells. Thus blocking CA125/mesothelin-dependent cell attachment may prevent or delay peritoneal metastatic recurrence. We developed a high-throughput screening system for reagents able to block CA125/mesothelin-dependent cell attachment with a sensitive quantitative readout. Using a novel yeast-expression system to produce secreted, in vivo biotinylated proteins and biobodies (Bbs), we generated anti-mesothelin Bbs. Anti-mesothelin Bbs derived from high affinity yeast-display scFv detected both membrane-bound and soluble mesothelins and inhibited CA125/mesothelin-dependent cell attachment.

Asunto(s)

Anticuerpos Monoclonales/biosíntesis , Anticuerpos Monoclonales/farmacología , Antígeno Ca-125/efectos de los fármacos , Adhesión Celular/efectos de los fármacos , Glicoproteínas de Membrana/antagonistas & inhibidores , Neoplasias Peritoneales/prevención & control , Secuencia de Aminoácidos , Anticuerpos Monoclonales/genética , Bioensayo , Diploidia , Ensayos de Selección de Medicamentos Antitumorales/métodos , Proteínas Ligadas a GPI , Humanos , Región Variable de Inmunoglobulina/biosíntesis , Región Variable de Inmunoglobulina/genética , Región Variable de Inmunoglobulina/farmacología , Mesotelina , Datos de Secuencia Molecular , Neoplasias Peritoneales/secundario , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/genética , Proteínas Recombinantes/farmacología , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo