RESUMEN
The sociological survey was organized for developing of organization of medical care to socially deadapted patients in rural territories. The sampling consisted of 156 district therapists of central district hospitals of the Novosibirskaia oblast providing primary medical care at out-patient stage. The females consisted 83.3%, males 16.7°%, average age of respondents made up to 43 years. The carrier experience made up to 19 years in average. It is established that in 57.1% of cases patients indicated social factors as a cause of development of disease. At that, 82.7°% of physicians are confident that activities concerning social adaptation of socially deadapted patients will ameliorate quality of provided medical care. The most of respondents (64.15) indicate the need in activities concerning social adaptation of such patients. The most of physicians (67.2%) mention that activities of adaptation of socially deadapted patients are to involve clinical psychologists and psychotherapists and 31.7°% consider that it is necessary to involve social workers. The organization of rehabilitation activities of social adaptation for this type of contingent is to be intersectoral with participation of medical and social workers.
Asunto(s)
Atención a la Salud/estadística & datos numéricos , Pacientes Ambulatorios/estadística & datos numéricos , Atención Primaria de Salud/estadística & datos numéricos , Población Rural/estadística & datos numéricos , Ajuste Social , Adulto , Atención a la Salud/normas , Femenino , Humanos , Masculino , Persona de Mediana Edad , Siberia/epidemiologíaRESUMEN
The RT-PCR method with real-time fluorescence detection was used for development of φ prototype of diagnostic kit for reliable diagnosis of genetic variants of RNA of the HIV-1 of groups M, N, O, P and RNA of the HIV-2 in blood plasma and serum. The kit is stable against nucleotide defects, provides broad linear range of concentration of the HIV RNA, 100% analytical specificity and adequate analytical sensitivity: 42 ME/ml (HIV-1 of group M), 45 copies/ml (HIV-2), 92 copies/ml (HIV-1 of group O), 90 copies/ml (HIV-1 of group N). The kit provided successful detection and measurement of HIV RNA concentration in the samples of the international reference panel of the HIV-1 genotype. The results of the test correlate with results of commercial tests.
Asunto(s)
Infecciones por VIH/genética , VIH-1/genética , VIH-2/genética , Reacción en Cadena en Tiempo Real de la Polimerasa/métodos , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Variación Genética , Infecciones por VIH/diagnóstico , Seropositividad para VIH/genética , VIH-1/aislamiento & purificación , VIH-2/aislamiento & purificación , Humanos , ARN Viral/genética , ARN Viral/aislamiento & purificaciónRESUMEN
To decrease dependence of effectiveness of isolation of nucleic acids of composition and amount of applied sample a kit was developed for hybridization extraction of DNA HBV RNA HCV and RNA HIV from blood serum in two formats--using up to 250 mkl and up to 1 ml of sample. This kit, in complex with kits for detection using polymerase chain reaction technique in real-time, forms a test characterized by high analytical sensitivity i.e. HBV50 copies per ml, HCV37.5 copies per ml, HIV 13 copies per ml. The developed kit for extraction of target nucleic acids permits to get rid of total DNA and inhibited effect of heparin. It can be adapted for application wit factors B and automated stations of sample preparation.