RESUMEN
It is established that after the termination of deep saturation dives reorganization of respiratory system at navy aquanauts proceeds. This remodeling is specific, atypical for natural age dynamics.
Asunto(s)
Remodelación de las Vías Aéreas (Respiratorias) , Buceo/efectos adversos , Enfermedades Pulmonares/etiología , Personal Militar , Remodelación de las Vías Aéreas (Respiratorias)/fisiología , Buceo/fisiología , Humanos , Enfermedades Pulmonares/diagnóstico , Enfermedades Pulmonares/diagnóstico por imagen , Enfermedades Pulmonares/fisiopatología , Medicina Naval , Cintigrafía , Pruebas de Función Respiratoria , Factores de TiempoRESUMEN
Using flow cytometry and radioimmunological test, a study was made of cytotoxic activities of natural killer (NK) cells of human peripheral blood of three patient groups: healthy donors, persons with infection pathology, and persons being in extreme situations (hyperoxygen gas mixture). With the increase in effector and target (K562) cell amount ratio, NK activity was seen elevating in all groups of patients examined. A correlation was obtained between NK activity in healthy donors and in patients examined by both the methods. NK activity in sick patients, registered by the two methods, was considerably lower than in healthy donors. NK activity in persons that appeared in extreme situations, registered by the radioimmunological test only, did not differ from that in healthy donors, whereas NK activity, registered by the flow cytometry, was found to be considerably lower, resp.
Asunto(s)
Células Asesinas Naturales/inmunología , Donantes de Sangre , Citotoxicidad Inmunológica , Citometría de Flujo/métodos , Humanos , Hiperoxia/inmunología , Infecciones/inmunología , Inflamación/inmunología , Radioinmunoensayo/métodosRESUMEN
The ability of monoclonal antibodies (MAb) to HNK1 antigen of natural killers (NK), previously purified by affinity chromatography, to react with human blood lymphocytes has been studied by flow cytometry. The results obtained point to some cells, among blood lymphocytes, which have antigenic determinants to these MAbs. A decrease in the NK cell cytotoxic activity was shown after addition of MAb in comparison with the control. This enables us to suggest a reactivity of these MABs to the CD57 leucocyte differential antigens.
Asunto(s)
Anticuerpos Monoclonales/inmunología , Antígenos CD/inmunología , Antígenos de Diferenciación de Linfocitos T/inmunología , Células Asesinas Naturales/inmunología , Animales , Anticuerpos Monoclonales/análisis , Anticuerpos Monoclonales/aislamiento & purificación , Antígenos CD57 , Cromatografía de Afinidad , Pruebas Inmunológicas de Citotoxicidad , Ensayo de Inmunoadsorción Enzimática , Citometría de Flujo , Técnica del Anticuerpo Fluorescente , Humanos , Hibridomas/inmunología , Inmunoglobulina M/aislamiento & purificación , Ratones , Ratones Endogámicos BALB CRESUMEN
The complex investigation of immune and nonspecific reactivity of 30 aquanauts was carried out during five experimental saturation divings up to 350-500 m accompanied by an increase of microbic contamination in water of the hyperbaric chamber. Peculiarities of humoral immune response and phagocyte functions were found to depend on the inflammatory disease of aquanauts. It is concluded that the situational transitory immune deficiency development under the influence of hyperbaric factors is possible and changes in the microbe spectrum are real.