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Sulphide oxidising bacteria (SOB) have the potential to be used for bioelectrochemical removal, i.e. oxidation, of sulphide from waste streams. In anaerobic conditions, SOB are able to spatially separate sulphide removal and terminal electron transfer to an electrode and act as a sulphide shuttle. However, it is not fully understood how SOB anaerobically remove sulphide and store charge equivalents, and where in this process sulphur is formed. Therefore, the redox behaviour of sulphide shuttling SOB was investigated at haloalkaline conditions using a glassy carbon rotating disc electrode (RDE) and cyclic voltammetry. Voltammograms of SOB in the absence and presence of sulphide were compared to voltammograms of abiotic sulphur species solutions. Polysulphide and sulphide showed different redox behaviour, with distinct potentials for oxidation of > -0.3 V (vs. Ag/AgCl) for polysulphide and > -0.1 V for sulphide. Comparing biotic to abiotic experiments lead to the hypothesis that SOB formed polysulphides during anaerobic sulphide removal, which stayed sorbed to the cells. With this study, further steps were taken in elucidating the mechanisms of sulphide shuttling by SOB.

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Mixtures of n-carboxylic acids (n-CA) as derived from microbial conversion of waste biomass were converted to bio-fuel using Kolbe electrolysis. While providing full carbon and electron balances, key parameters like electrolysis time, chain length of n-CA, and pH were investigated for their influence on reaction efficiency. Electrolysis of n-hexanoic acid showed the highest coulombic efficiency (CE) of 58.9±16.4 % (n=4) for liquid fuel production among individually tested n-CA. Duration of the electrolysis was varied within a range of 0.27 to 1.02 faraday equivalents without loss of efficiency. Noteworthy, CE increased to around 70 % by hetero-coupling when electrolysing n-CA mixtures regardless of the applied pH. Thus, 1 L of fuel could be produced from 12.4 mol of n-CA mixture using 5.02 kWh (<1 € L-1 ). Thus, a coupling with microbial processes producing n-CA mixtures from different organic substrates and waste is more than promising.

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Five commercial materials were assessed for electrochemical conversion of n-hexanoic acid by Kolbe electrolysis. Platinized titanium performed best, achieving a coulombic efficiency (CE) of 93.1±6.7 % (n=6) for the degradation of n-hexanoic acid and 48.3±3.2 % (n=6) for the production of n-decane, which is close to the performance of pure platinum (89.7±14.4 and 55.5±3.5 %; n=6). 56.7 mL liquid fuel was produced per mole n-hexanoic acid, converting to an energy demand of 6.66 kWh and 1.22 € per L. Using optical profilometry and scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy, it was shown that the degree of coverage of the titanium surface with platinum played the most important role. An uncovered surface of as little as 1-3 % already led to a deterioration of the CE of approximately 50 %. Using platinized titanium requires >36 times less capital expenditure at only <10 % increased operational expenditure; an electrode lifetime of 10000 h can be expected.

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Power-to-X technologies have the potential to pave the way towards a future resource-secure bioeconomy as they enable the exploitation of renewable resources and CO2 . Herein, the coupled electrocatalytic and microbial catalysis of the C5 -polymer precursors mesaconate and 2S-methylsuccinate from CO2 and electric energy by in situ coupling electrochemical and microbial catalysis at 1 L-scale was developed. In the first phase, 6.1±2.5 mm formate was produced by electrochemical CO2 reduction. In the second phase, formate served as the substrate for microbial catalysis by an engineered strain of Methylobacterium extorquens AM-1 producing 7±2 µm and 10±5 µm of mesaconate and 2S-methylsuccinate, respectively. The proof of concept showed an overall conversion efficiency of 0.2 % being 0.4 % of the theoretical maximum.

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For some applications of bioplastics like food packaging or medical devices, applying additives can be necessary to avoid microbial activity and hinder biofilm or fouling formation. A currently promising additive is chitosan (CS), the deacetylated form of the biogenic scaffolding material chitin. Due to its hydrophilicity, chitosan is not compatible with most of the thermoplastic bio-based polymers like poly(lactic acid) (PLA) or polyhydroxyalkanoates (PHA). In this work, compatibilization between chitosan and two selected bio-based polyesters, PLA and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), was enhanced by grafting maleic anhydride (MAH) and glycidyl methacrylate (GMA), respectively, onto polymer chains using peroxide. The success of grafting was confirmed via titration methods. The effects of grafting agent and peroxide concentrations on grafting reaction and the physical and thermal properties of the functionalized polyesters were investigated. Compounding of the functionalized polyesters with different weight portions of chitosan was accomplished in a discontinuous internal mixer by in-situ functionalization, followed by blending with chitosan. The titration method, scanning electron microscopy, DSC, FTIR and mechanical characterization of the composites showed good interfacial adhesion and suggest the formation of covalent bonds between functional groups of the polyesters and chitosan, especially for the samples functionalized with GMA. The molecular weights (Mw) of the samples showed a change in the molecular weight related to the thermal degradation of the sample. The Mw of the samples grafted with MAH are lower than those functionalized with GMA. Furthermore, integration of chitosan into non-functionalized PLA polymer matrix showed a nucleating effect, while for PHBV, the increase of crystallinity with the content of chitosan was only observed for grafted PHBV.

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BACKGROUND: The frequency of caesarean section has increased dramatically in recent decades. Despite this, robust data regarding the consequences of caesarean section in terms of developing chronic postsurgical pain (CPSP) are still lacking. OBJECTIVE: This systematic review analysed the incidence and severity of CPSP in women 3 to less than 6, 6 to less than 12, and at least 12 months after caesarean section. DESIGN: Systematic review of prospective and retrospective observational studies and randomised controlled trials with meta-analysis. DATA SOURCE: We searched MEDLINE to May 2015. ELIGIBILITY CRITERIA: We included all studies investigating the incidence and/or severity of CPSP at least 3 months after caesarean section. The primary outcome was chronic postsurgical wound pain (CPSP 'wound'). Secondary outcomes were persistent pain in the back area, pelvic region or reported as residual pain, and severity of 'birth-related' chronic pain. RESULTS: Meta-analysis using the random-effects model based on 15 studies (n = 4475) reporting CPSP 'wound' at 3 to less than 6 months after caesarean section revealed an incidence of 15.4% [95% confidence interval (CI): 9.9 to 20.9%]. For 6 to less than 12 and at least 12 months after caesarean section, the incidence of CPSP 'wound' was estimated at 11.5% (95% CI: 8.1 to 15.0%, n = 3345) and 11.2% (95% CI: 7.4 to 15.0%, n = 3451), respectively. Meta-regression analysis using the publication year as predictor revealed stable CPSP 'wound' incidences at each postoperative time slot from 2002 to the present. Of those patients who reported chronic pain, 9.6% (95% CI: 0.0 to 21.0%) had severe pain, 23.5% (95% CI: 10.0 to 37.0%) had moderate pain and 49.2% (95% CI: 18.9 to 79.4%) had mild pain at 6 months. LIMITATIONS: Major limitations are high statistical heterogeneity of the meta-analyses and inconsistencies in reporting severity of chronic 'birth-related' pain. CONCLUSION: This meta-analysis finds a clinically relevant incidence of CPSP 'wound' after caesarean section ranging from 15% at 3 months to 11% at 12 months or longer that has been largely stable in recent years.

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