Asunto(s)
Gripe Humana/prevención & control , Animales , Anticuerpos Antivirales/sangre , Antígenos Virales/sangre , Evolución Biológica , Glicoproteínas Hemaglutininas del Virus de la Influenza/genética , Humanos , Virus de la Influenza A/genética , Virus de la Influenza A/inmunología , Gripe Humana/epidemiología , Gripe Humana/virología , JapónRESUMEN
An influenza B virus, B/Saga/S172/99 (SAG99), was isolated from the nasopharynx of a patient with encephalopathy/encephalitis in Japan in 1999. To clarify the molecular characteristics of this virus, detailed analysis of the gene segments coding for the hemagglutinin (HA), neuraminidase (NA), nucleoprotein (NP), matrix protein (M) and non-structural protein (NS) was undertaken. All five genes of SAG99 showed high nucleotide and predicted amino acid similarities with those of recent non-encephalopathic strains isolated in the same epidemic season. Subsequent phylogenetic analysis revealed that all five gene segments of SAG99 analyzed in the present study were most similar to those of the recent Yamagata/16/88-like viruses. The hemagglutinin and neuraminidase proteins of SAG99 were each distinguished from those of recent epidemic strains by one characteristic amino acid substitution. These substitutions were not found in the previously reported encephalopathy/encephalitis-derived influenza B viruses, and we could not find any common characteristic amino acid changes in SAG99 and these viruses. Similarly, among the internal proteins studied, only the M2 protein of SAG99 was found to contain a single novel amino acid change when compared with other recent isolates. Thus, it was apparent that SAG99 contained very few amino acid differences when compared with other epidemic viruses. The association of recent B/Yamagata/16/88-like viruses with encephalitis/encephalopathy observed in the present study and previously suggest that these viruses may have a higher potential for causing neurological complications in certain individuals.
Asunto(s)
Encefalitis Viral/virología , Virus de la Influenza B/clasificación , Virus de la Influenza B/genética , Gripe Humana/complicaciones , Proteínas Virales/genética , Niño , Femenino , Genes Virales , Humanos , Virus de la Influenza B/aislamiento & purificación , Gripe Humana/virología , Datos de Secuencia Molecular , Filogenia , Análisis de Secuencia de ADNRESUMEN
In Shizuoka Prefecture, influenza B viruses belonging to B/Victoria/2/87 lineage caused an outbreak among school children in the off-season of 1998, and the same B viruses were mainly isolated during the following epidemic. Low titer of HI antibody among children for influenza B virus belonging to the same lineage was a recognized factor in the causation of the distinctive epidemic. The herald virus strains seemed to be antigenically similar to the late epidemic virus strains, but the former strains were not genetically close to the latter. This indicates that the herald viruses are not always the parental viruses for the following influenza season.
Asunto(s)
Virus de la Influenza B/aislamiento & purificación , Gripe Humana/epidemiología , Gripe Humana/virología , Niño , Brotes de Enfermedades , Humanos , Japón/epidemiología , Estaciones del AñoRESUMEN
The entire nucleotide sequences of all six internal genes of six human H5N1 influenza A viruses isolated in Hong Kong in 1997 were analysed in detail from a phylogenetic point of view and compared with the evolutionary patterns of the haemagglutinin and neuraminidase genes. Despite being isolated within a single year in the same geographical location, human H5N1 viruses were characterized by a variety of amino acid substitutions in the ribonucleoprotein complex [PB2, PB1, PA and nucleoprotein (NP)] as well as the matrix (M) proteins 1 and 2 and nonstructural (NS) proteins 1 and 2. The presence of previously reported amino acid sequences specific for human strains was confirmed in the PB2, PA, NP and M2 proteins. Nucleotide and amino acid sequence identities of the six internal genes of H5N1 viruses examined here were separated into at least two variant groups. In agreement with the above result, phylogenetic trees of the six internal genes of human H5N1 viruses were generally composed of two minor clades. Additionally, variable dendrogram topologies suggested that reassortment among viruses contributed further to the genetic variability of these viruses. As a result, it became clear that human H5N1 viruses are characterized by divergent gene constellations, suggesting the possible occurrence of genetic reassortment between viruses of the two evolutionary lineages.
Asunto(s)
Evolución Molecular , Genes Virales , Variación Genética , Subtipo H5N1 del Virus de la Influenza A , Virus de la Influenza A/genética , Nucleoproteínas , ARN Polimerasa Dependiente del ARN , Proteínas Virales/genética , ARN Polimerasas Dirigidas por ADN/química , ARN Polimerasas Dirigidas por ADN/genética , Humanos , Datos de Secuencia Molecular , Proteínas de la Nucleocápside , Filogenia , Análisis de Secuencia de ADN , Proteínas del Núcleo Viral/química , Proteínas del Núcleo Viral/genética , Proteínas de la Matriz Viral/química , Proteínas de la Matriz Viral/genética , Proteínas no Estructurales Virales/química , Proteínas no Estructurales Virales/genética , Proteínas Virales/químicaRESUMEN
Phylogenetic patterns of the three polymerase (PB2, PB1 and PA) genes of a total of 20 influenza B viruses isolated during a 58 year period, 1940-1998, were analysed in detail in a parallel manner. All three polymerase genes consistently showed evolutionary divergence into two major distinct lineages and their amino acid profiles demonstrated conserved lineage-specific substitutions. Dendrogram topologies of the PB2 and PB1 genes were very similar and contrasted with that of the PA gene. It was of particular interest to reveal that even though the PA gene evolved into two major lineages, that of three recent Asian Victoria/1/87-like strains formed a branch cluster located in the same lineage as that of recent Yamagata/16/88-like isolates. Differences in the phylogenetic pathways of three polymerase genes were not only a reflection of genetic reassortment between co-circulating influenza B viruses, but also an indication that the polymerase genes were not co-evolving as a unit. As a result, comparison of the phylogenetic patterns of the three polymerase genes with previously determined patterns of the HA, NP, M and NS genes of 18 viruses defined the existence of eight distinct genome constellations. Also, similar phylogenetic profiles among the PA, NP and M genes, as well as between the PB2 and PB1 genes, were observed, suggesting possible functional interactions among these proteins. Completion of evolutionary analysis of the six internal genes and the HA gene of influenza B viruses revealed frequent genetic reassortment among co-circulating variable strains and suggested co-dependent evolution of genes.
Asunto(s)
Genes Virales , Virus de la Influenza B/genética , ARN Polimerasa Dependiente del ARN/genética , Proteínas Virales/genética , Secuencia de Aminoácidos , Datos de Secuencia Molecular , FilogeniaRESUMEN
This study was initiated with the isolation of influenza A and B viruses from clinical throat swabs in both fertile chicken eggs (egg) and MDCK cells, which were used in subsequent vaccine production in the above two hosts. On the basis of haemagglutination-inhibiting (HI) tests, immune mouse sera from mice vaccinated with MDCK cell-derived vaccines revealed antigenic similarities among H3N2 or B viruses isolated in MDCK cells or eggs. Similarly, antiserum prepared by immunization with egg-derived H3N2 vaccine showed equivalent antigenicity between homologous and heterologous (MDCK cell-derived) viruses. In contrast, antigenicity of egg-derived B vaccines was differed somewhat from that of MDCK cell-derived vaccines, suggesting the occurrence of antigenic change due to passaging in eggs. The time-course of immune responses based on HI titres indicated that MDCK cell-derived vaccines elicited extremely high antibody levels. Also, it was evident that antibody production by MDCK cell-grown H3N2 vaccine was very similar to that of vaccine prepared from egg-grown viruses. These results were comparable to those of plaque neutralization tests, although antigenic differences between egg- and MDCK cell-derived challenge viruses were confirmed in the test with antiserum to MDCK cell-derived vaccine. Consistent with HI-antibody production, the immunogenicity of MDCK cell-derived B vaccine appeared to be low by plaque neutralization test, while immune responses in mice which received egg-derived vaccines were significantly higher than that of the former. Furthermore, immune responses confirmed in mice immunized with B virus vaccines prepared in eggs revealed slight antigenic differences between two viruses derived from their respective hosts. Nevertheless, through evaluation of immune responses, MDCK cell-derived influenza vaccines may be useful when weak immunogenicity of B virus vaccine is improved.
Asunto(s)
Virus de la Influenza A/inmunología , Virus de la Influenza B/inmunología , Vacunas contra la Influenza/inmunología , Cultivo de Virus , Animales , Anticuerpos Antivirales/inmunología , Antígenos Virales/inmunología , Línea Celular , Embrión de Pollo , Perros , Estudios de Evaluación como Asunto , Pruebas de Inhibición de Hemaglutinación , Humanos , Ratones , Modelos Inmunológicos , Pruebas de Neutralización , Vacunas de Productos Inactivados/inmunologíaRESUMEN
Phylogenetic profiles of the genes coding for the hemagglutinin (HA) protein, nucleoprotein (NP), matrix (M) protein, and nonstructural (NS) proteins of influenza B viruses isolated from 1940 to 1998 were analyzed in a parallel manner in order to understand the evolutionary mechanisms of these viruses. Unlike human influenza A (H3N2) viruses, the evolutionary pathways of all four genes of recent influenza B viruses revealed similar patterns of genetic divergence into two major lineages. Although evolutionary rates of the HA, NP, M, and NS genes of influenza B viruses were estimated to be generally lower than those of human influenza A viruses, genes of influenza B viruses demonstrated complex phylogenetic patterns, indicating alternative mechanisms for generation of virus variability. Topologies of the evolutionary trees of each gene were determined to be quite distinct from one another, showing that these genes were evolving in an independent manner. Furthermore, variable topologies were apparently the result of frequent genetic exchange among cocirculating epidemic viruses. Evolutionary analysis done in the present study provided further evidence for cocirculation of multiple lineages as well as sequestering and reemergence of phylogenetic lineages of the internal genes. In addition, comparison of deduced amino acid sequences revealed a novel amino acid deletion in the HA1 domain of the HA protein of recent isolates from 1998 belonging to the B/Yamagata/16/88-like lineage. It thus became apparent that, despite lower evolutionary rates, influenza B viruses were able to generate genetic diversity among circulating viruses through a combination of evolutionary mechanisms involving cocirculating lineages and genetic reassortment by which new variants with distinct gene constellations emerged.
Asunto(s)
Evolución Molecular , Genes Virales , Glicoproteínas Hemaglutininas del Virus de la Influenza/genética , Virus de la Influenza B/genética , Nucleoproteínas , Proteínas del Núcleo Viral/genética , Proteínas de la Matriz Viral/genética , Proteínas no Estructurales Virales/genética , Secuencia de Bases , ADN Viral , Humanos , Virus de la Influenza B/clasificación , Virus de la Influenza B/aislamiento & purificación , Datos de Secuencia Molecular , Proteínas de la Nucleocápside , FilogeniaRESUMEN
Nucleotide sequences of all eight RNA segments of 10 human H3N2 influenza viruses isolated during a 5-year period from 1993 to 1997 were determined and analyzed phylogenetically in order to define the evolutionary pathways of all genes in a parallel fashion. It was evident that the hemagglutinin and neuraminidase genes of these viruses evolved essentially in a single lineage and that amino acid changes accumulated sequentially with respect to time. In contrast, amino acid differences in the internal proteins were erratic and did not accumulate over time. Parallel analysis of the phylogenetic patterns of all genes revealed that the evolutionary pathways of the six internal genes were not linked to the surface glycoproteins. Genes coding for the basic polymerase-1, nucleoprotein, and matrix proteins of 1997 isolates were closest phylogenetically to those of earlier isolates of 1993 and 1994. Furthermore, all six internal genes of four viruses isolated in the 1995 epidemic season consistently divided into two distinct branch clusters, and two 1995 isolates contained PB2 genes apparently originating from those of viruses before 1993. It was apparent that the lack of correlation between the topologies of the phylogenetic trees of the genes coding for the surface glycoproteins and internal proteins was a reflection of genetic reassortment among human H3N2 viruses. This is the first evidence demonstrating the occurrence of genetic reassortment involving the internal genes of human H3N2 viruses. Furthermore, internal protein variability coincided with marked increases in the activity of H3N2 viruses in 1995 and 1997.
Asunto(s)
Genoma Viral , Subtipo H3N2 del Virus de la Influenza A , Virus de la Influenza A/genética , Filogenia , Secuencia de Aminoácidos , Evolución Molecular , Japón , Datos de Secuencia Molecular , Proteínas de la Matriz Viral/genéticaRESUMEN
New antigenic variants of B/Yamagata/16/88-like lineage which appeared in the season of 1997 as a minor strain tended to predominate in the following season. Also, we could observe for the first time, three peaks of activity caused by H3N2 virus and two variants of B influenza virus. Antigenic and phylogenetic analyses revealed that B/Victoria/2/87-like variants appeared again in Japan in 1997 after a nine-year absence. Influenza B viruses evolved into three major lineages, including the earliest strain (I), B/Yamagata/16/88-like variants (II), which comprised of three sublineages (II-(i), II-(ii), II-(iii)), and B/Victoria/2/87-like variants (III). Evolution of influenza B virus hemagglutinin was apparently distinguishable from that of influenza A virus, showing a systematic mechanism of nucleotide deletion and insertion. This phenomenon was observed to be closely related to evolutionary pathways of I, II-(i), II-(ii), II-(iii) and III lineages. It was noteworthy to reveal that the nucleotide deletion and insertion mechanism of influenza B virus completed one cycle over a fifty-year period, and that a three nucleotide deletion was again observed in 1997 strains belonging to lineage II-(iii). It was evident that amino acid substitutions accompanying nucleotide insertions were highly conserved.
Asunto(s)
Glicoproteínas Hemaglutininas del Virus de la Influenza/genética , Virus de la Influenza B/clasificación , Antígenos Virales/análisis , Secuencia de Bases , Evolución Biológica , Clonación Molecular , Eliminación de Gen , Humanos , Virus de la Influenza B/genética , Virus de la Influenza B/inmunología , Gripe Humana/epidemiología , Japón/epidemiología , Datos de Secuencia MolecularRESUMEN
During the mixed epidemic caused by influenza A (H3N2) and B in the 1992-1993 season in Japan, large sequential outbreaks occurred in an institution for mentally handicapped people where none of the residents or staff members had been immunized. During the influenza A outbreak (A/ Beijing/32/92-like strain) in January, 37.0% of the residents (85/230) and 31.4% of the staff (75/239) had an influenza-like illness. During the influenza B outbreak (B/Panama/45/90- and B/Beijing/184/ 93-like strain) in late February, 59.0% of the residents and 24.3% of the staff had an influenza-like illness. As many as 25.2% of the residents had two episodes of influenza-like illness during the season, as opposed to only 5.4% of the staff members. Mixed epidemics probably have a severe impact on institutionalized high-risk people, adversely affecting them almost twice as much as influenza epidemics caused by a single virus.
Asunto(s)
Subtipo H3N2 del Virus de la Influenza A , Virus de la Influenza A/aislamiento & purificación , Virus de la Influenza B/aislamiento & purificación , Gripe Humana/virología , Adolescente , Adulto , Anciano , Personas con Discapacidad , Brotes de Enfermedades , Femenino , Humanos , Virus de la Influenza A/clasificación , Virus de la Influenza B/clasificación , Gripe Humana/epidemiología , Discapacidad Intelectual , Masculino , Persona de Mediana Edad , Características de la ResidenciaRESUMEN
In the winter of 1989 and the spring of 1990, there were large outbreaks of respiratory disease in two swine herds in Nagasaki Prefecture, southern Japan. Serological surveillance indicated that the majority of swine possessed antibodies to swine influenza virus H1 haemagglutinin and neuraminidase of early H3N2 influenza virus strains. Eight viruses were isolated from swine that showed typical clinical symptoms of influenza. The haemagglutinin and neuraminidase of these isolates were closely related to those of swine H1N1 and early human H3N2 viruses, respectively. At least two types of haemagglutinin antigens, distinguished by two monoclonal antibodies, were involved in the outbreaks. Evolutionary analyses indicated that the haemagglutinin gene of the H1N2 reassortants was closely related to those of a recent swine lineage (A/sw/HK/1/74 and A/sw/Ehime/1/80 viruses). However, the neuraminidase genes of the H1N2 reassortants were similar to those of swine N2 viruses which in turn are related to early human H3N2 viruses. A comparison of partial nucleotide sequences revealed that the six other genes of A/sw/Nagasaki/1/89 were derived from those of swine H1N1 virus.
Asunto(s)
Virus de la Influenza A , Gripe Humana/veterinaria , Virus Reordenados , Enfermedades de los Porcinos/epidemiología , Animales , Antígenos Virales/análisis , Evolución Biológica , Brotes de Enfermedades , Genoma Viral , Pruebas de Inhibición de Hemaglutinación , Glicoproteínas Hemaglutininas del Virus de la Influenza , Hemaglutininas Virales/genética , Humanos , Virus de la Influenza A/enzimología , Virus de la Influenza A/genética , Virus de la Influenza A/inmunología , Virus de la Influenza A/aislamiento & purificación , Gripe Humana/sangre , Gripe Humana/epidemiología , Gripe Humana/inmunología , Japón/epidemiología , Datos de Secuencia Molecular , Neuraminidasa/genética , Virus Reordenados/enzimología , Virus Reordenados/genética , Virus Reordenados/inmunología , Virus Reordenados/aislamiento & purificación , PorcinosRESUMEN
Two strains of influenza A virus were isolated from pigs in northern Japan in 1992. Serological tests showed that the haemagglutinin (HA) and neuraminidase (NA) antigens were more closely related to those of recent human H1N1 viruses than to those of swine H1N1 viruses. The HA and NA genes of isolate A/sw/Obihiro/5/92 were shown to be closely related to those of current human H1N1 viruses. Evolutionary trees constructed from nucleotide sequences showed that the HA and NA genes of A/sw/Obihiro/5/92 were apparently on a branch cluster containing human strains isolated between 1990 and 1992.
Asunto(s)
Subtipo H1N1 del Virus de la Influenza A , Virus de la Influenza A/inmunología , Porcinos/virología , Animales , Secuencia de Bases , Glicoproteínas Hemaglutininas del Virus de la Influenza , Hemaglutininas Virales/genética , Hemaglutininas Virales/inmunología , Virus de la Influenza A/genética , Japón , Datos de Secuencia Molecular , Neuraminidasa/genética , Neuraminidasa/inmunologíaRESUMEN
During the 1988/1989 influenza season, five antigenic reassortant influenza A (H1N2) viruses not previously isolated from man were isolated in Hebei province, People's Republic of China. All isolates contained haemagglutinins (HAs) and neuraminidases (NAs) which were antigenically similar to those of the recent Russian (H1N1) and Hong Kong influenza A (H3N2) viruses, respectively. The results of antigenic and nucleotide sequence analyses revealed that the genes encoding the polymerase, nucleoprotein, NA, matrix and non-structural proteins of the reassortant A/Hebei/24/89 (H1N2) virus were derived from the H3N2 parent virus, whereas its HA gene was from the H1N1 parent virus. The nucleotide sequences of the HA (encoding the HA1 subunit) and NA genes of the reassortant viruses were also determined. Phylogenetic trees constructed from these data by the neighbour-joining method revealed that the HA gene of the reassortant virus was closely related to those of recent human H1N1 viruses, whereas the NA gene was related to a recent human Hong Kong (H3N2) virus lineage.
Asunto(s)
Antígenos Virales/genética , Evolución Biológica , Virus de la Influenza A/inmunología , Gripe Humana/microbiología , Secuencia de Bases , China/epidemiología , ADN Viral , Brotes de Enfermedades , Genes Virales , Glicoproteínas Hemaglutininas del Virus de la Influenza , Hemaglutininas Virales/genética , Humanos , Virus de la Influenza A/enzimología , Virus de la Influenza A/genética , Gripe Humana/epidemiología , Datos de Secuencia Molecular , Neuraminidasa/genética , Homología de Secuencia de Ácido NucleicoRESUMEN
From winter 1989 to spring 1990, a severe epidemic caused by influenza A (H3N2) and B viruses developed in Japan. During the epidemic (December 1989 to February 1990), 244 children were admitted to the pediatric ward of Nippon Kokan Hospital: 53 (21.7%) were hospitalized with influenza virus infection, 22 (9.0%) with rotavirus gastroenteritis, and 17 (7.0%) with respiratory syncytial virus infection. Among those with influenza, 24 had type A and 29 had type B. Most were young healthy children without underlying illnesses (mean age, 4.8 +/- 3.4 years). The impact of the influenza epidemic on pediatric hospitalization is probably much greater than generally thought when a severe epidemic occurs.
Asunto(s)
Brotes de Enfermedades , Hospitalización/estadística & datos numéricos , Virus de la Influenza A/aislamiento & purificación , Virus de la Influenza B/aislamiento & purificación , Gripe Humana/epidemiología , Adolescente , Factores de Edad , Bronquitis/epidemiología , Niño , Preescolar , Crup/epidemiología , Femenino , Gastroenteritis/epidemiología , Hospitales con 300 a 499 Camas , Humanos , Lactante , Japón/epidemiología , Masculino , Neumonía/epidemiología , Virus Sincitiales Respiratorios/aislamiento & purificación , Infecciones por Respirovirus/epidemiología , Infecciones por Rotavirus/epidemiologíaAsunto(s)
Infecciones por Adenoviridae/diagnóstico , Infecciones por Adenovirus Humanos/diagnóstico , Adenoviridae/inmunología , Adenoviridae/aislamiento & purificación , Infecciones por Adenovirus Humanos/microbiología , Anticuerpos Antivirales/análisis , Antígenos Virales/análisis , Pruebas de Inhibición de Hemaglutinación , Humanos , Técnicas para Inmunoenzimas , Pruebas SerológicasRESUMEN
Mouse erythrocytes were used, instead of rat erythrocytes, for typing adenoviruses by hemagglutination. Mouse erythrocytes were agglutinated by the prototype strains for adenovirus types 1, 2, 4, 5, 6, 8, 9 and 10 but not by types 3 and 7. Clear hemagglutination was observed without heterotypic antiserum by the microplate technique. No significant difference was observed in the hemagglutination titer using two strains of mice. Adenoviruses were identified by the hemagglutination inhibition test, using mouse erythrocytes and prototype strains 1, 2, 4, 5, 6 and 8. The use of mouse erythrocytes for typing adenoviruses is recommended.